LOCUS       RC217061                8589 bp    DNA     circular  SYN 
DEFINITION  TBC1D9B (Myc-DDK-tagged)-Human TBC1 domain family, member 9B (with GRAM domain) (TBC1D9B), transcript variant 2
ACCESSION   NM_015043
VERSION     NM_015043.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4744..4835 
                     /label="Myc-DDK"  
     misc_feature    4865..5450 
                     /label="PolyA signal"  
     misc_feature    4922..4941 
                     /label="Restriction Enzyme"  
     misc_feature    5726..6398 
                     /label="ColE1"  
     misc_feature    6828..7622 
                     /label="Kan/Neo"  
     misc_feature    7657..7959 
                     /label="SV40 ori"  
     misc_feature    8045..8522 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4726 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="TBC1D9B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCTGAGC CCGGAGGAGG TGCTGGTGGC CAATGCGCTG TGGGTGACGG 
     1081 AGCGGGCCAA CCCCTTCTTC GTGCTGCAGC GACGCCGGGG CCACGGCAGG GGCGGCGGCC 
     1141 TTACGGGTCT TCTCGTGGGC ACCCTGGACG TGGTGCTGGA CTCCAGTGCC CGCGTGGCCC 
     1201 CTTACCGCAT CCTGCACCAG ACCCAGGACT CCCAGGTCTA CTGGACAGTG GCGTGTGGTT 
     1261 CTTCCCGCAA AGAGATCACA AAACACTGGG AATGGCTGGA AAATAACTTG CTCCAGACAC 
     1321 TGTCCATCTT CGACAGTGAG GAAGATATCA CCACCTTCGT CAAGGGCAAG ATACACGGAA 
     1381 TCATCGCAGA AGAGAACAAG AACCTGCAGC CCCAGGGAGA CGAGGACCCC GGGAAGTTCA 
     1441 AGGAGGCTGA GCTGAAGATG CGGAAGCAGT TTGGGATGCC TGAGGGCGAG AAGCTGGTGA 
     1501 ATTACTACTC CTGCAGCTAC TGGAAGGGCC GCGTGCCCCG GCAGGGCTGG CTGTACCTGA 
     1561 CGGTCAACCA CCTGTGCTTC TACTCCTTCC TGCTGGGGAA GGAAGTGAGC CTCGTGGTGC 
     1621 AGTGGGTGGA CATAACGCGT CTGGAGAAGA ACGCCACCCT GCTCTTCCCC GAGAGCATCC 
     1681 GTGTGGACAC CCGCGACCAG GAGCTCTTCT TCTCCATGTT CCTCAACATC GGCGAGACCT 
     1741 TCAAGCTCAT GGAGCAGTTG GCCAACCTGG CCATGCGGCA GCTGCTGGAC AGCGAGGGCT 
     1801 TCCTGGAGGA CAAGGCCCTG CCTAGGCCCA TCCGGCCACA CAGGAACATC TCAGCCCTGA 
     1861 AGCGAGACCT GGACGCCCGA GCCAAGAATG AGTGCTACCG AGCCACGTTC CGGCTGCCCA 
     1921 GGGATGAGCG GCTAGACGGC CACACAAGCT GCACCCTGTG GACGCCGTTC AACAAGCTGC 
     1981 ACATCCCTGG CCAGATGTTC ATCTCCAACA ACTACATCTG CTTCGCCAGC AAGGAGGAGG 
     2041 ACGCTTGCCA CCTCATCATA CCCCTGAGGG AGGTGACCAT TGTCGAAAAA GCTGACAGCT 
     2101 CCAGCGTGCT GCCCAGCCCC CTGTCCATCA GCACCAAAAG CAAAATGACA TTCCTGTTTG 
     2161 CCAACCTGAA AGACCGTGAT TTCTTGGTGC AGAGGATCTC TGACTTCCTC CAGAAAACAC 
     2221 CATCCAAGCA GCCAGGCAGT ATCGGGAGCA GGAAAGCCAG TGTTGTGGAC CCTAGCACAG 
     2281 AGTCTTCCCC AGCTCCTCAG GAGGGGTCGG AGCAGCCCGC CAGCCCAGCC TCTCCCCTCA 
     2341 GCAGCCGCCA GAGCTTCTGT GCGCAGGAGG CGCCAACCGC ATCCCAGGGC CTGCTGAAGC 
     2401 TCTTCCAGAA AAACTCGCCC ATGGAGGACC TTGGAGCCAA GGGGGCCAAG GAGAAGATGA 
     2461 AAGAGGAGTC ATGGCACATC CACTTCTTCG AGTACGGGCG TGGCGTGTGC ATGTACCGCA 
     2521 CAGCCAAGAC GCGGGCACTG GTCCTGAAGG GTATCCCTGA GAGCCTCCGG GGAGAGCTGT 
     2581 GGCTCCTCTT CTCCGGGGCC TGGAATGAGA TGGTGACTCA CCCCGGGTAC TATGCTGAGC 
     2641 TGGTGGAGAA GTCCACCGGG AAGTACAGCC TGGCCACAGA GGAGATCGAG CGAGACCTGC 
     2701 ACCGCTCCAT GCCCGAGCAC CCTGCCTTCC AGAACGAGCT GGGGATTGCT GCCCTCCGGC 
     2761 GGGTGCTGAC TGCCTATGCC TTCCGAAACC CCACCATCGG CTACTGCCAG GCAATGAACA 
     2821 TCGTGACCTC GGTGCTCCTG CTCTATGGCA GTGAGGAGGA GGCCTTCTGG CTCCTGGTGG 
     2881 CCCTGTGCGA GCGCATGCTG CCCGACTACT ACAACACCAG GGTGGTGGGA GCCCTGGTGG 
     2941 ACCAAGGCAT CTTCGAAGAG CTCACGAGAG ACTTCCTGCC GCAGCTCTCG GAGAAGATGC 
     3001 AGGACCTGGG GGTGATCTCC AGCATCTCGC TGTCCTGGTT CCTGACCCTC TTCCTCAGCG 
     3061 TCATGCCCTT CGAGAGCGCC GTGGTCATCG TCGACTGCTT TTTCTATGAG GGCATCAAGG 
     3121 TGATCCTGCA GGTGGCCCTG GCCGTCCTGG ACGCCAACAT GGAGCAGCTG CTGGGCTGCA 
     3181 GCGACGAGGG CGAGGCCATG ACCATGCTGG GCAGATACCT GGATAATGTG GTCAACAAGC 
     3241 AGAGTGTCTC TCCTCCTATC CCGCACCTCC GTGCCTTGCT GAGCAGCAGC GATGACCCCC 
     3301 CTGCAGAGGT GGACATCTTT GAGCTCCTGA AAGTGTCCTA TGAGAAATTC AGCAGCCTGA 
     3361 GGGCCGAAGA CATTGAGCAG ATGCGGTTTA AACAGAGGCT GAAAGTGATC CAGTCCTTGG 
     3421 AGGACACGGC CAAGAGGAGT GTGGTCCGAG CTATACCTGT GGACATTGGT TTCTCCATTG 
     3481 AAGAGCTGGA GGACCTTTAC ATGGTGTTTA AGGCCAAGCA CCTGGCTAGC CAGTACTGGG 
     3541 GGTGCAGCCG CACAATGGCC GGCCGTCGGG ACCCCAGCCT GCCCTACCTG GAGCAGTACC 
     3601 GGATTGATGC CAGCCAGTTC CGGGAACTCT TTGCCAGCCT GACACCCTGG GCCTGTGGCT 
     3661 CCCACACACC TCTGCTGGCA GGGCGCATGT TCAGGCTCCT GGACGAAAAC AAGGACTCGC 
     3721 TGATCAACTT CAAGGAGTTC GTGACAGGGA TGAGCGGGAT GTACCACGGG GACCTGACAG 
     3781 AGAAGCTCAA GGTGCTCTAC AAGCTACACC TTCCCCCAGC TCTGAGCCCA GAGGAAGCCG 
     3841 AGTCAGCCCT GGAGGCGGCC CATTATTTCA CAGAGGACAG CTCCTCAGAA GAAGCACTAC 
     3901 CACAGGAAGA GCAAGAAGGA AGTGGAAGTG AGGAGAGAGG AGAGGAGAAG GGGACCAGCT 
     3961 CTCCGGACTA TCGGCACTAC CTTCGAATGT GGGCCAAGGA GAAAGAGGCT CAGAAGGAGA 
     4021 CGATTAAGGA TCTTCCCAAG ATGAACCAGG AGCAGTTCAT TGAGCTGTGC AAGACGCTTT 
     4081 ACAACATGTT CAGTGAAGAC CCCATGGAGC AGGACCTGTA CCACGCCATC GCCACCGTGG 
     4141 CCAGCCTCCT GCTCCGCATC GGAGAGGTGG GGAAGAAGTT CTCAGCCCGC ACAGGCAGGA 
     4201 AGCCCAGGGA CTGTGCCACT GAGGAGGACG AGCCACCAGC ACCCGAACTG CATCAGGACG 
     4261 CAGCCAGGGA GCTTCAGCCC CCAGCTGCAG GAGACCCCCA AGCCAAAGCA GGCGGAGACA 
     4321 CACACCTCGG AACAGCCCCA CAGGAGAGCC AGGTGGTGGT GGAGGGGGGC AGCGGCGAGG 
     4381 GACAGGGCTC ACCCTCCCAG CTGCTGTCTG ACGATGAAAC CAAAGACGAC ATGTCCATGT 
     4441 CCTCCTACTC GGTGGTCAGC ACGGGCTCCC TGCAATGTGA AGACCTTGCA GACGACACGG 
     4501 TGCTGGTGGG CGGGGAGGCC TGCAGCCCCA CAGCGCGCAT CGGCGGCACC GTCGACACCG 
     4561 ACTGGTGCAT CTCCTTTGAG CAGATCCTGG CCTCCATCCT GACGGAGTCC GTGCTGGTGA 
     4621 ACTTCTTTGA GAAGAGAGTG GACATTGGAC TCAAGATCAA GGACCAAAAG AAAGTGGAGA 
     4681 GACAGTTCAG CACCGCCAGT GACCATGAGC AGCCTGGAGT TTCCGGCAGC GGACCGACGC 
     4741 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4801 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4861 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4921 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4981 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5041 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5101 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5161 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5221 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5281 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5341 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5401 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5461 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5521 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5581 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5641 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5701 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5761 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5821 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5881 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5941 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6001 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6061 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6121 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6181 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6241 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6301 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6361 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6421 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6481 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6541 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6601 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6661 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6721 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6781 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6841 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6901 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6961 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7021 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7081 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7141 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7201 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7261 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7321 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7381 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7441 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7501 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7561 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7621 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7681 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7741 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7801 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7921 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7981 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8161 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8221 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8281 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8341 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8401 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8461 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8521 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8581 GCGAATTTT
//