LOCUS       RC216937                8373 bp    DNA     circular  SYN 
DEFINITION  DGKH (Myc-DDK-tagged)-Human diacylglycerol kinase, eta (DGKH), transcript variant 1
ACCESSION   NM_152910
VERSION     NM_152910.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="DGKH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGGGCC GGAGGCCAGC ACCACCCTCC GGGCGCCGCT GGAGGAGCGG 
     1081 CCGCCGGAGC CGGCGCCGCG GTCACCTCCG CCGCTGCCTC GGCGGGGCCG GGAGAGGATT 
     1141 CGTCTGACAG CGAAGCGGAG CAAGAGGGAC CCCAGAAACT GATCCGCAAA GTGTCTACCT 
     1201 CGGGGCAGAT CCGGACCAAG ACCAGTATTA AAGAGGGACA GCTATTGAAG CAAACCAGTT 
     1261 CTTTCCAAAG GTGGAAAAAG CGATACTTCA AACTTCGAGG CCGCACCCTT TACTATGCAA 
     1321 AGGACTCAAA GTCTCTGATA TTTGATGAAG TTGACCTCTC AGATGCTAGT GTAGCTGAAG 
     1381 CAAGCACGAA AAATGCTAAC AACAGCTTCA CGATCATCAC TCCATTCAGA AGGCTAATGC 
     1441 TGTGTGCTGA GAACAGAAAG GAGATGGAGG ATTGGATCAG CTCACTGAAG TCTGTACAGA 
     1501 CCAGAGAACC CTACGAGGTG GCCCAGTTTA ATGTGGAACA TTTCTCAGGG ATGCACAACT 
     1561 GGTACGCCTG CTCCCACGCC CGACCCACCT TCTGTAACGT GTGCAGAGAG AGTCTTTCTG 
     1621 GAGTCACCTC CCATGGCCTG TCCTGCGAAG TGTGTAAATT CAAGGCTCAC AAAAGATGTG 
     1681 CAGTGAGAGC AACAAATAAC TGTAAATGGA CTACCCTGGC CTCCATCGGG AAGGACATTA 
     1741 TAGAAGATGA AGATGGCGTC GCGATGCCTC ACCAGTGGCT TGAGGGCAAC CTGCCTGTAA 
     1801 GTGCCAAGTG TGCTGTCTGC GACAAAACAT GTGGCAGTGT TCTCCGTCTA CAGGATTGGA 
     1861 AATGCCTTTG GTGTAAGACA ATGGTACACA CTGCCTGCAA AGATTTATAC CATCCAATAT 
     1921 GTCCACTTGG TCAATGTAAA GTATCTATCA TACCTCCAAT TGCACTAAAC AGCACCGATT 
     1981 CCGATGGTTT CTGTAGAGCA ACATTTTCGT TCTGTGTTAG TCCTCTATTG GTTTTTGTCA 
     2041 ATTCTAAGAG TGGAGATAAT CAGGGAGTAA AGTTCCTCCG TCGCTTTAAA CAGTTGCTAA 
     2101 ATCCGGCTCA GGTGTTTGAT TTAATGAATG GAGGTCCTCA TTTAGGTTTA AGATTATTTC 
     2161 AGAAGTTTGA CAATTTCCGG ATTCTTGTTT GTGGAGGCGA TGGAAGTGTA GGTTGGGTTT 
     2221 TGTCAGAAAT CGATAAGCTC AACTTGAATA AACAGTGTCA GCTGGGAGTG TTGCCTTTGG 
     2281 GTACAGGAAA TGACCTTGCC CGAGTTCTTG GCTGGGGAGG TTCATATGAC GATGACACCC 
     2341 AACTTCCTCA GATCCTAGAG AAACTGGAAC GAGCCAGTAC CAAAATGTTG GACAGGTGGA 
     2401 GTATAATGAC ATATGAACTC AAATTGCCAC CAAAAGCTTC CCTACTTCCA GGACCTCCAG 
     2461 AAGCATCTGA AGAATTTTAT ATGACGATTT ATGAAGACTC AGTTGCAACG CATCTTACAA 
     2521 AAATCCTCAA TTCTGATGAA CATGCAGTGG TCATATCTTC TGCCAAGACG CTATGTGAAA 
     2581 CTGTAAAGGA CTTCGTTGCC AAAGTAGAAA AGACGTATGA CAAAACCTTG GAAAATGCCG 
     2641 TTGTAGCTGA TGCCGTGGCC AGTAAATGTT CAGTCCTAAA CGAGAAGCTC GAACAACTGC 
     2701 TGCAGGCTTT GCACACAGAT TCCCAGGCTG CGCCTGTTCT CCCTGGCCTC AGCCCTCTCA 
     2761 TTGTGGAAGA AGATGCTGTG GAATCGTCCA GTGAAGAGTC CCTGGGTGAA AGCAAAGAGC 
     2821 AGCTTGGGGA TGACGTTACA AAACCTTCCT CCCAGAAAGC CGTCAAACCA AGGGAAATCA 
     2881 TGTTGCGGGC AAATAGTTTA AAGAAAGCAG TGAGGCAAGT CATTGAGGAA GCCGGAAAAG 
     2941 TTATGGATGA CCCGACAGTT CACCCCTGTG AACCAGCTAA TCAGTCCTCT GATTATGACA 
     3001 GCACAGAAAC AGATGAATCT AAGGAGGAAG CTAAAGATGA TGGTGCCAAA GAATCAATAA 
     3061 CTGTTAAAAC TGCACCTCGG TCTCCAGATG CCCGGGCAAG TTATGGCCAT TCCCAAACTG 
     3121 ATTCTGTCCC TGGTCCAGCT GTGGCAGCCA GCAAAGAAAA CCTCCCTGTG CTCAATACCA 
     3181 GAATAATCTG CCCAGGTTTA AGAGCAGGAC TGGCTGCCTC AATTGCTGGG AGTTCGATTA 
     3241 TCAACAAAAT GTTACTGGCA AACATTGATC CTTTTGGTGC CACGCCGTTT ATTGACCCGG 
     3301 ATCTAGATTC CGTAGATGGA TATTCAGAAA AATGTGTCAT GAACAATTAC TTTGGGATTG 
     3361 GATTAGATGC AAAAATTTCA TTAGAATTTA ATAATAAAAG AGAGGAGCAC CCTGAAAAAT 
     3421 GCAGGAGCCG AACTAAAAAC TTGATGTGGT ATGGAGTCCT TGGAACCCGG GAGTTATTAC 
     3481 AGAGATCGTA CAAGAATTTA GAACAAAGGG TTCAACTTGA GTGTGATGGG CAGTATATTC 
     3541 CTCTTCCCAG CTTGCAAGGC ATAGCCGTGT TGAACATTCC CAGCTATGCT GGAGGCACTA 
     3601 ACTTTTGGGG TGGAACTAAA GAGGATGATA TATTTGCTGC ACCATCCTTT GATGACAAGA 
     3661 TCCTGGAAGT TGTAGCAATA TTTGATAGCA TGCAAATGGC AGTTTCAAGG GTCATTAAAC 
     3721 TGCAGCATCA TCGAATAGCC CAGTGCCGTA CAGTGAAAAT CACTATATTT GGTGACGAAG 
     3781 GAGTCCCAGT GCAAGTGGAT GGTGAAGCGT GGGTTCAGCC TCCAGGGATT ATCAAAATTG 
     3841 TGCACAAAAA CAGAGCACAA ATGCTAACAA GGGACAGAGC CTTTGAGAGC ACTCTGAAAT 
     3901 CTTGGGAAGA TAAGCAGAAG TGTGATTCTG GTAAACCAGT TCTCCGAACC CATTTGTACA 
     3961 TCCATCACGC CATTGACTTG GCAACAGAAG AGGTGTCGCA GATGCAGCTA TGCTCCCAGG 
     4021 CTGCAGAGGA GCTCATTACT AGGATATGTG ACGCAGCCAC AATTCACTGT CTTTTGGAGC 
     4081 AAGAACTGGC CCATGCTGTG AATGCCTGCT CCCATGCCCT GAATAAAGCC AACCCAAGGT 
     4141 GCCCGGAGAG TCTTACAAGA GACACTGCCA CTGAAATAGC CATCAATGTG AAGGCGCTGT 
     4201 ATAATGAAAC AGAATCTTTG CTAGTTGGCA GGGTTCCTTT GCAGCTGGAA TCGCCACATG 
     4261 AAGAGCGAGT ATCCAATGCC TTACACTCTG TGGAGGTGGA ATTACAGAAA CTGACAGAGA 
     4321 TTCCTTGGCT TTATTATATC TTACACCCAA ATGAGGATGA GGAACCTCCT ATGGATTGCA 
     4381 CCAAAAGGAA CAACAGAAGC ACCGTATTTC GAATAGTGCC AAAGTTTAAA AAGGAAAAGG 
     4441 TTCAGAAGCA GAAGACAAGT TCACAGCCTG GATCTGGGGA TACCGAAAGT GGGTCATGTG 
     4501 AAGCGAATTC TCCAGGGAAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//