LOCUS RC217084L4 8759 bp DNA circular SYN DEFINITION HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class I, F (HLA-F), transcript variant 2 ACCESSION NM_018950 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7048..7228 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 7956..8602 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 5923..5943 /label="LR50" misc_feature 4386..5114 /label="mGFP" misc_feature 5121..5186 /label="P2A" misc_feature 7276..7895 /label="pBR322 Ori" misc_feature 5187..5786 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 4358..4365 /label="Restriction Enzyme" misc_feature 3320..4358 /label="HLA-F" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGCCCCGA AGCCTCCTCC TGCTGCTCTC 3361 AGGGGCCCTG GCCCTGACCG ATACTTGGGC GGGCTCCCAC TCCTTGAGGT ATTTCAGCAC 3421 CGCTGTGTCG CGGCCCGGCC GCGGGGAGCC CCGCTACATC GCCGTGGAGT ACGTAGACGA 3481 CACGCAATTC CTGCGGTTCG ACAGCGACGC CGCGATTCCG AGGATGGAGC CGCGGGAGCC 3541 GTGGGTGGAG CAAGAGGGGC CGCAGTATTG GGAGTGGACC ACAGGGTACG CCAAGGCCAA 3601 CGCACAGACT GACCGAGTGG CCCTGAGGAA CCTGCTCCGC CGCTACAACC AGAGCGAGGC 3661 TGGGTCTCAC ACCCTCCAGG GAATGAATGG CTGCGACATG GGGCCCGACG GACGCCTCCT 3721 CCGCGGGTAT CACCAGCACG CGTACGACGG CAAGGATTAC ATCTCCCTGA ACGAGGACCT 3781 GCGCTCCTGG ACCGCGGCGG ACACCGTGGC TCAGATCACC CAGCGCTTCT ATGAGGCAGA 3841 GGAATATGCA GAGGAGTTCA GGACCTACCT GGAGGGCGAG TGCCTGGAGT TGCTCCGCAG 3901 ATACTTGGAG AATGGGAAGG AGACGCTACA GCGCGCAGAT CCTCCAAAGG CACACGTTGC 3961 CCACCACCCC ATCTCTGACC ATGAGGCCAC CCTGAGGTGC TGGGCCCTGG GCTTCTACCC 4021 TGCGGAGATC ACGCTGACCT GGCAGCGGGA TGGGGAGGAA CAGACCCAGG ACACAGAGCT 4081 TGTGGAGACC AGGCCTGCAG GGGATGGAAC CTTCCAGAAG TGGGCCGCTG TGGTGGTGCC 4141 TTCTGGAGAG GAACAGAGAT ACACATGCCA TGTGCAGCAC GAGGGGCTGC CCCAGCCCCT 4201 CATCCTGAGA TGGGAGCAGT CTCCCCAGCC CACCATCCCC ATCGTGGGCA TCGTTGCTGG 4261 CCTTGTTGTC CTTGGAGCTG TGGTCACTGG AGCTGTGGTC GCTGCTGTGA TGTGGAGGAA 4321 GAAGAGCTCA GATAGAAACA GAGGGAGCTA CTCTCAGGCT GCAGTGAGCG GACCGACGCG 4381 TACGCGGCCG CTCGAGATGA GCGGGGGCGA GGAGCTGTTC GCCGGCATCG TGCCCGTGCT 4441 GATCGAGCTG GACGGCGACG TGCACGGCCA CAAGTTCAGC GTGCGCGGCG AGGGCGAGGG 4501 CGACGCCGAC TACGGCAAGC TGGAGATCAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT 4561 GCCCTGGCCC ACCCTGGTGA CCACCCTCTG CTACGGCATC CAGTGCTTCG CCCGCTACCC 4621 CGAGCACATG AAGATGAACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT ACATCCAGGA 4681 GCGCACCATC CAGTTCCAGG ACGACGGCAA GTACAAGACC CGCGGCGAGG TGAAGTTCGA 4741 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCAAG GACTTCAAGG AGGACGGCAA 4801 CATCCTGGGC CACAAGCTGG AGTACAGCTT CAACAGCCAC AACGTGTACA TCCGCCCCGA 4861 CAAGGCCAAC AACGGCCTGG AGGCTAACTT CAAGACCCGC CACAACATCG AGGGCGGCGG 4921 CGTGCAGCTG GCCGACCACT ACCAGACCAA CGTGCCCCTG GGCGACGGCC CCGTGCTGAT 4981 CCCCATCAAC CACTACCTGA GCACTCAGAC CAAGATCAGC AAGGACCGCA ACGAGGCCCG 5041 CGACCACATG GTGCTCCTGG AGTCCTTCAG CGCCTGCTGC CACACCCACG GCATGGACGA 5101 GCTGTACAGG TCCGGACTCA GAGTTTGGGT AGGAAGCGGA GCTACTAACT TCAGCCTGCT 5161 GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTATG ACCGAGTACA AGCCCACGGT 5221 GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 5281 CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGATCGC CACATCGAGC GGGTCACCGA 5341 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 5401 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 5461 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 5521 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 5581 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 5641 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 5701 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 5761 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATGT ACACCCAAAC GGCCGGCCGC 5821 GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG 5881 AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG 5941 ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 6001 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 6061 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 6121 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 6181 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 6241 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 6301 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 6361 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 6421 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 6481 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC 6541 GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT 6601 ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC 6661 ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT 6721 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT 6781 GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG 6841 CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA 6901 GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG 6961 CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA 7021 TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG 7081 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 7141 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 7201 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG 7261 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 7321 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 7381 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 7441 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 7501 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 7561 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 7621 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 7681 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 7741 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 7801 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 7861 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 7921 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT 7981 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC 8041 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT 8101 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA 8161 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT 8221 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA 8281 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT 8341 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG 8401 CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT 8461 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG 8521 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC 8581 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC 8641 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 8701 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC //