LOCUS       RC216784                8043 bp    DNA     circular  SYN 
DEFINITION  FARP2 (Myc-DDK-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2)
ACCESSION   NM_014808
VERSION     NM_014808.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4200..4291 
                     /label="Myc-DDK"  
     misc_feature    4321..4906 
                     /label="PolyA signal"  
     misc_feature    4378..4397 
                     /label="Restriction Enzyme"  
     misc_feature    5182..5854 
                     /label="ColE1"  
     misc_feature    6284..7078 
                     /label="Kan/Neo"  
     misc_feature    7113..7415 
                     /label="SV40 ori"  
     misc_feature    7501..7978 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4182..4188 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4182 
                     /label="FARP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGAGATA GAAGGAACAT ACAGAGTCCT GCAGACTGCA GGGATGCGCT 
     1081 TGGGTGCCCA GACCCCTGTG GGAGTTAGCA CCCTTGAGCC TGGGCAGACT CTCTTGCCCA 
     1141 GAATGCAAGA GAAGCACCTG CACCTCAGAG TAAAGCTGCT GGACAACACC ATGGAAATAT 
     1201 TTGACATTGA GCCTAAATGC GATGGCCAGG TATTACTGAC ACAAGTGTGG AAGCGTTTAA 
     1261 ACCTGGTAGA ATGTGACTAC TTCGGGATGG AGTTTCAAAA TACTCAGTCC TACTGGATTT 
     1321 GGCTTGAACC TATGAAACCC ATCATTAGGC AAATACGAAG GCCAAAGAAT GTGGTGCTTC 
     1381 GCCTAGCTGT AAAATTTTTT CCACCTGATC CTGGTCAGCT ACAAGAAGAA TATACAAGAT 
     1441 ACTTGTTTGC CTTGCAACTT AAGAGAGACC TGCTGGAAGA GCGTTTGACC TGTGCTGACA 
     1501 CCACAGCGGC CCTTCTCACG TCCCATCTCC TGCAGTCGGA AATAGGAGAT TACGATGAAA 
     1561 CGCTGGACCG AGAGCACCTC AAAGTGAACG AGTATTTGCC TGGCCAGCAG CACTGCCTTG 
     1621 AGAAGATACT AGAATTCCAT CAGAAGCACG TGGGCCAGAC ACCTGCTGAG TCGGATTTCC 
     1681 AGGTGCTCGA AATTGCTCGA AAGTTGGAAA TGTACGGCAT CAGATTTCAC ATGGCTTCTG 
     1741 ACAGGGAAGG AACCAAGATT CAACTGGCAG TTTCCCACAT GGGTGTACTC GTGTTCCAGG 
     1801 GCACCACCAA AATCAACACT TTCAACTGGT CCAAGGTCCG TAAACTAAGC TTCAAGAGGA 
     1861 AAAGATTTCT TATCAAACTT CATCCAGAGG TTCATGGACC TTACCAGGAC ACATTAGAAT 
     1921 TTTTGTTGGG TAGTAGAGAT GAATGTAAGA ACTTCTGGAA GATTTGTGTG GAGTATCACA 
     1981 CCTTTTTTAG ACTTTTGGAC CAACCTAAGC CAAAAGCAAA AGCCGTCTTC TTCAGCCGGG 
     2041 GCTCCTCCTT CAGATACAGT GGAAGAACTC AGAAACAACT AGTAGATTAT TTCAAAGACA 
     2101 GTGGAATGAA GAGAATTCCA TATGAAAGAA GGCACAGCAA GACCCACACG TCCGTTCGAG 
     2161 CTCTGACTGC AGACCTACCA AAACAGAGCA TCTCATTCCC CGAGGGATTG AGGACTCCTG 
     2221 CCTCCCCATC TTCAGCGAAT GCCTTTTACT CGCTCTCTCC CTCCACTCTG GTCCCCTCTG 
     2281 GCCTGCCAGA GTTTAAGGAC AGCAGCAGCT CCCTCACAGA TCCCCAGGTT TCCTACGTCA 
     2341 AGAGTCCAGC TGCAGAGAGG CGCAGTGGAG CAGTGGCTGG AGGCCCCGAC ACACCATCGG 
     2401 CCCAGCCCCT CGGGCCCCCC GCACTCCAGC CTGGTCCAGG CCTTTCCACG AAGAGTCCTC 
     2461 AGCCTTCTCC CTCCAGCCGG AAGAGCCCCC TGAGTCTGAG CCCTGCATTT CAGGTGCCTT 
     2521 TGGGCCCAGC TGAACAGGGC TCATCCCCAC TCCTGAGCCC TGTCCTCAGT GATGCTGGCG 
     2581 GAGCCGGGAT GGACTGCGAG GAGCCCAGAC ACAAGCGCGT GCCTGCAGAC GAGGCCTACT 
     2641 TCATAGTCAA AGAGATTCTC GCTACAGAAC GAACATACCT CAAGGATTTA GAAGTTATTA 
     2701 CCGTGTGGTT CCGCAGCGCA GTGGTGAAGG AGGACGCCAT GCCTGCGACT CTGATGACGC 
     2761 TGCTCTTCTC CAACATCGAT CCCATCTATG AGTTCCACAG AGGCTTCCTG CGCGAGGTGG 
     2821 AGCAGAGGCT GGCACTCTGG GAAGGGCCCT CCAAAGCCCA CACAAAAGGC AGTCATCAAC 
     2881 GAATCGGGGA CATCCTGCTC AGGAACATGC GCCAGTTAAA GGAGTTTACC AGCTACTTCC 
     2941 AAAGACATGA CGAGGTCCTA ACAGAACTGG AAAAGGCTAC CAAACGCTGT AAGAAGTTGG 
     3001 AGGCAGTGTA CAAGGAGTTT GAGCTGCAGA AGGTCTGCTA CTTGCCTCTC AACACGTTCC 
     3061 TGCTGAAGCC CATCCAGCGG CTGCTGCACT ACCGCCTGCT GCTGCGCCGC CTATGCGGAC 
     3121 ATTACAGCCC CGGGCACCAT GACTACGCTG ACTGCCATGA CGCCCTGAAA GCCATCACAG 
     3181 AGGTGACCAC CACACTACAG CACATTCTCA TCCGGCTGGA GAACCTGCAG AAGCTAACGG 
     3241 AGCTGCAGCG GGACCTGGTG GGCATAGAGA ACCTCATTGC TCCTGGCAGG GAGTTCATCC 
     3301 GTGAGGGCTG CCTTCACAAG CTCACCAAGA AGGGCCTGCA GCAGAGGATG TTTTTTCTGT 
     3361 TCTCAGATAT GTTGCTGTAC ACAAGCAAAG GAGTTGCAGG GACCAGCCAC TTCCGGATCC 
     3421 GGGGCCTCCT TCCCCTCCAA GGCATGCTGG TGGAAGAAAG TGATAACGAG TGGTCTGTTC 
     3481 CACACTGTTT CACCATCTAC GCGGCTCAGA AAACAATCGT GGTGGCAGCC AGCACTCGGC 
     3541 TGGAGAAAGA GAAGTGGATG CTGGACCTGA ACTCCGCGAT CCAAGCAGCC AAGAGTGGCG 
     3601 GTGACACGGC CCCTGCACTG CCAGGCCGCA CTGTGTGCAC TCGTCCCCCC AGATCCCCCA 
     3661 ACGAGGTATC TCTGGAGCAG GAGTCAGAAG ATGATGCTCG GGGTGTCCGC AGCTCCCTGG 
     3721 AGGGGCATGG CCAGCACCGG GCCAACACCA CAATGCACGT GTGCTGGTAC CGGAACACCA 
     3781 GCGTGTCCAG GGCAGACCAC AGTGCAGCTG TCGAGAACCA GCTTTCAGGA TATCTGCTAA 
     3841 GAAAGTTCAA AAACAGTCAT GGCTGGCAGA AGCTCTGGGT CGTCTTTACC AACTTCTGTT 
     3901 TGTTCTTCTA CAAAACTCAT CAGGATGACT ACCCACTGGC CAGCCTCCCG CTGCTGGGCT 
     3961 ACAGCGTGAG CATCCCCAGG GAGGCCGATG GCATACACAA AGACTATGTT TTCAAGCTCC 
     4021 AGTTCAAATC CCACGTCTAC TTCTTCCGGG CTGAGAGCAA GTACACATTT GAAAGGTGGA 
     4081 TGGAGGTGAT CCAGGGGGCC AGCAGCTCAG CCGGGAGGGC CCCAAGCATC GTGCAGGATG 
     4141 GCCCCCAACC CTCCTCAGGG CTGGAGGGGA TGGTCAGGGG GAAGGAGGAA ACGCGTACGC 
     4201 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4261 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4321 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4381 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4441 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4501 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4561 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4621 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4681 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4741 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4801 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4861 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4921 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4981 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5041 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5101 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5161 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5221 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5281 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5341 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5401 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5461 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5521 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5581 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5641 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5701 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5761 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5821 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5881 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5941 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6001 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6061 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6121 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6181 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6241 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6301 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6361 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6421 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6481 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6541 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6601 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6661 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6721 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6781 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6841 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6901 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6961 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7021 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7081 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7141 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7201 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7261 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7321 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7381 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7441 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7501 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7561 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7621 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7681 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7741 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7801 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7861 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7921 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7981 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8041 TTT
//