LOCUS       RC216867                7695 bp    DNA     circular  SYN 
DEFINITION  ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2)
ACCESSION   NM_015114
VERSION     NM_015114.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3852..3943 
                     /label="Myc-DDK"  
     misc_feature    3973..4558 
                     /label="PolyA signal"  
     misc_feature    4030..4049 
                     /label="Restriction Enzyme"  
     misc_feature    4834..5506 
                     /label="ColE1"  
     misc_feature    5936..6730 
                     /label="Kan/Neo"  
     misc_feature    6765..7067 
                     /label="SV40 ori"  
     misc_feature    7153..7630 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3834..3840 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3834 
                     /label="ANKLE2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTGGCCG CGGCTGGCGG CGGCCGAGTG GGCGGCGCTG GCCTGGGAGC 
     1081 TGCTGGGCGC CTCGGTGCTG CTGATCGCTG TGCGGTGGCT GGTGCGGCGG CTGGGCCCGC 
     1141 GGCCGGGAGG TCTGGGCCGC AGCGGGACCC CAGTTCCTCC GCCAAGCGCG GCCGCCGCCC 
     1201 CCGCCTCAGG TGAAATGACA ATGGATGCTC TGTTGGCTCG ATTGAAACTT CTGAATCCAG 
     1261 ATGACCTTAG AGAAGAAATC GTCAAAGCCG GATTGAAATG TGGACCCATT ACATCAACTA 
     1321 CAAGGTTCAT TTTTGAGAAA AAATTGGCTC AGGCTTTACT GGAGCAAGGA GGAAGGCTGT 
     1381 CTTCTTTCTA CCACCATGAG GCAGGTGTCA CAGCTCTCAG CCAGGACCCA CAAAGGATTT 
     1441 TGAAGCCAGC TGAAGGGAAC CCAACTGATC AGGCTGGTTT TTCTGAAGAC AGAGATTTTG 
     1501 GTTACAGTGT GGGCCTGAAT CCTCCAGAGG AGGAAGCTGT GACATCCAAG ACCTGCTCGG 
     1561 TGCCCCCTAG TGACACCGAC ACCTACAGAG CTGGAGCGAC TGCGTCTAAG GAGCCGCCCC 
     1621 TGTACTATGG GGTGTGTCCA GTGTATGAGG ACGTCCCAGC GAGAAATGAA AGGATCTATG 
     1681 TTTATGAAAA TAAAAAGGAA GCATTGCAAG CTGTCAAGAT GATCAAAGGG TCCCGATTTA 
     1741 AAGCTTTTTC TACCAGAGAA GACGCTGAGA AATTTGCTAG AGGAATTTGT GATTATTTCC 
     1801 CTTCTCCAAG CAAAACGTCC TTACCACTGT CTCCTGTGAA AACAGCTCCA CTCTTTAGCA 
     1861 ATGACAGGTT GAAAGATGGT TTGTGCTTGT CGGAATCAGA AACAGTCAAC AAAGAGCGAG 
     1921 CGAACAGTTA CAAAAATCCC CGCACGCAGG ACCTCACCGC CAAGCTTCGG AAAGCTGTGG 
     1981 AGAAGGGAGA GGAGGACACC TTTTCTGACC TTATCTGGAG CAACCCCCGG TATCTGATAG 
     2041 GCTCAGGAGA CAACCCCACT ATCGTGCAGG AAGGGTGCAG GTACAACGTG ATGCATGTTG 
     2101 CTGCCAAAGA GAACCAGGCT TCCATCTGCC AGCTGACTCT GGACGTCCTG GAGAACCCTG 
     2161 ACTTCATGAG GCTGATGTAC CCTGATGACG ACGAGGCCAT GCTGCAGAAG CGTATCCGTT 
     2221 ACGTGGTGGA CCTGTACCTC AACACCCCCG ACAAGATGGG CTATGACACA CCGTTGCATT 
     2281 TTGCTTGTAA GTTTGGAAAT GCAGATGTAG TCAACGTGCT TTCGTCACAC CATTTGATTG 
     2341 TAAAAAACTC AAGGAATAAA TATGATAAAA CACCTGAAGA TGTAATTTGT GAAAGAAGCA 
     2401 AAAATAAATC TGTGGAACTG AAGGAGCGGA TCAGAGAGTA TTTAAAGGGC CACTACTACG 
     2461 TGCCCCTCCT GAGAGCGGAA GAGACTTCTT CTCCAGTCAT CGGGGAGCTG TGGTCCCCAG 
     2521 ACCAGACGGC TGAGGCCTCT CACGTCAGCC GCTATGGAGG CAGCCCCAGA GACCCGGTAC 
     2581 TGACCCTGAG AGCCTTCGCA GGGCCCCTGA GTCCAGCCAA GGCAGAAGAT TTTCGCAAGC 
     2641 TCTGGAAAAC TCCACCTCGA GAGAAAGCAG GCTTCCTTCA CCACGTCAAG AAGTCGGACC 
     2701 CGGAAAGAGG CTTTGAGAGA GTGGGAAGGG AGCTAGCTCA TGAGCTGGGG TATCCCTGGG 
     2761 TTGAATACTG GGAATTTCTG GGCTGTTTTG TTGATCTGTC TTCCCAGGAA GGCCTGCAAA 
     2821 GACTAGAAGA ATATCTCACA CAGCAGGAAA TAGGCAAAAA GGCTCAACAA GAAACAGGAG 
     2881 AACGGGAAGC CTCCTGCCGA GATAAAGCCA CCACGTCTGG CAGCAATTCC ATTTCCGTGA 
     2941 GGGCGTTTCT AGATGAAGAT GACATGAGCT TGGAAGAAAT AAAAAATCGG CAAAATGCAG 
     3001 CTCGAAATAA CAGCCCGCCC ACAGTCGGTG CTTTTGGACA TACGAGGTGC AGCGCCTTCC 
     3061 CCTTGGAGCA GGAGGCAGAC CTCATAGAAG CCGCCGAGCC GGGAGGTCCA CACAGCAGCA 
     3121 GAAATGGGCT CTGCCATCCT CTGAATCACA GCAGGACCCT GGCGGGCAAG AGACCAAAGG 
     3181 CCCCCCGTGG GGAGGAAGCC CATCTGCCAC CTGTCTCGGA TTTGACTGTT GAGTTTGATA 
     3241 AACTGAATTT GCAAAATATA GGACGTAGCG TTTCCAAGAC ACCAGATGAA AGTACAAAAA 
     3301 CTAAAGATCA GATCCTGACT TCAAGAATCA ATGCAGTAGA AAGAGACTTG TTAGAGCCTT 
     3361 CTCCCGCAGA CCAACTCGGG AATGGCCACA GGAGGACAGA AAGTGAAATG TCAGCCAGGA 
     3421 TCGCTAAAAT GTCCTTGAGT CCCAGCAGCC CCAGGCACGA GGATCAGCTC GAGGTCACCA 
     3481 GGGAACCGGC CAGGCGGCTC TTCCTTTTTG GAGAGGAGCC ATCAAAACTC GATCAGGATG 
     3541 TTTTGGCCGC TCTTGAATGT GCAGACGTCG ACCCCCATCA GTTCCCGGCC GTGCACAGAT 
     3601 GGAAGAGTGC TGTCCTGTGC TACTCACCCT CGGACAGACA GAGTTGGCCC AGTCCCGCGG 
     3661 TGAAAGGAAG GTTCAAGTCT CAGCTGCCAG ATCTCAGTGG CCCTCACAGC TACAGTCCGG 
     3721 GGAGAAACAG CGTGGCTGGA AGCAACCCCG CAAAGCCAGG CCTGGGCAGT CCTGGGCGCT 
     3781 ACAGCCCCGT GCACGGGAGC CAGCTCCGCA GGATGGCGCG CCTGGCTGAG CTTGCCGCCC 
     3841 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3901 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3961 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4021 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4081 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4141 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4201 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4261 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4321 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4381 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4441 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4501 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4561 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4621 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4681 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4741 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4801 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4861 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4921 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4981 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5041 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5101 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5161 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5221 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5281 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5341 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5401 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5461 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5521 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5581 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5641 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5701 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5761 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5821 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5881 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5941 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6001 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6061 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6121 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6181 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6241 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6301 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6361 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6421 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6481 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6541 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6601 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6661 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6721 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6781 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6841 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6901 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7021 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7081 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7141 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7201 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7261 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7321 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7381 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7441 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7501 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7561 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7621 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7681 TTTAACGCGA ATTTT
//