LOCUS       RC217055                7782 bp    DNA     circular  SYN 
DEFINITION  OPA1 (Myc-DDK-tagged)-Human optic atrophy 1 (autosomal dominant) (OPA1), nuclear gene encoding mitochondrial protein, transcript variant 4
ACCESSION   NM_130833
VERSION     NM_130833.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3937..4028 
                     /label="Myc-DDK"  
     misc_feature    4058..4643 
                     /label="PolyA signal"  
     misc_feature    4115..4134 
                     /label="Restriction Enzyme"  
     misc_feature    4919..5591 
                     /label="ColE1"  
     misc_feature    6021..6815 
                     /label="Kan/Neo"  
     misc_feature    6850..7152 
                     /label="SV40 ori"  
     misc_feature    7238..7715 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3912..3919 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3912 
                     /label="OPA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGACTA CGTCGGGCCG CTGTGGCCTG TGAGGTCTGC CAGTCTTTAG 
     1081 TGAAACACAG CTCTGGAATA AAAGGAAGTT TACCACTACA AAAACTACAT CTGGTTTCAC 
     1141 GAAGCATTTA TCATTCACAT CATCCTACCT TAAAGCTTCA ACGACCCCAA TTAAGGACAT 
     1201 CCTTTCAGCA GTTCTCTTCT CTGACAAACC TTCCTTTACG TAAACTGAAA TTCTCTCCAA 
     1261 TTAAATATGG CTACCAGCCT CGCAGGAATT TTTGGCCAGC AAGATTAGCT ACGAGACTCT 
     1321 TAAAACTTCG CTATCTCATA CTAGGATCGG CTGTTGGGGG TGGCTACACA GCCAAAAAGA 
     1381 CTTTTGATCA GTGGAAAGAT ATGATACCGG ACCTTAGTGA ATATAAATGG ATTGTGCCTG 
     1441 ACATTGTGTG GGAAATTGAT GAGTATATCG ATTTTGGTTC TCCGGAAGAA ACGGCGTTTA 
     1501 GAGCAACAGA TCGTGGATCT GAAAGTGACA AGCATTTTAG AAAGGGTCTG CTTGGTGAGC 
     1561 TCATTCTCTT ACAACAACAA ATTCAAGAGC ATGAAGAGGA AGCGCGCAGA GCCGCTGGCC 
     1621 AATATAGCAC GAGCTATGCC CAACAGAAGC GCAAGGTGTC AGACAAAGAG AAAATTGACC 
     1681 AACTTCAGGA AGAACTTCTG CACACTCAGT TGAAGTATCA GAGAATCTTG GAACGATTAG 
     1741 AAAAGGAGAA CAAAGAATTG AGAAAATTAG TATTGCAGAA AGATGACAAA GGCATTCATC 
     1801 ATAGAAAGCT TAAGAAATCT TTGATTGACA TGTATTCTGA AGTTCTTGAT GTTCTCTCTG 
     1861 ATTATGATGC CAGTTATAAT ACGCAAGATC ATCTGCCACG GGTTGTTGTG GTTGGAGATC 
     1921 AGAGTGCTGG AAAGACTAGT GTGTTGGAAA TGATTGCCCA AGCTCGAATA TTCCCAAGAG 
     1981 GATCTGGGGA GATGATGACA CGTTCTCCAG TTAAGGTGAC TCTGAGTGAA GGTCCTCACC 
     2041 ATGTGGCCCT ATTTAAAGAT AGTTCTCGGG AGTTTGATCT TACCAAAGAA GAAGATCTTG 
     2101 CAGCATTAAG ACATGAAATA GAACTTCGAA TGAGGAAAAA TGTGAAAGAA GGCTGTACCG 
     2161 TTAGCCCTGA GACCATATCC TTAAATGTAA AAGGCCCTGG ACTACAGAGG ATGGTGCTTG 
     2221 TTGACTTACC AGGTGTGATT AATACTGTGA CATCAGGCAT GGCTCCTGAC ACAAAGGAAA 
     2281 CTATTTTCAG TATCAGCAAA GCTTACATGC AGAATCCTAA TGCCATCATA CTGTGTATTC 
     2341 AAGATGGATC TGTGGATGCT GAACGCAGTA TTGTTACAGA CTTGGTCAGT CAAATGGACC 
     2401 CTCATGGAAG GAGAACCATA TTCGTTTTGA CCAAAGTAGA CCTGGCAGAG AAAAATGTAG 
     2461 CCAGTCCAAG CAGGATTCAG CAGATAATTG AAGGAAAGCT CTTCCCAATG AAAGCTTTAG 
     2521 GTTATTTTGC TGTTGTAACA GGAAAAGGGA ACAGCTCTGA AAGCATTGAA GCTATAAGAG 
     2581 AATATGAAGA AGAGTTTTTT CAGAATTCAA AGCTCCTAAA GACAAGCATG CTAAAGGCAC 
     2641 ACCAAGTGAC TACAAGAAAT TTAAGCCTTG CAGTATCAGA CTGCTTTTGG AAAATGGTAC 
     2701 GAGAGTCTGT TGAACAACAG GCTGATAGTT TCAAAGCAAC ACGTTTTAAC CTTGAAACTG 
     2761 AATGGAAGAA TAACTATCCT CGCCTGCGGG AACTTGACCG GAATGAACTA TTTGAAAAAG 
     2821 CTAAAAATGA AATCCTTGAT GAAGTTATCA GTCTGAGCCA GGTTACACCA AAACATTGGG 
     2881 AGGAAATCCT TCAACAATCT TTGTGGGAAA GAGTATCAAC TCATGTGATT GAAAACATCT 
     2941 ACCTTCCAGC TGCGCAGACC ATGAATTCAG GAACTTTTAA CACCACAGTG GATATCAAGC 
     3001 TTAAACAGTG GACTGATAAA CAACTTCCTA ATAAAGCAGT AGAGGTTGCT TGGGAGACCC 
     3061 TACAAGAAGA ATTTTCCCGC TTTATGACAG AACCGAAAGG GAAAGAGCAT GATGACATAT 
     3121 TTGATAAACT TAAAGAGGCT GTTAAGGAAG AAAGTATTAA ACGACACAAG TGGAATGACT 
     3181 TTGCGGAGGA CAGCTTGAGG GTTATTCAAC ACAATGCTTT GGAAGACCGA TCCATATCTG 
     3241 ATAAACAGCA ATGGGATGCA GCTATTTATT TTATGGAAGA GGCTCTGCAG GCTCGTCTCA 
     3301 AGGATACTGA AAATGCAATT GAAAACATGG TGGGTCCAGA CTGGAAAAAG AGGTGGTTAT 
     3361 ACTGGAAGAA TCGGACCCAA GAACAGTGTG TTCACAATGA AACCAAGAAT GAATTGGAGA 
     3421 AGATGTTGAA ATGTAATGAG GAGCACCCAG CTTATCTTGC AAGTGATGAA ATAACCACAG 
     3481 TCCGGAAGAA CCTTGAATCC CGAGGAGTAG AAGTAGATCC AAGCTTGATT AAGGATACTT 
     3541 GGCATCAAGT TTATAGAAGA CATTTTTTAA AAACAGCTCT AAACCATTGT AACCTTTGTC 
     3601 GAAGAGGTTT TTATTACTAC CAAAGGCATT TTGTAGATTC TGAGTTGGAA TGCAATGATG 
     3661 TGGTCTTGTT TTGGCGTATA CAGCGCATGC TTGCTATCAC CGCAAATACT TTAAGGCAAC 
     3721 AACTTACAAA TACTGAAGTT AGGCGATTAG AGAAAAATGT TAAAGAGGTA TTGGAAGATT 
     3781 TTGCTGAAGA TGGTGAGAAG AAGATTAAAT TGCTTACTGG TAAACGCGTT CAACTGGCGG 
     3841 AAGACCTCAA GAAAGTTAGA GAAATTCAAG AAAAACTTGA TGCTTTCATT GAAGCTCTTC 
     3901 ATCAGGAGAA AAGCGGACCG AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3961 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4021 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4081 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4141 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4201 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4261 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4321 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4381 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4441 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4501 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4561 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4621 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4681 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4741 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4801 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4861 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4921 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4981 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5041 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5101 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5161 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5221 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5281 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5341 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5401 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5461 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5521 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5581 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5641 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5701 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5761 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5821 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5881 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5941 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6001 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6061 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6121 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6181 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6241 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6301 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6361 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6421 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6481 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6541 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6601 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6661 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6721 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6781 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6841 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6901 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6961 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7021 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7081 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7141 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7201 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7261 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7321 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7381 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7441 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7501 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7561 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7621 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7681 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7741 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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