LOCUS       RC216918                7989 bp    DNA     circular  SYN 
DEFINITION  COL6A3 (Myc-DDK-tagged)-Human collagen, type VI, alpha 3 (COL6A3), transcript variant 2
ACCESSION   NM_057164
VERSION     NM_057164.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="COL6A3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAAACAT CGGCACTTGC CCTTAGTGGC CGTCTTTTGC CTCTTTCTCT 
     1081 CAGGCTTTCC TACAACTCAT GCCCAGCAGC AGCAAGCAGT CATTGAAGTC AACAAGAGAG 
     1141 ACATAGTCTT CCTGGTGGAT GGCTCATCTG CACTGGGACT GGCCAACTTC AATGCCATCC 
     1201 GAGACTTCAT TGCTAAAGTC ATCCAGAGGC TGGAAATCGG ACAGGATCTT ATCCAGGTGG 
     1261 CAGTGGCCCA GTATGCAGAC ACTGTGAGGC CTGAATTTTA TTTCAATACC CATCCAACAA 
     1321 AAAGGGAAGT CATAACCGCT GTGCGGAAAA TGAAGCCCCT GGACGGCTCG GCCCTGTACA 
     1381 CGGGCTCTGC TCTAGACTTT GTTCGTAACA ACCTATTCAC GAGTTCAGCC GGCTACCGGG 
     1441 CTGCCGAGGG GATTCCTAAG CTTTTGGTGC TGATCACAGG TGGTAAGTCC CTAGATGAAA 
     1501 TCAGCCAGCC TGCCCAGGAG CTGAAGAGAA GCAGCATAAT GGCCTTTGCC ATTGGGAACA 
     1561 AGGGTGCCGA TCAGGCTGAG CTGGAAGAGA TCGCTTTCGA CTCCTCCCTG GTGTTCATCC 
     1621 CAGCTGAGTT CCGAGCCGCC CCATTGCAAG GCATGCTGCC TGGCTTGCTG GCACCTCTCA 
     1681 GGACCCTCTC TGGAACCCCT GAAGTTCACT CAAACAAAAG GGATATCATC TTTCTTTTGG 
     1741 ATGGATCAGC CAACGTTGGA AAAACCAATT TCCCTTATGT GCGCGACTTT GTAATGAACC 
     1801 TAGTTAACAG CCTTGATATT GGAAATGACA ATATTCGTGT TGGTTTAGTG CAATTTAGTG 
     1861 ACACTCCTGT AACGGAGTTC TCTTTAAACA CATACCAGAC CAAGTCAGAT ATCCTTGGTC 
     1921 ATCTGAGGCA GCTGCAGCTC CAGGGAGGTT CGGGCCTGAA CACAGGCTCA GCCCTAAGCT 
     1981 ATGTCTATGC CAACCACTTC ACGGAAGCTG GCGGCAGCAG GATCCGTGAA CACGTGCCGC 
     2041 AGCTCCTGCT TCTGCTCACA GCTGGGCAGT CTGAGGACTC CTATTTGCAA GCTGCCAACG 
     2101 CCTTGACACG CGCGGGCATC CTGACTTTTT GTGTGGGAGC TAGCCAGGCG AATAAGGCAG 
     2161 AGCTTGAGCA GATTGCTTTT AACCCAAGCC TGGTGTATCT CATGGATGAT TTCAGCTCCC 
     2221 TGCCAGCTTT GCCTCAGCAG CTGATTCAGC CCCTAACCAC ATATGTTAGT GGAGGTGTGG 
     2281 AGGAAGTACC ACTCGCTCAG CCAGAGAGCA AGCGAGACAT TCTGTTCCTC TTTGACGGCT 
     2341 CAGCCAATCT TGTGGGCCAG TTCCCTGTTG TCCGTGACTT TCTCTACAAG ATTATCGATG 
     2401 AGCTCAATGT GAAGCCAGAG GGGACCCGAA TTGCGGTGGC TCAGTACAGC GATGATGTCA 
     2461 AGGTGGAGTC CCGTTTTGAT GAGCACCAGA GTAAGCCTGA GATCCTGAAT CTTGTGAAGA 
     2521 GAATGAAGAT CAAGACGGGC AAAGCCCTCA ACCTGGGCTA CGCGCTGGAC TATGCACAGA 
     2581 GGTACATTTT TGTGAAGTCT GCTGGCAGCC GGATCGAGGA TGGAGTGCTT CAGTTCCTGG 
     2641 TGCTGCTGGT CGCAGGAAGG TCATCTGACC GTGTGGATGG GCCAGCAAGT AACCTGAAGC 
     2701 AGAGTGGGGT TGTGCCTTTC ATCTTCCAAG CCAAGAACGC AGACCCTGCT GAGTTAGAGC 
     2761 AGATCGTGCT GTCTCCAGCG TTTATCCTGG CTGCAGAGTC GCTTCCCAAG ATTGGAGATC 
     2821 TTCATCCACA GATAGTGAAT CTCTTAAAAT CAGTGCACAA CGGAGCACCA GCACCAGTTT 
     2881 CAGGTGAAAA GGACGTGGTG TTTCTGCTTG ATGGCTCTGA GGGCGTCAGG AGCGGCTTCC 
     2941 CTCTGTTGAA AGAGTTTGTC CAGAGAGTGG TGGAAAGCCT GGATGTGGGC CAGGACCGGG 
     3001 TCCGCGTGGC CGTGGTGCAG TACAGCGACC GGACCAGGCC CGAGTTCTAC CTGAATTCAT 
     3061 ACATGAACAA GCAGGACGTC GTCAACGCTG TCCGCCAGCT GACCCTGCTG GGAGGGCCGA 
     3121 CCCCCAACAC CGGGGCCGCC CTGGAGTTTG TCCTGAGGAA CATCCTGGTC AGCTCTGCGG 
     3181 GAAGCAGGAT AACAGAAGGT GTGCCCCAGC TGCTGATCGT CCTCACGGCC GACAGGTCTG 
     3241 GGGATGATGT GCGGAACCCC TCCGTGGTCG TGAAGAGGGG TGGGGCTGTG CCCATTGGCA 
     3301 TTGGCATCGG GAACGCTGAC ATCACAGAGA TGCAGACCAT CTCCTTCATC CCGGACTTTG 
     3361 CCGTGGCCAT TCCCACCTTT CGCCAGCTGG GGACCGTCCA ACAGGTCATC TCTGAGAGGG 
     3421 TGACCCAGCT CACCCGCGAG GAGCTGAGCA GGCTGCAGCC GGTGTTGCAG CCTCTACCGA 
     3481 GCCCAGGTGT TGGTGGCAAG AGGGACGTGG TCTTTCTCAT CGATGGGTCC CAAAGTGCCG 
     3541 GGCCTGAGTT CCAGTACGTT CGCACCCTCA TAGAGAGGCT GGTTGACTAC CTGGACGTGG 
     3601 GCTTTGACAC CACCCGGGTG GCTGTCATCC AGTTCAGCGA TGACCCCAAG GTGGAGTTCC 
     3661 TGCTGAACGC CCATTCCAGC AAGGATGAAG TGCAGAACGC GGTGCAGCGG CTGAGGCCCA 
     3721 AGGGAGGGCG GCAGATCAAC GTGGGCAATG CCCTGGAGTA CGTGTCCAGG AACATCTTCA 
     3781 AGAGGCCCCT GGGGAGCCGC ATTGAAGAGG GCGTCCCGCA GTTCCTGGTC CTCATCTCGT 
     3841 CTGGAAAGTC TGACGATGAG GTGGACGACC CGGCGGTGGA GCTCAAGCAG TTTGGCGTGG 
     3901 CCCCTTTCAC GATCGCCAGG AACGCAGACC AGGAGGAGCT GGTGAAGATC TCGCTGAGCC 
     3961 CCGAATATGT GTTCTCGGTG AGCACCTTCC GGGAGCTGCC CAGCCTGGAG CAGAAACTGC 
     4021 TGACGCCCAT CACGACCCTG ACCTCAGAGC AGATCCAGAA GCTCTTAGCC AGCACTCGCT 
     4081 ATCCACCTCC AGGTGAGATG GGGGCGTCGG AGGTTCTCCT TGGAGCATTT TCCATAACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//