LOCUS       RC216720                7956 bp    DNA     circular  SYN 
DEFINITION  MYO1H (Myc-DDK-tagged)-Human myosin IH (MYO1H)
ACCESSION   NM_001101421
VERSION     NM_001101421.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4111..4202 
                     /label="Myc-DDK"  
     misc_feature    4232..4817 
                     /label="PolyA signal"  
     misc_feature    4289..4308 
                     /label="Restriction Enzyme"  
     misc_feature    5093..5765 
                     /label="ColE1"  
     misc_feature    6195..6989 
                     /label="Kan/Neo"  
     misc_feature    7024..7326 
                     /label="SV40 ori"  
     misc_feature    7412..7889 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4086..4093 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4086 
                     /label="MYO1H"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGGGGCG CTGACTGCCC GGGACAAGGT CGGGGTTCAG GATTTTGTGC 
     1081 TATTGGACGC GTACACCAGC GAATCTGCCT TTGTCGACAA CCTCCGCAAG CGTTTCAGCG 
     1141 AGAACCTCAT ATACACATAT ATTGGCACCC TCCTTGTGTC TGTGAATCCA TACCAGGAGC 
     1201 TCGGAATCTA CACTGTGAGC CAGATGGAAC TTTATCAAGG GGTCAATTTC TTTGAACTGC 
     1261 CACCACATGT CTACGCTATA GCCGACAACG CTTACCGAAT GATGTGTGCT GAACTAAATA 
     1321 ACCATTTCAT CCTCATTTCT GGAGAGAGTG GGGCAGGGAA AACAGAGGCC TCCAAGAAAA 
     1381 TTCTCGAGTA TTTTGCAGTG ACCTGCCCAA TGACCCAGTC ACTACAAATA GCCCGTGACA 
     1441 GACTGCTGTT CTCCAACCCA GTGCTGGAGG CTTTTGGAAA TGCCAGAACG CTCCGGAATG 
     1501 ACAACTCCAG CAGATTTGGG AAATACATGG ATATACAATT TGATTTTCAG GGCATTCCCG 
     1561 TAGGTGGGCA TATCATCAGT TACTTGATAG AGAAGTCCCG AGTTGTCTAC CAAAACGAAG 
     1621 GCGAGCGGAA TTTCCACATC TTCTACCAGC TGCTGGCAGG TGGCGAAGAG GAGCGCCTGT 
     1681 CTTACCTGGG ACTCGAGCGA GACCCCCAGC TGTATAAATA CCTCTCACAG GGTCATTGTG 
     1741 CCAAAGAGTC ATCCATTAGT GACAAGAATG ACTGGAAAAC TGTTTCCAAC GCCTTTTCTG 
     1801 TCATTGATTT TACTGAAGCT GACCTCGAGA ATCTCTTTGG AATTATTGCC AGTGTCTTAC 
     1861 ACCTGGGGAA CATTGGTTTT GAAGAAGACG ACCAAGGCTG TGCCACTATC CCAGACACCC 
     1921 ATGAGATCAA GTGGATAGCC AAGCTCCTGG GGGTCCACCC ATCAGTCCTT CTGGAAGCTC 
     1981 TCACCCACAG AAAAATTGAA GCCAAAACTG AGGAGGTGAT CTGCCCGTTG ACACTAGAAC 
     2041 TCTCTGTCTA CGCTAGAGAT GCAATGGCAA AGGCTGTTTA TGGACGAACG TTTACTTGGC 
     2101 TGGTCAACAA AATCAATTCC TCCTTAGTTA ACAAGGATTT CACCAGGAAA ACTGTAATTG 
     2161 GATTACTGGA CATCTATGGG TTTGAAGTCT TTGACAAGAA TGGTTTTGAA CAGTTCTGTA 
     2221 TAAATTACTG CAATGAGAAA CTCCAGCAAC TGTTAATTGA GAGGACTCTA AAAGCAGAAC 
     2281 AGGCAGAATA TGAAATGGAA GGCATAGAGT GGGAGCCAAT TAAATATTTC AACAACAAGA 
     2341 TCATCTGTGA TTTGGTAGAA GAGAGACATA AAGGAATCAT ATCTATTCTG GATGAAGAAT 
     2401 GCATTCGGCC TGGTCCTGCT ACAGACTTGA GTTTCCTGGA GAAATTGGAA GAGAAAGTGG 
     2461 GCAAACATGC ACACTTCGAA ACCCGTAAGC TGGCTGGTCC AAAGGGCCGA AAGAGGATTG 
     2521 GCTGGATGGA GTTCCGACTC CTCCACTATG CAGGAGAGGT CACATACTGC ACCAAGGGAT 
     2581 TCTTGGAAAA AAACAATGAT CTTCTTTACA GACATCTGAA AGAAGTGCTG TGCAAGTCCA 
     2641 AGAACATTAT CCTGAGGGAA TGCTTCCTGC TGGCCGAGTT AGAAAACCGG AGGAGGCCCC 
     2701 CAACAGTGGG GACTCAGTTT AAAAACAGTC TGAGCAGCCT TCTAGAAACC CTCATCTCTA 
     2761 AGGAGCCCTC CTACATCCGT TGCATCAAGC CCAACGACAG GAAAGAACCC AGCAAATTTG 
     2821 ATGACTTCCT CATAAGGCAT CAGATCAAAT ACCTGGGGCT GATGGAGCAC CTGCGGGTGA 
     2881 GACGGGCTGG TTTTGCATAC CGAAGGAAAT ACGAGCATTT CTTGCAAAGG TACAAGTCCT 
     2941 TATGCCCAGA CACCTGGCCG CACTGGCACG GGCCTCCAGC AGAGGGCGTG GAACGGCTGA 
     3001 TCAAGTACAT CGGCTACAAA CCCGAGGAAT ACAAGTTAGG CAAAACCAAA ATATTCATTC 
     3061 GTTTCCCCAG AACTCTGTTT GCTACCGAAG ATGCCTTTGA ATTTAGTAAA CATCAACTAG 
     3121 TTGCAAGAAT CCAAGCCACT TACAAACGCT GCCTAGGAAG GAGAGAATAC GTGAAAAAAA 
     3181 GACAAGCAGC CATCAAACTG GAAGCCCACT GGCGTGGGGC CCTGGCTCGG AAGGCAATCC 
     3241 AAAGGAGAAA GTGGGCCGTG CGGATTATCA GAAAGTTCAT TAAAGGATTC ATCAGTCGCA 
     3301 ACAAACCCCT CTGTCCTGAC AACGAGGAAT TTATCGTGTT TGTGCGGAAG AATTACATCT 
     3361 TGAATCTCCG GTATCACCTT CCAAAGACTG TCCTGGACAA GAGCTGGCTG AGGCCTCCTG 
     3421 GCATCTTGGA AAATGCATCA GATCTGCTCA GGAAAATGTG CGTGAGGAAC CTGGTGCAGA 
     3481 AGTACTGCCG CGGGATCACA GCTGAGCGGA AAGCAATGAT GCAGCAAAAG GTAGTTACAA 
     3541 GTGAAATCTT CAGGGGAAGG AAAGACGGCT ACACAGAAAG TTTAAATCAA CCCTTTGTCA 
     3601 ACAGTCGGAT AGATGAAGGA GACATTAATC CGAAAGTGCT TCAGCTAATT AGCCATGAGA 
     3661 AAATCCAGTA TGGTGTCCCG GTCATTAAAT ATGACAGAAA AGGCTTCAAA GCACGGCAGC 
     3721 GGCAACTCAT TCTTACTCAG AAAGCAGCTT ACGTGGTGGA ACTTGCCAAA ATCAAGCAGA 
     3781 AAATAGAGTA CTCAGCTCTC AAAGGTGTCT CCACTAGCAA TCTGAGTGAT GGAATCTTAG 
     3841 TCATTCATGT TTCACCAGAG GACAGCAAGC AAAAGGGGGA TGCCGTTTTG CAGTGTGGAC 
     3901 ACGTGTTTGA AGCAGTTACT AAACTCGTCA TGCTGGTTAA GAAGGAGAAC ATTGTCAATG 
     3961 TTGTTCAAGG AAGTTTACAG TTTTTTATTA GTCCGGGAAA AGAAGGCACA ATAGTTTTCG 
     4021 ACACTGGACT GGAAGAACAA GTCTATAAAA ATAAAAATGG ACAATTAACA GTGGTGTCAG 
     4081 TCCGGAGGAA GTCCAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//