LOCUS       RC217224                8769 bp    DNA     circular  SYN 
DEFINITION  PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1c'
ACCESSION   NM_001083605
VERSION     NM_001083605.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4926..5017 
                     /label="Myc-DDK"  
     misc_feature    5047..5632 
                     /label="PolyA signal"  
     misc_feature    5104..5123 
                     /label="Restriction Enzyme"  
     misc_feature    5908..6580 
                     /label="ColE1"  
     misc_feature    7010..7804 
                     /label="Kan/Neo"  
     misc_feature    7839..8141 
                     /label="SV40 ori"  
     misc_feature    8227..8704 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4908..4914 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4908 
                     /label="PTCH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTAATCCT CAACTCATGA TACAGACCCC TAAAGAAGAA GGTGCTAATG 
     1081 TCCTGACCAC AGAAGCGCTC CTACAACACC TGGACTCGGC ACTCCAGGCC AGCCGTGTCC 
     1141 ATGTATACAT GTACAACAGG CAGTGGAAAT TGGAACATTT GTGTTACAAA TCAGGAGAGC 
     1201 TTATCACAGA AACAGGTTAC ATGGATCAGA TAATAGAATA TCTTTACCCT TGTTTGATTA 
     1261 TTACACCTTT GGACTGCTTC TGGGAAGGGG CGAAATTACA GTCTGGGACA GCATACCTCC 
     1321 TAGGTAAACC TCCTTTGCGG TGGACAAACT TCGACCCTTT GGAATTCCTG GAAGAGTTAA 
     1381 AGAAAATAAA CTATCAAGTG GACAGCTGGG AGGAAATGCT GAATAAGGCT GAGGTTGGTC 
     1441 ATGGTTACAT GGACCGCCCC TGCCTCAATC CGGCCGATCC AGACTGCCCC GCCACAGCCC 
     1501 CCAACAAAAA TTCAACCAAA CCTCTTGATA TGGCCCTTGT TTTGAATGGT GGATGTCATG 
     1561 GCTTATCCAG AAAGTATATG CACTGGCAGG AGGAGTTGAT TGTGGGTGGC ACAGTCAAGA 
     1621 ACAGCACTGG AAAACTCGTC AGCGCCCATG CCCTGCAGAC CATGTTCCAG TTAATGACTC 
     1681 CCAAGCAAAT GTACGAGCAC TTCAAGGGGT ACGAGTATGT CTCACACATC AACTGGAACG 
     1741 AGGACAAAGC GGCAGCCATC CTGGAGGCCT GGCAGAGGAC ATATGTGGAG GTGGTTCATC 
     1801 AGAGTGTCGC ACAGAACTCC ACTCAAAAGG TGCTTTCCTT CACCACCACG ACCCTGGACG 
     1861 ACATCCTGAA ATCCTTCTCT GACGTCAGTG TCATCCGCGT GGCCAGCGGC TACTTACTCA 
     1921 TGCTCGCCTA TGCCTGTCTA ACCATGCTGC GCTGGGACTG CTCCAAGTCC CAGGGTGCCG 
     1981 TGGGGCTGGC TGGCGTCCTG CTGGTTGCAC TGTCAGTGGC TGCAGGACTG GGCCTGTGCT 
     2041 CATTGATCGG AATTTCCTTT AACGCTGCAA CAACTCAGGT TTTGCCATTT CTCGCTCTTG 
     2101 GTGTTGGTGT GGATGATGTT TTTCTTCTGG CCCACGCCTT CAGTGAAACA GGACAGAATA 
     2161 AAAGAATCCC TTTTGAGGAC AGGACCGGGG AGTGCCTGAA GCGCACAGGA GCCAGCGTGG 
     2221 CCCTCACGTC CATCAGCAAT GTCACAGCCT TCTTCATGGC CGCGTTAATC CCAATTCCCG 
     2281 CTCTGCGGGC GTTCTCCCTC CAGGCAGCGG TAGTAGTGGT GTTCAATTTT GCCATGGTTC 
     2341 TGCTCATTTT TCCTGCAATT CTCAGCATGG ATTTATATCG ACGCGAGGAC AGGAGACTGG 
     2401 ATATTTTCTG CTGTTTTACA AGCCCCTGCG TCAGCAGAGT GATTCAGGTT GAACCTCAGG 
     2461 CCTACACCGA CACACACGAC AATACCCGCT ACAGCCCCCC ACCTCCCTAC AGCAGCCACA 
     2521 GCTTTGCCCA TGAAACGCAG ATTACCATGC AGTCCACTGT CCAGCTCCGC ACGGAGTACG 
     2581 ACCCCCACAC GCACGTGTAC TACACCACCG CTGAGCCGCG CTCCGAGATC TCTGTGCAGC 
     2641 CCGTCACCGT GACACAGGAC ACCCTCAGCT GCCAGAGCCC AGAGAGCACC AGCTCCACAA 
     2701 GGGACCTGCT CTCCCAGTTC TCCGACTCCA GCCTCCACTG CCTCGAGCCC CCCTGTACGA 
     2761 AGTGGACACT CTCATCTTTT GCTGAGAAGC ACTATGCTCC TTTCCTCTTG AAACCAAAAG 
     2821 CCAAGGTAGT GGTGATCTTC CTTTTTCTGG GCTTGCTGGG GGTCAGCCTT TATGGCACCA 
     2881 CCCGAGTGAG AGACGGGCTG GACCTTACGG ACATTGTACC TCGGGAAACC AGAGAATATG 
     2941 ACTTTATTGC TGCACAATTC AAATACTTTT CTTTCTACAA CATGTATATA GTCACCCAGA 
     3001 AAGCAGACTA CCCGAATATC CAGCACTTAC TTTACGACCT ACACAGGAGT TTCAGTAACG 
     3061 TGAAGTATGT CATGTTGGAA GAAAACAAAC AGCTTCCCAA AATGTGGCTG CACTACTTCA 
     3121 GAGACTGGCT TCAGGGACTT CAGGATGCAT TTGACAGTGA CTGGGAAACC GGGAAAATCA 
     3181 TGCCAAACAA TTACAAGAAT GGATCAGACG ATGGAGTCCT TGCCTACAAA CTCCTGGTGC 
     3241 AAACCGGCAG CCGCGATAAG CCCATCGACA TCAGCCAGTT GACTAAACAG CGTCTGGTGG 
     3301 ATGCAGATGG CATCATTAAT CCCAGCGCTT TCTACATCTA CCTGACGGCT TGGGTCAGCA 
     3361 ACGACCCCGT CGCGTATGCT GCCTCCCAGG CCAACATCCG GCCACACCGA CCAGAATGGG 
     3421 TCCACGACAA AGCCGACTAC ATGCCTGAAA CAAGGCTGAG AATCCCGGCA GCAGAGCCCA 
     3481 TCGAGTATGC CCAGTTCCCT TTCTACCTCA ACGGCTTGCG GGACACCTCA GACTTTGTGG 
     3541 AGGCAATTGA AAAAGTAAGG ACCATCTGCA GCAACTATAC GAGCCTGGGG CTGTCCAGTT 
     3601 ACCCCAACGG CTACCCCTTC CTCTTCTGGG AGCAGTACAT CGGCCTCCGC CACTGGCTGC 
     3661 TGCTGTTCAT CAGCGTGGTG TTGGCCTGCA CATTCCTCGT GTGCGCTGTC TTCCTTCTGA 
     3721 ACCCCTGGAC GGCCGGGATC ATTGTGATGG TCCTGGCGCT GATGACGGTC GAGCTGTTCG 
     3781 GCATGATGGG CCTCATCGGA ATCAAGCTCA GTGCCGTGCC CGTGGTCATC CTGATCGCTT 
     3841 CTGTTGGCAT AGGAGTGGAG TTCACCGTTC ACGTTGCTTT GGCCTTTCTG ACGGCCATCG 
     3901 GCGACAAGAA CCGCAGGGCT GTGCTTGCCC TGGAGCACAT GTTTGCACCC GTCCTGGATG 
     3961 GCGCCGTGTC CACTCTGCTG GGAGTGCTGA TGCTGGCGGG ATCTGAGTTC GACTTCATTG 
     4021 TCAGGTATTT CTTTGCTGTG CTGGCGATCC TCACCATCCT CGGCGTTCTC AATGGGCTGG 
     4081 TTTTGCTTCC CGTGCTTTTG TCTTTCTTTG GACCATATCC TGAGGTGTCT CCAGCCAACG 
     4141 GCTTGAACCG CCTGCCCACA CCCTCCCCTG AGCCACCCCC CAGCGTGGTC CGCTTCGCCA 
     4201 TGCCGCCCGG CCACACGCAC AGCGGGTCTG ATTCCTCCGA CTCGGAGTAT AGTTCCCAGA 
     4261 CGACAGTGTC AGGCCTCAGC GAGGAGCTTC GGCACTACGA GGCCCAGCAG GGCGCGGGAG 
     4321 GCCCTGCCCA CCAAGTGATC GTGGAAGCCA CAGAAAACCC CGTCTTCGCC CACTCCACTG 
     4381 TGGTCCATCC CGAATCCAGG CATCACCCAC CCTCGAACCC GAGACAGCAG CCCCACCTGG 
     4441 ACTCAGGGTC CCTGCCTCCC GGACGGCAAG GCCAGCAGCC CCGCAGGGAC CCCCCCAGAG 
     4501 AAGGCTTGTG GCCACCCCCC TACAGACCGC GCAGAGACGC TTTTGAAATT TCTACTGAAG 
     4561 GGCATTCTGG CCCTAGCAAT AGGGCCCGCT GGGGCCCTCG CGGGGCCCGT TCTCACAACC 
     4621 CTCGGAACCC AGCGTCCACT GCCATGGGCA GCTCCGTGCC CGGCTACTGC CAGCCCATCA 
     4681 CCACTGTGAC GGCTTCTGCC TCCGTGACTG TCGCCGTGCA CCCGCCGCCT GTCCCTGGGC 
     4741 CTGGGCGGAA CCCCCGAGGG GGACTCTGCC CAGGCTACCC TGAGACTGAC CACGGCCTGT 
     4801 TTGAGGACCC CCACGTGCCT TTCCACGTCC GGTGTGAGAG GAGGGATTCG AAGGTGGAAG 
     4861 TCATTGAGCT GCAGGACGTG GAATGCGAGG AGAGGCCCCG GGGAAGCAGC TCCAACACGC 
     4921 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4981 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5041 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5101 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5161 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5221 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5281 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5341 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5401 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5461 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5521 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5581 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5641 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5701 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5761 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5821 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5881 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5941 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6001 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6061 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6121 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6181 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6241 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6301 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6361 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6421 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6481 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6541 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6601 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6661 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6721 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6781 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6841 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6901 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6961 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7021 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7081 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7141 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7201 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7261 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7321 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7381 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7441 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7501 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7561 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7621 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7681 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7741 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7801 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7861 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7921 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7981 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8221 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8281 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8341 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8401 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8461 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8521 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8581 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8641 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8701 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8761 GCGAATTTT
//