LOCUS       RC216634                7644 bp    DNA     circular  SYN 
DEFINITION  HK1 (Myc-DDK-tagged)-Human hexokinase 1 (HK1), nuclear gene encoding mitochondrial protein, transcript variant 3
ACCESSION   NM_033497
VERSION     NM_033497.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3801..3892 
                     /label="Myc-DDK"  
     misc_feature    3922..4507 
                     /label="PolyA signal"  
     misc_feature    3979..3998 
                     /label="Restriction Enzyme"  
     misc_feature    4783..5455 
                     /label="ColE1"  
     misc_feature    5885..6679 
                     /label="Kan/Neo"  
     misc_feature    6714..7016 
                     /label="SV40 ori"  
     misc_feature    7102..7579 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3783..3789 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3783 
                     /label="HK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCAGATC TGCCAGCGAG AATCGGCTAC AGCAGCTGAA AAACCAAAAC 
     1081 TTCATCTACT TGCTGAAAGT GAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCCGATG 
     1141 AAACTCTCAT AGATATCATG ACTCGCTTCA GGAAGGAGAT GAAGAATGGC CTCTCCCGGG 
     1201 ATTTTAATCC AACAGCCACA GTCAAGATGT TGCCAACATT CGTAAGGTCC ATTCCTGATG 
     1261 GCTCTGAAAA GGGAGATTTC ATTGCCCTGG ATCTTGGTGG GTCTTCCTTT CGAATTCTGC 
     1321 GGGTGCAAGT GAATCATGAG AAAAACCAGA ATGTTCACAT GGAGTCCGAG GTTTATGACA 
     1381 CCCCAGAGAA CATCGTGCAC GGCAGTGGAA GCCAGCTTTT TGATCATGTT GCTGAGTGCC 
     1441 TGGGAGATTT CATGGAGAAA AGGAAGATCA AGGACAAGAA GTTACCTGTG GGATTCACGT 
     1501 TTTCTTTTCC TTGCCAACAA TCCAAAATAG ATGAGGCCAT CCTGATCACC TGGACAAAGC 
     1561 GATTTAAAGC GAGCGGAGTG GAAGGAGCAG ATGTGGTCAA ACTGCTTAAC AAAGCCATCA 
     1621 AAAAGCGAGG GGACTATGAT GCCAACATCG TAGCTGTGGT GAATGACACA GTGGGCACCA 
     1681 TGATGACCTG TGGCTATGAC GACCAGCACT GTGAAGTCGG CCTGATCATC GGCACTGGCA 
     1741 CCAATGCTTG CTACATGGAG GAACTGAGGC ACATTGATCT GGTGGAAGGA GACGAGGGGA 
     1801 GGATGTGTAT CAATACAGAA TGGGGAGCCT TTGGAGACGA TGGATCATTA GAAGACATCC 
     1861 GGACAGAGTT TGACAGGGAG ATAGACCGGG GATCCCTCAA CCCTGGAAAA CAGCTGTTTG 
     1921 AGAAGATGGT CAGTGGCATG TACTTGGGAG AGCTGGTTCG ACTGATCCTA GTCAAGATGG 
     1981 CCAAGGAGGG CCTCTTATTT GAAGGGCGGA TCACCCCGGA GCTGCTCACC CGAGGGAAGT 
     2041 TTAACACCAG TGATGTGTCA GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG 
     2101 AAATCCTGAC CCGCCTGGGA GTGGAGCCGT CCGATGATGA CTGTGTCTCA GTCCAGCACG 
     2161 TTTGCACCAT TGTCTCATTT CGCTCAGCCA ACTTGGTGGC TGCCACACTG GGCGCCATCT 
     2221 TGAACCGCCT GCGTGATAAC AAGGGCACAC CCAGGCTGCG GACCACGGTT GGTGTCGACG 
     2281 GATCTCTTTA CAAGACGCAC CCACAGTATT CCCGGCGTTT CCACAAGACT CTAAGGCGCT 
     2341 TGGTGCCAGA CTCCGATGTG CGCTTCCTCC TCTCGGAGAG TGGCAGCGGC AAGGGGGCTG 
     2401 CCATGGTGAC GGCGGTGGCC TACCGCTTGG CCGAGCAGCA CCGGCAGATA GAGGAGACCC 
     2461 TGGCTCATTT CCACCTCACC AAGGACATGC TGCTGGAGGT GAAGAAGAGG ATGCGGGCCG 
     2521 AGATGGAGCT GGGGCTGAGG AAGCAGACGC ACAACAATGC CGTGGTTAAG ATGCTGCCCT 
     2581 CCTTCGTCCG GAGAACTCCC GACGGGACCG AGAATGGTGA CTTCTTGGCC CTGGATCTTG 
     2641 GAGGAACCAA TTTCCGTGTG CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGAACGGTGG 
     2701 AAATGCACAA CAAGATCTAC GCCATTCCTA TTGAAATCAT GCAGGGCACT GGGGAAGAGC 
     2761 TGTTTGATCA CATTGTCTCC TGCATCTCTG ACTTCTTGGA CTACATGGGG ATCAAAGGCC 
     2821 CCAGGATGCC TCTGGGCTTC ACGTTCTCAT TTCCCTGCCA GCAGACGAGT CTGGACGCGG 
     2881 GAATCTTGAT CACGTGGACA AAGGGTTTTA AGGCAACAGA CTGCGTGGGC CACGATGTAG 
     2941 TCACCTTACT AAGGGATGCG ATAAAAAGGA GAGAGGAATT TGACCTGGAC GTGGTGGCTG 
     3001 TGGTCAACGA CACAGTGGGC ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGTGAGG 
     3061 TTGGACTCAT TGTTGGGACC GGCAGCAATG CCTGCTACAT GGAGGAGATG AAGAACGTGG 
     3121 AGATGGTGGA GGGGGACCAG GGGCAGATGT GCATCAACAT GGAGTGGGGG GCCTTTGGGG 
     3181 ACAACGGGTG TCTGGATGAT ATCAGGACAC ACTACGACAG ACTGGTGGAC GAATATTCCC 
     3241 TAAATGCTGG GAAACAAAGG TATGAGAAGA TGATCAGTGG TATGTACCTG GGTGAAATCG 
     3301 TCCGCAACAT CTTAATCGAC TTCACCAAGA AGGGATTCCT CTTCCGAGGG CAGATCTCTG 
     3361 AGACGCTGAA GACCCGGGGC ATCTTTGAGA CCAAGTTTCT CTCTCAGATC GAGAGTGACC 
     3421 GATTAGCACT GCTCCAGGTC CGGGCTATCC TCCAGCAGCT AGGTCTGAAT AGCACCTGCG 
     3481 ATGACAGTAT CCTCGTCAAG ACAGTGTGCG GGGTGGTGTC CAGGAGGGCC GCACAGCTGT 
     3541 GTGGCGCAGG CATGGCTGCG GTTGTGGATA AGATCCGCGA GAACAGAGGA CTGGACCGTC 
     3601 TGAATGTGAC TGTGGGAGTG GACGGGACAC TCTACAAGCT TCATCCACAC TTCTCCAGAA 
     3661 TCATGCACCA GACGGTGAAG GAACTGTCAC CAAAATGTAA CGTGTCCTTC CTCCTGTCTG 
     3721 AGGATGGCAG CGGCAAGGGG GCCGCCCTCA TCACGGCCGT GGGCGTGCGG TTACGCACAG 
     3781 AGGCAAGCAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//