LOCUS       RC216859                8367 bp    DNA     circular  SYN 
DEFINITION  SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B)
ACCESSION   NM_015260
VERSION     NM_015260.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4524..4615 
                     /label="Myc-DDK"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    4702..4721 
                     /label="Restriction Enzyme"  
     misc_feature    5506..6178 
                     /label="ColE1"  
     misc_feature    6608..7402 
                     /label="Kan/Neo"  
     misc_feature    7437..7739 
                     /label="SV40 ori"  
     misc_feature    7825..8302 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4506..4512 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4506 
                     /label="SIN3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCACGCT GGCGGTGGCA GCGGTGGCAG CGGTGCCGGC GGCCCCGCGG 
     1081 GCCGGGGGCT GAGCGGCGCC CGCTGGGGTC GCTCGGGCTC CGCAGGCCAC GAGAAGCTGC 
     1141 CGGTGCACGT AGAAGACGCC CTCACCTATC TGGACCAGGT GAAGATCCGC TTTGGCAGCG 
     1201 ACCCTGCCAC CTACAACGGC TTCCTGGAGA TCATGAAGGA GTTCAAAAGC CAGAGCATCG 
     1261 ATACTCCTGG AGTCATCAGA CGTGTCTCGC AGCTCTTCCA CGAGCACCCT GACCTCATTG 
     1321 TTGGATTCAA CGCTTTTCTT CCCCTCGGAT ATAGAATAGA CATTCCCAAG AATGGCAAGT 
     1381 TAAACATACA GTCGCCTCTG ACAAGCCAGG AGAATTCGCA CAACCACGGG GACGGTGCAG 
     1441 AGGACTTCAA GCAGCAGGTG CCGTATAAAG AGGACAAACC CCAGGTGCCC CTGGAGTCCG 
     1501 ATTCCGTGGA ATTCAACAAC GCCATCAGCT ATGTGAATAA GATTAAAACC CGCTTCCTAG 
     1561 ACCACCCAGA AATCTACAGG TCATTCCTGG AGATCCTGCA CACGTACCAG AAGGAGCAGC 
     1621 TGAACACGAG GGGCCGGCCA TTCCGAGGCA TGTCTGAAGA GGAGGTGTTC ACCGAGGTGG 
     1681 CCAACCTCTT CCGGGGCCAG GAGGACCTGC TCTCAGAGTT TGGACAGTTC CTGCCCGAAG 
     1741 CCAAGCGGTC TCTGTTCACA GGAAACGGGC CGTGCGAGAT GCACAGCGTG CAGAAGAACG 
     1801 AGCACGACAA GACCCCGGAG CACAGCAGGA AGCGCTCCCG GCCCTCGCTC CTCCGCCCCG 
     1861 TGTCTGCACC CGCCAAGAAA AAAATGAAAC TTCGTGGTAC CAAAGACCTG TCCATCGCTG 
     1921 CAGTGGGGAA GTACGGGACT CTGCAGGAAT TTTCTTTCTT TGACAAGGTC CGCCGGGTGC 
     1981 TGAAGAGCCA GGAGGTGTAT GAAAACTTCC TCCGCTGCAT CGCACTCTTC AACCAGGAGC 
     2041 TGGTGTCTGG CTCTGAGCTC CTGCAGCTCG TCAGCCCATT TCTGGGGAAA TTTCCAGAAC 
     2101 TCTTTGCACA GTTCAAGTCC TTCCTGGGGG TAAAAGAGCT GTCCTTCGCG CCACCCATGA 
     2161 GCGACAGATC CGGGGACGGG ATAAGCCGGG AAATTGATTA TGCATCCTGC AAGCGCATAG 
     2221 GATCCAGCTA CCGGGCACTC CCCAAAACCT ACCAGCAGCC CAAGTGCAGT GGGAGGACAG 
     2281 CCATCTGCAA GGAGCTTGAC CATTGGACAC TTCTCCAGGG TTCGTGGACA GACGATTACT 
     2341 GCATGTCCAA GTTCAAGAAT ACCTGCTGGA TTCCAGGATA TAGTGCAGGG GTACTGAACG 
     2401 ACACCTGGGT CTCCTTCCCT TCCTGGTCTG AGGACTCCAC GTTCGTCAGC TCCAAGAAGA 
     2461 CACCGTACGA GGAGCAGCTT CACCGCTGTG AGGACGAGCG CTTCGAGTTA GACGTTGTCC 
     2521 TGGAGACGAA CCTGGCCACA ATCCGTGTGT TGGAAAGTGT GCAGAAGAAG CTGTCTCGGA 
     2581 TGGCGCCGGA AGACCAGGAG AAGTTCCGGC TGGACGACTC CCTGGGAGGC ACGTCGGAGG 
     2641 TGATCCAGCG CCGTGCCATT TATCGCATCT ATGGCGACAA GGCCCCGGAG ATCATCGAGA 
     2701 GCCTCAAGAA GAACCCTGTC ACCGCTGTCC CCGTTGTCCT GAAAAGACTG AAGGCCAAGG 
     2761 AAGAGGAGTG GCGGGAGGCC CAGCAGGGCT TCAACAAGAT CTGGCGGGAG CAGTATGAGA 
     2821 AGGCGTACCT CAAGTCCCTT GACCACCAGG CTGTGAACTT CAAGCAGAAC GACACCAAGG 
     2881 CCCTGCGCTC CAAGAGCTTG CTCAACGAGA TCGAGAGCGT CTACGACGAG CACCAGGAGC 
     2941 AGCACTCGGA GGGCCGCAGT GCCCCCTCTA GCGAGCCGCA CCTCATCTTT GTGTACGAGG 
     3001 ACCGGCAGAT CCTGGAGGAC GCAGCAGCGC TCATCAGCTA CTACGTGAAG CGGCAGCCGG 
     3061 CCATCCAGAA GGAGGACCAG GGCACCATCC ACCAGCTGCT GCACCAGTTC GTGCCCAGCC 
     3121 TCTTCTTCTC TCAGCAGCTG GACCTGGGCG CCTCCGAGGA GTCAGCTGAT GAGGACCGGG 
     3181 ACAGCCCCCA GGGGCAGACC ACAGACCCCA GTGAGCGGAA GAAGCCGGCG CCAGGACCCC 
     3241 ACAGTAGCCC CCCAGAGGAG AAGGGGGCCT TCGGGGATGC CCCGGCCACT GAGCAGCCAC 
     3301 CCCTGCCGCC CCCAGCCCCG CACAAGCCCC TGGACGATGT CTACAGCCTA TTTTTTGCCA 
     3361 ACAACAACTG GTACTTCTTC CTGCGCCTGC ACCAGACCCT GTGCTCCAGG CTGCTGAAGA 
     3421 TCTACCGCCA GGCGCAGAAG CAGCTTCTGG AGTATCGGAC CGAGAAGGAG CGGGAGAAGC 
     3481 TGCTGTGTGA GGGCCGCAGG GAGAAGGGCA GCGACCCCGC CATGGAGCTG CGGCTGAAGC 
     3541 AGCCCAGTGA AGTGGAGCTG GAGGAGTACT ACCCGGCCTT CCTGGACATG GTGCGGAGCC 
     3601 TGCTGGAGGG CAGCATCGAC CCCACGCAGT ACGAGGACAC CCTACGCGAG ATGTTCACCA 
     3661 TCCATGCCTA CGTGGGCTTC ACCATGGACA AGCTGGTGCA GAACATTGCG CGGCAGCTGC 
     3721 ACCACCTCGT GAGCGATGAC GTCTGCCTGA AAGTGGTGGA GCTCTACCTG AACGAGAAGA 
     3781 AGCGGGGTGC CGCTGGTGGG AACCTGTCCT CCCGCTGCGT CCGCGCTGCT AGGGAGACCA 
     3841 GCTACCAGTG GAAGGCTGAG CGCTGCATGG CCGACGAGAA CTGCTTCAAG GTGATGTTCC 
     3901 TGCAGCGCAA AGGGCAGGTG ATCATGACCA TCGAGCTCCT GGACACCGAG GAGGCCCAGA 
     3961 CGGAGGACCC TGTGGAGGTC CAGCACCTGG CTCGGTACGT GGAGCAGTAT GTGGGGACCG 
     4021 AGGGCGCGTC CAGCTCGCCC ACTGAGGGCT TCCTCCTGAA ACCTGTGTTC CTGCAGAGGA 
     4081 ACCTCAAGAA GTTCCGCCGC CGGTGGCAGA GCGAGCAGGC GCGGGCCCTG CGCGGTGAGG 
     4141 CCAGGAGCTC CTGGAAGCGG CTGGTGGGCG TGGAGAGCGC CTGCGACGTG GACTGCCGCT 
     4201 TCAAGCTCAG CACTCACAAG ATGGTGTTCA TCGTGAACTC CGAGGACTAC ATGTACCGTC 
     4261 GCGGGACCCT CTGCCGGGCC AAGCAGGTGC AGCCCCTGGT CCTGCTCCGC CACCACCAGC 
     4321 ACTTTGAGGA GTGGCACAGC CGCTGGCTGG AGGACAATGT GACGGTGGAG GCGGCTAGCC 
     4381 TGGTGCAGGA CTGGCTGATG GGTGAGGAGG ACGAGGACAT GGTACCCTGC AAGACGCTGT 
     4441 GTGAGACAGT GCACGTGCAC GGCCTGCCCG TGACCCGCTA CCGCGTGCAG TACAGCCGCC 
     4501 GCCCGGCCTC GCCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4561 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5341 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5401 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5461 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5521 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5581 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5641 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5701 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5761 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5821 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5881 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5941 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6001 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6061 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6121 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6181 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6241 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6301 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6361 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6421 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6481 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6541 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6601 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6661 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6721 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6781 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6841 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6901 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6961 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7021 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7081 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7141 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7201 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7261 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7321 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7381 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7441 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7501 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7561 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7801 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7921 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8281 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8341 ATTTAACAAA AATTTAACGC GAATTTT
//