LOCUS       RC216461                8391 bp    DNA     circular  SYN 
DEFINITION  MTMR3 (Myc-DDK-tagged)-Human myotubularin related protein 3 (MTMR3), transcript variant 1
ACCESSION   NM_153050
VERSION     NM_153050.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4548..4639 
                     /label="Myc-DDK"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    4726..4745 
                     /label="Restriction Enzyme"  
     misc_feature    5530..6202 
                     /label="ColE1"  
     misc_feature    6632..7426 
                     /label="Kan/Neo"  
     misc_feature    7461..7763 
                     /label="SV40 ori"  
     misc_feature    7849..8326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4530..4536 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4530 
                     /label="MTMR3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAAGAG ACTCGGCACA GCCTTGAGTG CATCCAGGCC AATCAGATCT 
     1081 TTCCCAGGAA GCAGCTGATC CGGGAGGATG AGAATCTTCA GGTTCCTTTC CTTGAACTTC 
     1141 ATGGAGAGAG CACAGAGTTT GTGGGCCGTG CCGAGGATGC CATCATTGCC CTTTCCAATT 
     1201 ACAGACTTCA CATCAAGTTC AAGGAGTCTC TTGTTAATGT TCCATTACAG CTTATAGAAA 
     1261 GTGTTGAATG CCGAGATATA TTTCAGCTTC ATTTGACTTG CAAAGACTGC AAAGTTATCA 
     1321 GGTGTCAGTT TTCAACCTTT GAGCAGTGTC AAGAGTGGCT GAAGAGACTG AACAACGCAA 
     1381 TCCGACCACC TGCTAAAATA GAAGATCTCT TCTCATTTGC ATACCATGCT TGGTGCATGG 
     1441 AGGTCTATGC CAGTGAAAAA GAGCAACATG GAGACCTGTG CAGACCAGGG GAGCATGTAA 
     1501 CTTCAAGGTT TAAAAACGAG GTGGAGAGGA TGGGTTTTGA TATGAACAAC GCCTGGAGGA 
     1561 TTTCCAACAT CAATGAGAAG TACAAATTAT GTGGTAGCTA TCCTCAAGAG CTCATAGTGC 
     1621 CTGCCTGGAT CACTGACAAA GAACTGGAAA GTGTATCAAG TTTCAGGTCC TGGAAGCGCA 
     1681 TCCCTGCCGT CATCTACAGG CACCAGAGCA ATGGAGCTGT CATTGCCCGC TGTGGACAGC 
     1741 CAGAGGTTAG CTGGTGGGGC TGGCGAAATG CAGATGATGA GCATCTGGTA CAGTCAGTAG 
     1801 CCAAAGCTTG TGCCTCTGAC TCCCGATCGA GTGGCAGCAA GCTGTCAACT AGGAACACTT 
     1861 CTCGAGACTT TCCCAATGGG GGAGACCTTT CTGACGTGGA GTTCGATTCT TCTCTGTCAA 
     1921 ATGCTTCAGG AGCAGAGAGT TTAGCCATCC AACCGCAGAA GCTTTTGATC TTGGATGCAC 
     1981 GCTCCTATGC AGCTGCTGTG GCAAACCGAG CCAAAGGAGG AGGCTGCGAA TGCCCAGAGT 
     2041 ATTACCCAAA CTGTGAAGTT GTGTTTATGG GGATGGCAAA CATTCATTCT ATTCGGAGGA 
     2101 GTTTTCAGTC TCTGCGGTTG CTGTGCACTC AGATGCCAGA TCCGGGAAAT TGGCTATCAG 
     2161 CTCTTGAAAG CACAAAATGG CTCCATCACT TGTCTGTGCT TCTGAAATCA GCGCTTCTGG 
     2221 TAGTGCATGC TGTGGATCAG GATCAGCGGC CGGTGCTAGT ACACTGCTCA GATGGCTGGG 
     2281 ACCGCACCCC CCAGATTGTG GCATTGGCTA AGCTCTTGCT GGACCCTTAT TACCGAACCA 
     2341 TAGAGGGTTT CCAGGTCCTC GTGGAAATGG AGTGGCTGGA TTTTGGCCAT AAATTTGCTG 
     2401 ACCGGTGTGG TCATGGGGAG AACTCGGATG ATCTGAATGA ACGTTGCCCA GTGTTTCTGC 
     2461 AGTGGCTTGA CTGTGTTCAT CAGCTTCAGA GGCAATTTCC TTGCTCTTTT GAGTTCAATG 
     2521 AAGCATTCCT TGTGAAACTG GTGCAGCATA CCTATTCCTG CCTGTTTGGA ACATTCCTGT 
     2581 GCAACAACGC CAAGGAGAGA GGGGAAAAGC ATACTCAGGA ACGGACATGT TCCGTGTGGT 
     2641 CACTTCTTCG GGCAGGCAAC AAGGCTTTCA AAAACCTACT GTATTCCTCT CAGTCAGAAG 
     2701 CCGTGCTGTA CCCTGTGTGC CATGTGCGTA ACCTGATGCT GTGGAGTGCA GTGTACCTGC 
     2761 CCTGCCCATC CCCAACCACC CCTGTGGACG ACAGCTGTGC ACCATACCCA GCCCCAGGCA 
     2821 CCAGCCCTGA TGATCCCCCC CTGAGCCGGC TACCAAAGAC TAGATCATAC GACAATCTGA 
     2881 CCACAGCCTG TGACAACACA GTGCCTCTGG CCAGCCGGCG CTGCAGCGAC CCCAGCCTGA 
     2941 ACGAGAAGTG GCAGGAGCAC CGGCGCTCAC TAGAGCTGAG CAGCCTGGCT GGCCCTGGAG 
     3001 AGGATCCCCT TTCTGCCGAC AGCCTAGGGA AGCCCACCAG AGTGCCGGGG GGTGCCGAGC 
     3061 TTTCTGTTGC AGCCGGAGTA GCTGAGGGGC AGATGGAGAA CATCTTGCAG GAGGCCACCA 
     3121 AAGAGGAGAG TGGAGTAGAG GAACCTGCCC ACAGGGCAGG CATTGAGATA CAGGAGGGTA 
     3181 AAGAGGACCC TCTCTTAGAA AAGGAGAGCA GGAGGAAGAC ACCTGAGGCC TCAGCCATTG 
     3241 GACTTCACCA AGACCCAGAA CTGGGTGATG CTGCTCTGAG GAGCCATCTG GATATGAGCT 
     3301 GGCCTCTGTT CTCACAGGGC ATTTCTGAAC AGCAGAGTGG GCTCAGTGTT CTCCTCAGTT 
     3361 CTCTCCAGGT CCCCCCCAGG GGAGAGGATT CCCTGGAGGT CCCTGTGGAG CAGTTTCGAA 
     3421 TAGAAGAGAT TGCAGAGGGT AGGGAGGAAG CAGTTCTTCC AATCCCAGTA GATGCAAAAG 
     3481 TTGGCTATGG TACCTCACAG TCATGTTCTC TGCTACCTTC CCAAGTCCCT TTTGAGACCA 
     3541 GAGGACCAAA CGTGGACAGT TCTACAGACA TGTTAGTGGA AGATAAGGTG AAGTCAGTAA 
     3601 GTGGGCCCCA AGGTCATCAT AGATCTTGCC TTGTAAATAG TGGCAAGGAC AGGCTTCCTC 
     3661 AGACCATGGA ACCCAGCCCT TCAGAGACAA GCCTGGTCGA GAGGCCCCAA GTGGGGTCTG 
     3721 TGGTGCATAG GACTTCCCTT GGCAGCACTC TCAGCCTGAC ACGTTCCCCT TGTGCCTTGC 
     3781 CTTTAGCCGA ATGTAAAGAG GGGCTTGTGT GCAATGGTGC CCCAGAGACT GAAAACAGGG 
     3841 CCTCAGAGCA GCCCCCAGGT CTTAGCACCC TCCAGATGTA CCCCACACCC AATGGGCATT 
     3901 GCGCCAATGG GGAGGCTGGT AGGAGCAAGG ACTCACTGAG CCGTCAGCTG TCTGCTATGA 
     3961 GCTGCAGCTC TGCCCACTTA CACTCAAGGA ACTTGCACCA CAAGTGGCTG CATAGCCACT 
     4021 CAGGAAGGCC ATCTGCAACC AGCAGCCCCG ACCAGCCTTC CCGCAGCCAC CTGGACGATG 
     4081 ATGGCATGTC AGTGTACACA GACACGATCC AACAGCGCCT GCGTCAGATT GAGTCAGGCC 
     4141 ACCAGCAGGA AGTAGAAACT TTGAAGAAAC AAGTCCAGGA GCTGAAGAGT CGCCTGGAGA 
     4201 GCCAGTACCT GACCAGCTCC CTACACTTTA ATGGAGACTT TGGGGATGAG GTGATGACCC 
     4261 GTTGGCTTCC TGACCACCTG GCCGCCCACT GCTATGCGTG CGACAGTGCC TTCTGGCTTG 
     4321 CCAGCAGGAA GCACCACTGC AGGGACACTG ACCGTGTTGA TCAAACGTGG AATTGTGGGA 
     4381 ACGTATTCTG CTCCAGTTGT TGTAACCAGA AGGTTCCAGT TCCCAGCCAG CAGCTCTTTG 
     4441 AACCCAGTCG AGTATGCAAG TCTTGCTATA GCAGCCTACA TCCCACAAGC TCCAGCATTG 
     4501 ACCTTGAACT GGATAAGCCC ATTGCTGCCA CTTCCAACAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//