LOCUS       RC216422                8928 bp    DNA     circular  SYN 
DEFINITION  PITPNM2 (Myc-DDK-tagged)-Human phosphatidylinositol transfer protein, membrane-associated 2 (PITPNM2)
ACCESSION   NM_020845
VERSION     NM_020845.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5085..5176 
                     /label="Myc-DDK"  
     misc_feature    5206..5791 
                     /label="PolyA signal"  
     misc_feature    5263..5282 
                     /label="Restriction Enzyme"  
     misc_feature    6067..6739 
                     /label="ColE1"  
     misc_feature    7169..7963 
                     /label="Kan/Neo"  
     misc_feature    7998..8300 
                     /label="SV40 ori"  
     misc_feature    8386..8863 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5067..5073 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5067 
                     /label="PITPNM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTATAAAG GAATATCGGA TTCCTCTGCC AATGACCGTG GAGGAGTACC 
     1081 GCATCGCCCA GCTGTACATG ATACAGAAGA AGAGCCGTAA CGAGACATAT GGCGAAGGCA 
     1141 GCGGCGTGGA GATCCTGGAG AACCGGCCGT ACACAGATGG CCCAGGCGGC TCTGGGCAGT 
     1201 ACACACACAA GGTGTATCAT GTGGGCATGC ACATTCCCAG CTGGTTCCGC TCCATCCTGC 
     1261 CCAAGGCAGC CCTGCGGGTG GTGGAGGAGT CTTGGAATGC CTACCCCTAC ACCCGAACCA 
     1321 GGTTCACCTG TCCTTTCGTG GAGAAATTCT CCATCGACAT TGAAACCTTT TATAAAACTG 
     1381 ATGCTGGAGA AAACCCCGAC GTGTTCAACC TCTCTCCTGT GGAAAAGAAC CAGCTGACAA 
     1441 TCGACTTCAT CGACATTGTC AAAGACCCTG TGCCCCACAA CGAGTATAAG ACAGAAGAGG 
     1501 ACCCCAAGCT GTTCCAGTCA ACCAAGACCC AGCGGGGGCC CCTGTCCGAG AACTGGATCG 
     1561 AGGAGTACAA GAAGCAGGTC TTCCCCATCA TGTGCGCATA CAAGCTCTGC AAGGTGGAGT 
     1621 TCCGCTACTG GGGCATGCAG TCCAAGATCG AGAGGTTCAT CCACGACACC GGACTACGGA 
     1681 GGGTGATGGT GCGGGCTCAC CGGCAGGCCT GGTGCTGGCA GGACGAGTGG TATGGGCTGA 
     1741 GCATGGAGAA CATCCGGGAG CTGGAGAAGG AGGCACAGCT CATGCTTTCC CGTAAGATGG 
     1801 CCCAGTTCAA TGAGGATGGT GAGGAGGCCA CTGAGCTCGT CAAGCACGAA GCCGTCTCGG 
     1861 ACCAGACCTC TGGGGAGCCC CCGGAGCCCA GCAGCAGCAA TGGGGAGCCC CTAGTGGGGC 
     1921 GCGGCCTCAA GAAACAGTGG TCCACATCCT CCAAGTCGTC TCGGTCGTCC AAGCGGGGAG 
     1981 CGAGTCCTTC CCGCCACAGC ATCTCAGAGT GGAGGATGCA GAGTATTGCC AGGGACTCGG 
     2041 ATGAGAGCTC AGATGATGAG TTCTTCGATG CGCACGAGGA CCTGTCCGAC ACAGAGGAAA 
     2101 TGTTCCCCAA GGACATCACC AAGTGGAGCT CCAATGACCT CATGGACAAG ATCGAGAGCC 
     2161 CAGAGCCGGA AGACACACAA GATGGTCTGT ACCGCCAGGG TGCCCCTGAG TTCAGGGTGG 
     2221 CCTCCAGTGT GGAGCAGCTG AACATCATAG AGGACGAGGT TAGCCAGCCG CTGGCTGCAC 
     2281 CGCCCTCCAA GATCCACGTG CTGCTACTGG TGCTGCACGG AGGCACCATC CTGGACACAG 
     2341 GCGCCGGGGA CCCCAGCTCC AAGAAGGGCG ATGCTAACAC CATCGCCAAC GTGTTCGACA 
     2401 CCGTCATGCG CGTGCACTAC CCCAGCGCCC TGGGCCGCCT TGCCATCCGC CTGGTGCCCT 
     2461 GCCCGCCCGT CTGCTCTGAC GCCTTTGCCC TGGTCTCCAA CCTCAGCCCC TACAGCCATG 
     2521 ACGAAGGCTG TCTGTCCAGC AGTCAGGACC ACATTCCCCT GGCTGCCCTC CCCCTGCTGG 
     2581 CCACCTCCTC CCCCCAGTAC CAGGAGGCAG TTGCCACAGT GATTCAGCGA GCCAACCTTG 
     2641 CCTATGGGGA CTTCATCAAG TCCCAGGAGG GCATGACCTT CAATGGGCAG GTCTGCCTGA 
     2701 TTGGGGACTG CGTCGGGGGC ATCCTGGCAT TTGATGCCCT GTGCTACAGT AACCAGCCGG 
     2761 TGTCTGAGAG TCAGAGCAGC AGCCGCCGGG GCAGCGTGGT CAGCATGCAG GACAATGACC 
     2821 TGCTGTCCCC GGGCATCCTG ATGAATGCAG CACACTGCTG CGGTGGTGGC GGTGGCGGCG 
     2881 GTGGCGGTGG TGGCAGCAGT GGTGGTGGTG GCAGTAGTGG TGGCTCCAGC CTGGAGAGCA 
     2941 GTCGGCACCT GAGCCGAAGC AACGTCGACA TCCCCCGCAG CAACGGCACT GAGGACCCCA 
     3001 AAAGGCAACT GCCCCGCAAG AGGAGCGACT CATCCACCTA CGAGCTGGAT ACCATCCAGC 
     3061 AGCACCAGGC CTTCCTGTCC AGCCTCCATG CCAGCGTGCT GAGGACTGAG CCCTGCTCAC 
     3121 GCCATTCCAG CAGCTCCACC ATGCTGGATG GCACAGGTGC CCTGGGCAGG TTTGACTTTG 
     3181 AGATCACCGA CCTCTTCCTC TTCGGGTGCC CGCTGGGGCT GGTCCTGGCC TTGAGGAAGA 
     3241 CTGTCATCCC AGCCCTGGAT GTTTTCCAGC TGCGGCCGGC CTGCCAGCAA GTCTACAACC 
     3301 TCTTCCACCC CGCGGACCCG TCAGCTTCAC GCCTGGAGCC GCTGCTGGAA CGGCGCTTTC 
     3361 ACGCCCTGCC GCCTTTCAGC GTCCCCCGCT ACCAACGCTA CCCGCTGGGG GATGGCTGCT 
     3421 CCACGCTGCT GGCGGATGTG CTCCAGACCC ACAATGCAGC CTTCCAAGAG CATGGCGCCC 
     3481 CCTCCTCGCC GGGCACTGCC CCTGCCAGTC GTGGCTTCCG CCGAGCCAGT GAGATCAGCA 
     3541 TCGCCAGCCA GGTGTCAGGC ATGGCTGAGA GCTACACGGC ATCCAGCATC GCCCAGAAGG 
     3601 CCCCCGATGC GCTCAGCCAT ACCCCCAGCG TCAGGCGTCT GTCCCTGCTC GCCCTGCCCG 
     3661 CCCCCAGCCC CACCACCCCT GGCCCCCACC CTCCAGCCAG GAAGGCAAGC CCTGGCCTGG 
     3721 AGAGGGCCCC TGGCCTCCCT GAGCTGGACA TTGGAGAAGT CGCTGCAAAG TGGTGGGGCC 
     3781 AGAAGCGGAT CGACTACGCC CTGTACTGCC CTGACGCCCT CACGGCCTTC CCCACGGTGG 
     3841 CTCTGCCTCA CCTCTTCCAC GCCAGCTACT GGGAGTCAAC AGACGTGGTC TCCTTTCTGC 
     3901 TGAGACAGGT CATGAGGCAT GACAACTCCA GCATCTTGGA GCTGGATGGC AAGGAAGTGT 
     3961 CGGTGTTCAC CCCCTCAAAG CCAAGGGAGA AGTGGCAGCG CAAGCGGACC CACGTGAAGC 
     4021 TGCGGAACGT GACGGCCAAC CACCGGATCA ATGATGCCCT TGCCAATGAG GACGGCCCCC 
     4081 AGGTTCTGAC GGGCAGGTTC ATGTATGGGC CCCTGGACAT GGTCACCCTG ACTGGGGAGA 
     4141 AGGTGGATGT GCACATCATG ACCCAGCCGC CCTCAGGCGA GTGGCTCTAC CTGGATACGC 
     4201 TGGTGACCAA CAACAGTGGG CGTGTCTCCT ACACCATCCC TGAGTCGCAC CGCCTGGGCG 
     4261 TGGGTGTCTA CCCTATCAAG ATGGTGGTCA GGGGAGACCA CACGTTTGCC GACAGCTACA 
     4321 TCACCGTGCT GCCCAAGGGC ACAGAGTTCG TGGTCTTCAG CATCGACGGT TCCTTTGCCG 
     4381 CTAGCGTGTC CATCATGGGC AGCGACCCCA AGGTGCGGGC CGGGGCCGTG GACGTGGTGC 
     4441 GGCACTGGCA GGACCTGGGC TACCTCATCA TCTACGTGAC GGGCCGGCCC GACATGCAGA 
     4501 AGCAGCGGGT GGTGGCGTGG CTGGCCCAGC ACAACTTCCC CCATGGCGTG GTGTCCTTCT 
     4561 GTGACGGCCT GGTGCATGAC CCGCTGCGGC ACAAGGCCAA CTTCCTGAAG CTGCTCATCT 
     4621 CCGAGCTGCA CCTGCGCGTG CACGCGGCCT ATGGCTCCAC CAAGGACGTG GCGGTGTACA 
     4681 GCGCCATTAG CCTGTCCCCC ATGCAGATCT ACATCGTGGG CCGGCCCACC AAGAAGCTGC 
     4741 AGCAGCAGTG CCAGTTCATC ACGGATGGCT ACGCGGCCCA CCTGGCGCAG CTGAAGTACA 
     4801 GCCACCGGGC GCGGCCCGCT CGCAACACGG CCACCCGCAT GGCGCTGCGC AAGGGCAGCT 
     4861 TCGGCCTGCC CGGCCAGGGC GACTTTCTGC GCTCCCGGAA CCACCTGCTT CGCACCATCT 
     4921 CGGCCCAGCC CAGCGGGCCC AGCCACCGGC ACGAGCGGAC ACAGAGCCAG GCGGATGGCG 
     4981 AGCAGCGGGG CCAGCGCAGC ATGAGTGTGG CGGCCGGCTG CTGGGGCCGC GCCATGACTG 
     5041 GCCGCCTGGA GCCGGGGGCA GCCGCGGGCC CCAAGACGCG TACGCGGCCG CTCGAGCAGA 
     5101 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5161 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5221 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5281 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5341 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5401 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5461 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5521 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5581 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5641 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5701 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5761 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5821 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5881 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5941 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6001 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6061 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6121 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6181 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6241 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6301 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6361 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6421 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6481 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6541 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6601 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6661 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6721 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6781 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6841 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6901 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6961 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7021 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7081 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7141 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7201 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7261 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7321 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7381 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7441 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7501 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7561 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7621 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7681 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7741 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7801 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7861 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7921 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7981 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8041 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8101 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8161 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8221 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8281 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8341 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8401 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8461 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8521 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8581 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8641 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8701 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8761 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8821 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8881 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//