LOCUS       RC216388                7950 bp    DNA     circular  SYN 
DEFINITION  CDCA2 (Myc-DDK-tagged)-Human cell division cycle associated 2 (CDCA2)
ACCESSION   NM_152562
VERSION     NM_152562.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4107..4198 
                     /label="Myc-DDK"  
     misc_feature    4228..4813 
                     /label="PolyA signal"  
     misc_feature    4285..4304 
                     /label="Restriction Enzyme"  
     misc_feature    5089..5761 
                     /label="ColE1"  
     misc_feature    6191..6985 
                     /label="Kan/Neo"  
     misc_feature    7020..7322 
                     /label="SV40 ori"  
     misc_feature    7408..7885 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4089..4095 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4089 
                     /label="CDCA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCCAAT TCAAAAGACA AGCCCCCTGA AACCAAGGAG TCTGCAATGA 
     1081 ATAATGCTGG AAATGCCTCT TTCATTTTGG GAACTGGGAA GATTGTGACT CCTCAGAAGC 
     1141 ATGCCGAATT ACCTCCTAAT CCTTGCACAC CAGATACTTT TAAATCACCT TTGAACTTTT 
     1201 CCACAGTAAC CGTAGAGCAA TTGGGAATTA CACCTGAAAG CTTTGTTAGG AACTCTGCAG 
     1261 GAAAGTCATC ATCCTACCTT AAAAAATGTA GACGACGTTC TGCAGTCGGT GCTCGGGGCT 
     1321 CTCCTGAAAC AAACCATCTG ATTCGTTTCA TTGCTCGGCA GCAAAATATA AAGAATGCTA 
     1381 GGAAATCTCC TTTGGCACAA GATTCTCCTT CCCAGGGCAG CCCTGCACTG TATCGAAATG 
     1441 TTAACACTTT AAGAGAACGA ATATCAGCCT TCCAGTCAGC TTTTCACTCC ATAAAGGAAA 
     1501 ACGAGAAAAT GACCGGCTGT CTGGAATTCT CAGAGGCAGG AAAAGAGTCC GAGATGACAG 
     1561 ACTTGACCAG AAAGGAAGGT CTCAGCGCTT GCCAGCAGTC TGGGTTCCCT GCAGTGTTGT 
     1621 CCTCCAAACG TCGGAGAATA TCCTATCAGA GAGACTCTGA TGAAAATCTG ACGGATGCTG 
     1681 AAGGAAAAGT AATTGGTCTC CAGATATTCA ATATTGATAC AGACAGAGCA TGTGCAGTTG 
     1741 AAACTTCTGT AGATCTTTCT GAGATATCAT CTAAACTTGG TTCAACACAG TCTGGATTTT 
     1801 TAGTTGAAGA GTCTCTTCCC CTTTCAGAGC TCACAGAGAC TTCAAATGCA CTAAAGGTTG 
     1861 CTGACTGTGT AGTGGGCAAA GGATCAAGTG ATGCCGTTTC GCCTGACACG TTCACAGCAG 
     1921 AAGTGAGCTC AGACGCAGTC CCTGATGTCA GGTCACCAGC TACTCCAGCC TGCAGGAGGG 
     1981 ACCTTCCCAC CCCCAAGACC TTTGTACTTC GTTCTGTACT GAAGAAACCC TCTGTTAAGA 
     2041 TGTGTCTAGA GAGCTTACAG GAACACTGTA ACAACCTCTA TGATGATGAT GGGACTCATC 
     2101 CGAGCTTAAT CTCAAATCTC CCAAACTGTT GCAAAGAGAA AGAAGCAGAA GATGAAGAAA 
     2161 ATTTTGAAGC ACCTGCCTTT CTAAATATGA GGAAGAGGAA GAGAGTTACT TTTGGAGAGG 
     2221 ACTTAAGCCC GGAAGTGTTT GATGAATCTT TGCCAGCAAA TACTCCATTG CGTAAAGGAG 
     2281 GAACACCTGT TTGTAAAAAA GACTTCAGTG GTCTCAGTTC CCTGCTGCTT GAGCAGTCAC 
     2341 CTGTTCCTGA GCCATTACCT CAACCAGATT TTGATGACAA GGGGGAGAAT CTTGAAAACA 
     2401 TAGAACCACT TCAAGTATCA TTTGCCGTTC TCAGTTCTCC TAATAAATCA TCAATCTCTG 
     2461 AGACCCTTTC AGGCACTGAT ACCTTTAGTT CTTCAAATAA CCATGAGAAA ATATCCTCTC 
     2521 CTAAAGTTGG TAGAATAACA AGGACTTCTA ACAGAAGAAA TCAATTGGTC AGTGTTGTAG 
     2581 AAGAGAGTGT TTGCAACTTA TTGAATACAG AAGTTCAGCC TTGTAAAGAA AAGAAAATTA 
     2641 ATAGGAGGAA GTCTCAAGAA ACAAAGTGTA CAAAGAGAGC ACTTCCTAAG AAGAGTCAGG 
     2701 TTTTAAAAAG TTGCAGAAAG AAGAAAGGAA AGGGAAAGAA AAGTGTTCAG AAATCTTTAT 
     2761 ATGGGGAAAG AGACATTGCT TCTAAGAAGC CCCTCCTCAG TCCTATTCCC GAGCTGCCTG 
     2821 AAGTCCCTGA GATGACACCT TCCATTCCGA GCATCCGAAG ACTGGGTTCA GGTTATTTCA 
     2881 GTTCAAATGG CAAACTGGAA GAAGTGAAGA CTCCTAAAAA TCCAGTGAAA AGAAAGGATC 
     2941 TTTTGCGTCA TGACCCAGAT TTGCATATGC ATCAAGGCTA TGATAAATAT GATGTCTCTG 
     3001 AATTCTGCTC TTATATAAAA AGTTCCTCAT CGCTTGGCAA TGCTACTTCT GATGAAGATC 
     3061 CAAATACAAA TATAATGAAC ATTAATGAAA ATAAAAATAT TCCAAAAGCA AAAAATAAGT 
     3121 CAGAAAGTGA AAATGAACCA AAAGCTGGAA CTGACAGTCC TGTTTCTTGT GCTTCTGTAA 
     3181 CTGAAGAACG TGTGGCATCA GATAGTCCCA AACCTGCTCT GACCCTGCAG CAGGGTCAAG 
     3241 AATTTTCTGC TGGTGGTCAA AATGCAGAAA ACCTTTGTCA GTTCTTTAAA ATTTCACCAG 
     3301 ATTTAAACAT AAAGTGTGAA AGAAAGGATG ACTTCTTAGG AGCTGCAGAA GGAAAACTGC 
     3361 AATGCAATCG TTTAATGCCT AATTCACAAA AAGACTGTCA TTGTTTAGGA GATGTCTTAA 
     3421 TTGAAAATAC GAAAGAATCT AAAAGCCAGA GTGAGGATTT GGGAAGAAAA CCCATGGAAA 
     3481 GTAGCAGTGT TGTGAGTTGC AGAGACAGGA AAGATAGAAG ACGTTCCATG TGTTATTCTG 
     3541 ATGGTCGAAG TTTACATTTG GAAAAAAATG GAAATCACAC ACCATCCTCC AGTGTGGGCA 
     3601 GCTCTGTAGA AATTAGTTTA GAAAATTCTG AACTGTTTAA AGATTTGTCT GATGCCATTG 
     3661 AGCAAACCTT TCAGAGGAGA AATAGTGAAA CCAAAGTGCG ACGTAGCACG AGGCTACAGA 
     3721 AGGATTTAGA AAACGAAGGT CTTGTATGGA TTTCACTTCC ACTTCCTTCC ACTTCCCAAA 
     3781 AAGCCAAAAG AAGAACAATA TGTACATTTG ACAGCAGTGG ATTTGAAAGT ATGTCTCCCA 
     3841 TAAAAGAAAC TGTGTCCTCC AGACAAAAAC CGCAGATGGC ACCTCCCGTC TCAGATCCAG 
     3901 AAAACAGCCA GGGCCCTGCT GCTGGTTCTT CCGATGAACC TGGTAAGAGG AGGAAGAGCT 
     3961 TTTGTATATC TACACTTGCA AATACTAAAG CCACTTCCCA GTTCAAAGGC TACCGGAGAA 
     4021 GATCCTCTCT TAATGGGAAG GGAGAGAGCT CTCTGACTGC CTTGGAAAGG ATTGAACATA 
     4081 ATGGAGAAAG AAAGCAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4141 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4201 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4261 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4321 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4381 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4441 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4501 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4561 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4621 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4681 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4741 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4801 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4861 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4921 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4981 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5041 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5101 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5161 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5221 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5281 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5341 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5401 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5461 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5521 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5581 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5641 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5701 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5761 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5821 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5881 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5941 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6001 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6061 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6121 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6181 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6241 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6301 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6361 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6421 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6481 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6541 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6601 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6661 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6721 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6781 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6841 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6901 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6961 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7021 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7081 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7141 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7201 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7261 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7321 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7381 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7441 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7501 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7561 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7621 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7681 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7741 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7801 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7861 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7921 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//