LOCUS       RC217046                9024 bp    DNA     circular  SYN 
DEFINITION  PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1a
ACCESSION   NM_001083602
VERSION     NM_001083602.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5181..5272 
                     /label="Myc-DDK"  
     misc_feature    5302..5887 
                     /label="PolyA signal"  
     misc_feature    5359..5378 
                     /label="Restriction Enzyme"  
     misc_feature    6163..6835 
                     /label="ColE1"  
     misc_feature    7265..8059 
                     /label="Kan/Neo"  
     misc_feature    8094..8396 
                     /label="SV40 ori"  
     misc_feature    8482..8959 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5163..5169 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5163 
                     /label="PTCH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA GCGAAGTTTC 
     1081 AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG TTCTTGGTTG 
     1141 TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC CTCGAGACCA 
     1201 ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA AATTATACTC 
     1261 GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG ACCCCTAAAG 
     1321 AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC TCGGCACTCC 
     1381 AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA CATTTGTGTT 
     1441 ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA GAATATCTTT 
     1501 ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA TTACAGTCTG 
     1561 GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC CCTTTGGAAT 
     1621 TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA ATGCTGAATA 
     1681 AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC GATCCAGACT 
     1741 GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC CTTGTTTTGA 
     1801 ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG TTGATTGTGG 
     1861 GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG CAGACCATGT 
     1921 TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG TATGTCTCAC 
     1981 ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG AGGACATATG 
     2041 TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT TCCTTCACCA 
     2101 CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC CGCGTGGCCA 
     2161 GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG GACTGCTCCA 
     2221 AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA GTGGCTGCAG 
     2281 GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT CAGGTTTTGC 
     2341 CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC GCCTTCAGTG 
     2401 AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC CTGAAGCGCA 
     2461 CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC ATGGCCGCGT 
     2521 TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA GTGGTGTTCA 
     2581 ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA TATCGACGCG 
     2641 AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC AGAGTGATTC 
     2701 AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC CCCCCACCTC 
     2761 CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC ACTGTCCAGC 
     2821 TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG CCGCGCTCCG 
     2881 AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG AGCCCAGAGA 
     2941 GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC CACTGCCTCG 
     3001 AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT GCTCCTTTCC 
     3061 TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG CTGGGGGTCA 
     3121 GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT GTACCTCGGG 
     3181 AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC TACAACATGT 
     3241 ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC GACCTACACA 
     3301 GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT CCCAAAATGT 
     3361 GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC AGTGACTGGG 
     3421 AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA GTCCTTGCCT 
     3481 ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC CAGTTGACTA 
     3541 AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC ATCTACCTGA 
     3601 CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC ATCCGGCCAC 
     3661 ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG CTGAGAATCC 
     3721 CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC TTGCGGGACA 
     3781 CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC TATACGAGCC 
     3841 TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG TACATCGGCC 
     3901 TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC CTCGTGTGCG 
     3961 CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG GCGCTGATGA 
     4021 CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC GTGCCCGTGG 
     4081 TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT GCTTTGGCCT 
     4141 TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG CACATGTTTG 
     4201 CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG GCGGGATCTG 
     4261 AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC ATCCTCGGCG 
     4321 TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA TATCCTGAGG 
     4381 TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA CCCCCCAGCG 
     4441 TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC TCCGACTCGG 
     4501 AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC TACGAGGCCC 
     4561 AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA AACCCCGTCT 
     4621 TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG AACCCGAGAC 
     4681 AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG CAGCCCCGCA 
     4741 GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA GACGCTTTTG 
     4801 AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC CCTCGCGGGG 
     4861 CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC GTGCCCGGCT 
     4921 ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC GTGCACCCGC 
     4981 CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC TACCCTGAGA 
     5041 CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT GAGAGGAGGG 
     5101 ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG CCCCGGGGAA 
     5161 GCAGCTCCAA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5221 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5281 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5341 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5401 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5461 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5521 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5581 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5641 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5701 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5761 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5821 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5881 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5941 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6001 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6061 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6121 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6181 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6241 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6301 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6361 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6421 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6481 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6541 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6601 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6661 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6721 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6781 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6841 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6901 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6961 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7081 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7141 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7201 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7261 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7321 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7381 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7441 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7501 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7561 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7621 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7681 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7741 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7801 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7861 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7921 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7981 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8041 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8101 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8161 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8221 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8281 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8341 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8401 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8461 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8521 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8581 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8641 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8701 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8761 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8821 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8881 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8941 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9001 TAACAAAAAT TTAACGCGAA TTTT
//