LOCUS       RC216347                7005 bp    DNA     circular  SYN 
DEFINITION  FBXO43 (Myc-DDK-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2
ACCESSION   NM_001029860
VERSION     NM_001029860.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3162..3253 
                     /label="Myc-DDK"  
     misc_feature    3283..3868 
                     /label="PolyA signal"  
     misc_feature    3340..3359 
                     /label="Restriction Enzyme"  
     misc_feature    4144..4816 
                     /label="ColE1"  
     misc_feature    5246..6040 
                     /label="Kan/Neo"  
     misc_feature    6075..6377 
                     /label="SV40 ori"  
     misc_feature    6463..6940 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3144..3150 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3144 
                     /label="FBXO43"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTTAAA GACAAAGATG AGAGAATTTC TTGTTTGGAA GCCTACGTAA 
     1081 CTTTGACATC TAAGAGCTCA AGATTTACTG ATGAAACAGA GATTTTGAAG ATGTCGCAAA 
     1141 GGCACTCAGG TCAAGCTGGC ACTGAAGCAG GAAATGGGGC GGACTCTCCT CCAATTGTCA 
     1201 ACTCCAAGTA CTCCACCTTC AGAGATTTTT GTTCCACATC TTCATTTCAA GATAGTGGCT 
     1261 ACAATGAGTT AAAATCTTGT AGCTTTGATA ATATAGATAA AGAATATCTT GGAAAGAAAG 
     1321 AAAAAGGCCC AACATTACTC TATGAGCACC CTGAAACTTC AGGCCTGGGC TTAACACATC 
     1381 CTTTAGAATC TCCCACTCAA AAAAAGAAAT GTATCTTGCC TAGAAAGGAA AAGGATAAAA 
     1441 CCCCAGAACT TTGTGAAACA CCTAAAATCA GTGGGAAAAA ATGTTTACCT CGCAGAAGGT 
     1501 TGAATGTATC TTTCGCTCTT CTAAAAGGGG ACTTTGAATC ACAAAATAGT TCTTTAGAAA 
     1561 GTAGTATAAG CCAAGTTATC AACTTAGAAA AAAATATTCC AAGCAGTGCT TCAGGTTTTT 
     1621 CCAGGGCAAA TAATTTTAGC CCTTTAGTTA CTAGCACTTT AAAAACAGAA GAAGTGACTT 
     1681 CATGCAGTCA AAAATTGAGG CTTAATTTTT CTCAGCAAAA GACTTCCACA ATTGATGATT 
     1741 CCAAAGATGA TTGTAGCCTA TTTGAAGTTG AATGTATATC TCCAATTCAG GGCAATAATT 
     1801 TTAAAGACTC TATCACACAT GACTTTAGTG ATAGCAGTTT ATGCATTAAT GATGAGAATG 
     1861 CATGTCCAGA GCTCCTGGGC TCCTCTGTTA GTGGAACAAC TTGTGGAACA GATGAGGACA 
     1921 TATTTGTGAC TCCGATAAGT AATCTTGTGG CAAACATTAG ATTTAACGCA AGTCAAATAC 
     1981 TTTCTCCTTC ACCTGAAGTG AGAGGCAGTA TTTCAACGCC TGAAGACAGT GGTTTTAACT 
     2041 CACTTAGCTT GGAGAAATCA GAAGATTCCC TGTCTGACCA GGAGGGTTCT TTTCAAGAAC 
     2101 TACTGCAGAA ACATAAGGGG ACTCCCAAAG TTGGGGACAC CATAAGAAAG ACAAGACATC 
     2161 TTGGAAGGTC GAGAAGACTG TCCACCCTTC GGGAACAAAG CTCGCAGTCA GAGACAGAAG 
     2221 AGGAAAAGCA GATTGTCCAC CCTGACTCTG AAAAAAGAGC AGCAGCTGCT TCTGCCATCT 
     2281 CAGAGGGTCA GCTGAGTAGT GATGAGAGTG GGGATTTGAC CTTTAGCTTA AAGAATTTAT 
     2341 CAAAGACCCC AGCCTTGCAA TTGGTACATG AGCTGTTCAT GAAAAGCAAG AGGAAAAGAT 
     2401 TACAGGAAAA TAGTGGACAT GAATTCTTAG AGCAAGGGGA TGGGGAGAAA ATAGCTGTAC 
     2461 TGCAGTGTAT ACTTGCAGGA CTGATCGGCA AGAAAATGGG TATAGAAAAA CTGGACATCT 
     2521 TAACAGAATT AAAATATAGA AATTTAAAGC ATATTCTTGC TATGGTTTTA GAGTCCTTGA 
     2581 CCGCAGAGAG CCTATGCAGT GTTTGGAAAG TAAGCAGAAA TTGGCGTGAA ATTGTTGTTC 
     2641 AAGATAAAAA TGCAAATCGG AGGAGGAAAT TTTATATCAC ACAACTGAAA ACAGATTCTG 
     2701 AGGGGGCTGT ATTAAATGTC GAGGATGCTG CCACTCGGCT CCAGCTTTTA AATCGCTCAG 
     2761 CTTTAAGATC TGTGCAGGCA CAGGCTAGGA TACCTGGTTC TCAGAGAGAG CAAGGGTCAA 
     2821 CATTATCTCC CTGGGGAGAA GTTTTGACAC CTCTAGCAAG CTCTTCTGTT ACTCACTTAA 
     2881 GTAGTAAACA GGAAGAATAT GTTAAGGTTG CCAAAACACT TTTTACTGAT GAAGCATTAA 
     2941 AACCTTGCCC AAGGTGCCAG TCCCCTGCTA AGTACCAGCC ATATAAGAAA AGGGGACTGT 
     3001 GTAGCCGAAC AGCCTGTGGT TTTGACTTTT GTGTGTTATG TCTGTGTGCT TATCATGGGT 
     3061 CTGAAGAATG TAGTAGAGGA GCAGCAAAGC CAAGAAATAG AAAAGATGCT CTCCCAGGAA 
     3121 GTGCCCAGAG TAAGCGGAAT TTAAAACGCC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3181 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3241 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3301 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3361 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3421 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3481 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3541 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3601 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3661 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3721 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3781 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3841 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3901 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3961 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4021 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4081 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4141 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4201 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4261 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4321 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4381 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4441 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4501 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4561 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4621 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4681 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4741 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4801 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4861 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4921 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4981 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5041 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5101 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5161 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5221 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5281 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5341 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5401 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5461 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5521 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5581 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5641 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5701 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5761 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5821 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5881 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5941 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6001 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6061 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6121 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6181 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6241 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6301 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6361 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6421 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6481 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6541 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6601 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6661 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6721 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6781 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6841 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6901 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6961 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//