LOCUS       RC217081                9417 bp    DNA     circular  SYN 
DEFINITION  CDK13 (Myc-DDK-tagged)-Human cyclin-dependent kinase 13 (CDK13), transcript variant 1
ACCESSION   NM_003718
VERSION     NM_003718.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5574..5665 
                     /label="Myc-DDK"  
     misc_feature    5695..6280 
                     /label="PolyA signal"  
     misc_feature    5752..5771 
                     /label="Restriction Enzyme"  
     misc_feature    6556..7228 
                     /label="ColE1"  
     misc_feature    7658..8452 
                     /label="Kan/Neo"  
     misc_feature    8487..8789 
                     /label="SV40 ori"  
     misc_feature    8875..9352 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5556..5562 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5556 
                     /label="CDK13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGAGCAGC TCGGACACGG CGCTGGGGGG AGGCGGGGGC CTGAGCTGGG 
     1081 CGGAGAAGAA GTTGGAGGAA CGCCGCAAGC GGAGGCGATT CCTGTCCCCT CGGCAGCCGC 
     1141 CGCTGCTGTT GCCGCTCCTG CAGCCGCAGC TCCTGCAACC GCCGCCGCCC CCGCCGCCTC 
     1201 TGCTCTTCCT GGCTGCTCCC GGCACGGCCG CCGCCGCAGC CGCCGCCGCC GCGGCCTCCT 
     1261 CCTCTTGCTT CAGCCCGGGC CCCCCTCTGG GGGTCAAGCG GCTGGCGAGA GGCAGGGGGC 
     1321 GCGCAGGAGG GCGGCGGAAG CGGCGTCGCG GGCCCCGCGC CGGGCAGGAG GCGGAGAAGC 
     1381 GTCGGGTCTT CTCGCTGCCC CAGCCGCAGC AGGACGGCGG TGGCGGTGCT AGTAGCGGCG 
     1441 GGGGTGTGAC CCCGCTGGTG GAATACGAGG ATGTGAGCTC CCAGTCCGAG CAGGGGCTGC 
     1501 TGCTGGGGGG GGCCAGCGCG GCAACGGCGG CGACGGCTGC CGGGGGAACG GGGGGCAGCG 
     1561 GCGGGAGTCC GGCCTCCTCC TCCGGCACCC AGCGGCGCGG GGAGGGGTCG GAGCGCAGGC 
     1621 CCCGCCGGGA CCGCCGCAGC AGCAGTGGCC GCAGCAAGGA GCGCCACCGC GAGCACCGGC 
     1681 GGCGGGATGG GCAGCGCGGT GGCAGCGAGG CCTCCAAGTC CCGCAGCCGC CACAGCCACA 
     1741 GCGGCGAGGA ACGGGCCGAG GTCGCCAAGA GCGGCAGCAG CAGCAGCAGC GGCGGCCGCC 
     1801 GGAAAAGCGC TTCGGCCACA TCCAGCAGCA GTAGCAGCCG CAAGGACCGG GACTCGAAGG 
     1861 CCCACCGCAG CCGGACTAAG TCGTCCAAGG AGCCGCCTTC GGCCTACAAG GAACCGCCCA 
     1921 AGGCCTACCG GGAGGACAAG ACCGAGCCTA AGGCCTACAG GCGGCGGCGG TCCCTCAGCC 
     1981 CACTGGGAGG CCGGGACGAC AGCCCGGTGT CCCACAGGGC CTCTCAGAGC CTGAGGAGCC 
     2041 GCAAGTCCCC CAGCCCGGCA GGAGGTGGCA GCAGCCCCTA TTCTCGGCGG CTGCCGCGCT 
     2101 CCCCGAGCCC CTACAGTCGC CGCCGCTCCC CCAGCTACAG CCGCCACAGC TCCTACGAGC 
     2161 GGGGCGGCGA CGTGTCCCCT AGTCCCTACA GCAGCAGCAG CTGGCGCCGC TCTCGCAGTC 
     2221 CCTACAGCCC TGTGCTCAGA CGGTCTGGAA AATCCCGAAG CAGAAGCCCG TATTCATCTA 
     2281 GGCATTCAAG ATCTCGTAGC AGGCACAGAT TGTCTAGATC CAGAAGTCGT CATTCTAGTA 
     2341 TTTCTCCTAG CACACTAACT CTGAAGAGTA GCCTGGCAGC TGAATTGAAC AAGAATAAAA 
     2401 AAGCACGAGC AGCAGAGGCA GCAAGAGCCG CAGAAGCAGC GAAAGCTGCA GAAGCAACTA 
     2461 AGGCTGCTGA GGCTGCTGCC AAGGCTGCAA AAGCTTCAAA CACTTCTACA CCTACCAAGG 
     2521 GGAACGCGGA AACTAGTGCC AGTGCATCAC AAACAAACCA TGTGAAGGAT GTGAAGAAAA 
     2581 TTAAAATTGA ACATGCACCT TCTCCCTCAA GTGGTGGAAC TTTAAAAAAT GACAAAGCAA 
     2641 AAACAAAGCC ACCTCTTCAG GTAACGAAGG TGGAAAATAA TTTGATTGTA GATAAAGCCA 
     2701 CCAAGAAAGC AGTCATAGTT GGAAAGGAGA GTAAATCTGC TGCTACAAAG GAGGAATCAG 
     2761 TATCTCTTAA AGAGAAAACC AAACCACTTA CACCAAGCAT AGGAGCCAAG GAGAAGGAGC 
     2821 AACATGTAGC TTTAGTCACC TCTACATTAC CACCGTTACC TTTGCCTCCC ATGCTGCCTG 
     2881 AAGATAAAGA AGCTGATAGC TTACGAGGAA ATATTTCAGT AAAAGCAGTT AAAAAAGAAG 
     2941 TAGAAAAGAA ACTCCGATGT CTTCTTGCTG ATTTACCGCT GCCCCCTGAG CTACCAGGAG 
     3001 GAGATGATCT TTCAAAGAGT CCAGAGGAAA AGAAAACAGC AACACAGTTA CATAGTAAAA 
     3061 GGAGGCCTAA AATATGTGGG CCTCGCTATG GTGAAACCAA AGAAAAAGAT ATTGACTGGG 
     3121 GAAAACGCTG CGTGGATAAA TTTGATATCA TCGGAATTAT TGGAGAAGGT ACTTACGGAC 
     3181 AAGTTTACAA AGCCAGGGAT AAAGACACTG GAGAAATGGT AGCCTTAAAA AAAGTACGTC 
     3241 TGGATAATGA AAAGGAAGGC TTTCCAATTA CAGCAATTCG AGAAATTAAA ATTCTCCGGC 
     3301 AGCTTACCCA TCAGAGTATT ATCAATATGA AGGAAATAGT GACTGATAAA GAAGATGCTT 
     3361 TGGATTTCAA GAAGGACAAA GGTGCATTTT ATCTGGTGTT TGAATATATG GACCATGATC 
     3421 TGATGGGACT ACTGGAATCA GGCTTGGTTC ATTTTAATGA AAATCACATA AAGTCATTTA 
     3481 TGAGACAGCT CATGGAGGGT CTGGATTATT GTCATAAGAA GAACTTTTTG CATAGAGATA 
     3541 TTAAATGTTC CAATATCCTT CTAAATAATA GAGGGCAGAT AAAACTTGCA GACTTTGGAC 
     3601 TTGCTCGATT GTATAGCTCA GAAGAAAGTC GGCCGTATAC TAACAAGGTA ATTACTTTAT 
     3661 GGTACCGTCC ACCTGAACTG CTACTGGGAG AAGAACGATA CACACCAGCC ATTGATGTAT 
     3721 GGAGCTGTGG CTGTATCCTT GGCGAACTCT TCACTAAAAA ACCTATATTT CAAGCAAATC 
     3781 AGGAACTTGC ACAACTAGAA TTAATAAGCC GAATATGTGG GAGTCCATGT CCTGCAGTGT 
     3841 GGCCTGATGT AATCAAACTA CCATATTTCA ACACCATGAA ACCAAAGAAG CAATATCGTC 
     3901 GAAAGTTAAG AGAAGAATTT GTTTTTATTC CTGCAGCTGC GCTAGACTTA TTTGATTACA 
     3961 TGCTTGCCTT GGATCCTAGT AAGCGCTGCA CTGCTGAACA GGCTCTTCAG TGCGAGTTCC 
     4021 TCCGAGATGT GGAACCCTCA AAAATGCCTC CACCAGATCT CCCTTTATGG CAAGATTGTC 
     4081 ATGAGTTATG GAGTAAAAAA CGAAGAAGAC AGAAGCAGAT GGGCATGACT GATGATGTTT 
     4141 CCACAATTAA AGCCCCCAGG AAGGACTTGT CTCTGGGCTT GGATGACAGC AGAACCAACA 
     4201 CACCCCAGGG TGTGCTGCCA TCTTCACAGC TGAAATCTCA GGGCAGCTCA AATGTGGCAC 
     4261 CTGTAAAAAC AGGCCCTGGA CAGCACTTAA ACCACAGTGA ATTGGCAATT CTACTAAACC 
     4321 TACTACAATC TAAAACAAGT GTTAATATGG CTGATTTTGT CCAAGTGTTG AACATTAAGG 
     4381 TAAACTCTGA GACTCAACAG CAGCTAAATA AAATAAACCT TCCTGCTGGA ATTTTGGCAA 
     4441 CAGGTGAAAA ACAGACAGAT CCATCAACAC CACAACAGGA GTCTTCGAAA CCGTTGGGAG 
     4501 GAATTCAGCC TTCTTCTCAG ACCATCCAGC CTAAAGTGGA GACTGATGCT GCCCAGGCGG 
     4561 CTGTGCAGAG TGCATTTGCA GTTCTGTTGA CTCAGTTAAT AAAGGCTCAG CAGTCAAAGC 
     4621 AGAAAGATGT GCTACTAGAA GAGAGGGAAA ATGGATCGGG ACATGAAGCG TCATTACAAC 
     4681 TCAGGCCACC TCCAGAACCT AGCACTCCGG TGTCGGGACA AGATGACCTC ATCCAGCATC 
     4741 AAGATATGAG GATCTTGGAG CTAACGCCAG AACCAGACCG GCCTCGAATT CTGCCTCCTG 
     4801 ACCAACGACC TCCCGAGCCT CCTGAACCAC CACCAGTCAC TGAGGAAGAT CTAGATTATC 
     4861 GGACAGAAAA CCAGCATGTA CCCACCACCA GTTCTTCATT AACTGACCCT CATGCCGGAG 
     4921 TGAAGGCAGC CCTGTTACAG CTGCTTGCTC AGCATCAGCC CCAGGATGAC CCCAAAAGAG 
     4981 AAGGTGGGAT TGATTATCAA GCAGGAGACA CTTACGTGTC CACTTCAGAC TACAAGGACA 
     5041 ACTTTGGATC CTCTTCTTTC TCTTCTGCTC CTTATGTTAG CAATGATGGT CTAGGAAGCA 
     5101 GTTCTGCTCC ACCACTAGAA CGACGTAGTT TCATTGGAAA TTCAGATATT CAGTCTTTGG 
     5161 ATAACTACAG TACTGCTTCA TCTCATTCTG GTGGTCCACC TCAGCCTTCT GCCTTTTCTG 
     5221 AGTCATTTCC CAGTTCAGTA GCTGGATATG GAGACATTTA CCTCAATGCT GGTCCCATGT 
     5281 TGTTTAGTGG AGACAAGGAC CATAGATTTG AATATAGCCA TGGTCCTATT GCAGTCCTGG 
     5341 CAAACAGCAG TGACCCTTCC ACGGGGCCAG AGAGTACTCA TCCTTTGCCA GCAAAGATGC 
     5401 ACAACTATAA CTATGGTGGT AACTTACAGG AAAATCCGAG TGGCCCCAGC CTCATGCATG 
     5461 GACAGACCTG GACTTCTCCT GCCCAAGGAC CTGGATATTC ACAAGGATAC AGGGGACATA 
     5521 TTAGCACATC AACTGGCAGA GGCAGAGGCA GAGGGTTACC ATACACGCGT ACGCGGCCGC 
     5581 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5641 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5701 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5761 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5821 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5881 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5941 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6001 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6061 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6121 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6181 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6241 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6301 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6361 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6421 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6481 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6541 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6601 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6661 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6721 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6781 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6841 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6901 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6961 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7021 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7081 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7141 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7201 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7261 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7321 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7381 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7441 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7501 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7561 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7621 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7681 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7741 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7801 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7861 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7921 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7981 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8041 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8101 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8161 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8221 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8281 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8341 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8401 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8461 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8521 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8581 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8641 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8761 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8821 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8941 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9001 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9061 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9121 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9181 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9241 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9301 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9361 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//