LOCUS       RC216716                8628 bp    DNA     circular  SYN 
DEFINITION  INF2 (Myc-DDK-tagged)-Human inverted formin, FH2 and WH2 domain containing (INF2), transcript variant 1
ACCESSION   NM_022489
VERSION     NM_022489.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="INF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGAAG GAGGGCGCAC AGCGCAAGTG GGCAGCGCTG AAGGAGAAGC 
     1081 TGGGGCCACA GGATTCGGAC CCCACGGAGG CCAACCTGGA GAGCGCGGAC CCTGAGCTGT 
     1141 GCATCCGGCT GCTCCAGATG CCCTCTGTGG TCAACTACTC CGGCCTGCGC AAGCGCCTGG 
     1201 AGGGCAGCGA CGGCGGCTGG ATGGTGCAGT TCCTGGAGCA GAGCGGCCTG GACCTGCTGC 
     1261 TGGAGGCGCT GGCGCGGCTG TCGGGCCGCG GCGTTGCACG TATCTCCGAC GCCCTGCTGC 
     1321 AGCTCACCTG CGTCAGCTGC GTGCGCGCCG TCATGAACTC GCGGCAGGGC ATCGAGTACA 
     1381 TCCTCAGCAA CCAGGGCTAC GTGCGCCAGC TCTCCCAGGC CCTGGACACA TCCAACGTGA 
     1441 TGGTGAAGAA GCAGGTGTTT GAGCTACTGG CTGCCCTGTG CATCTACTCT CCCGAGGGCC 
     1501 ACGTGCTGAC CCTGGACGCC CTGGACCACT ACAAGACGGT GTGCAGCCAG CAGTACCGCT 
     1561 TCAGCATTGT CATGAACGAG CTCTCCGGCA GCGACAACGT GCCCTACGTG GTCACCCTGC 
     1621 TTAGCGTGAT CAACGCCGTC ATCTTGGGCC CCGAGGACCT GCGCGCGCGC ACCCAGCTGC 
     1681 GGAACGAGTT TATCGGGCTG CAGCTGCTGG ACGTCCTGGC TCGCCTGCGA GACCTGGAGG 
     1741 ATGCCGACCT GCTGATCCAG CTGGAGGCTT TCGAGGAGGC TAAGGCCGAG GACGAGGAGG 
     1801 AGCTGCTGCG AGTCTCTGGC GGGGTCGACA TGAGCAGCCA CCAGGAGGTC TTTGCCTCCC 
     1861 TGTTCCACAA GGTGAGCTGC TCCCCGGTGT CTGCCCAGCT CCTGTCGGTG CTGCAGGGCC 
     1921 TCCTGCACCT GGAGCCCACC CTCCGCTCCA GCCAGCTGCT CTGGGAGGCC CTGGAGAGCC 
     1981 TCGTGAACCG GGCCGTGCTC CTGGCCAGCG ATGCCCAGGA ATGCACCCTG GAGGAAGTGG 
     2041 TTGAGCGGCT CCTGTCTGTC AAGGGGCGAC CCAGACCGAG CCCCCTGGTC AAGGCCCATA 
     2101 AAAGCGTCCA GGCCAACCTA GACCAGAGCC AGAGGGGCAG CTCCCCGCAA AACACTACAA 
     2161 CCCCCAAGCC CAGCGTGGAG GGCCAGCAGC CAGCAGCAGC TGCTGCCTGC GAGCCCGTGG 
     2221 ACCACGCCCA GAGTGAGAGC ATCCTGAAAG TTTCGCAGCC CAGAGCCCTG GAGCAGCAGG 
     2281 CGTCCACCCC ATCCCCACCC CCACCCCCAC CCCTGCTCCC TGGTTCCAGT GCCGAGCCCC 
     2341 CTCCCCCTCC CCCACCACCC CCCCTGCCCA GTGTGGGGGC TAAGGCCCTC CCAACAGCAC 
     2401 CCCCGCCCCC ACCCCTGCCA GGCCTGGGGG CCATGGCCCC CCCAGCACCT CCTCTACCAC 
     2461 CACCCCTGCC AGGCTCCTGT GAGTTCCTGT CCCCACCACC TCCACCACTC CCGGGCTTGG 
     2521 GATGCCCGCC CCCACCCCCA CCCCTGCTGC CTGGTATGGG CTGGGGCCCT CCTCCACCCC 
     2581 CACCTCCACT ACTGCCCTGC ACCTGCAGCC CCCCCGTGGC GGGAGGCATG GAGGAGGTCA 
     2641 TCGTGGCCCA GGTGGACCAT GGCTTGGGCT CAGCATGGGT CCCCAGCCAT CGGCGGGTGA 
     2701 ACCCACCCAC ACTGCGCATG AAGAAGCTGA ACTGGCAGAA GCTGCCATCC AACGTGGCAC 
     2761 GTGAGCACAA CTCTATGTGG GCGTCCCTGA GCAGCCCCGA CGCCGAGGCT GTGGAGCCCG 
     2821 ACTTCTCCAG CATCGAGCGA CTATTCTCCT TCCCTGCAGC CAAGCCCAAG GAGCCCACCA 
     2881 TGGTGGCCCC CCGGGCCAGG AAGGAGCCCA AGGAGATCAC TTTCCTCGAT GCCAAGAAGA 
     2941 GCCTGAACCT CAACATCTTC CTGAAGCAAT TTAAGTGCTC CAACGAGGAG GTCGCTGCTA 
     3001 TGATCCGGGC TGGAGATACC ACCAAGTTTG ATGTGGAGGT TCTCAAACAA CTCCTTAAGC 
     3061 TCCTTCCCGA GAAGCACGAG ATTGAAAACC TGCGGGCATT CACAGAGGAG CGAGCCAAGC 
     3121 TGGCCAGCGC CGACCACTTC TACCTCCTCC TGCTGGCCAT TCCCTGCTAC CAGCTGCGAA 
     3181 TCGAGTGCAT GCTGCTGTGT GAGGGCGCGG CCGCCGTGCT GGACATGGTG CGGCCCAAGG 
     3241 CCCAGCTGGT GCTGGCTGCC TGCGAAAGCC TGCTCACCAG CCGCCAGCTG CCCATCTTCT 
     3301 GCCAGCTGAT CCTGAGAATT GGGAACTTCC TCAACTACGG CAGCCACACC GGTGACGCCG 
     3361 ACGGCTTCAA GATCAGCACA TTGCTGAAGC TCACGGAGAC CAAGTCCCAG CAGAACCGCG 
     3421 TGACGCTGCT GCACCACGTG CTGGAGGAAG CGGAAAAGAG CCACCCCGAC CTCCTGCAGC 
     3481 TGCCCCGGGA CCTGGAACAG CCCTCGCAAG CAGCAGGGAT CAACCTGGAG ATCATCCGCT 
     3541 CAGAGGCCAG CTCCAACCTG AAGAAGCTTC TGGAGACCGA GCGGAAGGTG TCTGCCTCCG 
     3601 TGGCCGAGGT CCAGGAGCAG TACACCGAGC GCCTCCAGGC CAGCATCTCG GCCTTCCGGG 
     3661 CACTGGATGA GCTGTTTGAG GCCATCGAGC AGAAGCAACG GGAGCTGGCC GACTACCTGT 
     3721 GTGAGGACGC CCAGCAGCTG TCCCTGGAGG ACACGTTCAG CACCATGAAG GCTTTCCGGG 
     3781 ACCTTTTCCT CCGCGCCCTG AAGGAGAACA AGGACCGGAA GGAGCAGGCG GCGAAGGCAG 
     3841 AGAGGAGGAA GCAGCAGCTG GCGGAGGAGG AGGCGCGGCG GCCTCGGGGA GAGGACGGGA 
     3901 AGCCTGTCAG GAAGGGGCCC GGGAAGCAGG AGGAGGTGTG TGTCATCGAT GCCCTGCTGG 
     3961 CTGACATCAG GAAGGGCTTC CAGCTGCGGA AGACAGCCCG GGGCCGCGGG GACACCGACG 
     4021 GGGGCAGCAA GGCAGCCTCC ATGGATCCCC CAAGAGCCAC AGAGCCTGTG GCCACCAGTA 
     4081 ACCCTGCAGG AGACCCCGTG GGCAGCACGC GCTGTCCCGC CTCTGAGCCC GGCCTTGATG 
     4141 CTACAACAGC CAGCGAGTCC CGGGGCTGGG ACCTTGTAGA CGCCGTGACC CCCGGCCCTC 
     4201 AGCCCACCCT GGAGCAGTTG GAGGAGGGTG GTCCCCGGCC CCTGGAGAGG CGTTCTTCCT 
     4261 GGTATGTGGA TGCCAGCGAT GTCCTAACCA CTGAGGATCC CCAGTGCCCC CAGCCCTTGG 
     4321 AGGGGGCCTG GCCGGTGACT CTGGGAGATG CTCAGGCCCT GAAGCCCCTC AAGTTCTCCA 
     4381 GCAACCAGCC CCCTGCAGCC GGAAGTTCAA GGCAAGATGC CAAGGATCCC ACGTCCTTGC 
     4441 TGGGCGTCCT CCAGGCCGAG GCCGACAGCA CAAGTGAGGG GCTGGAGGAC GCTGTCCACA 
     4501 GCCGTGGTGC CAGACCCCCT GCAGCAGGCC CAGGTGGGGA TGAGGACGAG GACGAGGAGG 
     4561 ACACGGCCCC AGAGTCCGCA CTGGACACAT CCCTGGACAA GTCCTTCTCC GAGGATGCGG 
     4621 TGACCGACTC CTCGGGGTCG GGCACACTCC CCAGGGCCCG GGGCCGGGCC TCAAAGGGGA 
     4681 CCGGGAAGCG AAGGAAGAAG CGTCCCTCCA GGAGCCAGGA AGAGGTTCCC CCTGATTCTG 
     4741 ATGATAATAA AACAAAGAAA CTGTGTGTGA TCCAGACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//