LOCUS RC217128 7788 bp DNA circular SYN DEFINITION PCSK6 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 1 ACCESSION NM_002570 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3945..4036 /label="Myc-DDK" misc_feature 4066..4651 /label="PolyA signal" misc_feature 4123..4142 /label="Restriction Enzyme" misc_feature 4927..5599 /label="ColE1" misc_feature 6029..6823 /label="Kan/Neo" misc_feature 6858..7160 /label="SV40 ori" misc_feature 7246..7723 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3927..3933 /label="Restriction Enzyme" misc_feature 1020..3927 /label="PCSK6" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCTG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 1081 CCGCCGCCAC CGACACCGCC GCGGGCGCGG GGGGCGCGGG GGGCGCGGGG GGCGCCGGCG 1141 GGCCCGGGTT CCGGCCGCTC GCGCCGCGTC CCTGGCGCTG GCTGCTGCTG CTGGCGCTGC 1201 CTGCCGCCTG CTCCGCGCCC CCGCCGCGCC CCGTCTACAC CAACCACTGG GCGGTGCAAG 1261 TGCTGGGCGG CCCGGCCGAG GCGGACCGCG TGGCGGCGGC GCACGGGTAC CTCAACTTGG 1321 GCCAGATTGG AAACCTGGAA GATTACTACC ATTTTTATCA CAGCAAAACC TTTAAAAGAT 1381 CAACCTTGAG TAGCAGAGGC CCTCACACCT TCCTCAGAAT GGACCCCCAG GTGAAATGGC 1441 TCCAGCAACA GGAAGTGAAA CGAAGGGTGA AGAGACAGGT GCGAAGTGAC CCGCAGGCCC 1501 TTTACTTCAA CGACCCCATT TGGTCCAACA TGTGGTACCT GCATTGTGGC GACAAGAACA 1561 GTCGCTGCCG GTCGGAAATG AATGTCCAGG CAGCGTGGAA GAGGGGCTAC ACAGGAAAAA 1621 ACGTGGTGGT CACCATCCTT GATGATGGCA TAGAGAGAAA TCACCCTGAC CTGGCCCCAA 1681 ATTATGATTC CTACGCCAGC TACGACGTGA ACGGCAATGA TTATGACCCA TCTCCACGAT 1741 ATGATGCCAG CAATGAAAAT AAACACGGCA CTCGTTGTGC GGGAGAAGTT GCTGCTTCAG 1801 CAAACAATTC CTACTGCATC GTGGGCATAG CGTACAATGC CAAAATAGGA GGCATCCGCA 1861 TGCTGGACGG CGATGTCACA GATGTGGTCG AGGCAAAGTC GCTGGGCATC AGACCCAACT 1921 ACATCGACAT TTACAGTGCC AGCTGGGGGC CGGACGACGA CGGCAAGACG GTGGACGGGC 1981 CCGGCCGACT GGCTAAGCAG GCTTTCGAGT ATGGCATTAA AAAGGGCCGG CAGGGCCTGG 2041 GCTCCATTTT CGTCTGGGCA TCTGGGAATG GCGGGAGAGA GGGGGACTAC TGCTCGTGCG 2101 ATGGCTACAC CAACAGCATC TACACCATCT CCGTCAGCAG CGCCACCGAG AATGGCTACA 2161 AGCCCTGGTA CCTGGAAGAG TGTGCCTCCA CCCTGGCCAC CACCTACAGC AGTGGGGCCT 2221 TTTATGAGCG AAAAATCGTC ACCACGGATC TGCGTCAGCG CTGTACCGAT GGCCACACTG 2281 GGACCTCAGT CTCTGCCCCC ATGGTGGCGG GCATCATCGC CTTGGCTCTA GAAGCAAACA 2341 GCCAGTTAAC CTGGAGGGAC GTCCAGCACC TGCTAGTGAA GACATCCCGG CCGGCCCACC 2401 TGAAAGCGAG CGACTGGAAA GTGAACGGCG CGGGTCATAA AGTTAGCCAT TTCTATGGAT 2461 TTGGTTTGGT GGACGCAGAA GCTCTCGTTG TGGAGGCAAA GAAGTGGACA GCAGTGCCAT 2521 CGCAGCACAT GTGCGTGGCC GCCTCGGACA AGAGACCCAG GAGCATCCCC TTAGTGCAGG 2581 TGCTGCGGAC TACGGCCCTG ACCAGCGCCT GCGCGGAGCA CTCGGACCAG CGGGTGGTCT 2641 ACTTGGAGCA CGTGGTGGTT CGCACCTCCA TCTCACACCC ACGCCGAGGA GACCTCCAGA 2701 TCTACCTGGT TTCTCCCTCG GGAACCAAGT CTCAACTTTT GGCAAAGAGG TTGCTGGATC 2761 TTTCCAATGA AGGGTTTACA AACTGGGAAT TCATGACTGT CCACTGCTGG GGAGAAAAGG 2821 CTGAAGGGCA GTGGACCTTG GAAATCCAAG ATCTGCCATC CCAGGTCCGC AACCCGGAGA 2881 AGCAAGGGAA GTTGAAAGAA TGGAGCCTCA TACTGTATGG CACAGCAGAG CACCCGTACC 2941 ACACCTTCAG TGCCCATCAG TCCCGCTCGC GGATGCTGGA GCTCTCAGCC CCAGAGCTGG 3001 AGCCACCCAA GGCTGCCCTG TCACCCTCCC AGGTGGAAGT TCCTGAAGAT GAGGAAGATT 3061 ACACAGCTCA ATCCACCCCA GGCTCTGCTA ATATTTTACA GACCAGTGTG TGCCATCCGG 3121 AGTGTGGTGA CAAAGGCTGT GATGGCCCCA ATGCAGACCA GTGCTTGAAC TGCGTCCACT 3181 TCAGCCTGGG GAGTGTCAAG ACCAGCAGGA AGTGCGTGAG TGTGTGCCCC TTGGGCTACT 3241 TTGGGGACAC AGCAGCAAGA CGCTGTCGCC GGTGCCACAA GGGGTGTGAG ACCTGCTCCA 3301 GCAGAGCTGC GACGCAGTGC CTGTCTTGCC GCCGCGGGTT CTATCACCAC CAGGAGATGA 3361 ACACCTGTGT GACCCTCTGT CCTGCAGGAT TTTATGCTGA TGAAAGTCAG AAAAATTGCC 3421 TTAAATGCCA CCCAAGCTGT AAAAAGTGCG TGGATGAACC TGAGAAATGT ACTGTCTGTA 3481 AAGAAGGATT CAGCCTTGCA CGGGGCAGCT GCATTCCTGA CTGTGAGCCA GGCACCTACT 3541 TTGACTCAGA GCTGATCAGA TGTGGGGAAT GCCATCACAC CTGCGGAACC TGCGTGGGGC 3601 CAGGCAGAGA AGAGTGCATT CACTGTGCGA AAAACTTCCA CTTCCACGAC TGGAAGTGTG 3661 TGCCAGCCTG TGGTGAGGGC TTCTACCCAG AAGAGATGCC GGGCTTGCCC CACAAAGTGT 3721 GTCGAAGGTG TGACGAGAAC TGCTTGAGCT GTGCAGGCTC CAGCAGGAAC TGTAGCAGGT 3781 GTAAGACGGG CTTCACACAG CTGGGGACCT CCTGCATCAC CAACCACACG TGCAGCAACG 3841 CTGACGAGAC ATTCTGCGAG ATGGTGAAGT CCAACCGGCT GTGCGAACGG AAGCTCTTCA 3901 TTCAGTTCTG CTGCCGCACG TGCCTCCTGG CCGGGACGCG TACGCGGCCG CTCGAGCAGA 3961 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 4021 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 4081 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 4141 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 4201 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 4261 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 4321 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 4381 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 4441 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 4501 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 4561 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 4621 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 4681 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 4741 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 4801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 4861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 4921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 4981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 5041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 5101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 5161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 5221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 5281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 5341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 5401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 5461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 5521 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 5581 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 5641 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 5701 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 5761 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 5821 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 5881 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 5941 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 6001 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 6061 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 6121 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 6181 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 6241 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 6301 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 6361 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 6421 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 6481 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 6541 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 6601 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 6661 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 6721 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 6781 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 6841 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 6901 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 6961 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 7021 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 7201 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 7261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 7321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 7381 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 7441 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 7501 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 7561 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 7621 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 7681 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 7741 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT //