LOCUS       RC216411                9039 bp    DNA     circular  SYN 
DEFINITION  ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3)
ACCESSION   NM_022370
VERSION     NM_022370.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5196..5287 
                     /label="Myc-DDK"  
     misc_feature    5317..5902 
                     /label="PolyA signal"  
     misc_feature    5374..5393 
                     /label="Restriction Enzyme"  
     misc_feature    6178..6850 
                     /label="ColE1"  
     misc_feature    7280..8074 
                     /label="Kan/Neo"  
     misc_feature    8109..8411 
                     /label="SV40 ori"  
     misc_feature    8497..8974 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5178..5184 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5178 
                     /label="ROBO3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCGCTAC CTGCTGAAAA CGCTGCTGCA GATGAACTTG TTCGCGGACT 
     1081 CTCTGGCCGG GGACATCTCC AACTCCAGCG AGCTGCTCTT GGGCTTCAAC TCCTCGCTGG 
     1141 CGGCGCTCAA CCACACCCTG CTGCCTCCCG GCGATCCCTC TCTCAACGGG TCAAGGGTAG 
     1201 GACCGGAGGA CGCTATGCCC CGCATCGTGG AGCAGCCGCC AGATCTGCTG GTCTCCCGAG 
     1261 GCGAGCCCGC CACGTTGCCC TGCCGCGCTG AAGGCCGACC CCGACCCAAC ATTGAGTGGT 
     1321 ACAAGAACGG GGCGCGTGTG GCCACTGTGC GGGAGGATCC GCGTGCGCAC CGCCTGCTGC 
     1381 TGCCCAGCGG CGCCCTCTTC TTCCCGCGCA TCGTGCACGG GCGCCGCGCG CGGCCGGACG 
     1441 AAGGTGTCTA CACTTGCGTG GCTCGCAACT ACCTGGGGGC AGCAGCGAGC AGAAACGCCT 
     1501 CGCTGGAAGT GGCAGTCCTC CGTGATGATT TCCGGCAGTC TCCTGGAAAC GTGGTGGTGG 
     1561 CAGTGGGGGA GCCAGCAGTA CTGGAATGCG TGCCCCCCCG CGGCCACCCG GAGCCTTCCG 
     1621 TGTCCTGGAG GAAGGACGGT GCAAGACTCA AGGAAGAGGA AGGAAGGATC ACGATCCGTG 
     1681 GAGGGAAGCT GATGATGTCA CATACACTCA AGAGCGATGC AGGCATGTAT GTGTGCGTAG 
     1741 CCTCCAACAT GGCGGGAGAA CGGGAGAGTG CGGCAGCTGA AGTCATGGTA CTGGAGCGTC 
     1801 CCTCATTCCT GCGCAGACCA GTGAATCAGG TGGTCCTGGC TGATGCCCCT GTGACTTTCC 
     1861 TATGTGAGGT GAAGGGGGAT CCCCCACCTC GTCTACGCTG GCGCAAGGAG GATGGGGAAC 
     1921 TGCCCACAGG CAGGTATGAG ATCCGGAGTG ACCACAGCCT TTGGATTGGG CATGTGAGTG 
     1981 CCGAAGATGA GGGAACGTAC ACCTGTGTGG CGGAGAACAG TGTGGGCCGC GCTGAAGCAT 
     2041 CTGGCTCCCT CAGTGTTCAC GTCCCACCCC AGTTGGTGAC CCAGCCCCAG GACCAGATGG 
     2101 CAGCTCCTGG AGAGAGCGTG GCTTTCCAGT GCGAGACCAA AGGAAACCCC CCACCTGCCA 
     2161 TCTTCTGGCA GAAGGAGGGG AGTCAGGTCC TGCTTTTCCC CAGTCAGTCA CTTCAGCCGA 
     2221 CGGGGCGCTT CTCAGTGTCT CCAAGAGGCC AACTTAACAT CACCGCGGTG CAGCGTGGGG 
     2281 ATGCTGGGTA CTACGTGTGC CAGGCTGTCA GTGTGGCTGG CAGCATCCTG GCCAAGGCCC 
     2341 TGCTGGAGAT AAAAGGAGCC TCTTTGGATG GGCTGCCTCC TGTCATCCTC CAGGGACCAG 
     2401 CCAATCAGAC GCTGGTGCTT GGCTCCTCCG TGTGGCTGCC CTGCAGAGTG ACTGGGAACC 
     2461 CTCAACCCAG TGTCCGATGG AAGAAGGATG GGCAGTGGCT GCAGGGGGAT GACCTCCAGT 
     2521 TCAAGACAAT GGCCAACGGT ACCCTGTACA TCGCCAATGT GCAGGAGATG GACATGGGCT 
     2581 TCTACAGCTG CGTGGCCAAG AGTTCCACAG GGGAAGCCAC ATGGAGCGGC TGGCTTAAGA 
     2641 TGCGGGAAGA CTGGGGAGTA TCACCAGACC CCCCTACAGA ACCCAGTTCC CCTCCGGGGG 
     2701 CTCCCTCTCA GCCAGTGGTC ACTGAGATCA CCAAGAACAG CATTACCCTG ACCTGGAAGC 
     2761 CCAACCCACA AACTGGGGCT GCAGTCACGT CTTATGTGAT AGAGGCCTTC AGCCCAGCAG 
     2821 CTGGCAACAC ATGGCGTACT GTGGCAGATG GCGTGCAGCT GGAGACACAC ACAGTCAGCG 
     2881 GTCTGCAGCC CAATACCATC TACCTGTTTC TGGTTCGAGC AGTGGGAGCC TGGGGCCTCA 
     2941 GTGAGCCCAG CCCCGTCTCT GAGCCTGTCC GTACACAGGA TAGCAGCCCC TCTAGGCCAG 
     3001 TGGAGGACCC ATGGAGAGGC CAGCAGGGAC TGGCGGAAGT GGCTGTGCGC CTGCAGGAGC 
     3061 CCATAGTCCT GGGACCCCGG ACCCTGCAGG TGTCCTGGAC TGTGGATGGC CCAGTCCAGC 
     3121 TGGTGCAAGG TTTCCGGGTG TCTTGGAGGG TAGCAGGCCC TGAGGGAGGA AGCTGGACAA 
     3181 TGTTGGACCT ACAGTCCCCA AGCCAGCAAA GTACTGTGCT AAGAGGACTC CCTCCAGGGA 
     3241 CCCAAATCCA GATCAAGGTG CAAGCCCAAG GCCAGGAGGG GCTGGGGGCT GAAAGCCTCT 
     3301 CTGTGACCAG GAGCATTCCT GAGGAGGCCC CCAGTGGCCC CCCACAGGGA GTGGCGGTGG 
     3361 CCTTGGGGGG TGATGGCAAC AGCAGTATCA CTGTGTCCTG GGAACCTCCA CTCCCCTCCC 
     3421 AGCAAAATGG GGTCATCACG GAATACCAGA TCTGGTGCCT GGGCAATGAG AGCCGCTTTC 
     3481 ACCTCAATCG ATCTGCAGCA GGCTGGGCAC GCTCCGCAAT GCTCCGAGGA CTGGTGCCCG 
     3541 GTCTCCTCTA TCGAACCCTG GTCGCGGCGG CCACCAGCGC AGGCGTGGGC GTGCCCAGTG 
     3601 CCCCAGTGCT GGTGCAGCTG CCGTCCCCGC CGGACCTGGA GCCCGGGCTG GAGGTGGGCG 
     3661 CGGGGCTGGC GGTGCGGCTG GCGAGGGTGC TGCGGGAGCC CGCCTTCCTC GCGGGCAGCG 
     3721 GCGCAGCCTG CGGGGCGCTG CTTCTCGGGC TCTGCGCCGC CCTCTACTGG CGCCGGAAAC 
     3781 AGCGCAAAGA GCTCAGCCAC TACACGGCCT CTTTTGCCTA CACACCGGCA GTGTCCTTCC 
     3841 CGCACTCAGA GGGCCTCTCT GGAGCCAGTT CCAGGCCACC CATGGGCCTT GGCCCCGCCC 
     3901 CCTACTCATG GCTGGCAGAT TCGTGGCCCC ACCCATCTCG AAGCCCCTCG GCCCAGGAAC 
     3961 CCAGGGGAAG CTGCTGCCCT AGCAATCCTG ACCCGGACGA CAGATATTAC AACGAAGCGG 
     4021 GAATCTCCCT GTATCTAGCT CAGACGGCCA GGGGCACGGC CGCCCCTGGC GAGGGTCCTG 
     4081 TCTATAGCAC CATTGACCCA GCGGGGGAGG AGCTGCAGAC CTTCCATGGG GGCTTCCCCC 
     4141 AACATCCCTC AGGAGATCTG GGTCCCTGGA GCCAGTACGC TCCTCCAGAG TGGAGCCAGG 
     4201 GGGACAGTGG AGCCAAGGGA GGCAAAGTGA AGCTTCTGGG GAAACCTGTG CAGATGCCCT 
     4261 CTCTGAACTG GCCAGAAGCC CTGCCCCCAC CTCCTCCTTC TTGTGAACTG AGCTGCCTAG 
     4321 AAGGGCCGGA GGAGGAGCTG GAGGGCAGCT CAGAGCCAGA GGAGTGGTGC CCGCCAATGC 
     4381 CTGAGAGAAG TCACCTGACG GAGCCCAGCT CCAGTGGAGG GTGCCTGGTC ACCCCATCCC 
     4441 GAAGGGAAAC CCCCTCTCCC ACACCTTCCT ATGGACAGCA GTCCACAGCC ACTCTTACAC 
     4501 CCTCACCTCC TGACCCTCCC CAGCCCCCAA CTGACATGCC CCATCTCCAT CAGATGCCCA 
     4561 GGAGGGTGCC CCTTGGGCCG AGTTCCCCTC TCAGTGTATC CCAGCCCATG CTGGGCATCC 
     4621 GTGAAGCGAG GCCTGCTGGC TTGGGTGCTG GCCCTGCAGC CTCACCCCAC CTCAGCCCCA 
     4681 GTCCTGCCCC TAGCACAGCC AGCAGTGCCC CAGGCAGAAC CTGGCAGGGG AATGGGGAGA 
     4741 TGACTCCCCC ACTTCAAGGA CCCCGTGCTC GATTCCGGAA GAAACCCAAG GCTCTTCCCT 
     4801 ACAGGAGGGA GAACAGTCCT GGGGACTTGC CCCCACCACC CTTGCCACCG CCAGAGGAAG 
     4861 AGGCGAGCTG GGCCCTAGAG CTGAGGGCAG CAGGCAGCAT GTCCTCCCTG GAGCGGGAGC 
     4921 GCAGTGGGGA GAGGAAAGCG GTCCAGGCCG TGCCCCTGGC AGCCCAGCGG GTGCTCCACC 
     4981 CAGATGAAGA GGCCTGGCTC CCATACAGCA GACCAAGCTT CCTGTCCCGG GGCCAGGGCA 
     5041 CCAGCACATG TTCCACGGCC GGCAGCAACT CTTCCAGGGG CTCCAGCAGC TCTAGGGGCT 
     5101 CCCGGGGCCC TGGCCGGAGC CGGAGTCGGA GTCAGAGCCG GAGCCAGAGC CAAAGGCCAG 
     5161 GACAGAAACG CCGAGAGGAA CCAAGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5221 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5281 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5341 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5401 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5461 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5521 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5581 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5641 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5701 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5761 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5821 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5881 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5941 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6001 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6061 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6121 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6181 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6241 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6301 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6361 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6421 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6481 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6541 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6601 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6661 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6721 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6781 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6841 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6901 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6961 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7021 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7081 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7141 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7201 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7261 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7321 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7381 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7441 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7501 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7561 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7621 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7681 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7741 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7801 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7861 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7921 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7981 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8041 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8101 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8161 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8221 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8281 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8341 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8401 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8461 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8521 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8581 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8641 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8701 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8761 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8821 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8881 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8941 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9001 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//