LOCUS       RC216391                8754 bp    DNA     circular  SYN 
DEFINITION  PLCG1 (Myc-DDK-tagged)-Human phospholipase C, gamma 1 (PLCG1), transcript variant 2
ACCESSION   NM_182811
VERSION     NM_182811.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4911..5002 
                     /label="Myc-DDK"  
     misc_feature    5032..5617 
                     /label="PolyA signal"  
     misc_feature    5089..5108 
                     /label="Restriction Enzyme"  
     misc_feature    5893..6565 
                     /label="ColE1"  
     misc_feature    6995..7789 
                     /label="Kan/Neo"  
     misc_feature    7824..8126 
                     /label="SV40 ori"  
     misc_feature    8212..8689 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4893..4899 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4893 
                     /label="PLCG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGCGCC GCGTCCCCTT GCGCCAACGG CTGCGGGCCC GGCGCGCCCT 
     1081 CGGACGCCGA GGTGCTGCAC CTCTGCCGCA GCCTCGAGGT GGGCACCGTC ATGACTTTGT 
     1141 TCTACTCCAA GAAGTCGCAG CGACCCGAGC GGAAGACCTT CCAGGTCAAG CTGGAGACGC 
     1201 GCCAGATCAC GTGGAGCCGG GGCGCCGACA AGATCGAGGG GGCCATTGAC ATTCGTGAAA 
     1261 TTAAGGAGAT CCGCCCAGGG AAGACCTCAC GGGACTTTGA TCGCTATCAA GAGGACCCAG 
     1321 CTTTCCGGCC GGACCAGTCA CATTGCTTTG TCATTCTCTA TGGAATGGAA TTTCGCCTGA 
     1381 AAACGCTGAG CCTGCAAGCC ACATCTGAGG ATGAAGTGAA CATGTGGATC AAGGGCTTAA 
     1441 CTTGGCTGAT GGAGGATACA TTGCAGGCAC CCACACCCCT GCAGATTGAG AGGTGGCTCC 
     1501 GGAAGCAGTT TTACTCAGTG GATCGGAATC GTGAGGATCG TATATCAGCC AAGGACCTGA 
     1561 AGAACATGCT GTCCCAGGTC AACTACCGGG TCCCCAACAT GCGCTTCCTC CGAGAGCGGC 
     1621 TGACGGACCT GGAGCAGCGC AGCGGGGACA TCACCTACGG GCAGTTTGCT CAGCTGTACC 
     1681 GCAGCCTCAT GTACAGCGCC CAGAAGACGA TGGACCTCCC CTTCTTGGAA GCCAGTACTC 
     1741 TGAGGGCTGG GGAGCGGCCG GAGCTTTGCC GAGTGTCCCT TCCTGAGTTC CAGCAGTTCC 
     1801 TTCTTGACTA CCAGGGGGAG CTGTGGGCTG TTGATCGCCT CCAGGTGCAG GAGTTCATGC 
     1861 TCAGCTTCCT CCGAGACCCC TTACGAGAGA TCGAGGAGCC ATACTTCTTC CTGGATGAGT 
     1921 TTGTCACCTT CCTGTTCTCC AAAGAGAACA GTGTGTGGAA CTCGCAGCTG GATGCAGTAT 
     1981 GCCCGGACAC CATGAACAAC CCTCTTTCCC ACTACTGGAT CTCCTCCTCG CACAACACGT 
     2041 ACCTGACCGG GGACCAGTTC TCCAGTGAGT CCTCCTTGGA AGCCTATGCT CGCTGCCTGC 
     2101 GGATGGGCTG TCGCTGCATT GAGTTGGACT GCTGGGACGG CCCGGATGGG ATGCCAGTTA 
     2161 TTTACCATGG GCACACCCTT ACCACCAAGA TCAAGTTCTC AGATGTCCTG CACACCATCA 
     2221 AGGAGCATGC CTTTGTGGCC TCAGAGTACC CAGTCATCCT GTCCATTGAG GACCACTGCA 
     2281 GCATTGCCCA GCAGAGAAAC ATGGCCCAAT ACTTCAAGAA GGTGCTGGGG GACACACTCC 
     2341 TCACCAAGCC CGTGGAGATC TCTGCCGACG GGCTCCCCTC ACCCAACCAG CTTAAGAGGA 
     2401 AGATCCTCAT CAAGCACAAG AAGCTGGCTG AGGGCAGTGC CTACGAGGAG GTGCCTACAT 
     2461 CCATGATGTA CTCTGAGAAC GACATCAGCA ACTCTATCAA GAATGGCATC CTCTACCTGG 
     2521 AGGACCCTGT GAACCACGAA TGGTATCCCC ACTACTTTGT TCTGACCAGC AGCAAGATCT 
     2581 ACTACTCTGA GGAGACCAGC AGTGACCAGG GCAACGAGGA TGAGGAGGAG CCCAAGGAGG 
     2641 TCAGCAGCAG CACAGAGCTG CACTCCAATG AGAAGTGGTT CCATGGGAAG CTAGGGGCAG 
     2701 GGCGTGACGG GCGTCACATC GCTGAGCGCC TGCTTACTGA GTACTGCATC GAGACCGGAG 
     2761 CCCCTGACGG CTCCTTCCTC GTGCGAGAGA GTGAGACCTT CGTGGGCGAC TACACGCTCT 
     2821 CTTTCTGGCG GAACGGGAAA GTCCAGCACT GCCGTATCCA CTCCCGGCAA GATGCTGGGA 
     2881 CCCCCAAGTT CTTCTTGACA GACAACCTCG TCTTTGACTC CCTCTATGAC CTCATCACGC 
     2941 ACTACCAGCA GGTGCCCCTG CGCTGTAATG AGTTTGAGAT GCGACTTTCA GAGCCTGTCC 
     3001 CACAGACCAA CGCCCACGAG AGCAAAGAGT GGTACCACGC GAGCCTGACC AGAGCACAGG 
     3061 CTGAGCACAT GCTAATGCGC GTCCCTCGTG ATGGGGCCTT CCTGGTGCGG AAGCGGAATG 
     3121 AACCCAACTC ATATGCCATC TCTTTCCGGG CTGAGGGCAA GATCAAGCAT TGCCGTGTCC 
     3181 AGCAAGAGGG CCAGACAGTG ATGCTAGGGA ACTCGGAGTT CGACAGCCTT GTTGACCTCA 
     3241 TCAGCTACTA TGAGAAACAC CCGCTATACC GCAAGATGAA GCTGCGCTAT CCCATCAACG 
     3301 AGGAGGCACT GGAGAAGATT GGCACAGCTG AGCCTGACTA CGGGGCCCTG TATGAGGGAC 
     3361 GCAACCCTGG CTTCTATGTA GAGGCAAACC CTATGCCAAC TTTCAAGTGT GCAGTCAAAG 
     3421 CCCTCTTTGA CTACAAGGCC CAGAGGGAGG ACGAGCTGAC CTTCATCAAG AGCGCCATCA 
     3481 TCCAGAATGT GGAGAAGCAA GAGGGAGGCT GGTGGCGAGG GGACTACGGA GGGAAGAAGC 
     3541 AGCTGTGGTT CCCATCAAAC TACGTGGAAG AGATGGTCAA CCCCGTGGCC CTGGAGCCGG 
     3601 AGAGGGAGCA CTTGGACGAG AACAGCCCCC TAGGGGACTT GCTGCGGGGG GTCTTGGATG 
     3661 TGCCGGCTTG TCAGATTGCC ATCCGTCCTG AGGGCAAGAA CAACCGGCTC TTCGTCTTCT 
     3721 CCATCAGCAT GGCGTCGGTG GCCCACTGGT CCCTGGATGT TGCTGCCGAC TCACAGGAGG 
     3781 AGCTGCAGGA CTGGGTGAAA AAGATCCGTG AAGTGGCCCA GACAGCAGAC GCCAGGCTCA 
     3841 CTGAAGGGAA GATAATGGAA CGGAGGAAGA AGATTGCCCT GGAGCTCTCT GAACTTGTCG 
     3901 TCTACTGCCG GCCTGTTCCC TTTGATGAAG AGAAGATTGG CACAGAACGT GCTTGCTACC 
     3961 GGGACATGTC ATCCTTCCCG GAAACCAAGG CTGAGAAATA CGTGAACAAG GCCAAAGGCA 
     4021 AGAAGTTCCT TCAGTACAAT CGACTGCAGC TCTCCCGCAT CTACCCCAAG GGCCAGCGAC 
     4081 TGGATTCCTC CAACTACGAT CCTTTGCCCA TGTGGATCTG TGGCAGTCAG CTTGTGGCCC 
     4141 TCAACTTCCA GACCCCTGAC AAGCCTATGC AGATGAACCA GGCCCTCTTC ATGACGGGCA 
     4201 GGCACTGTGG CTACGTGCTG CAGCCAAGCA CCATGCGGGA TGAGGCCTTC GACCCCTTTG 
     4261 ACAAGAGCAG CCTCCGCGGG CTGGAGCCAT GTGCCATCTC TATTGAGGTG CTGGGGGCCC 
     4321 GACATCTGCC AAAGAATGGC CGAGGCATTG TGTGTCCTTT TGTGGAGATT GAGGTGGCTG 
     4381 GAGCTGAGTA TGACAGCACC AAGCAGAAGA CAGAGTTTGT GGTGGACAAT GGACTCAACC 
     4441 CTGTATGGCC AGCCAAGCCC TTCCACTTCC AGATCAGTAA CCCTGAATTT GCCTTTCTGC 
     4501 GCTTCGTGGT GTATGAGGAA GACATGTTTA GTGACCAGAA TTTCCTGGCT CAGGCTACTT 
     4561 TCCCAGTAAA AGGCCTGAAG ACAGGATACA GAGCAGTGCC TTTGAAGAAC AACTACAGTG 
     4621 AGGACCTGGA GTTGGCCTCC CTGCTGATCA AGATTGACAT TTTCCCTGCC AAGCAGGAGA 
     4681 ATGGTGACCT CAGTCCCTTC AGTGGTACGT CCCTGCGGGA GCGGGGCTCA GATGCCTCAG 
     4741 GCCAGCTGTT TCATGGCCGA GCCCGGGAAG GCTCCTTTGA ATCCCGCTAC CAGCAGCCGT 
     4801 TTGAGGACTT CCGCATCTCC CAGGAGCATC TCGCAGACCA TTTTGACAGT CGAGAACGAA 
     4861 GGGCCCCAAG AAGGACTCGG GTCAATGGAG ACAACCGCCT CACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//