LOCUS       RC217270                8184 bp    DNA     circular  SYN 
DEFINITION  HELQ (Myc-DDK-tagged)-Human helicase, POLQ-like (HELQ)
ACCESSION   NM_133636
VERSION     NM_133636.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4341..4432 
                     /label="Myc-DDK"  
     misc_feature    4462..5047 
                     /label="PolyA signal"  
     misc_feature    4519..4538 
                     /label="Restriction Enzyme"  
     misc_feature    5323..5995 
                     /label="ColE1"  
     misc_feature    6425..7219 
                     /label="Kan/Neo"  
     misc_feature    7254..7556 
                     /label="SV40 ori"  
     misc_feature    7642..8119 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4323..4329 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4323 
                     /label="HELQ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAATGT GGTTCCCGCA TCCGCCGGCG GGTGTCTCTC CCCAAAAGGA 
     1081 ACCGTCCAAG CTTGGGGTGT ATTTTTGGCG CTCCCACCGC GGCCGAGCTC GAGCCCGGAG 
     1141 ATGAGGGGAA AGAGGAGGAG GAAATGGTGG CTGAGAACAG GAGGCGGAAA ACCGCGGGCG 
     1201 TACTGCCGGT TGAGGTACAG CCCCTTCTAC TCTCAGATTC CCCGGAATGT CTCGTCCTTG 
     1261 GAGGTGGTGA TACAAACCCG GACCTCCTAC GTCACATGCC CACTGACAGA GGGGTGGGAG 
     1321 ACCAGCCTAA TGACAGTGAA GTGGACATGT TTGGTGACTA TGATAGCTTT ACTGAAAACT 
     1381 CCTTTATAGC TCAAGTTGAC GACCTGGAAC AAAAATATAT GCAACTCCCT GAACATAAGA 
     1441 AACATGCTAC AGACTTTGCC ACTGAAAATC TTTGCTCGGA AAGTATCAAA AACAAACTCA 
     1501 GCATTACTAC CATAGGCAAC CTTACTGAAT TACAAACTGA TAAGCACACA GAGAACCAGA 
     1561 GTGGATATGA AGGTGTCACT ATTGAACCTG GAGCTGATCT TTTGTATGAT GTACCTTCCT 
     1621 CACAGGCTAT ATACTTTGAA AATTTGCAGA ACTCTTCAAA TGATTTGGGT GATCATTCTA 
     1681 TGAAAGAAAG GGATTGGAAG TCATCCTCTC ACAACACTGT GAATGAGGAA CTGCCCCATA 
     1741 ATTGCATAGA GCAACCCCAG CAAAATGATG AGTCCTCTTC CAAAGTCAGA ACTAGTTCAG 
     1801 ATATGAACAG GAGAAAAAGT ATTAAAGATC ATCTAAAAAA TGCCATGACT GGAAATGCGA 
     1861 AGGCCCAGAC ACCAATATTT TCTAGAAGTA AACAGCTCAA AGACACTCTC CTATCTGAGG 
     1921 AAATTAATGT TGCTAAGAAA ACAGTTGAGT CATCATCAAA TGACCTTGGT CCTTTTTATT 
     1981 CATTACCCAG CAAAGTGAGA GACCTTTATG CCCAATTCAA GGGAATTGAA AAATTATATG 
     2041 AATGGCAACA TACTTGTTTA ACATTGAATT CTGTGCAAGA AAGAAAAAAT TTAATATATT 
     2101 CCTTGCCAAC AAGTGGTGGA AAAACCCTCG TGGCTGAGAT TTTAATGCTG CAAGAACTGC 
     2161 TTTGCTGTCG GAAAGATGTT TTAATGATTC TTCCATATGT GGCAATTGTC CAAGAAAAGA 
     2221 TTTCAGGTTT GTCAAGTTTT GGTATAGAAC TCGGTTTCTT TGTTGAAGAA TATGCTGGAA 
     2281 GCAAAGGAAG ATTTCCTCCA ACTAAAAGAA GGGAAAAGAA ATCACTCTAT ATTGCCACTA 
     2341 TTGAAAAAGG ACATAGCTTG GTGAACTCCT TGATTGAAAC TGGAAGAATT GACAGTCTGG 
     2401 GTCTGGTTGT TGTAGACGAG TTGCACATGA TTGGTGAAGG AAGCCGTGGA GCTACACTGG 
     2461 AAATGACCCT AGCAAAAATC CTCTACACTA GCAAAACGAC TCAAATTATT GGTATGAGTG 
     2521 CAACATTAAA CAATGTTGAA GACCTACAAA AGTTTCTTCA AGCAGAATAT TATACCAGTC 
     2581 AATTTAGACC AGTTGAGTTA AAAGAATATC TGAAAATAAA TGATACAATA TATGAAGTTG 
     2641 ACAGCAAAGC TGAGAATGGC ATGACTTTTT CACGTCTTCT TAATTATAAG TATTCTGATA 
     2701 CCCTAAAAAA GATGGATCCT GATCACTTGG TAGCATTGGT GACAGAAGTT ATTCCCAATT 
     2761 ATTCCTGCTT AGTTTTTTGT CCTAGTAAGA AGAACTGTGA AAATGTAGCA GAAATGATAT 
     2821 GCAAATTTTT AAGCAAGGAA TATCTGAAAC ATAAGGAGAA AGAAAAATGT GAGGTGATTA 
     2881 AGAACTTGAA GAATATTGGC AATGGCAACC TGTGTCCTGT TTTAAAGCGC ACTATCCCAT 
     2941 TTGGAGTTGC CTATCACCAC AGTGGCTTAA CAAGTGATGA AAGGAAACTC TTGGAGGAGG 
     3001 CCTACTCCAC AGGAGTGCTC TGTCTTTTTA CCTGCACATC TACCCTAGCG GCAGGTGTCA 
     3061 ACCTACCAGC TCGAAGAGTT ATTTTAAGAG CTCCCTATGT TGCTAAGGAA TTTTTAAAGA 
     3121 GGAATCAATA TAAACAGATG ATTGGCAGAG CTGGTCGTGC TGGAATAGAT ACTATTGGGG 
     3181 AGAGTATCCT CATATTGCAA GAAAAAGACA AACAACAGGT ATTGGAGTTA ATAACTAAAC 
     3241 CATTGGAAAA CTGTTACAGC CATCTTGTTC AGGAATTCAC CAAGGGAATC CAAACATTAT 
     3301 TTCTCTCTTT GATTGGTTTG AAGATTGCAA CGAATCTTGA TGACATCTAT CATTTCATGA 
     3361 ATGGTACATT TTTTGGTGTT CAGCAAAAGG TTTTATTGAA AGAAAAAAGT CTCTGGGAAA 
     3421 TAACTGTTGA ATCACTTAGA TACCTGACAG AAAAAGGACT CCTACAAAAA GACACTATTT 
     3481 ATAAGTCTGA AGAAGAGGTC CAATATAATT TTCATATTAC AAAGTTGGGA CGTGCTTCAT 
     3541 TTAAGGGAAC TATAGATTTA GCTTATTGTG ACATTCTGTA CAGAGACTTG AAGAAAGGTC 
     3601 TTGAAGGACT TGTGCTTGAA AGCCTTCTTC ATCTAATCTA CCTAACAACC CCCTATGATC 
     3661 TGGTTTCACA GTGTAACCCT GATTGGATGA TATACTTCAG GCAGTTTAGC CAACTCAGTC 
     3721 CAGCAGAACA AAATGTAGCT GCCATTCTTG GAGTCTCTGA AAGCTTTATT GGGAAGAAAG 
     3781 CATCAGGCCA AGCCATCGGA AAGAAGGTGG ACAAGAACGT TGTCAACAGG CTATATCTGT 
     3841 CTTTTGTTCT TTATACCTTG CTCAAAGAGA CCAACATTTG GACTGTATCT GAAAAATTTA 
     3901 ATATGCCTCG AGGATATATA CAAAATCTTC TCACTGGAAC TGCCTCATTC TCATCTTGTG 
     3961 TGTTACATTT CTGTGAGGAG CTTGAGGAGT TTTGGGTTTA CAGAGCCCTT TTGGTAGAAC 
     4021 TTACCAAGAA GCTGACTTAC TGTGTAAAGG CAGAATTAAT CCCTCTCATG GAAGTTACTG 
     4081 GAGTTTTAGA GGGTCGAGCA AAACAGTTAT ACAGTGCAGG TTACAAAAGT CTAATGCACT 
     4141 TAGCTAATGC AAATCCTGAA GTGCTCGTAA GGACAATTGA TCATTTATCA AGACGCCAAG 
     4201 CCAAGCAAAT TGTTTCATCA GCAAAGATGC TGTTGCATGA AAAAGCAGAA GCCCTGCAAG 
     4261 AAGAGGTAGA AGAGTTACTA AGATTGCCTT CTGATTTCCC TGGTGCTGTG GCTTCTTCCA 
     4321 CTGACAAAGC AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4381 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4441 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4501 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4561 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4621 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4681 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4741 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4801 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4861 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4921 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4981 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5041 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5101 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5161 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5221 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5281 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5341 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5401 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5461 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5521 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5581 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5641 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5701 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5761 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5821 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5881 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5941 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6001 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6061 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6121 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6181 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6241 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6301 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6361 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6421 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6481 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6541 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6601 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6661 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6721 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6781 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6841 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6901 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6961 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7021 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7081 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7141 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7201 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7261 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7321 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7381 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7621 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7681 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7741 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7801 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7861 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7921 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7981 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8041 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8101 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8161 TAACAAAAAT TTAACGCGAA TTTT
//