LOCUS       RC217131                9384 bp    DNA     circular  SYN 
DEFINITION  ARHGAP5 (Myc-DDK-tagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2
ACCESSION   NM_001173
VERSION     NM_001173.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5541..5632 
                     /label="Myc-DDK"  
     misc_feature    5662..6247 
                     /label="PolyA signal"  
     misc_feature    5719..5738 
                     /label="Restriction Enzyme"  
     misc_feature    6523..7195 
                     /label="ColE1"  
     misc_feature    7625..8419 
                     /label="Kan/Neo"  
     misc_feature    8454..8756 
                     /label="SV40 ori"  
     misc_feature    8842..9319 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5523..5529 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5523 
                     /label="ARHGAP5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCAAAA AACAAAGAGC CTCGTCCCCC ATCCTATACC ATCAGTATAG 
     1081 TTGGACTCTC TGGGACTGAA AAAGACAAAG GTAACTGTGG AGTTGGAAAG TCTTGTTTGT 
     1141 GCAATAGATT TGTACGCTCA AAAGCAGATG AATATTATCC AGAGCATACT TCTGTGCTTA 
     1201 GCACCATTGA CTTTGGAGGA CGAGTAGTAA ACAATGATCA CTTTTTGTAC TGGGGTGACA 
     1261 TAATACAAAA TAGTGAAGAT GGAGTAGAAT GCAAAATTCA TGTCATTGAA CAAACAGAGT 
     1321 TCATTGATGA CCAGACTTTC TTGCCTCATC GGAGTACGAA TTTGCAACCA TATATAAAAC 
     1381 GTGCAGCTGC ATCTAAATTG CAGTCAGCAG AAAAACTAAT GTACATTTGC ACTGATCAGC 
     1441 TAGGCTTAGA ACAAGACTTT GAACAGAAGC AAATGCCTGA AGGGAAGCTC AACGTAGATG 
     1501 GATTTTTATT ATGCATTGAT GTAAGTCAAG GATGCAATAG GAAGTTTGAT GATCAACTTA 
     1561 AATTTGTGAA TAACCTTTTT GTCCAGTTAT CAAAATCAAA AAAACCTGTA ATAATAGCAG 
     1621 CAACTAAATG TGATGAATGC GTGGATCATT ATCTTAGAGA AGTTCAGGCA TTTGCTTCAA 
     1681 ATAAAAAGAA CCTTCTTGTA GTGGAAACAT CAGCACGATT TAATGTCAAC ATTGAAACAT 
     1741 GTTTTACTGC ACTGGTACAA ATGTTGGATA AAACTCGTAG CAAGCCTAAA ATTATTCCCT 
     1801 ATTTGGATGC TTATAAAACA CAGAGACAAC TTGTTGTCAC AGCAACAGAT AAGTTTGAAA 
     1861 AACTTGTGCA GACTGTGAGA GATTATCATG CAACTTGGAA AACTGTTAGT AATAAATTAA 
     1921 AAAATCATCC TGATTATGAA GAATACATCA ACTTAGAGGG AACAAGAAAG GCCAGAAATA 
     1981 CATTCTCAAA ACATATAGAA CAACTTAAAC AGGAACATAT AAGAAAAAGG AGAGAAGAGT 
     2041 ATATAAATAC TTTACCAAGA GCTTTTAACA CTCTTTTGCC AAATCTAGAA GAGATTGAAC 
     2101 ATTTGAATTG GTCAGAAGCT TTGAAGTTAA TGGAAAAGAG AGCAGATTTC CAGTTATGTT 
     2161 TTGTGGTGCT AGAAAAAACT CCTTGGGATG AAACTGACCA TATAGACAAA ATTAATGATA 
     2221 GGCGGATTCC ATTTGACCTC CTGAGCACTT TAGAAGCTGA AAAAGTCTAT CAGAACCATG 
     2281 TACAGCATCT GATATCCGAG AAGAGGAGGG TGGAAATGAA GGAAAAATTC AAAAAGACTT 
     2341 TGGAAAAAAT TCAATTCATT TCACCAGGGC AGCCATGGGA GGAAGTTATG TGCTTTGTTA 
     2401 TGGAGGATGA AGCCTACAAA TATATCACTG AGGCTGATAG CAAAGAGGTA TATGGTAGGC 
     2461 ATCAGCGAGA AATAGTTGAA AAAGCCAAAG AAGAGTTTCA AGAAATGCTT TTTGAGCATT 
     2521 CTGAACTTTT TTATGATTTA GATCTTAATG CAACACCTAG TTCAGATAAA ATGAGTGAAA 
     2581 TTCATACAGT TCTGAGTGAA GAACCTAGAT ATAAAGCTTT ACAGAAACTT GCACCTGATA 
     2641 GGGAATCCCT TCTACTTAAG CATATAGGAT TTGTTTATCA TCCCACTAAA GAAACATGTC 
     2701 TTAGTGGCCA AAATTGTACA GACATTAAAG TGGAGCAGTT ACTTGCTAGT AGTCTTTTAC 
     2761 AGTTGGATCA TGGCCGCTTA AGATTATATC ACGATAGTAC CAATATAGAT AAAGTTAACC 
     2821 TTTTTATTTT AGGGAAGGAT GGCCTTGCCC AAGAACTAGC AAATGAGATA AGGACACAAT 
     2881 CCACTGATGA TGAGTATGCC TTAGATGGAA AAATTTATGA ACTTGATCTT CGGCCGGTTG 
     2941 ATGCCAAATC GCCTTACTTT TTGAGTCAGT TATGGACTGC CGCCTTTAAA CCACATGGGT 
     3001 GCTTCTGTGT ATTTAATTCC ATTGAGTCAT TGAGTTTTAT TGGGGAATTT ATTGGGAAAA 
     3061 TAAGAACTGA AGCTTCTCAG ATCAGAAAAG ATAAATACAT GGCTAATCTT CCATTTACAT 
     3121 TAATTCTGGC TAATCAGAGA GATTCCATTA GTAAGAATCT ACCAATTCTC AGGCACCAAG 
     3181 GGCAGCAGTT GGCAAACAAG TTGCAATGTC CTTTTGTAGA TGTACCTGCT GGTACATATC 
     3241 CTCGTAAATT TAATGAAACC CAAATAAAGC AAGCTCTCAG AGGAGTATTG GAATCAGTTA 
     3301 AACACAATTT GGATGTGGTG AGCCCAATTC CTGCCAATAA GGACTTATCA GAAGCTGACT 
     3361 TGAGAATTGT CATGTGCGCC ATGTGTGGAG ATCCATTTAG TGTGGATCTT ATTCTTTCAC 
     3421 CCTTCCTTGA TTCTCATTCT TGCAGTGCTG CTCAAGCTGG ACAGAATAAT TCCCTAATGC 
     3481 TTGATAAAAT CATTGGTGAA AAAAGGAGGC GAATACAGAT CACAATATTA TCATACCACT 
     3541 CTTCAATTGG AGTAAGAAAA GATGAACTAG TTCATGGGTA TATATTAGTT TACTCTGCAA 
     3601 AACGGAAAGC TTCGATGGGA ATGCTTCGAG CATTTCTATC AGAAGTTCAA GACACCATTC 
     3661 CTGTACAGCT GGTGGCAGTT ACTGACAGCC AAGCAGATTT TTTTGAAAAT GAGGCTATCA 
     3721 AAGAGTTAAT GACTGAAGGA GAACACATTG CAACTGAGAT CACTGCTAAA TTTACAGCAC 
     3781 TGTATTCTTT ATCTCAGTAT CATCGGCAAA CTGAGGTCTT TACTCTGTTT TTTAGTGATG 
     3841 TTCTAGAGAA AAAAAATATG ATAGAAAATT CTTATTTGTC TGATAATACA AGGGAATCAA 
     3901 CCCATCAAAG TGAAGATGTT TTTCTACCAT CTCCCAGAGA CTGTTTTCCC TATAATAACT 
     3961 ACCCTGATTC AGATGATGAC ACAGAAGCAC CACCTCCTTA TAGTCCAATT GGGGATGATG 
     4021 TACAGTTGCT TCCAACACCT AGTGACCGTT CCAGATATAG ATTAGATTTG GAAGGAAATG 
     4081 AGTATCCTAT TCATAGTACC CCAAACTGTC ATGACCATGA ACGCAACCAT AAAGTGCCTC 
     4141 CACCTATTAA ACCTAAACCA GTTGTACCTA AGACAAATGT GAAAAAACTC GATCCAAACC 
     4201 TTTTAAAAAC AATTGAAGCT GGTATTGGTA AAAATCCAAG AAAGCAGACT TCCCGGGTGC 
     4261 CTTTGGCACA TCCTGAAGAT ATGGATCCTT CAGATAACTA TGCGGAACCC ATTGATACAA 
     4321 TTTTCAAACA GAAGGGCTAT TCTGATGAGA TTTATGTTGT CCCAGATGAT AGTCAAAATC 
     4381 GTATTAAAAT TCGAAACTCA TTTGTAAATA ACACCCAAGG AGATGAAGAA AATGGGTTTT 
     4441 CTGATAGAAC CTCAAAAAGT CATGGGGAAC GGAGGCCTTC AAAATACAAA TATAAATCTA 
     4501 AAACCTTGTT TAGTAAAGCC AAGTCATACT ATAGAAGAAC ACATTCAGAT GCCAGTGATG 
     4561 ATGAGGCTTT CACCACTTCT AAAACAAAAA GAAAAGGAAG ACATCGTGGA AGTGAAGAAG 
     4621 ATCCACTTCT TTCTCCTGTT GAAACTTGGA AAGGTGGTAT TGATAATCCT GCAATCACTT 
     4681 CTGACCAGGA GTTAGATGAT AAGAAGATGA AGAAGAAAAC CCACAAAGTG AAAGAAGATA 
     4741 AAAAGAAAAA GAAAACTAAG AACTTCAATC CACCAACACG TAGAAATTGG GAAAGTAATT 
     4801 ACTTTGGGAT GCCCCTCCAG GATCTGGTTA CAGCTGAGAA GCCCATACCA CTATTTGTTG 
     4861 AGAAATGTGT GGAATTTATT GAAGATACAG GGTTATGTAC CGAAGGACTC TACCGTGTCA 
     4921 GCGGGAATAA AACTGACCAA GACAATATTC AAAAGCAGTT TGATCAAGAT CATAATATCA 
     4981 ATCTAGTGTC AATGGAAGTA ACAGTAAATG CTGTAGCTGG AGCCCTTAAA GCTTTCTTTG 
     5041 CAGATCTGCC AGATCCTTTA ATTCCATATT CTCTTCATCC AGAACTATTG GAAGCAGCAA 
     5101 AAATCCCGGA TAAAACAGAA CGTCTTCATG CCTTGAAAGA AATTGTTAAG AAATTTCATC 
     5161 CTGTAAACTA TGATGTATTC AGATACGTGA TAACACATCT AAACAGGGTT AGTCAGCAAC 
     5221 ATAAAATCAA CCTAATGACA GCAGACAACT TATCCATCTG TTTTTGGCCA ACCTTGATGA 
     5281 GACCTGATTT TGAAAATCGA GAGTTTCTGT CTACTACTAA GATTCATCAA TCTGTTGTTG 
     5341 AAACATTCAT TCAGCAGTGT CAGTTTTTCT TTTACAATGG AGAAATTGTA GAAACGACAA 
     5401 ACATTGTGGC TCCTCCACCA CCTTCAAACC CAGGACAGTT GGTGGAACCA ATGGTGCCAC 
     5461 TTCAGTTGCC GCCACCATTG CAACCTCAGC TGATACAACC ACAATTACAA ACGGATCCTC 
     5521 TTGGTATTAT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5581 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5641 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5701 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5761 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5821 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5881 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5941 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     6001 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     6061 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     6121 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6181 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6241 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6301 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6361 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6421 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6481 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6541 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6601 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6661 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6721 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6781 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6841 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6901 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6961 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     7021 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7081 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7141 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7201 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7261 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7321 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7381 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7441 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7501 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7561 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7621 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7681 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7741 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7801 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7861 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7921 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7981 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     8041 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     8101 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     8161 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8221 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8281 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8341 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8401 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8461 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8521 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8581 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8641 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8701 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8761 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8821 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8881 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8941 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     9001 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     9061 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     9121 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9181 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9241 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9301 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9361 TAACAAAAAT TTAACGCGAA TTTT
//