LOCUS       RC217035L1                9144 bp    DNA     circular  SYN 
DEFINITION  NRP1 (Myc-DDK-tagged)-Human neuropilin 1 (NRP1), transcript variant 1
ACCESSION   NM_003873
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7435..7615 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8343..9002 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7774..8342 
                     /label="ColE1"  
     misc_feature    6310..6330 
                     /label="LR50 primer"  
     misc_feature    6107..6178 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6319..6910 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6089..6095 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6089 
                     /label="NRP1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGAGGGGG CTGCCGCTCC TCTGCGCCGT 
     3361 GCTCGCCCTC GTCCTCGCCC CGGCCGGCGC TTTTCGCAAC GATAAATGTG GCGATACTAT 
     3421 AAAAATTGAA AGCCCCGGGT ACCTTACATC TCCTGGTTAT CCTCATTCTT ATCACCCAAG 
     3481 TGAAAAATGC GAATGGCTGA TTCAGGCTCC GGACCCATAC CAGAGAATTA TGATCAACTT 
     3541 CAACCCTCAC TTCGATTTGG AGGACAGAGA CTGCAAGTAT GACTACGTGG AAGTCTTCGA 
     3601 TGGAGAAAAT GAAAATGGAC ATTTTAGGGG AAAGTTCTGT GGAAAGATAG CCCCTCCTCC 
     3661 TGTTGTGTCT TCAGGGCCAT TTCTTTTTAT CAAATTTGTC TCTGACTACG AAACACATGG 
     3721 TGCAGGATTT TCCATACGTT ATGAACTTTT CAAGAGAGGT CCTGAATGTT CCCAGAACTA 
     3781 CACAACACCT AGTGGAGTGA TAAAGTCCCC CGGATTCCCT GAAAAATATC CCAACAGCCT 
     3841 TGAATGCACT TATATTGTCT TTGCGCCAAA GATGTCAGAG ATTATCCTGG AATTTGAAAG 
     3901 CTTTGACCTG GAGCCTGACT CAAATCCTCC AGGGGGGATG TTCTGTCGCT ACGACCGGCT 
     3961 AGAAATCTGG GATGGATTCC CTGATGTTGG CCCTCACATT GGGCGTTACT GTGGACAGAA 
     4021 AACACCAGGT CGAATCCGAT CCTCATCGGG CATTCTCTCC ATGGTTTTTT ACACCGACAG 
     4081 CGCGATAGCA AAAGAAGGTT TCTCAGCAAA CTACAGTGTC TTGCAGAGCA GTGTCTCAGA 
     4141 AGATTTCAAA TGTATGGAAG CTCTGGGCAT GGAATCAGGA GAAATTCATT CTGACCAGAT 
     4201 CACAGCTTCT TCCCAGTATA GCACCAACTG GTCTGCAGAG CGCTCCCGCC TGAACTACCC 
     4261 TGAGAATGGG TGGACTCCCG GAGAGGATTC CTACCGAGAG TGGATACAGG TAGACTTGGG 
     4321 CCTTCTGCGC TTTGTCACGG CTGTCGGGAC ACAGGGCGCC ATTTCAAAAG AAACCAAGAA 
     4381 GAAATATTAT GTCAAGACTT ACAAGATCGA CGTTAGCTCC AACGGGGAAG ACTGGATCAC 
     4441 CATAAAAGAA GGAAACAAAC CTGTTCTCTT TCAGGGAAAC ACCAACCCTA CAGATGTTGT 
     4501 GGTTGCAGTA TTCCCCAAAC CACTGATAAC TCGATTTGTC CGAATCAAGC CTGCAACTTG 
     4561 GGAAACTGGC ATATCTATGA GATTTGAAGT ATATGGTTGC AAGATAACAG ATTATCCTTG 
     4621 CTCTGGAATG TTGGGTATGG TGTCTGGACT TATTTCTGAC TCCCAGATCA CATCATCCAA 
     4681 CCAAGGGGAC AGAAACTGGA TGCCTGAAAA CATCCGCCTG GTAACCAGTC GCTCTGGCTG 
     4741 GGCACTTCCA CCCGCACCTC ATTCCTACAT CAATGAGTGG CTCCAAATAG ACCTGGGGGA 
     4801 GGAGAAGATC GTGAGGGGCA TCATCATTCA GGGTGGGAAG CACCGAGAGA ACAAGGTGTT 
     4861 CATGAGGAAG TTCAAGATCG GGTACAGCAA CAACGGCTCG GACTGGAAGA TGATCATGGA 
     4921 TGACAGCAAA CGCAAGGCGA AGTCTTTTGA GGGCAACAAC AACTATGATA CACCTGAGCT 
     4981 GCGGACTTTT CCAGCTCTCT CCACGCGATT CATCAGGATC TACCCCGAGA GAGCCACTCA 
     5041 TGGCGGACTG GGGCTCAGAA TGGAGCTGCT GGGCTGTGAA GTGGAAGCCC CTACAGCTGG 
     5101 ACCGACCACT CCCAACGGGA ACTTGGTGGA TGAATGTGAT GACGACCAGG CCAACTGCCA 
     5161 CAGTGGAACA GGTGATGACT TCCAGCTCAC AGGTGGCACC ACTGTGCTGG CCACAGAAAA 
     5221 GCCCACGGTC ATAGACAGCA CCATACAATC AGAGTTTCCA ACATATGGTT TTAACTGTGA 
     5281 ATTTGGCTGG GGCTCTCACA AGACCTTCTG CCACTGGGAA CATGACAATC ACGTGCAGCT 
     5341 CAAGTGGAGT GTGTTGACCA GCAAGACGGG ACCCATTCAG GATCACACAG GAGATGGCAA 
     5401 CTTCATCTAT TCCCAAGCTG ACGAAAATCA GAAGGGCAAA GTGGCTCGCC TGGTGAGCCC 
     5461 TGTGGTTTAT TCCCAGAACT CTGCCCACTG CATGACCTTC TGGTATCACA TGTCTGGGTC 
     5521 CCACGTCGGC ACACTCAGGG TCAAACTGCG CTACCAGAAG CCAGAGGAGT ACGATCAGCT 
     5581 GGTCTGGATG GCCATTGGAC ACCAAGGTGA CCACTGGAAG GAAGGGCGTG TCTTGCTCCA 
     5641 CAAGTCTCTG AAACTTTATC AGGTGATTTT CGAGGGCGAA ATCGGAAAAG GAAACCTTGG 
     5701 TGGGATTGCT GTGGATGACA TTAGTATTAA TAACCACATT TCACAAGAAG ATTGTGCAAA 
     5761 ACCAGCAGAC CTGGATAAAA AGAACCCAGA AATTAAAATT GATGAAACAG GGAGCACGCC 
     5821 AGGATACGAA GGTGAAGGAG AAGGTGACAA GAACATCTCC AGGAAGCCAG GCAATGTGTT 
     5881 GAAGACCTTA GACCCCATCC TCATCACCAT CATAGCCATG AGCGCCCTGG GGGTCCTCCT 
     5941 GGGGGCTGTC TGTGGGGTCG TGCTGTACTG TGCCTGTTGG CATAATGGGA TGTCAGAAAG 
     6001 AAACTTGTCT GCCCTGGAGA ACTATAACTT TGAACTTGTG GATGGTGTGA AGTTGAAAAA 
     6061 AGACAAACTG AATACACAGA GTACTTATTC GGAGGCAACG CGTACGCGGC CGCTCGAGCA 
     6121 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     6181 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6241 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6301 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6361 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6421 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6481 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6541 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6601 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6661 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6721 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6781 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6841 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     6901 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     6961 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7021 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7081 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7141 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7201 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7261 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7321 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7381 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7441 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7501 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7561 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7621 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7681 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7741 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7801 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7861 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     7921 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     7981 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8041 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8101 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8161 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8221 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8281 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8341 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8401 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8461 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8521 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8581 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8641 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8701 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8761 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8821 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     8881 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     8941 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9001 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9061 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     9121 TTTCCCCGAA AAGTGCCACC TGAC
//