LOCUS       RC217200                7767 bp    DNA     circular  SYN 
DEFINITION  PCSK6 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 8
ACCESSION   NM_138321
VERSION     NM_138321.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3924..4015 
                     /label="Myc-DDK"  
     misc_feature    4045..4630 
                     /label="PolyA signal"  
     misc_feature    4102..4121 
                     /label="Restriction Enzyme"  
     misc_feature    4906..5578 
                     /label="ColE1"  
     misc_feature    6008..6802 
                     /label="Kan/Neo"  
     misc_feature    6837..7139 
                     /label="SV40 ori"  
     misc_feature    7225..7702 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3906..3912 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3906 
                     /label="PCSK6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCTG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGCCGCCAC CGACACCGCC GCGGGCGCGG GGGGCGCGGG GGGCGCGGGG GGCGCCGGCG 
     1141 GGCCCGGGTT CCGGCCGCTC GCGCCGCGTC CCTGGCGCTG GCTGCTGCTG CTGGCGCTGC 
     1201 CTGCCGCCTG CTCCGCGCCC CCGCCGCGCC CCGTCTACAC CAACCACTGG GCGGTGCAAG 
     1261 TGCTGGGCGG CCCGGCCGAG GCGGACCGCG TGGCGGCGGC GCACGGGTAC CTCAACTTGG 
     1321 GCCAGATTGG AAACCTGGAA GATTACTACC ATTTTTATCA CAGCAAAACC TTTAAAAGAT 
     1381 CAACCTTGAG TAGCAGAGGC CCTCACACCT TCCTCAGAAT GGACCCCCAG GTGAAATGGC 
     1441 TCCAGCAACA GGAAGTGAAA CGAAGGGTGA AGAGACAGGT GCGAAGTGAC CCGCAGGCCC 
     1501 TTTACTTCAA CGACCCCATT TGGTCCAACA TGTGGTACCT GCATTGTGGC GACAAGAACA 
     1561 GTCGCTGCCG GTCGGAAATG AATGTCCAGG CAGCGTGGAA GAGGGGCTAC ACAGGAAAAA 
     1621 ACGTGGTGGT CACCATCCTT GATGATGGCA TAGAGAGAAA TCACCCTGAC CTGGCCCCAA 
     1681 ATTATGATTC CTACGCCAGC TACGACGTGA ACGGCAATGA TTATGACCCA TCTCCACGAT 
     1741 ATGATGCCAG CAATGAAAAT AAACACGGCA CTCGTTGTGC GGGAGAAGTT GCTGCTTCAG 
     1801 CAAACAATTC CTACTGCATC GTGGGCATAG CGTACAATGC CAAAATAGGA GGCATCCGCA 
     1861 TGCTGGACGG CGATGTCACA GATGTGGTCG AGGCAAAGTC GCTGGGCATC AGACCCAACT 
     1921 ACATCGACAT TTACAGTGCC AGCTGGGGGC CGGACGACGA CGGCAAGACG GTGGACGGGC 
     1981 CCGGCCGACT GGCTAAGCAG GCTTTCGAGT ATGGCATTAA AAAGGGCCGG CAGGGCCTGG 
     2041 GCTCCATTTT CGTCTGGGCA TCTGGGAATG GCGGGAGAGA GGGGGACTAC TGCTCGTGCG 
     2101 ATGGCTACAC CAACAGCATC TACACCATCT CCGTCAGCAG CGCCACCGAG AATGGCTACA 
     2161 AGCCCTGGTA CCTGGAAGAG TGTGCCTCCA CCCTGGCCAC CACCTACAGC AGTGGGGCCT 
     2221 TTTATGAGCG AAAAATCGTC ACCACGGATC TGCGTCAGCG CTGTACCGAT GGCCACACTG 
     2281 GGACCTCAGT CTCTGCCCCC ATGGTGGCGG GCATCATCGC CTTGGCTCTA GAAGCAAACA 
     2341 GCCAGTTAAC CTGGAGGGAC GTCCAGCACC TGCTAGTGAA GACATCCCGG CCGGCCCACC 
     2401 TGAAAGCGAG CGACTGGAAA GTGAACGGCG CGGGTCATAA AGTTAGCCAT TTCTATGGAT 
     2461 TTGGTTTGGT GGACGCAGAA GCTCTCGTTG TGGAGGCAAA GAAGTGGACA GCAGTGCCAT 
     2521 CGCAGCACAT GTGTGTGGCC GCCTCGGACA AGAGACCCAG GAGCATCCCC TTAGTGCAGG 
     2581 TGCTGCGGAC TACGGCCCTG ACCAGCGCCT GCGCGGAGCA CTCGGACCAG CGGGTGGTCT 
     2641 ACTTGGAGCA CGTGGTGGTT CGCACCTCCA TCTCACACCC ACGCCGAGGA GACCTCCAGA 
     2701 TCTACCTGGT TTCTCCCTCG GGAACCAAGT CTCAACTTCT GGCAAAGAGG TTGCTGGATC 
     2761 TTTCCAATGA AGGGTTTACA AACTGGGAAT TCATGACTGT CCACTGCTGG GGAGAAAAGG 
     2821 CTGAAGGGCA GTGGACCTTG GAAATCCAAG ATCTGCCATC CCAGGTCCGC AACCCGGAGA 
     2881 AGCAAGGGAA GTTGAAAGAA TGGAGCCTCA TACTGTATGG CACAGCAGAG CACCCGTACC 
     2941 ACACCTTCAG TGCCCATCAG TCCCGCTCGC GGATGCTGGA GCTCTCAGCC CCAGAGCTGG 
     3001 AGCCACCCAA GGCTGCCCTG TCACCCTCCC AGGTGGAAGT TCCTGAAGAT GAGGAAGATT 
     3061 ACACAGGTGT GTGCCATCCG GAGTGTGGTG ACAAAGGCTG TGATGGCCCC AATGCAGACC 
     3121 AGTGCTTGAA CTGCGTCCAC TTCAGCCTGG GGAGTGTCAA GACCAGCAGG AAGTGCGTGA 
     3181 GTGTGTGCCC CTTGGGCTAC TTTGGGGACA CAGCAGCAAG ACGCTGTCGC CGGTGCCACA 
     3241 AGGGGTGTGA GACCTGCTCC AGCAGAGCTG CGACGCAGTG CCTGTCTTGC CGCCGCGGGT 
     3301 TCTATCACCA CCAGGAGATG AACACCTGTG TGACCCTCTG TCCTGCAGGA TTTTATGCTG 
     3361 ATGAAAGTCA GAAAAATTGC CTTAAATGCC ACCCAAGCTG TAAAAAGTGC GTGGATGAAC 
     3421 CTGAGAAATG TACTGTCTGT AAAGAAGGAT TCAGCCTTGC ACGGGGCAGC TGCATTCCTG 
     3481 ACTGTGAGCC AGGCACCTAC TTTGACTCAG AGCTGATCAG ATGTGGGGAA TGCCATCACA 
     3541 CCTGCGGAAC CTGCGTGGGG CCAGGCAGAG AAGAGTGCAT TCACTGTGCG AAAAACTTCC 
     3601 ACTTCCACGA CTGGAAGTGT GTGCCAGCCT GTGGTGAGGG CTTCTACCCA GAAGAGATGC 
     3661 CGGGCTTGCC CCACAAAGTG TGTCGAAGGT ACGGTCCTCC TGGCGGGGAA CGGCAGGCAG 
     3721 CTGTGTCCAG CAAAGGAGTG CCTGGAGGGC AGAGTCTGGC AGCATCTTCC CCAGGGGCCG 
     3781 GGGAGGGCAT GTTGCATCAC CCCACTGTGG ACAGGAGCCC ATTTACAGAG CTGCTGAGGG 
     3841 GCCTTCGTCC CTTTGTTCAC TGGATGCATA TTTGTTGGGT CCCTGCTGTG GGCAGACACC 
     3901 GTGCAGCAGC GGGAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3961 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4021 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4081 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4141 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4201 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4261 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4321 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4381 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4441 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4501 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4561 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4621 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4681 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4741 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4801 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4861 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4921 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4981 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5041 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5101 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5161 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5221 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5281 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5341 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5401 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5461 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5521 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5581 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5641 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5701 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5761 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5821 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5881 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5941 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6001 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6061 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6121 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6181 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6241 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6301 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6361 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6421 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6481 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6541 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6601 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6661 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6721 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6781 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6841 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6901 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6961 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7021 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7081 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7141 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7201 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7261 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7321 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7381 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7441 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7501 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7561 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7621 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7681 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7741 ATTTAACAAA AATTTAACGC GAATTTT
//