LOCUS       RC216948                7914 bp    DNA     circular  SYN 
DEFINITION  LNPEP (Myc-DDK-tagged)-Human leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 2
ACCESSION   NM_175920
VERSION     NM_175920.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4071..4162 
                     /label="Myc-DDK"  
     misc_feature    4192..4777 
                     /label="PolyA signal"  
     misc_feature    4249..4268 
                     /label="Restriction Enzyme"  
     misc_feature    5053..5725 
                     /label="ColE1"  
     misc_feature    6155..6949 
                     /label="Kan/Neo"  
     misc_feature    6984..7286 
                     /label="SV40 ori"  
     misc_feature    7372..7849 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4053..4059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4053 
                     /label="LNPEP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGAAAAC AGCATGTTTG AGGAAGAACC AGATGTGGTG GATTTAGCCA 
     1081 AAGAGCCTTG TTTACATCCT CTAGAGCCTG ATGAGGTGGA ATATGAGCCC CGGGGTTCCC 
     1141 GACTGCTGGT GCGGGGTCTT GGTGAGCATG AGATGGAGGA GGATGAAGAG GATTATGAGT 
     1201 CATCAGCAAA GCTGCTGGGC ATGTCCTTCA TGAATAGAAG CTCAGGCCTT CGGAACAGTG 
     1261 CAACTGGTTA CAGGCAGAGC CCAGATGGGG CTTGTTCAGT ACCCTCTGCA AGGACCATGG 
     1321 TGGTCTGTGC TTTTGTCATC GTGGTTGCTG TTTCTGTAAT CATGGTGATT TACTTACTGC 
     1381 CCAGATGTAC CTTTACCAAA GAAGGCTGCC ATAAAAAAAA CCAGTCAATT GGACTAATTC 
     1441 AGCCATTTGC AACAAATGGG AAATTGTTTC CATGGGCACA GATCAGGCTT CCCACTGCCG 
     1501 TTGTGCCACT ACGCTATGAA CTCAGCCTAC ACCCGAACCT AACCTCGATG ACATTCAGGG 
     1561 GTTCTGTGAC AATTTCAGTT CAGGCTCTTC AGGTCACATG GAATATCATT CTTCATAGCA 
     1621 CAGGTCATAA TATTTCAAGA GTGACCTTTA TGTCAGCAGT TTCAAGCCAA GAAAAACAAG 
     1681 CTGAGATCCT GGAATATGCA TATCATGGAC AGATCGCCAT TGTTGCCCCC GAAGCCCTTC 
     1741 TAGCAGGGCA CAATTATACG TTGAAGATAG AGTACTCGGC AAATATATCT AGTTCTTATT 
     1801 ATGGGTTTTA TGGCTTCTCC TACACAGATG AAAGTAATGA GAAAAAGTAC TTTGCAGCAA 
     1861 CTCAGTTTGA ACCCCTGGCA GCAAGATCTG CTTTTCCTTG TTTTGATGAA CCAGCATTTA 
     1921 AAGCCACTTT TATCATCAAG ATCATAAGGG ATGAGCAATA CACCGCTTTA TCAAATATGC 
     1981 CTAAGAAGTC ATCAGTCGTT CTAGATGATG GACTTGTTCA GGATGAGTTT TCTGAGAGTG 
     2041 TGAAGATGAG CACTTACTTG GTTGCTTTCA TTGTGGGAGA GATGAAGAAC CTGAGTCAGG 
     2101 ACGTAAATGG AACCCTGGTT TCTATATATG CTGTACCAGA AAAGATTGGT CAAGTTCATT 
     2161 ATGCCTTGGA AACAACTGTG AAGCTTCTTG AGTTTTTTCA AAACTACTTT GAAATTCAGT 
     2221 ACCCACTTAA GAAATTGGAT TTGGTGGCTA TTCCTGACTT TGAAGCAGGA GCAATGGAAA 
     2281 ATTGGGGTTT GCTCACCTTC CGAGAGGAGA CACTTCTGTA TGACAGTAAC ACTTCTTCAA 
     2341 TGGCGGATAG AAAGCTGGTG ACTAAAATCA TTGCTCATGA GCTGGCCCAC CAGTGGTTTG 
     2401 GCAATCTGGT AACAATGAAG TGGTGGAATG ACCTATGGCT AAATGAAGGT TTTGCCACTT 
     2461 TCATGGAGTA TTTCTCTTTG GAAAAAATAT TCAAAGAGCT TTCTAGTTAT GAAGATTTCT 
     2521 TAGATGCTCG ATTTAAAACC ATGAAGAAAG ATTCCTTAAA TTCATCTCAT CCAATATCAT 
     2581 CATCTGTTCA GTCTTCAGAA CAAATTGAAG AAATGTTTGA TTCTCTTTCC TATTTTAAGG 
     2641 GATCTTCTCT CTTGTTGATG TTGAAAACTT ACCTTAGTGA AGATGTGTTT CAACATGCTG 
     2701 TTGTCCTTTA CCTGCATAAT CACAGCTATG CATCTATTCA AAGTGATGAT CTGTGGGATA 
     2761 GTTTTAATGA GGTCACAAAC CAAACACTAG ATGTAAAGAG AATGATGAAA ACCTGGACCC 
     2821 TGCAGAAAGG ATTTCCTTTA GTGACTGTTC AAAAGAAAGG AAAGGAACTT TTTATACAAC 
     2881 AAGAGAGATT CTTTTTAAAT ATGAAGCCTG AAATTCAGCC TTCAGATACA AGCTACCTGT 
     2941 GGCATATTCC ACTATCCTAT GTCACTGAAG GAAGAAATTA TTCAAAATAT CAATCGGTAT 
     3001 CATTACTGGA TAAGAAATCA GGTGTCATCA ATCTTACAGA AGAAGTGCTG TGGGTCAAAG 
     3061 TGAATATAAA CATGAATGGT TATTATATTG TACACTATGC AGATGATGAT TGGGAAGCAC 
     3121 TAATCCATCA GTTGAAAATA AATCCTTATG TTCTGAGTGA CAAAGACCGA GCCAACCTTA 
     3181 TCAACAACAT CTTTGAACTT GCAGGCCTAG GCAAGGTACC TCTCAAGAGG GCCTTTGATT 
     3241 TGATTAATTA TCTTGGAAAT GAGAACCATA CTGCACCCAT CACCGAAGCC CTGTTTCAGA 
     3301 CAGACCTCAT CTATAACCTC CTTGAAAAAC TGGGATACAT GGATCTGGCC TCAAGACTGG 
     3361 TGACTAGGGT ATTTAAATTA CTTCAAAACC AAATTCAACA ACAAACTTGG ACTGATGAGG 
     3421 GCACTCCATC TATGCGAGAG CTTCGGTCAG CCCTGCTAGA GTTTGCTTGC ACCCACAACC 
     3481 TGGGGAACTG CTCTACTACT GCCATGAAAC TGTTTGATGA CTGGATGGCA TCCAATGGAA 
     3541 CTCAAAGCCT ACCTACTGAT GTCATGACAA CTGTGTTCAA AGTTGGAGCA AAAACTGACA 
     3601 AAGGCTGGTC ATTCCTTTTG GGCAAATACA TTTCTATAGG CTCTGAAGCA GAGAAGAACA 
     3661 AAATACTAGA AGCACTTGCC AGCTCAGAGG ATGTGCGGAA GCTTTACTGG TTAATGAAAA 
     3721 GTAGCCTGAA TGGAGATAAC TTCCGAACAC AGAAGCTGTC TTTTATCATT AGAACAGTGG 
     3781 GTCGACATTT TCCTGGACAC TTACTGGCAT GGGATTTTGT CAAAGAGAAC TGGAATAAGC 
     3841 TTGTACAGAA GTTCCCTCTG GGGTCCTATA CCATACAAAA TATTGTTGCT GGATCAACTT 
     3901 ACCTGTTTTC AACAAAGACA CATTTATCTG AGGTTCAGGC ATTCTTTGAA AATCAGTCAG 
     3961 AGGCAACCTT CCGGCTTCGT TGTGTCCAGG AGGCTTTGGA AGTCATTCAG TTGAATATCC 
     4021 AGTGGATGGA GAAGAACCTC AAAAGTCTCA CATGGTGGCT GACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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