LOCUS       RC217162                8304 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK-tagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 3
ACCESSION   NM_206820
VERSION     NM_206820.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GACTGGACCC 
     1141 TTGTCGAAAC TCCTCCTGGG GAGGAACAAG CCAAGCAGAA TGCCAACTCC CAGCTGTCCA 
     1201 TCTTGTTCAT TGAAAAACCT CAAGGAGGAA CAGTGAAAGT TGGTGAAGAT ATCACCTTCA 
     1261 TAGCCAAAGT CAAGGCTGAA GATCTTCTGA GAAAACCCAC TATCAAATGG TTCAAAGGAA 
     1321 AATGGATGGA CCTGGCCAGC AAAGCCGGGA AGCACCTTCA GCTGAAGGAA ACCTTTGAGA 
     1381 GGCACAGTCG GGTGTACACA TTTGAGATGC AGATCATCAA GGCCAAAGAT AACTTTGCAG 
     1441 GAAATTACAG ATGCGAGGTC ACCTATAAGG ATAAGTTTGA CAGCTGTTCA TTTGATCTTG 
     1501 AAGTGCACGA ATCTACTGGG ACTACTCCAA ACATTGACAT CAGATCTGCT TTCAAGAGAA 
     1561 GTGGAGAAGG TCAAGAGGAT GCAGGAGAAC TTGACTTTAG TGGTCTCCTG AAACGTAGGG 
     1621 AGGTGAAGCA GCAGGAGGAA GAACCCCAGG TGGACGTATG GGAGTTGCTG AAGAACGCGA 
     1681 AACCCAGTGA GTACGAGAAG ATCGCCTTCC AGTATGGAAT CACCGACCTG CGCGGCATGC 
     1741 TCAAGCGACT CAAGCGCATG CGCAGAGAGG AGAAGAAGAG CGCAGCTTTT GCAAAAATTC 
     1801 TTGATCCTGC ATATCAGGTT GACAAAGGAG GCAGAGTGAG GTTTGTTGTG GAGCTGGCAG 
     1861 ATCCAAAGTT GGAGGTGAAA TGGTATAAAA ATGGTCAAGA AATTCGACCC AGTACCAAAT 
     1921 ACATCTTTGA ACACAAAGGA TGCCAGAGAA TCCTGTTTAT CAATAACTGT CAGATGACAG 
     1981 ATGATTCAGA GTATTATGTG ACAGCCGGTG ATGAGAAATG TTCCACTGAG CTCTTCGTAA 
     2041 GAGAGCCTCC AATTATGGTG ACCAAACAGC TGGAAGATAC AACTGCTTAT TGTGGGGAGA 
     2101 GAGTGGAATT AGAATGTGAG GTGTCTGAAG ATGATGCCAA TGTAAAATGG TTTAAGAATG 
     2161 GTGAAGAGAT TATCCCTGGT CCAAAATCAA GATACCGAAT TAGAGTTGAG GGTAAAAAAC 
     2221 ACATCTTGAT CATAGAGGGA GCAACAAAGG CTGATGCTGC AGAATATTCA GTAATGACAA 
     2281 CAGGAGGACA ATCATCTGCT AAACTTAGTG TTGACTTGAA ACCTCTGAAG ATTTTGACAC 
     2341 CTCTGACTGA TCAGACTGTA AATCTTGGAA AAGAAATCTG CCTGAAGTGT GAAATCTCTG 
     2401 AAAACATACC AGGAAAATGG ACTAAAAATG GCCTACCTGT TCAGGAGAGT GACCGTCTAA 
     2461 AGGTGGTTCA CAAGGGAAGG ATCCACAAGT TAGTGATAGC CAATGCCCTC ACTGAAGATG 
     2521 AAGGTGATTA TGTATTTGCA CCTGATGCCT ACAATGTTAC TCTGCCTGCC AAAGTTCATG 
     2581 TTATTGATCC TCCTAAGATC ATCCTGGATG GTCTTGATGC TGACAACACA GTGACAGTGA 
     2641 TTGCAGGAAA CAAGCTTCGT CTTGAGATCC CCATCAGCGG AGAACCACCT CCTAAAGCCA 
     2701 TGTGGAGCCG GGGAGATAAG GCTATTATGG AAGGCAGTGG CCGGATAAGA ACAGAATCTT 
     2761 ACCCTGATAG CAGCACTCTG GTCATTGATA TAGCTGAAAG AGATGACTCT GGTGTTTACC 
     2821 ACATCAATCT GAAAAACGAA GCTGGAGAGG CACATGCAAG CATCAAGGTT AAAGTTGTGG 
     2881 ACTTCCCTGA TCCTCCAGTG GCACCGACTG TGACAGAGGT GGGAGATGAC TGGTGTATCA 
     2941 TGAACTGGGA GCCTCCTGCC TACGACGGAG GCTCTCCAAT CCTAGGATAT TTTATTGAGA 
     3001 GGAAGAAGAA ACAAAGCTCC AGGTGGATGA GGCTGAATTT TGATCTCTGC AAAGAAACAA 
     3061 CTTTTGAGCC CAAGAAGATG ATTGAAGGTG TGGCCTATGA GGTCCGCATC TTTGCAGTCA 
     3121 ATGCCATTGG CATCTCCAAG CCCAGTATGC CCTCCAGGCC TTTTGTTCCT TTGGCTGTAA 
     3181 CAAGCCCTCC TACTCTTCTG ACTGTGGACT CTGTCACTGA CACGACTGTC ACGATGAGGT 
     3241 GGCGCCCCCC AGACCACATT GGTGCAGCAG GTTTAGATGG CTATGTGCTA GAGTATTGCT 
     3301 TTGAAGGAAG TACATCAGCA AAACAGTCTG ATGAAAATGG GGAGGCTGCC TATGATCTGC 
     3361 CAGCTGAGGA CTGGATAGTT GCAAACAAAG ATCTGATTGA CAAGACGAAG TTCACCATCA 
     3421 CAGGTCTGCC AACAGATGCA AAGATCTTTG TGCGTGTGAA GGCTGTTAAT GCAGCTGGTG 
     3481 CCAGCGAGCC CAAGTACTAT TCTCAGCCCA TTCTCGTGAA GGAAATCATA GAACCTCCAA 
     3541 AGATTCGCAT TCCAAGACAC CTGAAGCAAA CCTATATCCG CAGAGTTGGA GAAGCTGTCA 
     3601 ATCTGGTTAT ACCTTTCCAG GGAAAACCAA GACCAGAATT AACTTGGAAG AAGGATGGTG 
     3661 CAGAAATTGA TAAGAATCAA ATAAACATTC GCAACTCTGA GACTGATACA ATCATATTTA 
     3721 TTAGAAAAGC AGAGAGGAGC CACTCTGGGA AATATGATCT GCAAGTCAAA GTGGACAAAT 
     3781 TCGTGGAGAC CGCATCAATT GACATCCAGA TCATTGACCG TCCAGGTCCA CCCCAAATTG 
     3841 TGAAGATTGA GGATGTCTGG GGAGAAAATG TCGCTCTCAC ATGGACTCCA CCAAAGGATG 
     3901 ATGGAAATGC TGCTATCACA GGCTATACCA TTCAGAAGGC TGACAAGAAG AGCATGGAAT 
     3961 GGTTTACTGT CATTGAGCAT TATCATCGAA CCAGTGCCAC CATTACTGAA TTGGTCATAG 
     4021 GGAATGAATA TTACTTCCGG GTCTTTTCTG AAAACATGTG TGGCCTCAGT GAGGATGCCA 
     4081 CCATGACTAA AGAGAGTGCA GTGATCGCCA GGGATGGTAA AATCTACAAA AATCCAGTGT 
     4141 ATGAAGACTT TGATTTCTCA GAGGCACCCA TGTTTACTCA GCCTTTGGTT AACACCTATG 
     4201 CCATAGCTGG TTACAATGCC ACCCTAAACT GCAGTGTGAG AGGAAATCCT AAGCCTAAAA 
     4261 TAACCTGGAT GAAAAACAAA GTTGCTATTG TGGATGATCC AAGATACAGG ATGTTCAGCA 
     4321 ACCAGGGAGT CTGTACCCTG GAAATTCGCA AGCCCAGCCC CTATGATGGA GGCACTTACT 
     4381 GCTGCAAAGC AGTCAATGAC CTTGGGACAG TGGAGATTGA ATGCAAACTG GAGGTGAAAG 
     4441 TCATTGCACA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//