LOCUS       RC216897                7338 bp    DNA     circular  SYN 
DEFINITION  KCNH2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 3
ACCESSION   NM_172057
VERSION     NM_172057.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3495..3586 
                     /label="Myc-DDK"  
     misc_feature    3616..4201 
                     /label="PolyA signal"  
     misc_feature    3673..3692 
                     /label="Restriction Enzyme"  
     misc_feature    4477..5149 
                     /label="ColE1"  
     misc_feature    5579..6373 
                     /label="Kan/Neo"  
     misc_feature    6408..6710 
                     /label="SV40 ori"  
     misc_feature    6796..7273 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3477..3483 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3477 
                     /label="KCNH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCCCA GCCGGGAAGG CGAGCAGGAC AGGGGCTCTG CGGCCCAGGG 
     1081 CCCAGAAAGG CCGGGTGAGG CGGGCCGTGC GCATCTCCAG CCTCGTGGCC CAGGAGGTCC 
     1141 TGTCCCTGGG CGCCGACGTG CTGCCTGAGT ACAAGCTGCA GGCACCGCGC ATCCACCGCT 
     1201 GGACCATCCT GCATTACAGC CCCTTCAAGG CCGTGTGGGA CTGGCTCATC CTGCTGCTGG 
     1261 TCATCTACAC GGCTGTCTTC ACACCCTACT CGGCTGCCTT CCTGCTGAAG GAGACGGAAG 
     1321 AAGGCCCGCC TGCTACCGAG TGTGGCTACG CCTGCCAGCC GCTGGCTGTG GTGGACCTCA 
     1381 TCGTGGACAT CATGTTCATT GTGGACATCC TCATCAACTT CCGCACCACC TACGTCAATG 
     1441 CCAACGAGGA GGTGGTCAGC CACCCCGGCC GCATCGCCGT CCACTACTTC AAGGGCTGGT 
     1501 TCCTCATCGA CATGGTGGCC GCCATCCCCT TCGACCTGCT CATCTTCGGC TCTGGCTCTG 
     1561 AGGAGCTGAT CGGGCTGCTG AAGACTGCGC GGCTGCTGCG GCTGGTGCGC GTGGCGCGGA 
     1621 AGCTGGATCG CTACTCAGAG TACGGCGCGG CCGTGCTGTT CTTGCTCATG TGCACCTTTG 
     1681 CGCTCATCGC GCACTGGCTA GCCTGCATCT GGTACGCCAT CGGCAACATG GAGCAGCCAC 
     1741 ACATGGACTC ACGCATCGGC TGGCTGCACA ACCTGGGCGA CCAGATAGGC AAACCCTACA 
     1801 ACAGCAGCGG CCTGGGCGGC CCCTCCATCA AGGACAAGTA TGTGACGGCG CTCTACTTCA 
     1861 CCTTCAGCAG CCTCACCAGT GTGGGCTTCG GCAACGTCTC TCCCAACACC AACTCAGAGA 
     1921 AGATCTTCTC CATCTGCGTC ATGCTCATTG GCTCCCTCAT GTATGCTAGC ATCTTCGGCA 
     1981 ACGTGTCGGC CATCATCCAG CGGCTGTACT CGGGCACAGC CCGCTACCAC ACACAGATGC 
     2041 TGCGGGTGCG GGAGTTCATC CGCTTCCACC AGATCCCCAA TCCCCTGCGC CAGCGCCTCG 
     2101 AGGAGTACTT CCAGCACGCC TGGTCCTACA CCAACGGCAT CGACATGAAC GCGGTGCTGA 
     2161 AGGGCTTCCC TGAGTGCCTG CAGGCTGACA TCTGCCTGCA CCTGAACCGC TCACTGCTGC 
     2221 AGCACTGCAA ACCCTTCCGA GGGGCCACCA AGGGCTGCCT TCGGGCCCTG GCCATGAAGT 
     2281 TCAAGACCAC ACATGCACCG CCAGGGGACA CACTGGTGCA TGCTGGGGAC CTGCTCACCG 
     2341 CCCTGTACTT CATCTCCCGG GGCTCCATCG AGATCCTGCG GGGCGACGTC GTCGTGGCCA 
     2401 TCCTGGGGAA GAATGACATC TTTGGGGAGC CTCTGAACCT GTATGCAAGG CCTGGCAAGT 
     2461 CGAACGGGGA TGTGCGGGCC CTCACCTACT GTGACCTACA CAAGATCCAT CGGGACGACC 
     2521 TGCTGGAGGT GCTGGACATG TACCCTGAGT TCTCCGACCA CTTCTGGTCC AGCCTGGAGA 
     2581 TCACCTTCAA CCTGCGAGAT ACCAACATGA TCCCGGGCTC CCCCGGCAGT ACGGAGTTAG 
     2641 AGGGTGGCTT CAGTCGGCAA CGCAAGCGCA AGTTGTCCTT CCGCAGGCGC ACGGACAAGG 
     2701 ACACGGAGCA GCCAGGGGAG GTGTCGGCCT TGGGGCCGGG CCGGGCGGGG GCAGGGCCGA 
     2761 GTAGCCGGGG CCGGCCGGGG GGGCCGTGGG GGGAGAGCCC GTCCAGTGGC CCCTCCAGCC 
     2821 CTGAGAGCAG TGAGGATGAG GGCCCAGGCC GCAGCTCCAG CCCCCTCCGC CTGGTGCCCT 
     2881 TCTCCAGCCC CAGGCCCCCC GGAGAGCCGC CGGGTGGGGA GCCCCTGATG GAGGACTGCG 
     2941 AGAAGAGCAG CGACACTTGC AACCCCCTGT CAGGCGCCTT CTCAGGAGTG TCCAACATTT 
     3001 TCAGCTTCTG GGGGGACAGT CGGGGCCGCC AGTACCAGGA GCTCCCTCGA TGCCCCGCCC 
     3061 CCACCCCCAG CCTCCTCAAC ATCCCCCTCT CCAGCCCGGG TCGGCGGCCC CGGGGCGACG 
     3121 TGGAGAGCAG GCTGGATGCC CTCCAGCGCC AGCTCAACAG GCTGGAGACC CGGCTGAGTG 
     3181 CAGACATGGC CACTGTCCTG CAGCTGCTAC AGAGGCAGAT GACGCTGGTC CCGCCCGCCT 
     3241 ACAGTGCTGT GACCACCCCG GGGCCTGGCC CCACTTCCAC ATCCCCGCTG TTGCCCGTCA 
     3301 GCCCCCTCCC CACCCTCACC TTGGACTCGC TTTCTCAGGT TTCCCAGTTC ATGGCGTGTG 
     3361 AGGAGCTGCC CCCGGGGGCC CCAGAGCTTC CCCAAGAAGG CCCCACACGA CGCCTCTCCC 
     3421 TACCGGGCCA GCTGGGGGCC CTCACCTCCC AGCCCCTGCA CAGACACGGC TCGGACCCGG 
     3481 GCAGTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3541 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3601 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3661 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3721 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3781 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3841 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3901 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3961 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4021 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4081 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4141 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4201 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4261 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4321 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4381 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4981 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5041 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5101 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5161 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5221 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5281 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5341 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5401 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5461 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5521 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5581 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5641 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5701 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5761 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5821 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5881 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5941 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6001 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6061 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6121 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6181 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6241 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6301 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6361 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6421 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6481 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6541 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6601 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6661 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6721 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6781 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6841 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6901 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6961 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7021 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7081 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7141 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7201 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7261 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7321 AAATTTAACG CGAATTTT
//