LOCUS       RC216819                8604 bp    DNA     circular  SYN 
DEFINITION  NAIP (Myc-DDK-tagged)-Human NLR family, apoptosis inhibitory protein (NAIP), transcript variant 2
ACCESSION   NM_022892
VERSION     NM_022892.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4761..4852 
                     /label="Myc-DDK"  
     misc_feature    4882..5467 
                     /label="PolyA signal"  
     misc_feature    4939..4958 
                     /label="Restriction Enzyme"  
     misc_feature    5743..6415 
                     /label="ColE1"  
     misc_feature    6845..7639 
                     /label="Kan/Neo"  
     misc_feature    7674..7976 
                     /label="SV40 ori"  
     misc_feature    8062..8539 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4743..4749 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4743 
                     /label="NAIP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTTCAT ATTGGGGACT TCGTCTGGGA TTCCAAGGTG CATTCATTGC 
     1081 AAAGTTCCTT AAATATTTTC TCACTGCTTC CTACTAAAGG ACGGACAGAG CATTTGTTCT 
     1141 TCAGCCACAT ACTTTCCTTC CACTGGCCAG CATTCTCCTC TATTAGACTA GAACTGTGGA 
     1201 TAAACCTCAG ATGTGAATTT CTTCGGAGTA AGAAATCCTC AGAGGAAATT ACCCAGTATA 
     1261 TTCAAAGCTA CAAGGGATTT GTTGACATAA CGGGAGAACA TTTTGTGAAT TCCTGGGTCC 
     1321 AGAGAGAATT ACCTATGGCA TCAGCTTATT GCAATGACAG CATCTTTGCT TACGAAGAAC 
     1381 TACGGCTGGA CTCTTTTAAG GACTGGCCCC GGGAATCAGC TGTGGGAGTT GCAGCACTGG 
     1441 CCAAAGCAGG TCTTTTCTAC ACAGGTATAA AGGACATCGT CCAGTGCTTT TCCTGTGGAG 
     1501 GGTGTTTAGA GAAATGGCAG GAAGGTGATG ACCCATTAGA CGATCACACC AGATGTTTTC 
     1561 CCAATTGTCC ATTTCTCCAA AATATGAAGT CCTCTGCGGA AGTGACTCCA GACCTTCAGA 
     1621 GCCGTGGTGA ACTTTGTGAA TTACTGGAAA CCACAAGTGA AAGCAATCTT GAAGATTCAA 
     1681 TAGCAGTTGG TCCTATAGTG CCAGAAATGG CACAGGGTGA AGCCCAGTGG TTTCAAGAGG 
     1741 CAAAGAATCT GAATGAGCAG CTGAGAGCAG CTTATACCAG CGCCAGTTTC CGCCACATGT 
     1801 CTTTGCTTGA TATCTCTTCC GATCTGGCCA CGGACCACTT GCTGGGCTGT GATCTGTCTA 
     1861 TTGCTTCAAA ACACATCAGC AAACCTGTGC AAGAACCTCT GGTGCTGCCT GAGGTCTTTG 
     1921 GCAACTTGAA CTCTGTCATG TGTGTGGAGG GTGAAGCTGG AAGTGGAAAG ACGGTCCTCC 
     1981 TGAAGAAAAT AGCTTTTCTG TGGGCATCTG GATGCTGTCC CCTGTTAAAC AGGTTCCAGC 
     2041 TGGTTTTCTA CCTCTCCCTT AGTTCCACCA GACCAGACGA GGGGCTGGCC AGTATCATCT 
     2101 GTGACCAGCT CCTAGAGAAA GAAGGATCTG TTACTGAAAT GTGCGTGAGG AACATTATCC 
     2161 AGCAGTTAAA GAATCAGGTC TTATTCCTTT TAGATGACTA CAAAGAAATA TGTTCAATCC 
     2221 CTCAAGTCAT AGGAAAACTG ATTCAAAAAA ACCACTTATC CCGGACCTGC CTATTGATTG 
     2281 CTGTCCGTAC AAACAGGGCC AGGGACATCC GCCGATACCT AGAGACCATT CTAGAGATCA 
     2341 AAGCATTTCC CTTTTATAAT ACTGTCTGTA TATTACGGAA GCTCTTTTCA CATAATATGA 
     2401 CTCGTCTGCG AAAGTTTATG GTTTACTTTG GAAAGAACCA AAGTTTGCAG AAGATACAGA 
     2461 AAACTCCTCT CTTTGTGGCG GCGATCTGTG CTCATTGGTT TCAGTATCCT TTTGACCCAT 
     2521 CCTTTGATGA TGTGGCTGTT TTCAAGTCCT ATATGGAACG CCTTTCCTTA AGGAACAAAG 
     2581 CGACAGCTGA AATTCTCAAA GCAACTGTGT CCTCCTGTGG TGAGCTGGCC TTGAAAGGGT 
     2641 TTTTTTCATG TTGCTTTGAG TTTAATGATG ATGATCTCGC AGAAGCAGGG GTTGATGAAG 
     2701 ATGAAGATCT AACCATGTGC TTGATGAGCA AATTTACAGC CCAGAGACTA AGACCATTCT 
     2761 ACCGGTTTTT AAGTCCTGCC TTCCAAGAAT TTCTTGCGGG GATGAGGCTG ATTGAACTCC 
     2821 TGGATTCAGA TAGGCAGGAA CATCAAGATT TGGGACTGTA TCATTTGAAA CAAATCAACT 
     2881 CACCCATGAT GACTGTAAGC GCCTACAACA ATTTTTTGAA CTATGTCTCC AGCCTCCCTT 
     2941 CAACAAAAGC AGGGCCCAAA ATTGTGTCTC ATTTGCTCCA TTTAGTGGAT AACAAAGAGT 
     3001 CATTGGAGAA TATATCTGAA AATGATGACT ACTTAAAGCA CCAGCCAGAA ATTTCACTGC 
     3061 AGATGCAGTT ACTTAGGGGA TTGTGGCAAA TTTGTCCACA AGCTTACTTT TCAATGGTTT 
     3121 CAGAACATTT ACTGGTTCTT GCCCTGAAAA CTGCTTATCA AAGCAACACT GTTGCTGCGT 
     3181 GTTCTCCATT TGTTTTGCAA TTCCTTCAAG GGAGAACACT GACTTTGGGT GCGCTTAACT 
     3241 TACAGTACTT TTTCGACCAC CCAGAAAGCT TGTCATTGTT GAGGAGCATC CACTTCCCAA 
     3301 TACGAGGAAA TAAGACATCA CCCAGAGCAC ATTTTTCAGT TCTGGAAACA TGTTTTGACA 
     3361 AATCACAGGT GCCAACTATA GATCAGGACT ATGCTTCTGC CTTTGAACCT ATGAATGAAT 
     3421 GGGAGCGAAA TTTAGCTGAA AAAGAGGATA ATGTAAAGAG CTATATGGAT ATGCAGCGCA 
     3481 GGGCATCACC AGACCTTAGT ACTGGCTATT GGAAACTTTC TCCAAAGCAG TACAAGATTC 
     3541 CCTGTCTAGA AGTCGATGTG AATGATATTG ATGTTGTAGG CCAGGATATG CTTGAGATTC 
     3601 TAATGACAGT TTTCTCAGCT TCACAGCGCA TCGAACTCCA TTTAAACCAC AGCAGAGGCT 
     3661 TTATAGAAAG CATCCGCCCA GCTCTTGAGC TGTCTAAGGC CTCTGTCACC AAGTGCTCCA 
     3721 TAAGCAAGTT GGAACTCAGC GCAGCCGAAC AGGAACTGCT TCTCACCCTG CCTTCCCTGG 
     3781 AATCTCTTGA AGTCTCAGGG ACAATCCAGT CACAAGACCA AATCTTTCCT AATCTGGATA 
     3841 AGTTCCTGTG CCTGAAAGAA CTGTCTGTGG ATCTGGAGGG CAATATAAAT GTTTTTTCAG 
     3901 TCATTCCTGA AGAATTTCCA AACTTCCACC ATATGGAGAA ATTATTGATC CAAATTTCAG 
     3961 CTGAGTATGA TCCTTCCAAA CTAGTAAAAT TAATTCAAAA TTCTCCAAAC CTTCATGTTT 
     4021 TCCATCTGAA GTGTAACTTC TTTTCGGATT TTGGGTCTCT CATGACTATG CTTGTTTCCT 
     4081 GTAAGAAACT CACAGAAATT AAGTTTTCGG ATTCATTTTT TCAAGCCGTC CCATTTGTTG 
     4141 CCAGTTTGCC AAATTTTATT TCTCTGAAGA TATTAAATCT TGAAGGCCAG CAATTTCCTG 
     4201 ATGAGGAAAC ATCAGAAAAA TTTGCCTACA TTTTAGGTTC TCTTAGTAAC CTGGAAGAAT 
     4261 TGATCCTTCC TACTGGGGAT GGAATTTATC GAGTGGCCAA ACTGATCATC CAGCAGTGTC 
     4321 AGCAGCTTCA TTGTCTCCGA GTCCTCTCAT TTTTCAAGAC TTTGAATGAT GACAGCGTGG 
     4381 TGGAAATTGC CAAAGTAGCA ATCAGTGGAG GTTTCCAGAA ACTTGAGAAC CTAAAGCTTT 
     4441 CAATCAATCA CAAGATTACA GAGGAAGGAT ACAGAAATTT CTTTCAAGCA CTGGACAACA 
     4501 TGCCAAACTT GCAGGAGTTG GACATCTCCA GGCATTTCAC AGAGTGTATC AAAGCTCAGG 
     4561 CCACAACAGT CAAGTCTTTG AGTCAATGTG TGTTACGACT ACCAAGGCTC ATTAGACTGA 
     4621 ACATGTTAAG TTGGCTCTTG GATGCAGATG ATATTGCATT GCTTAATGTC ATGAAAGAAA 
     4681 GACATCCTCA ATCTAAGTAC TTAACTATTC TCCAGAAATG GATACTGCCG TTCTCTCCAA 
     4741 TCATTCAGAA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4801 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4861 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4921 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4981 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5041 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5101 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5161 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5221 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5281 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5341 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5401 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5461 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5521 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5581 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5641 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5701 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5761 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5821 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5881 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5941 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6001 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6061 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6121 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6181 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6241 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6301 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6361 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6421 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6481 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6541 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6601 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6661 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6721 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6781 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6841 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6901 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6961 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7021 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7081 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7141 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7201 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7261 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7321 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7381 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7441 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7501 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7561 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7621 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7681 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7741 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7801 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8041 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8101 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8161 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8221 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8281 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8341 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8401 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8461 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8521 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8581 TAACAAAAAT TTAACGCGAA TTTT
//