LOCUS       RC216888                8523 bp    DNA     circular  SYN 
DEFINITION  TRPM4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant 1
ACCESSION   NM_017636
VERSION     NM_017636.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="TRPM4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGTGCCG GAGAAGGAGC AGAGCTGGAT CCCCAAGATC TTCAAGAAGA 
     1081 AGACCTGCAC GACGTTCATA GTTGACTCCA CAGATCCGGG AGGGACCTTG TGCCAGTGTG 
     1141 GGCGCCCCCG GACCGCCCAC CCCGCAGTGG CCATGGAGGA TGCCTTCGGG GCAGCCGTGG 
     1201 TGACCGTGTG GGACAGCGAT GCACACACCA CGGAGAAGCC CACCGATGCC TACGGAGAGC 
     1261 TGGACTTCAC GGGGGCCGGC CGCAAGCACA GCAATTTCCT CCGGCTCTCT GACCGAACGG 
     1321 ATCCAGCTGC AGTTTATAGT CTGGTCACAC GCACATGGGG CTTCCGTGCC CCGAACCTGG 
     1381 TGGTGTCAGT GCTGGGGGGA TCGGGGGGCC CCGTCCTCCA GACCTGGCTG CAGGACCTGC 
     1441 TGCGTCGTGG GCTGGTGCGG GCTGCCCAGA GCACAGGAGC CTGGATTGTC ACTGGGGGTC 
     1501 TGCACACGGG CATCGGCCGG CATGTTGGTG TGGCTGTACG GGACCATCAG ATGGCCAGCA 
     1561 CTGGGGGCAC CAAGGTGGTG GCCATGGGTG TGGCCCCCTG GGGTGTGGTC CGGAATAGAG 
     1621 ACACCCTCAT CAACCCCAAG GGCTCGTTCC CTGCGAGGTA CCGGTGGCGC GGTGACCCGG 
     1681 AGGACGGGGT CCAGTTTCCC CTGGACTACA ACTACTCGGC CTTCTTCCTG GTGGACGACG 
     1741 GCACACACGG CTGCCTGGGG GGCGAGAACC GCTTCCGCTT GCGCCTGGAG TCCTACATCT 
     1801 CACAGCAGAA GACGGGCGTG GGAGGGACTG GAATTGACAT CCCTGTCCTG CTCCTCCTGA 
     1861 TTGATGGTGA TGAGAAGATG TTGACGCGAA TAGAGAACGC CACCCAGGCT CAGCTCCCAT 
     1921 GTCTCCTCGT GGCTGGCTCA GGGGGAGCTG CGGACTGCCT GGCGGAGACC CTGGAAGACA 
     1981 CTCTGGCCCC AGGGAGTGGG GGAGCCAGGC AAGGCGAAGC CCGAGATCGA ATCAGGCGTT 
     2041 TCTTTCCCAA AGGGGACCTT GAGGTCCTGC AGGCCCAGGT GGAGAGGATT ATGACCCGGA 
     2101 AGGAGCTCCT GACAGTCTAT TCTTCTGAGG ATGGGTCTGA GGAATTCGAG ACCATAGTTT 
     2161 TGAAGGCCCT TGTGAAGGCC TGTGGGAGCT CGGAGGCCTC AGCCTACCTG GATGAGCTGC 
     2221 GTTTGGCTGT GGCTTGGAAC CGCGTGGACA TTGCCCAGAG TGAACTCTTT CGGGGGGACA 
     2281 TCCAATGGCG GTCCTTCCAT CTCGAAGCTT CCCTCATGGA CGCCCTGCTG AATGACCGGC 
     2341 CTGAGTTCGT GCGCTTGCTC ATTTCCCACG GCCTCAGCCT GGGCCACTTC CTGACCCCGA 
     2401 TGCGCCTGGC CCAACTCTAC AGCGCGGCGC CCTCCAACTC GCTCATCCGC AACCTTTTGG 
     2461 ACCAGGCGTC CCACAGCGCA GGCACCAAAG CCCCAGCCCT AAAAGGGGGA GCTGCGGAGC 
     2521 TCCGGCCCCC TGACGTGGGG CATGTGCTGA GGATGCTGCT GGGGAAGATG TGCGCGCCGA 
     2581 GGTACCCCTC CGGGGGCGCC TGGGACCCTC ACCCAGGCCA GGGCTTCGGG GAGAGCATGT 
     2641 ATCTGCTCTC GGACAAGGCC ACCTCGCCGC TCTCGCTGGA TGCTGGCCTC GGGCAGGCCC 
     2701 CCTGGAGCGA CCTGCTTCTT TGGGCACTGT TGCTGAACAG GGCACAGATG GCCATGTACT 
     2761 TCTGGGAGAT GGGTTCCAAT GCAGTTTCCT CAGCTCTTGG GGCCTGTTTG CTGCTCCGGG 
     2821 TGATGGCACG CCTGGAGCCT GACGCTGAGG AGGCAGCACG GAGGAAAGAC CTGGCGTTCA 
     2881 AGTTTGAGGG GATGGGCGTT GACCTCTTTG GCGAGTGCTA TCGCAGCAGT GAGGTGAGGG 
     2941 CTGCCCGCCT CCTCCTCCGT CGCTGCCCGC TCTGGGGGGA TGCCACTTGC CTCCAGCTGG 
     3001 CCATGCAAGC TGACGCCCGT GCCTTCTTTG CCCAGGATGG GGTACAGTCT CTGCTGACAC 
     3061 AGAAGTGGTG GGGAGATATG GCCAGCACTA CACCCATCTG GGCCCTGGTT CTCGCCTTCT 
     3121 TTTGCCCTCC ACTCATCTAC ACCCGCCTCA TCACCTTCAG GAAATCAGAA GAGGAGCCCA 
     3181 CACGGGAGGA GCTAGAGTTT GACATGGATA GTGTCATTAA TGGGGAAGGG CCTGTCGGGA 
     3241 CGGCGGACCC AGCCGAGAAG ACGCCGCTGG GGGTCCCGCG CCAGTCGGGC CGTCCGGGTT 
     3301 GCTGCGGGGG CCGCTGCGGG GGGCGCCGGT GCCTACGCCG CTGGTTCCAC TTCTGGGGCG 
     3361 CGCCGGTGAC CATCTTCATG GGCAACGTGG TCAGCTACCT GCTGTTCCTG CTGCTTTTCT 
     3421 CGCGGGTGCT GCTCGTGGAT TTCCAGCCGG CGCCGCCCGG CTCCCTGGAG CTGCTGCTCT 
     3481 ATTTCTGGGC TTTCACGCTG CTGTGCGAGG AACTGCGCCA GGGCCTGAGC GGAGGCGGGG 
     3541 GCAGCCTCGC CAGCGGGGGC CCCGGGCCTG GCCATGCCTC ACTGAGCCAG CGCCTGCGCC 
     3601 TCTACCTCGC CGACAGCTGG AACCAGTGCG ACCTAGTGGC TCTCACCTGC TTCCTCCTGG 
     3661 GCGTGGGCTG CCGGCTGACC CCGGGTTTGT ACCACCTGGG CCGCACTGTC CTCTGCATCG 
     3721 ACTTCATGGT TTTCACGGTG CGGCTGCTTC ACATCTTCAC GGTCAACAAA CAGCTGGGGC 
     3781 CCAAGATCGT CATCGTGAGC AAGATGATGA AGGACGTGTT CTTCTTCCTC TTCTTCCTCG 
     3841 GCGTGTGGCT GGTAGCCTAT GGCGTGGCCA CGGAGGGGCT CCTGAGGCCA CGGGACAGTG 
     3901 ACTTCCCAAG TATCCTGCGC CGCGTCTTCT ACCGTCCCTA CCTGCAGATC TTCGGGCAGA 
     3961 TTCCCCAGGA GGACATGGAC GTGGCCCTCA TGGAGCACAG CAACTGCTCG TCGGAGCCCG 
     4021 GCTTCTGGGC ACACCCTCCT GGGGCCCAGG CGGGCACCTG CGTCTCCCAG TATGCCAACT 
     4081 GGCTGGTGGT GCTGCTCCTC GTCATCTTCC TGCTCGTGGC CAACATCCTG CTGGTCAACT 
     4141 TGCTCATTGC CATGTTCAGT TACACATTCG GCAAAGTACA GGGCAACAGC GATCTCTACT 
     4201 GGAAGGCGCA GCGTTACCGC CTCATCCGGG AATTCCACTC TCGGCCCGCG CTGGCCCCGC 
     4261 CCTTTATCGT CATCTCCCAC TTGCGCCTCC TGCTCAGGCA ATTGTGCAGG CGACCCCGGA 
     4321 GCCCCCAGCC GTCCTCCCCG GCCCTCGAGC ATTTCCGGGT TTACCTTTCT AAGGAAGCCG 
     4381 AGCGGAAGCT GCTAACGTGG GAATCGGTGC ATAAGGAGAA CTTTCTGCTG GCACGCGCTA 
     4441 GGGACAAGCG GGAGAGCGAC TCCGAGCGTC TGAAGCGCAC GTCCCAGAAG GTGGACTTGG 
     4501 CACTGAAACA GCTGGGACAC ATCCGCGAGT ACGAACAGCG CCTGAAAGTG CTGGAGCGGG 
     4561 AGGTCCAGCA GTGTAGCCGC GTCCTGGGGT GGGTGGCCGA GGCCCTGAGC CGCTCTGCCT 
     4621 TGCTGCCCCC AGGTGGGCCG CCACCCCCTG ACCTGCCTGG GTCCAAAGAC ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//