LOCUS       RC216777                8142 bp    DNA     circular  SYN 
DEFINITION  EPB41L3 (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1-like 3 (EPB41L3)
ACCESSION   NM_012307
VERSION     NM_012307.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4299..4390 
                     /label="Myc-DDK"  
     misc_feature    4420..5005 
                     /label="PolyA signal"  
     misc_feature    4477..4496 
                     /label="Restriction Enzyme"  
     misc_feature    5281..5953 
                     /label="ColE1"  
     misc_feature    6383..7177 
                     /label="Kan/Neo"  
     misc_feature    7212..7514 
                     /label="SV40 ori"  
     misc_feature    7600..8077 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4281..4287 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4281 
                     /label="EPB41L3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACCGAA TCTGGATCAG ACTCGGAATC CAAGCCGGAC CAGGAGGCCG 
     1081 AGCCCCAGGA GGCGGCGGGG GCGCAGGGGC GCGCGGGGGC GCCCGTGCCG GAGCCGCCCA 
     1141 AGGAGGAGCA GCAGCAGGCC CTGGAGCAGT TCGCCGCCGC TGCAGCGCAC AGCACCCCGG 
     1201 TGCGGAGGGA GGTCACTGAC AAGGAACAGG AGTTTGCTGC CAGGGCTGCA AAACAGCTCG 
     1261 AATATCAGCA ATTAGAAGAC GATAAACTTT CTCAGAAATC ATCTAGCAGT AAACTCTCTC 
     1321 GGTCTCCATT AAAGATTGTC AAAAAGCCTA AAAGCATGCA GTGCAAAGTG ATACTTCTCG 
     1381 ATGGATCAGA ATATACCTGT GATGTAGAGA AACGCTCCAG AGGACAAGTG CTGTTTGATA 
     1441 AAGTGTGTGA ACACTTGAAC TTGCTAGAGA AAGACTACTT TGGGCTTACG TATCGAGATG 
     1501 CTGAAAACCA GAAGAATTGG TTGGACCCTG CTAAGGAAAT AAAAAAACAG GTTCGAAGTG 
     1561 GTGCTTGGCA CTTTTCATTT AATGTGAAAT TTTATCCACC AGACCCTGCC CAACTATCTG 
     1621 AAGATATCAC CAGGTACTAC CTCTGCTTGC AGTTGCGAGA TGACATCGTG TCCGGAAGGC 
     1681 TGCCCTGCTC CTTTGTTACC CTGGCCTTGC TGGGCTCCTA CACTGTCCAG TCAGAGCTCG 
     1741 GAGACTATGA CCCAGATGAA TGTGGGAGCG ATTACATTAG TGAGTTCCGC TTTGCACCAA 
     1801 ACCACACTAA AGAACTGGAA GACAAAGTGA TCGAGCTGCA CAAGAGCCAC AGAGGAATGA 
     1861 CGCCAGCAGA AGCAGAGATG CATTTCTTGG AAAATGCCAA AAAATTATCA ATGTATGGGG 
     1921 TAGATTTACA TCATGCTAAG GACTCAGAAG GGGTAGAAAT TATGTTAGGA GTTTGTGCAA 
     1981 GTGGTCTGTT GATATATCGC GACCGGCTGC GAATAAACAG ATTTGCCTGG CCCAAGGTTC 
     2041 TAAAGATTTC ATACAAACGG AACAACTTTT ACATTAAGAT CCGGCCGGGA GAGTTTGAAC 
     2101 AATTTGAAAG CACCATTGGG TTTAAGCTGC CAAACCATCG AGCTGCCAAG CGTTTATGGA 
     2161 AAGTATGTGT TGAGCATCAT ACATTTTTCA GACTACTGTT ACCAGAAGCA CCTCCCAAGA 
     2221 AATTCCTAAC CTTGGGTTCC AAGTTTCGTT ATAGTGGCAG GACACAAGCG CAAACGAGAA 
     2281 GAGCCAGTGC GTTGATAGAT CGCCCAGCAC CTTACTTTGA ACGCTCATCC AGCAAACGTT 
     2341 ATACCATGTC TCGCAGCTTG GATGGAGAGG TTGGTACTGG CCAGTACGCC ACAACAAAAG 
     2401 GCATCTCTCA GACCAACTTG ATCACCACTG TGACTCCGGA GAAGAAGGCT GAGGAGGAGC 
     2461 GGGACGAGGA AGAGGACAAA CGGAGGAAGG GGGAAGAAGT CACGCCCATC TCGGCCATCC 
     2521 GGCACGAGGG AAAGTCACCT GGGCTTGGCA CTGACTCATG TCCCTTGTCA CCCCCATCCA 
     2581 CCCATTGTGC CCCCACATCT CCCACAGAGC TCCGTAGGAG GTGTAAGGAG AATGACTGCA 
     2641 AACTGCCAGG TTATGAGCCG TCCAGAGCTG AGCACCTGCC TGGAGAGCCC GCCTTGGACT 
     2701 CTGATGGCCC AGGGAGGCCT TACCTAGGGG ATCAAGATGT GGCTTTTAGC TACAGACAGC 
     2761 AAACTGGCAA GGGGACCACC CTGTTCTCCT TCTCCTTGCA GCTCCCTGAG TCATTCCCCT 
     2821 CCCTCCTAGA TGATGATGGA TACCTCTCTT TCCCCAACCT TTCTGAAACC AACCTCCTGC 
     2881 CCCAGAGCTT GCAGCATTAC CTCCCGATCC GCTCACCGTC CCTTGTGCCC TGTTTCCTCT 
     2941 TCATCTTTTT CTTTCTGCTG TCTGCCTCCT TCTCAGTGCC ATACGCTCTC ACTCTCTCCT 
     3001 TCCCTCTGGC TCTGTGCCTC TGCTACCTGG AGCCCAAGGC GGCCTCCTTG AGCGCCTCCC 
     3061 TAGACAATGA CCCGAGTGAC AGTTCAGAGG AAGAGACTGA CAGTGAGCGC ACGGACACCG 
     3121 CAGCCGACGG GGAGACCACC GCCACTGAGT CGGACCAGGA GGAAGATGCA GAGCTCAAGG 
     3181 CACAGGAGCT AGAAAAAACT CAAGATGACC TGATGAAACA TCAAACCAAC ATTAGCGAGC 
     3241 TGAAAAGAAC CTTCTTAGAA ACCTCAACAG ACACTGCCGT AACGAATGAA TGGGAGAAGA 
     3301 GGCTTTCCAC CTCCCCCGTG CGACTGGCCG CCAGGCAGGA GGATGCCCCC ATGATCGAAC 
     3361 CACTTGTCCC TGAAGAGACT AAGCAGTCTT CTGGGGAAAA GCTCATGGAT GGCTCTGAAA 
     3421 TCTTCAGTTT ATTAGAGTCT GCGCGAAAAC CAACAGAATT CATAGGAGGG GTTACTTCTA 
     3481 CTTCTCAAAG CTGGGTTCAG AAAATGGAAA CCAAGACGGA GTCCAGTGGA ATAGAGACGG 
     3541 AACCCACCGT GCACCACCTG CCGCTTAGCA CTGAGAAGGT GGTGCAGGAG ACCGTGTTGG 
     3601 TGGAGGAGCG GCGTGTGGTG CACGCGAGTG GGGATGCTTC TTACTCGGCG GGAGACAGCG 
     3661 GGGATGCTGC AGCACAGCCC GCATTCACAG GCATTAAAGG GAAAGAGGGC TCTGCCTTGA 
     3721 CGGAGGGGGC TAAAGAGGAA GGAGGGGAGG AGGTCGCTAA AGCTGTCCTG GAACAGGAAG 
     3781 AGACAGCCGC TGCTTCCCGT GAGCGACAAG AGGAGCAGAG TGCAGCCATC CACATTTCAG 
     3841 AAACTTTGGA ACAAAAACCT CATTTTGAGT CCTCAACGGT GAAGACGGAA ACCATCAGTT 
     3901 TTGGCAGTGT TTCACCGGGA GGAGTAAAGC TAGAAATTTC CACGAAGGAA GTGCCAGTAG 
     3961 TTCACACCGA AACCAAAACC ATCACATATG AATCATCACA GGTCGATCCA GGCACAGATC 
     4021 TGGAGCCAGG CGTGCTGATG AGTGCACAGA CGATCACATC TGAAACCACC AGTACCACCA 
     4081 CCACTACGCA CATCACCAAA ACTGTGAAAG GGGGCATTTC AGAGACAAGA ATTGAGAAGC 
     4141 GAATAGTCAT CACGGGGGAT GCAGACATTG ACCATGACCA GGCGCTGGCT CAGGCAATTA 
     4201 AAGAGGCCAA AGAGCAGCAC CCTGACATGT CAGTGACCAA AGTAGTGGTC CATAAAGAGA 
     4261 CAGAGATCAC ACCAGAAGAT GGAGAGGATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4321 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4381 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4441 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4501 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4561 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4621 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4681 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4741 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4801 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4861 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4921 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4981 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5041 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5101 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5161 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5221 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5281 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5341 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5401 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5461 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5521 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5581 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5641 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5701 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5761 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5821 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5881 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5941 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6001 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6061 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6121 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6181 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6241 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6301 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6361 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6421 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6481 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6541 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6601 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6661 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6721 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6781 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6841 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6901 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6961 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7021 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7081 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7141 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7201 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7261 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7321 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7381 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7561 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7621 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7681 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7741 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7801 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7861 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7921 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7981 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8041 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8101 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//