LOCUS       RC216783                9471 bp    DNA     circular  SYN 
DEFINITION  SYCP2 (Myc-DDK-tagged)-Human synaptonemal complex protein 2 (SYCP2)
ACCESSION   NM_014258
VERSION     NM_014258.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5628..5719 
                     /label="Myc-DDK"  
     misc_feature    5749..6334 
                     /label="PolyA signal"  
     misc_feature    5806..5825 
                     /label="Restriction Enzyme"  
     misc_feature    6610..7282 
                     /label="ColE1"  
     misc_feature    7712..8506 
                     /label="Kan/Neo"  
     misc_feature    8541..8843 
                     /label="SV40 ori"  
     misc_feature    8929..9406 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5610..5616 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5610 
                     /label="SYCP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAATAAGA CCAGATCTCC AGCAGTTGGA AAAATGCATT GATGATGCTT 
     1081 TAAGAAAAAA TGATTTCAAA CCTTTGAAAA CACTTTTGCA AATTGATATT TGTGAAGATG 
     1141 TGAAGATTAA ATGCAGCAAA CAGTTTTTCC ACAAGGTGGA CAACCTTATA TGCAGGGAAC 
     1201 TTAATAAAGA GGATATCCAC AATGTTTCAG CCATTTTGGT TTCTGTTGGA AGATGTGGCA 
     1261 AAAATATCAG TGTATTGGGG CAAGCTGGAC TTCTAACGAT GATAAAACAA GGACTAATAC 
     1321 AAAAGATGGT TGCCTGGTTT GAAAAATCCA AGGACATTAT TCAGAGTCAA GGAAATTCAA 
     1381 AAGATGAAGC TGTTCTAAAT ATGATAGAAG ACTTAGTTGA TCTTCTGCTG GTCATACATG 
     1441 ATGTCAGTGA TGAAGGTAAA AAACAAGTAG TGGAAAGTTT CGTACCTCGC ATTTGTTCCC 
     1501 TGGTTATTGA CTCAAGAGTG AATATTTGTA TTCAGCAAGA GATTATAAAA AAAATGAATG 
     1561 CTATGCTTGA CAAAATGCCT CAAGATGCCC GGAAAATACT CTCTAACCAA GAAATGTTAA 
     1621 TTCTCATGAG TAGTATGGGA GAAAGGATTT TAGATGCTGG AGATTATGAC TTACAGGTAG 
     1681 GCATTGTAGA AGCTTTGTGT AGAATGACCA CAGAAAAACA AAGACAAGAA CTGGCACATC 
     1741 AGTGGTTTTC AATGGATTTT ATTGCTAAGG CATTTAAAAG AATTAAGGAC TCTGAATTTG 
     1801 AAACAGATTG CAGGATATTT CTCAACCTTG TAAATGGCAT GCTTGGAGAC AAAAGAAGGG 
     1861 TCTTTACATT TCCTTGTTTA TCAGCATTTC TTGATAAATA TGAGCTGCAA ATACCATCAG 
     1921 ATGAAAAACT TGAGGAATTT TGGATTGATT TTAATCTTGG GAGTCAGACT CTCTCATTCT 
     1981 ACATTGCTGG AGATAATGAT GATCATCAAT GGGAAGCAGT TACTGTGCCA GAGGAAAAAG 
     2041 TACAAATATA CAGCATTGAA GTGAGAGAAT CAAAGAAGCT ACTGACAATA ATTCTGAAAA 
     2101 ATACAGTAAA AATTAGCAAA AGAGAAGGGA AAGAATTGCT TTTGTATTTT GACGCATCAC 
     2161 TAGAAATCAC TAATGTAACT CAAAAAATTT TTGGTGCAAC TAAACATAGG GAATCTATCA 
     2221 GAAAACAAGG TATTTCAGTT GCCAAAACGT CGCTGCATAT ACTTTTTGAC GCAAGTGGAT 
     2281 CACAGATTCT AGTGCCAGAA AGTCAAATCT CACCAGTCGG AGAAGAGCTC GTTAGTTTAA 
     2341 AGGAAAAATC AAAGTCCCCA AAGGAATTTG CTAAACCTTC AAAATATATC AAAAACAGTG 
     2401 ACAAAGGGAA TAGAAATAAT AGTCAGCTTG AGAAAACTAC TCCTAGCAAA AGAAAAATGT 
     2461 CTGAAGCATC AATGATTGTT TCTGGTGCAG ATAGATACAC TATGAGAAGT CCAGTGCTTT 
     2521 TCAGCAACAC ATCAATACCA CCACGAAGAA GAAGAATTAA ACCACCACTG CAAATGACGA 
     2581 GCTCTGCAGA GAAACCTAGT GTTTCTCAAA CATCAGAAAA TAGAGTGGAT AATGCTGCAT 
     2641 CACTGAAATC TAGATCATCA GAAGGAAGAC ATAGAAGAGA TAATATAGAC AAACATATCA 
     2701 AAACTGCTAA GTGTGTAGAA AACACAGAAA ATAAGAATGT TGAATTCCCA AACCAAAATT 
     2761 TTAGTGAACT CCAGGATGTT ATACCAGATT CACAGGCAGC GGAAAAAAGA GATCATACTA 
     2821 TATTACCTGG TGTTTTAGAC AACATCTGTG GAAATAAAAT ACACAGCAAA TGGGCATGTT 
     2881 GGACACCTGT AACAAACATT GAACTATGTA ATAACCAAAG AGCAAGTACT TCGTCAGGAG 
     2941 ACACATTGAA TCAAGATATT GTTATAAATA AAAAACTTAC TAAACAAAAA TCATCCTCTT 
     3001 CAATATCTGA TCATAATTCT GAAGGAACAG GAAAAGTGAA ATATAAGAAA GAACAAACCG 
     3061 ACCATATCAA AATAGATAAA GCAGAAGTAG AAGTTTGCAA GAAACACAAT CAGCAACAAA 
     3121 ATCATCCTAA ATATTCAGGG CAGAAAAATA CTGAAAATGC CAAGCAGAGT GATTGGCCTG 
     3181 TTGAATCTGA AACTACTTTT AAATCGGTTC TCCTAAATAA GACAATTGAA GAATCGCTGA 
     3241 TATATAGGAA GAAATACATA TTGTCAAAAG ATGTGAATAC TGCTACTTGC GATAAAAATC 
     3301 CATCTGCTAG CAAAAATGTG CAAAGTCATA GAAAAGCAGA GAAAGAATTG ACTTCTGAGC 
     3361 TTAATTCCTG GGATTCGAAA CAAAAAAAAA TGAGAGAAAA GTCAAAAGGG AAAGAATTTA 
     3421 CCAATGTAGC AGAATCCTTG ATAAGCCAAA TCAATAAAAG ATACAAAACA AAAGATGACA 
     3481 TCAAGTCTAC AAGAAAATTA AAGGAGTCTT TGATTAACAG TGGTTTTTCA AACAAACCTG 
     3541 TTGTACAACT CAGTAAGGAA AAAGTTCAGA AAAAAAGCTA CAGAAAACTG AAGACTACCT 
     3601 TTGTTAATGT TACTTCTGAA TGCCCAGTGA ATGATGTTTA CAATTTTAAT TTGAATGGAG 
     3661 CTGATGACCC TATCATAAAA CTTGGAATCC AAGAGTTTCA AGCTACAGCT AAAGAAGCTT 
     3721 GTGCGGATAG GTCAATTAGA TTGGTAGGTC CAAGGAATCA TGATGAACTT AAATCTTCTG 
     3781 TCAAAACAAA AGATAAAAAA ATTATAACAA ATCATCAAAA GAAAAATCTG TTTAGTGATA 
     3841 CTGAAACAGA GTACAGATGT GATGACAGCA AGACTGATAT TAGCTGGCTA AGAGAACCGA 
     3901 AATCAAAACC ACAGCTAATA GACTATAGCA GAAATAAAAA TGTGAAGAAT CATAAAAGTG 
     3961 GAAAATCAAG ATCATCCTTG GAAAAGGGAC AGCCAAGCTC TAAAATGACA CCCAGTAAAA 
     4021 ATATCACAAA AAAGATGGAC AAGACAATTC CGGAAGGAAG AATCAGACTT CCACGAAAAG 
     4081 CAACCAAAAC AAAAAAAAAC TATAAAGATC TCTCAAATTC AGAATCAGAG TGTGAACAAG 
     4141 AATTTTCACA TTCATTTAAA GAGAACATAC CAGTAAAGGA GGAGAATATC CATTCCAGAA 
     4201 TGAAAACGGT AAAGCTACCA AAGAAACAAC AGAAAGTCTT CTGTGCTGAA ACAGAAAAGG 
     4261 AACTATCAAA ACAATGGAAA AACTCATCTC TACTAAAAGA TGCTATACGA GATAATTGCC 
     4321 TTGACTTATC TCCCAGATCT TTATCTGGCA GTCCATCATC TATAGAAGTA ACGAGATGTA 
     4381 TAGAGAAAAT AACAGAAAAG GATTTTACTC AGGATTATGA CTGCATAACA AAATCTATAT 
     4441 CACCTTATCC AAAAACTTCA TCACTTGAAT CCTTAAATAG TAACAGTGGA GTTGGAGGTA 
     4501 CAATAAAGTC ACCCAAAAAC AATGAGAAAA ACTTCCTGTG TGCAAGTGAA AGTTGTTCAC 
     4561 CAATTCCACG ACCACTGTTT TTGCCCAGAC ATACTCCAAC TAAGAGTAAT ACTATTGTAA 
     4621 ATAGAAAAAA AATAAGTTCT CTGGTACTTA CACAAGAAAC ACAAAACAGT AACAGCTATT 
     4681 CAGATGTAAG CAGTTATAGT TCAGAAGAAC GGTTTATGGA AATTGAATCT CCACATATCA 
     4741 ATGAAAATTA TATACAAAGC AAAAGAGAGG AAAGTCATTT AGCATCTTCA TTATCCAAGT 
     4801 CTAGTGAAGG AAGAGAGAAA ACGTGGTTTG ACATGCCCTG TGATGCTACT CATGTATCAG 
     4861 GCCCCACCCA ACATCTTAGT CGCAAAAGAA TATATATAGA AGATAATCTA AGTAATTCCA 
     4921 ATGAAGTAGA AATGGAAGAG AAAGGAGAAA GGAGAGCAAA CTTGCTTCCC AAAAAACTGT 
     4981 GTAAAATTGA AGATGCAGAT CATCATATCC ACAAAATGTC TGAAAGTGTA TCTTCATTAT 
     5041 CAACAAATGA CTTTTCTATT CCTTGGGAGA CCTGGCAAAA TGAATTTGCA GGGATAGAGA 
     5101 TGACTTATGA GACTTACGAG AGGCTCAATT CAGAATTTAA GAGAAGGAAT AATATCCGAC 
     5161 ATAAAATGTT GAGTTATTTT ACTACGCAGT CTTGGAAAAC AGCTCAGCAA CATCTGAGAA 
     5221 CAATGAATCA TCAAAGTCAG GACTCTAGGA TTAAAAAACT TGATAAATTC CAATTCATTA 
     5281 TCATAGAGGA GCTGGAGAAT TTTGAAAAAG ATTCACAGTC TTTAAAAGAT TTGGAAAAGG 
     5341 AATTTGTGGA CTTTTGGGAA AAGATATTTC AGAAGTTCAG TGCATATCAA AAAAGCGAAC 
     5401 AACAGAGGCT TCATCTTTTG AAAACTTCAT TGGCTAAAAG TGTCTTCTGT AATACTGATA 
     5461 GTGAAGAAAC TGTTTTTACA TCCGAGATGT GTTTGATGAA AGAAGATATG AAAGTGCTGC 
     5521 AAGACAGGCT TCTTAAGGAC ATGCTAGAAG AGGAGCTTCT TAATGTACGC AGAGAACTGA 
     5581 TGTCAGTATT CATGTCTCAT GAAAGAAATG CTAATGTGAC GCGTACGCGG CCGCTCGAGC 
     5641 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5701 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5761 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5821 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5881 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5941 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     6001 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     6061 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     6121 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6181 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6241 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6301 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6361 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6421 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6481 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6541 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6601 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6661 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6721 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6781 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6841 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6901 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6961 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     7021 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     7081 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     7141 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7201 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7261 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7321 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7381 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7441 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7501 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7561 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7621 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7681 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7741 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7801 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7861 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7921 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7981 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     8041 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     8101 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     8161 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8221 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8281 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8341 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8401 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8461 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8521 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8581 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8641 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8701 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8761 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8821 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8881 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8941 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     9001 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     9061 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     9121 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9181 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9241 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9301 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9361 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9421 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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