LOCUS       RC216926                8235 bp    DNA     circular  SYN 
DEFINITION  USP8 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 8 (USP8), transcript variant 1
ACCESSION   NM_005154
VERSION     NM_005154.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4392..4483 
                     /label="Myc-DDK"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    4570..4589 
                     /label="Restriction Enzyme"  
     misc_feature    5374..6046 
                     /label="ColE1"  
     misc_feature    6476..7270 
                     /label="Kan/Neo"  
     misc_feature    7305..7607 
                     /label="SV40 ori"  
     misc_feature    7693..8170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="USP8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCTGTG GCTTCAGTTC CTAAAGAACT CTACCTCAGT TCTTCACTAA 
     1081 AAGACCTTAA TAAGAAGACA GAAGTTAAAC CAGAGAAAAT AAGCACTAAG AGTTATGTGC 
     1141 ACAGTGCCCT GAAGATCTTT AAGACAGCAG AAGAATGCAG ATTAGATCGT GATGAGGAAA 
     1201 GGGCCTATGT ACTATATATG AAATACGTGA CTGTTTATAA TCTTATCAAA AAAAGACCTG 
     1261 ATTTCAAGCA ACAGCAGGAT TATTTCCATT CAATACTTGG ACCTGGAAAC ATCAAAAAAG 
     1321 CTGTCGAAGA AGCTGAAAGA CTCTCTGAAA GCCTTAAATT AAGATATGAA GAAGCTGAAG 
     1381 TCCGGAAAAA ACTTGAGGAA AAAGACAGGC AGGAGGAAGC ACAGCGGCTA CAACAAAAAA 
     1441 GGCAGGAAAC AGGAAGAGAG GATGGTGGCA CATTGGCTAA AGGCTCTTTG GAGAATGTTT 
     1501 TGGATTCCAA AGACAAAACC CAAAAGAGCA ATGGTGAAAA GAATGAAAAA TGTGAGACCA 
     1561 AAGAGAAAGG AGCAATCACA GCAAAGGAAC TATACACAAT GATGACGGAT AAAAACATCA 
     1621 GCTTGATTAT AATGGATGCT CGAAGAATGC AGGATTATCA GGATTCCTGT ATTTTACATT 
     1681 CTCTCAGTGT TCCTGAAGAA GCCATCAGTC CAGGAGTCAC TGCTAGTTGG ATTGAAGCAC 
     1741 ACCTGCCAGA TGATTCTAAA GACACATGGA AGAAGAGGGG GAATGTGGAG TATGTGGTAC 
     1801 TTCTTGACTG GTTTAGTTCT GCCAAAGATT TACAGATTGG AACAACTCTC CGGAGTCTGA 
     1861 AAGATGCACT TTTCAAGTGG GAAAGTAAAA CTGTCCTGCG CAATGAGCCT TTGGTTTTAG 
     1921 AGGGAGGCTA TGAAAACTGG CTCCTTTGTT ATCCCCAGTA TACAACAAAT GCTAAGGTCA 
     1981 CTCCACCCCC ACGACGCCAG AATGAAGAGG TGTCTATCTC ATTGGATTTT ACTTATCCCT 
     2041 CATTGGAAGA ATCAATTCCT TCTAAACCTG CTGCCCAGAC GCCACCTGCA TCTATAGAAG 
     2101 TAGATGAAAA TATAGAATTG ATAAGTGGTC AAAATGAGAG AATGGGACCA CTGAATATAT 
     2161 CAACTCCAGT TGAACCAGTT GCTGCTTCTA AATCTGATGT TTCACCCATA ATTCAGCCAG 
     2221 TGCCTAGTAT AAAGAATGTT CCACAGATTG ATCGTACTAA AAAACCAGCA GTCAAATTGC 
     2281 CTGAAGAGCA TAGAATAAAA TCTGAAAGTA CAAACCATGA GCAACAATCT CCTCAGAGTG 
     2341 GAAAAGTTAT TCCTGATCGT TCCACCAAGC CAGTAGTTTT TTCTCCAACT CTCATGTTAA 
     2401 CAGATGAAGA AAAGGCTCGT ATTCATGCAG AAACTGCTCT TCTAATGGAA AAAAACAAAC 
     2461 AAGAAAAAGA ACTTCGGGAA AGGCAGCAAG AGGAACAGAA AGAGAAACTG AGGAAGGAAG 
     2521 AACAAGAACA AAAAGCCAAA AAGAAACAAG AAGCTGAAGA AAATGAAATT ACAGAGAAGC 
     2581 AACAAAAAGC AAAAGAAGAA ATGGAGAAGA AAGAAAGTGA ACAGGCCAAG AAAGAAGATA 
     2641 AAGAAACCTC AGCAAAGAGG GGCAAAGAAA TAACAGGAGT AAAAAGACAA AGTAAAAGTG 
     2701 AACATGAAAC TTCTGATGCC AAGAAATCTG TAGAAGATAG GGGGAAAAGG TGTCCAACCC 
     2761 CAGAAATACA GAAAAAGTCA ACAGGAGATG TGCCCCATAC ATCTGTGACA GGGGATTCAG 
     2821 GTTCAGGCAA GCCATTTAAG ATTAAAGGAC AACCAGAAAG TGGAATTCTA AGGACAGGAA 
     2881 CTTTTAGAGA GGATACAGAC GATACCGAAA GAAATAAAGC TCAACGAGAA CCTTTGACAA 
     2941 GAGCACGAAG TGAAGAAATG GGGAGGATCG TACCAGGACT GCCTTCAGGC TGGGCCAAGT 
     3001 TTCTTGACCC AATCACTGGA ACCTTTCGTT ATTATCATTC ACCCACCAAC ACTGTTCATA 
     3061 TGTACCCACC GGAAATGGCT CCTTCATCTG CACCTCCTTC CACCCCTCCA ACTCATAAAG 
     3121 CCAAGCCACA GATTCCTGCT GAGCGGGATA GGGAACCTTC CAAACTGAAG CGCTCCTACT 
     3181 CCTCCCCAGA TATAACCCAG GCTATTCAAG AGGAAGAGAA GAGGAAGCCA ACAGTAACTC 
     3241 CAACAGTTAA TCGGGAAAAC AAGCCAACAT GTTATCCTAA AGCTGAGATC TCAAGGCTTT 
     3301 CTGCTTCTCA GATTCGGAAC CTCAATCCTG TTTTTGGAGG TTCTGGACCA GCTCTTACTG 
     3361 GACTTCGTAA CTTAGGAAAT ACTTGTTATA TGAACTCAAT ATTGCAGTGC CTATGTAACG 
     3421 CTCCACATTT GGCTGATTAT TTCAACCGAA ACTGTTATCA GGATGATATT AACAGGTCAA 
     3481 ATTTGTTGGG GCATAAAGGT GAAGTGGCAG AAGAATTTGG TATAATCATG AAAGCCCTGT 
     3541 GGACAGGACA GTATAGATAT ATCAGTCCAA AGGACTTTAA AATCACCATT GGGAAGATCA 
     3601 ATGACCAGTT TGCAGGATAC AGTCAGCAAG ATTCACAAGA ATTGCTTCTG TTCCTAATGG 
     3661 ATGGTCTCCA TGAAGATCTA AATAAAGCTG ATAATCGGAA GAGATATAAA GAAGAAAATA 
     3721 ATGATCATCT CGATGACTTT AAAGCTGCAG AACATGCCTG GCAGAAACAC AAGCAGCTCA 
     3781 ATGAGTCTAT TATTGTTGCA CTTTTTCAGG GTCAATTCAA ATCTACAGTA CAGTGCCTCA 
     3841 CATGTCACAA AAAGTCTAGG ACATTTGAGG CCTTCATGTA TTTGTCTCTA CCACTAGCAT 
     3901 CCACAAGTAA ATGTACATTA CAGGATTGCC TTAGATTATT TTCCAAAGAA GAAAAACTCA 
     3961 CAGATAACAA CAGATTTTAC TGCAGTCATT GCAGAGCTCG ACGGGATTCT CTAAAAAAGA 
     4021 TAGAAATCTG GAAGTTACCA CCTGTGCTTT TAGTGCATCT GAAACGTTTT TCCTACGATG 
     4081 GCAGGTGGAA ACAAAAATTA CAGACATCTG TGGACTTCCC GTTAGAAAAT CTTGACTTGT 
     4141 CACAGTATGT TATTGGTCCA AAGAACAATT TGAAGAAATA TAATTTGTTT TCTGTTTCAA 
     4201 ATCACTACGG TGGGCTGGAT GGAGGCCACT ACACAGCCTA TTGTAAAAAT GCAGCAAGAC 
     4261 AACGGTGGTT TAAGTTTGAT GATCATGAAG TTTCTGATAT CTCCGTTTCT TCTGTGAAAT 
     4321 CTTCAGCAGC TTATATCCTC TTTTATACTT CATTGGGACC ACGAGTAACT GATGTAGCCA 
     4381 CAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//