LOCUS       RC216778L1                9264 bp    DNA     circular  SYN 
DEFINITION  KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B)
ACCESSION   NM_004521
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7555..7735 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8463..9122 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7894..8462 
                     /label="ColE1"  
     misc_feature    6430..6450 
                     /label="LR50 primer"  
     misc_feature    6227..6298 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6439..7030 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6209..6215 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6209 
                     /label="KIF5B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGACCTG GCCGAGTGCA ACATCAAAGT 
     3361 GATGTGTCGC TTCAGACCTC TCAACGAGTC TGAAGTGAAC CGCGGCGACA AGTACATCGC 
     3421 CAAGTTTCAG GGAGAAGACA CGGTCGTGAT CGCGTCCAAG CCTTATGCAT TTGATCGGGT 
     3481 GTTCCAGTCA AGCACATCTC AAGAGCAAGT GTATAATGAC TGTGCAAAGA AGATTGTTAA 
     3541 AGATGTACTT GAAGGATATA ATGGAACAAT ATTTGCATAT GGACAAACAT CCTCTGGGAA 
     3601 GACACACACA ATGGAGGGTA AACTTCATGA TCCAGAAGGC ATGGGAATTA TTCCAAGAAT 
     3661 AGTGCAAGAT ATTTTTAATT ATATTTACTC CATGGATGAA AATTTGGAAT TTCATATTAA 
     3721 GGTTTCATAT TTTGAAATAT ATTTGGATAA GATAAGGGAC CTGTTAGATG TTTCAAAGAC 
     3781 CAACCTTTCA GTTCATGAAG ACAAAAACCG AGTTCCCTAT GTAAAGGGGT GCACAGAGCG 
     3841 TTTTGTATGT AGTCCAGATG AAGTTATGGA TACCATAGAT GAAGGAAAAT CCAACAGACA 
     3901 TGTAGCAGTT ACAAATATGA ATGAACATAG CTCTAGGAGT CACAGTATAT TTCTTATTAA 
     3961 TGTCAAACAA GAGAACACAC AAACGGAACA AAAGCTGAGT GGAAAACTTT ATCTGGTTGA 
     4021 TTTAGCTGGT AGTGAAAAGG TTAGTAAAAC TGGAGCTGAA GGTGCTGTGC TGGATGAAGC 
     4081 TAAAAACATC AACAAGTCAC TTTCTGCTCT TGGAAATGTT ATTTCTGCTT TGGCTGAGGG 
     4141 TAGTACATAT GTTCCATATC GAGATAGTAA AATGACAAGA ATCCTTCAAG ATTCATTAGG 
     4201 TGGCAACTGT AGAACCACTA TTGTAATTTG CTGCTCTCCA TCATCATACA ATGAGTCTGA 
     4261 AACAAAATCT ACACTCTTAT TTGGCCAAAG GGCCAAAACA ATTAAGAACA CAGTTTGTGT 
     4321 CAATGTGGAG TTAACTGCAG AACAGTGGAA AAAGAAGTAT GAAAAAGAAA AAGAAAAAAA 
     4381 TAAGATCCTG CGGAACACTA TTCAGTGGCT TGAAAATGAG CTCAACAGAT GGCGTAATGG 
     4441 GGAGACGGTG CCTATTGATG AACAGTTTGA CAAAGAGAAA GCCAACTTGG AAGCTTTCAC 
     4501 AGTGGATAAA GATATTACTC TTACCAATGA TAAACCAGCA ACCGCAATTG GAGTTATAGG 
     4561 AAATTTTACT GATGCTGAAA GAAGAAAGTG TGAAGAAGAA ATTGCTAAAT TATACAAACA 
     4621 GCTTGATGAC AAGGATGAAG AAATTAACCA GCAAAGTCAA CTGGTAGAGA AACTGAAGAC 
     4681 GCAAATGTTG GATCAGGAGG AGCTTTTGGC ATCTACCAGA AGGGATCAAG ACAATATGCA 
     4741 AGCTGAGCTG AATCGCCTTC AAGCAGAAAA TGATGCCTCT AAAGAAGAAG TGAAAGAAGT 
     4801 TTTACAGGCC CTAGAAGAAC TTGCTGTCAA TTATGATCAG AAGTCTCAGG AAGTTGAAGA 
     4861 CAAAACTAAG GAATATGAAT TGCTTAGTGA TGAATTGAAT CAGAAATCGG CAACTTTAGC 
     4921 GAGTATAGAT GCTGAGCTTC AGAAACTTAA GGAAATGACC AACCACCAGA AAAAACGAGC 
     4981 AGCTGAGATG ATGGCATCTT TACTAAAAGA CCTTGCAGAA ATAGGAATTG CTGTGGGAAA 
     5041 TAATGATGTA AAGCAGCCTG AGGGAACTGG CATGATAGAT GAAGAGTTCA CTGTTGCAAG 
     5101 ACTCTACATT AGCAAAATGA AGTCAGAAGT AAAAACCATG GTGAAACGTT GCAAGCAGTT 
     5161 AGAAAGCACA CAAACTGAGA GCAACAAAAA AATGGAAGAA AATGAAAAGG AGTTAGCAGC 
     5221 ATGTCAGCTT CGTATCTCTC AACATGAAGC CAAAATCAAG TCATTGACTG AATACCTTCA 
     5281 AAATGTGGAA CAAAAGAAAA GACAGTTGGA GGAATCTGTC GATGCCCTCA GTGAAGAACT 
     5341 AGTCCAGCTT CGAGCACAAG AGAAAGTCCA TGAAATGGAA AAGGAGCACT TAAATAAGGT 
     5401 TCAGACTGCA AATGAAGTTA AGCAAGCTGT TGAACAGCAG ATCCAGAGCC ATAGAGAAAC 
     5461 TCATCAAAAA CAGATCAGTA GTTTGAGAGA TGAAGTAGAA GCAAAAGCAA AACTTATTAC 
     5521 TGATCTTCAA GACCAAAACC AGAAAATGAT GTTAGAGCAG GAACGTCTAA GAGTAGAACA 
     5581 TGAGAAGTTG AAAGCCACAG ATCAGGAAAA GAGCAGAAAA CTACATGAAC TTACGGTTAT 
     5641 GCAAGATAGA CGAGAACAAG CAAGACAAGA CTTGAAGGGT TTGGAAGAGA CAGTGGCAAA 
     5701 AGAACTTCAG ACTTTACACA ACCTGCGCAA ACTCTTTGTT CAGGACCTGG CTACAAGAGT 
     5761 TAAAAAGAGT GCTGAGATTG ATTCTGATGA CACCGGAGGC AGCGCTGCTC AGAAGCAAAA 
     5821 AATCTCCTTT CTTGAAAATA ATCTTGAACA GCTCACTAAA GTGCACAAAC AGTTGGTACG 
     5881 TGATAATGCA GATCTCCGCT GTGAACTTCC TAAGTTGGAA AAGCGACTTC GAGCTACAGC 
     5941 TGAGAGAGTG AAAGCTTTGG AATCAGCACT GAAAGAAGCT AAAGAAAATG CATCTCGTGA 
     6001 TCGCAAACGC TATCAGCAAG AAGTAGATCG CATAAAGGAA GCAGTCAGGT CAAAGAATAT 
     6061 GGCCAGAAGA GGGCATTCTG CACAGATTGC TAAACCTATT CGTCCCGGGC AACATCCAGC 
     6121 AGCTTCTCCA ACTCACCCAA GTGCAATTCG TGGAGGAGGT GCATTTGTTC AGAACAGCCA 
     6181 GCCAGTGGCA GTGCGAGGTG GAGGAGGCAA ACAAGTGACG CGTACGCGGC CGCTCGAGCA 
     6241 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     6301 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6361 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6421 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6481 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6541 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6601 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6661 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6721 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6781 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6841 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6901 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6961 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     7021 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     7081 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7141 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7201 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7261 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7321 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7381 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7441 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7501 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7561 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7621 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7681 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7741 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7801 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7861 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7921 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7981 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     8041 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     8101 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8161 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8221 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8281 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8341 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8401 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8461 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8521 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8581 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8641 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8701 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8761 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8821 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8881 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8941 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     9001 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     9061 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9121 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9181 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     9241 TTTCCCCGAA AAGTGCCACC TGAC
//