LOCUS       RC217233                7890 bp    DNA     circular  SYN 
DEFINITION  OPA1 (Myc-DDK-tagged)-Human optic atrophy 1 (autosomal dominant) (OPA1), nuclear gene encoding mitochondrial protein, transcript variant 7
ACCESSION   NM_130836
VERSION     NM_130836.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4045..4136 
                     /label="Myc-DDK"  
     misc_feature    4166..4751 
                     /label="PolyA signal"  
     misc_feature    4223..4242 
                     /label="Restriction Enzyme"  
     misc_feature    5027..5699 
                     /label="ColE1"  
     misc_feature    6129..6923 
                     /label="Kan/Neo"  
     misc_feature    6958..7260 
                     /label="SV40 ori"  
     misc_feature    7346..7823 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4020..4027 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4020 
                     /label="OPA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGACTA CGTCGGGCCG CTGTGGCCTG TGAGGTCTGC CAGTCTTTAG 
     1081 TGAAACACAG CTCTGGAATA AAAGGAAGTT TACCACTACA AAAACTACAT CTGGTTTCAC 
     1141 GAAGCATTTA TCATTCACAT CATCCTACCT TAAAGCTTCA ACGACCCCAA TTAAGGACAT 
     1201 CCTTTCAGCA GTTCTCTTCT CTGACAAACC TTCCTTTACG TAAACTGAAA TTCTCTCCAA 
     1261 TTAAATATGG CTACCAGCCT CGCAGGAATT TTTGGCCAGC AAGATTAGCT ACGAGACTCT 
     1321 TAAAACTTCG CTATCTCATA CTAGGATCGG CTGTTGGGGG TGGCTACACA GCCAAAAAGA 
     1381 CTTTTGATCA GTGGAAAGAT ATGATACCGG ACCTTAGTGA ATATAAATGG ATTGTGCCTG 
     1441 ACATTGTGTG GGAAATTGAT GAGTATATCG ATTTTGAGAA AATTAGAAAA GCCCTTCCTA 
     1501 GTTCAGAAGA CCTTGTAAAG TTAGCACCAG ACTTTGACAA GATTGTTGAA AGCCTTAGCT 
     1561 TATTGAAGGA CTTTTTTACC TCAGGTTCTC CGGAAGAAAC GGCGTTTAGA GCAACAGATC 
     1621 GTGGATCTGA AAGTGACAAG CATTTTAGAA AGGGTCTGCT TGGTGAGCTC ATTCTCTTAC 
     1681 AACAACAAAT TCAAGAGCAT GAAGAGGAAG CGCGCAGAGC CGCTGGCCAA TATAGCACGA 
     1741 GCTATGCCCA ACAGAAGCGC AAGGTGTCAG ACAAAGAGAA AATTGACCAA CTTCAGGAAG 
     1801 AACTTCTGCA CACTCAGTTG AAGTATCAGA GAATCTTGGA ACGATTAGAA AAGGAGAACA 
     1861 AAGAATTGAG AAAATTAGTA TTGCAGAAAG ATGACAAAGG CATTCATCAT AGAAAGCTTA 
     1921 AGAAATCTTT GATTGACATG TATTCTGAAG TTCTTGATGT TCTCTCTGAT TATGATGCCA 
     1981 GTTATAATAC GCAAGATCAT CTGCCACGGG TTGTTGTGGT TGGAGATCAG AGTGCTGGAA 
     2041 AGACTAGTGT GTTGGAAATG ATTGCCCAAG CTCGAATATT CCCAAGAGGA TCTGGGGAGA 
     2101 TGATGACACG TTCTCCAGTT AAGGTGACTC TGAGTGAAGG TCCTCACCAT GTGGCCCTAT 
     2161 TTAAAGATAG TTCTCGGGAG TTTGATCTTA CCAAAGAAGA AGATCTTGCA GCATTAAGAC 
     2221 ATGAAATAGA ACTTCGAATG AGGAAAAATG TGAAAGAAGG CTGTACCGTT AGCCCTGAGA 
     2281 CCATATCCTT AAATGTAAAA GGCCCTGGAC TACAGAGGAT GGTGCTTGTT GACTTACCAG 
     2341 GTGTGATTAA TACTGTGACA TCAGGCATGG CTCCTGACAC AAAGGAAACT ATTTTCAGTA 
     2401 TCAGCAAAGC TTACATGCAG AATCCTAATG CCATCATACT GTGTATTCAA GATGGATCTG 
     2461 TGGATGCTGA ACGCAGTATT GTTACAGACT TGGTCAGTCA AATGGACCCT CATGGAAGGA 
     2521 GAACCATATT CGTTTTGACC AAAGTAGACC TGGCAGAGAA AAATGTAGCC AGTCCAAGCA 
     2581 GGATTCAGCA GATAATTGAA GGAAAGCTCT TCCCAATGAA AGCTTTAGGT TATTTTGCTG 
     2641 TTGTAACAGG AAAAGGGAAC AGCTCTGAAA GCATTGAAGC TATAAGAGAA TATGAAGAAG 
     2701 AGTTTTTTCA GAATTCAAAG CTCCTAAAGA CAAGCATGCT AAAGGCACAC CAAGTGACTA 
     2761 CAAGAAATTT AAGCCTTGCA GTATCAGACT GCTTTTGGAA AATGGTACGA GAGTCTGTTG 
     2821 AACAACAGGC TGATAGTTTC AAAGCAACAC GTTTTAACCT TGAAACTGAA TGGAAGAATA 
     2881 ACTATCCTCG CCTGCGGGAA CTTGACCGGA ATGAACTATT TGAAAAAGCT AAAAATGAAA 
     2941 TCCTTGATGA AGTTATCAGT CTGAGCCAGG TTACACCAAA ACATTGGGAG GAAATCCTTC 
     3001 AACAATCTTT GTGGGAAAGA GTATCAACTC ATGTGATTGA AAACATCTAC CTTCCAGCTG 
     3061 CGCAGACCAT GAATTCAGGA ACTTTTAACA CCACAGTGGA TATCAAGCTT AAACAGTGGA 
     3121 CTGATAAACA ACTTCCTAAT AAAGCAGTAG AGGTTGCTTG GGAGACCCTA CAAGAAGAAT 
     3181 TTTCCCGCTT TATGACAGAA CCGAAAGGGA AAGAGCATGA TGACATATTT GATAAACTTA 
     3241 AAGAGGCTGT TAAGGAAGAA AGTATTAAAC GACACAAGTG GAATGACTTT GCGGAGGACA 
     3301 GCTTGAGGGT TATTCAACAC AATGCTTTGG AAGACCGATC CATATCTGAT AAACAGCAAT 
     3361 GGGATGCAGC TATTTATTTT ATGGAAGAGG CTCTGCAGGC TCGTCTCAAG GATACTGAAA 
     3421 ATGCAATTGA AAACATGGTG GGTCCAGACT GGAAAAAGAG GTGGTTATAC TGGAAGAATC 
     3481 GGACCCAAGA ACAGTGTGTT CACAATGAAA CCAAGAATGA ATTGGAGAAG ATGTTGAAAT 
     3541 GTAATGAGGA GCACCCAGCT TATCTTGCAA GTGATGAAAT AACCACAGTC CGGAAGAACC 
     3601 TTGAATCCCG AGGAGTAGAA GTAGATCCAA GCTTGATTAA GGATACTTGG CATCAAGTTT 
     3661 ATAGAAGACA TTTTTTAAAA ACAGCTCTAA ACCATTGTAA CCTTTGTCGA AGAGGTTTTT 
     3721 ATTACTACCA AAGGCATTTT GTAGATTCTG AGTTGGAATG CAATGATGTG GTCTTGTTTT 
     3781 GGCGTATACA GCGCATGCTT GCTATCACCG CAAATACTTT AAGGCAACAA CTTACAAATA 
     3841 CTGAAGTTAG GCGATTAGAG AAAAATGTTA AAGAGGTATT GGAAGATTTT GCTGAAGATG 
     3901 GTGAGAAGAA GATTAAATTG CTTACTGGTA AACGCGTTCA ACTGGCGGAA GACCTCAAGA 
     3961 AAGTTAGAGA AATTCAAGAA AAACTTGATG CTTTCATTGA AGCTCTTCAT CAGGAGAAAA 
     4021 GCGGACCGAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4081 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4141 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4201 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4261 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4321 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4381 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4441 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4501 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4561 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4621 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4681 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4741 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4801 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4861 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4921 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4981 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5041 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5101 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5161 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5221 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5281 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5341 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5401 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5461 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5521 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5581 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5641 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5701 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5761 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5821 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5881 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5941 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6001 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6061 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6121 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6181 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6241 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6301 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6361 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6421 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6481 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6541 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6601 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6661 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6721 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6781 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6841 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6901 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6961 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7021 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7081 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7141 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7201 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7321 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7381 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7441 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7501 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7561 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7621 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7681 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7741 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7801 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7861 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//