LOCUS       RC216999                9222 bp    DNA     circular  SYN 
DEFINITION  PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b
ACCESSION   NM_000264
VERSION     NM_000264.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5379..5470 
                     /label="Myc-DDK"  
     misc_feature    5500..6085 
                     /label="PolyA signal"  
     misc_feature    5557..5576 
                     /label="Restriction Enzyme"  
     misc_feature    6361..7033 
                     /label="ColE1"  
     misc_feature    7463..8257 
                     /label="Kan/Neo"  
     misc_feature    8292..8594 
                     /label="SV40 ori"  
     misc_feature    8680..9157 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5361..5367 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5361 
                     /label="PTCH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGCT GGTAACGCCG CCGAGCCCCA GGACCGCGGC GGCGGCGGCA 
     1081 GCGGCTGTAT CGGTGCCCCG GGACGGCCGG CTGGAGGCGG GAGGCGCAGA CGGACGGGGG 
     1141 GGCTGCGCCG TGCTGCCGCG CCGGACCGGG ACTATCTGCA CCGGCCCAGC TACTGCGACG 
     1201 CCGCCTTCGC TCTGGAGCAG ATTTCCAAGG GGAAGGCTAC TGGCCGGAAA GCGCCGCTGT 
     1261 GGCTGAGAGC GAAGTTTCAG AGACTCTTAT TTAAACTGGG TTGTTACATT CAAAAAAACT 
     1321 GCGGCAAGTT CTTGGTTGTG GGCCTCCTCA TATTTGGGGC CTTCGCGGTG GGATTAAAAG 
     1381 CAGCGAACCT CGAGACCAAC GTGGAGGAGC TGTGGGTGGA AGTTGGAGGA CGAGTAAGTC 
     1441 GTGAATTAAA TTATACTCGC CAGAAGATTG GAGAAGAGGC TATGTTTAAT CCTCAACTCA 
     1501 TGATACAGAC CCCTAAAGAA GAAGGTGCTA ATGTCCTGAC CACAGAAGCG CTCCTACAAC 
     1561 ACCTGGACTC GGCACTCCAG GCCAGCCGTG TCCATGTATA CATGTACAAC AGGCAGTGGA 
     1621 AATTGGAACA TTTGTGTTAC AAATCAGGAG AGCTTATCAC AGAAACAGGT TACATGGATC 
     1681 AGATAATAGA ATATCTTTAC CCTTGTTTGA TTATTACACC TTTGGACTGC TTCTGGGAAG 
     1741 GGGCGAAATT ACAGTCTGGG ACAGCATACC TCCTAGGTAA ACCTCCTTTG CGGTGGACAA 
     1801 ACTTCGACCC TTTGGAATTC CTGGAAGAGT TAAAGAAAAT AAACTATCAA GTGGACAGCT 
     1861 GGGAGGAAAT GCTGAATAAG GCTGAGGTTG GTCATGGTTA CATGGACCGC CCCTGCCTCA 
     1921 ATCCGGCCGA TCCAGACTGC CCCGCCACAG CCCCCAACAA AAATTCAACC AAACCTCTTG 
     1981 ATATGGCCCT TGTTTTGAAT GGTGGATGTC ATGGCTTATC CAGAAAGTAT ATGCACTGGC 
     2041 AGGAGGAGTT GATTGTGGGT GGCACAGTCA AGAACAGCAC TGGAAAACTC GTCAGCGCCC 
     2101 ATGCCCTGCA GACCATGTTC CAGTTAATGA CTCCCAAGCA AATGTACGAG CACTTCAAGG 
     2161 GGTACGAGTA TGTCTCACAC ATCAACTGGA ACGAGGACAA AGCGGCAGCC ATCCTGGAGG 
     2221 CCTGGCAGAG GACATATGTG GAGGTGGTTC ATCAGAGTGT CGCACAGAAC TCCACTCAAA 
     2281 AGGTGCTTTC CTTCACCACC ACGACCCTGG ACGACATCCT GAAATCCTTC TCTGACGTCA 
     2341 GTGTCATCCG CGTGGCCAGC GGCTACTTAC TCATGCTCGC CTATGCCTGT CTAACCATGC 
     2401 TGCGCTGGGA CTGCTCCAAG TCCCAGGGTG CCGTGGGGCT GGCTGGCGTC CTGCTGGTTG 
     2461 CACTGTCAGT GGCTGCAGGA CTGGGCCTGT GCTCATTGAT CGGAATTTCC TTTAACGCTG 
     2521 CAACAACTCA GGTTTTGCCA TTTCTCGCTC TTGGTGTTGG TGTGGATGAT GTTTTTCTTC 
     2581 TGGCCCACGC CTTCAGTGAA ACAGGACAGA ATAAAAGAAT CCCTTTTGAG GACAGGACCG 
     2641 GGGAGTGCCT GAAGCGCACA GGAGCCAGCG TGGCCCTCAC GTCCATCAGC AATGTCACAG 
     2701 CCTTCTTCAT GGCCGCGTTA ATCCCAATTC CCGCTCTGCG GGCGTTCTCC CTCCAGGCAG 
     2761 CGGTAGTAGT GGTGTTCAAT TTTGCCATGG TTCTGCTCAT TTTTCCTGCA ATTCTCAGCA 
     2821 TGGATTTATA TCGACGCGAG GACAGGAGAC TGGATATTTT CTGCTGTTTT ACAAGCCCCT 
     2881 GCGTCAGCAG AGTGATTCAG GTTGAACCTC AGGCCTACAC CGACACACAC GACAATACCC 
     2941 GCTACAGCCC CCCACCTCCC TACAGCAGCC ACAGCTTTGC CCATGAAACG CAGATTACCA 
     3001 TGCAGTCCAC TGTCCAGCTC CGCACGGAGT ACGACCCCCA CACGCACGTG TACTACACCA 
     3061 CCGCTGAGCC GCGCTCCGAG ATCTCTGTGC AGCCCGTCAC CGTGACACAG GACACCCTCA 
     3121 GCTGCCAGAG CCCAGAGAGC ACCAGCTCCA CAAGGGACCT GCTCTCCCAG TTCTCCGACT 
     3181 CCAGCCTCCA CTGCCTCGAG CCCCCCTGTA CGAAGTGGAC ACTCTCATCT TTTGCTGAGA 
     3241 AGCACTATGC TCCTTTCCTC TTGAAACCAA AAGCCAAGGT AGTGGTGATC TTCCTTTTTC 
     3301 TGGGCTTGCT GGGGGTCAGC CTTTATGGCA CCACCCGAGT GAGAGACGGG CTGGACCTTA 
     3361 CGGACATTGT ACCTCGGGAA ACCAGAGAAT ATGACTTTAT TGCTGCACAA TTCAAATACT 
     3421 TTTCTTTCTA CAACATGTAT ATAGTCACCC AGAAAGCAGA CTACCCGAAT ATCCAGCACT 
     3481 TACTTTACGA CCTACACAGG AGTTTCAGTA ACGTGAAGTA TGTCATGTTG GAAGAAAACA 
     3541 AACAGCTTCC CAAAATGTGG CTGCACTACT TCAGAGACTG GCTTCAGGGA CTTCAGGATG 
     3601 CATTTGACAG TGACTGGGAA ACCGGGAAAA TCATGCCAAA CAATTACAAG AATGGATCAG 
     3661 ACGATGGAGT CCTTGCCTAC AAACTCCTGG TGCAAACCGG CAGCCGCGAT AAGCCCATCG 
     3721 ACATCAGCCA GTTGACTAAA CAGCGTCTGG TGGATGCAGA TGGCATCATT AATCCCAGCG 
     3781 CTTTCTACAT CTACCTGACG GCTTGGGTCA GCAACGACCC CGTCGCGTAT GCTGCCTCCC 
     3841 AGGCCAACAT CCGGCCACAC CGACCAGAAT GGGTCCACGA CAAAGCCGAC TACATGCCTG 
     3901 AAACAAGGCT GAGAATCCCG GCAGCAGAGC CCATCGAGTA TGCCCAGTTC CCTTTCTACC 
     3961 TCAACGGCTT GCGGGACACC TCAGACTTTG TGGAGGCAAT TGAAAAAGTA AGGACCATCT 
     4021 GCAGCAACTA TACGAGCCTG GGGCTGTCCA GTTACCCCAA CGGCTACCCC TTCCTCTTCT 
     4081 GGGAGCAGTA CATCGGCCTC CGCCACTGGC TGCTGCTGTT CATCAGCGTG GTGTTGGCCT 
     4141 GCACATTCCT CGTGTGCGCT GTCTTCCTTC TGAACCCCTG GACGGCCGGG ATCATTGTGA 
     4201 TGGTCCTGGC GCTGATGACG GTCGAGCTGT TCGGCATGAT GGGCCTCATC GGAATCAAGC 
     4261 TCAGTGCCGT GCCCGTGGTC ATCCTGATCG CTTCTGTTGG CATAGGAGTG GAGTTCACCG 
     4321 TTCACGTTGC TTTGGCCTTT CTGACGGCCA TCGGCGACAA GAACCGCAGG GCTGTGCTTG 
     4381 CCCTGGAGCA CATGTTTGCA CCCGTCCTGG ATGGCGCCGT GTCCACTCTG CTGGGAGTGC 
     4441 TGATGCTGGC GGGATCTGAG TTCGACTTCA TTGTCAGGTA TTTCTTTGCT GTGCTGGCGA 
     4501 TCCTCACCAT CCTCGGCGTT CTCAATGGGC TGGTTTTGCT TCCCGTGCTT TTGTCTTTCT 
     4561 TTGGACCATA TCCTGAGGTG TCTCCAGCCA ACGGCTTGAA CCGCCTGCCC ACACCCTCCC 
     4621 CTGAGCCACC CCCCAGCGTG GTCCGCTTCG CCATGCCGCC CGGCCACACG CACAGCGGGT 
     4681 CTGATTCCTC CGACTCGGAG TATAGTTCCC AGACGACAGT GTCAGGCCTC AGCGAGGAGC 
     4741 TTCGGCACTA CGAGGCCCAG CAGGGCGCGG GAGGCCCTGC CCACCAAGTG ATCGTGGAAG 
     4801 CCACAGAAAA CCCCGTCTTC GCCCACTCCA CTGTGGTCCA TCCCGAATCC AGGCATCACC 
     4861 CACCCTCGAA CCCGAGACAG CAGCCCCACC TGGACTCAGG GTCCCTGCCT CCCGGACGGC 
     4921 AAGGCCAGCA GCCCCGCAGG GACCCCCCCA GAGAAGGCTT GTGGCCACCC CCCTACAGAC 
     4981 CGCGCAGAGA CGCTTTTGAA ATTTCTACTG AAGGGCATTC TGGCCCTAGC AATAGGGCCC 
     5041 GCTGGGGCCC TCGCGGGGCC CGTTCTCACA ACCCTCGGAA CCCAGCGTCC ACTGCCATGG 
     5101 GCAGCTCCGT GCCCGGCTAC TGCCAGCCCA TCACCACTGT GACGGCTTCT GCCTCCGTGA 
     5161 CTGTCGCCGT GCACCCGCCG CCTGTCCCTG GGCCTGGGCG GAACCCCCGA GGGGGACTCT 
     5221 GCCCAGGCTA CCCTGAGACT GACCACGGCC TGTTTGAGGA CCCCCACGTG CCTTTCCACG 
     5281 TCCGGTGTGA GAGGAGGGAT TCGAAGGTGG AAGTCATTGA GCTGCAGGAC GTGGAATGCG 
     5341 AGGAGAGGCC CCGGGGAAGC AGCTCCAACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5401 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5461 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5521 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5581 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5641 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5701 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5761 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5821 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5881 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5941 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6001 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6061 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6121 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6181 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6241 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6301 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6361 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6421 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6481 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6541 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6601 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6661 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6721 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6781 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6841 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6901 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6961 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7021 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7081 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7141 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7201 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7261 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7321 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7381 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7441 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7501 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7561 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7621 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7681 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7741 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7801 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7861 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7921 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7981 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8041 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8101 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8161 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8221 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8281 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8341 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8401 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8461 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8521 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8641 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8701 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8761 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8821 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8881 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8941 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9001 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9061 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9121 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9181 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//