LOCUS       RC216330L1                9048 bp    DNA     circular  SYN 
DEFINITION  SLFN5 (Myc-DDK-tagged)-Human schlafen family member 5 (SLFN5)
ACCESSION   NM_144975
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7339..7519 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8247..8906 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7678..8246 
                     /label="ColE1"  
     misc_feature    6214..6234 
                     /label="LR50 primer"  
     misc_feature    6011..6082 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6223..6814 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5993..5999 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5993 
                     /label="SLFN5"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGTCTTAGG ATTGATGTGG ATACAAACTT 
     3361 TCCTGAGTGT GTTGTAGATG CAGGAAAAGT CACCCTTGGG ACTCAGCAGA GGCAGGAGAT 
     3421 GGACCCTCGC CTGCGGGAGA AACAGAATGA AATCATCCTG CGAGCAGTAT GTGCTCTGCT 
     3481 GAATTCTGGT GGGGGCATAA TCAAGGCTGA GATTGAGAAC AAAGGCTACA ATTATGAACG 
     3541 TCATGGAGTA GGATTGGATG TGCCTCCAAT TTTCAGAAGC CATTTAGATA AGATGCAGAA 
     3601 GGAAAACCAC TTTTTGATTT TTGTGAAATC ATGGAACACA GAGGCTGGTG TGCCACTTGC 
     3661 TACCTTATGC TCCAATTTGT ACCACAGAGA GAGAACATCC ACCGATGTCA TGGATTCTCA 
     3721 GGAAGCTCTG GCATTCCTCA AATGCAGGAC TCAGACTCCA ACGAATATTA ATGTTTCCAA 
     3781 TTCATTAGGT CCACAGGCAG CTCAGGGTAG TGTACAATAT GAAGGTAACA TAAATGTGTC 
     3841 AGCTGCTGCT TTATTTGATA GAAAGCGGCT TCAGCATCTG GAAAAACTCA ACCTTCCTGA 
     3901 GTCCACACAT GTTGAATTTG TAATGTTCTC GACAGACGTG TCACACTGTG TTAAAGACAG 
     3961 ACTTCCGAAG TGTGTTTCTG CATTTGCAAA TACTGAAGGA GGATATGTAT TTTTTGGTGT 
     4021 GCATGATGAG ACTTGTCAAG TGATTGGATG TGAAAAAGAG AAAATAGACC TTACGAGCTT 
     4081 GAGGGCTTCT ATTGATGGCT GTATTAAGAA GCTACCTGTC CATCATTTCT GCACACAGAG 
     4141 GCCTGAGATA AAATATGTCC TTAACTTCCT TGAAGTGCAT GATAAGGGGG CCCTCCGTGG 
     4201 ATATGTCTGT GCAATCAAGG TGGAGAAATT CTGCTGTGCG GTGTTTGCCA AAGTGCCTAG 
     4261 TTCCTGGCAG GTGAAGGACA ACCGTGTGAG ACAATTGCCC ACAAGAGAAT GGACTGCTTG 
     4321 GATGATGGAA GCTGACCCAG ACCTTTCCAG GTGTCCTGAG ATGGTTCTCC AGTTGAGTTT 
     4381 GTCATCTGCC ACGCCCCGCA GCAAGCCTGT GTGCATTCAT AAGAATTCGG AATGTCTGAA 
     4441 AGAGCAGCAG AAACGCTACT TTCCAGTATT TTCAGACAGA GTGGTATATA CTCCAGAAAG 
     4501 CCTCTACAAG GAACTCTTCT CACAACATAA AGGACTCAGA GACTTAATAA ATACAGAAAT 
     4561 GCGCCCTTTC TCTCAAGGAA TATTGATTTT TTCTCAAAGC TGGGCTGTGG ATTTAGGTCT 
     4621 GCAAGAGAAG CAGGGAGTCA TCTGTGATGC TCTTCTAATT TCCCAGAACA ACACCCCTAT 
     4681 TCTCTACACC ATCTTCAGCA AGTGGGATGC GGGGTGCAAG GGCTATTCTA TGATAGTTGC 
     4741 CTATTCTTTG AAGCAGAAGC TGGTGAACAA AGGCGGCTAC ACTGGGAGGT TATGCATCAC 
     4801 CCCCTTGGTC TGTGTGCTGA ATTCTGATAG AAAAGCACAG AGCGTTTACA GTTCGTATTT 
     4861 ACAAATTTAC CCTGAATCCT ATAACTTCAT GACCCCCCAG CACATGGAAG CCCTGTTACA 
     4921 GTCCCTCGTG ATAGTCTTGC TTGGGTTCAA ATCCTTCTTA AGTGAAGAGC TGGGCTCTGA 
     4981 GGTTTTGAAC CTACTGACAA ATAAACAGTA TGAGTTGCTT TCAAAGAACC TTCGCAAGAC 
     5041 CAGAGAGTTG TTTGTTCATG GCTTACCTGG ATCAGGGAAG ACTATCTTGG CTCTTAGGAT 
     5101 CATGGAGAAG ATCAGGAATG TGTTTCACTG TGAACCGGCT AACATTCTCT ACATCTGTGA 
     5161 AAACCAGCCC CTGAAGAAGT TGGTGAGTTT CAGCAAGAAA AACATCTGCC AGCCAGTGAC 
     5221 CCGGAAAACC TTCATGAAAA ACAACTTTGA ACACATCCAG CACATTATCA TTGATGACGC 
     5281 TCAGAATTTC CGTACTGAAG ATGGGGACTG GTATGGGAAA GCAAAGTTCA TCACTCAGAC 
     5341 AGCAAGGGAT GGCCCAGGAG TTCTCTGGAT CTTTCTGGAC TACTTTCAGA CCTATCACTT 
     5401 GAGTTGCAGT GGCCTCCCCC CTCCCTCAGA CCAGTATCCA AGAGAAGAGA TCAACAGAGT 
     5461 GGTCCGCAAT GCAGGTCCAA TAGCTAATTA CCTACAACAA GTAATGCAGG AAGCCCGACA 
     5521 AAATCCTCCA CCTAACCTCC CCCCTGGGTC CCTGGTGATG CTCTATGAAC CTAAATGGGC 
     5581 TCAAGGTGTC CCAGGCAACT TAGAGATTAT TGAAGACTTG AACTTGGAGG AGATACTGAT 
     5641 CTATGTAGCG AATAAATGCC GTTTTCTCTT GCGGAATGGT TATTCTCTGA AGGATATTGC 
     5701 TGTGCTTTTC ACCAAAGCAA GTGAAGTGGA AAAATATAAA GACAGGCTTC TAACAGCAAT 
     5761 GAGGAAGAGA AAACTGTCTC AGCTCCATGA GGAGTCTGAT CTGTTACTAC AGATCGGTGA 
     5821 TGCGTCGGAT GTTCTAACCG ATCACATTGT GTTGGACAGT GTCTGTCGAT TTTCAGGCCT 
     5881 GGAAAGAAAT ATCGTGTTTG GAATCAATCC AGGAGTAGCC CCACCGGCTG GGGCCTACAA 
     5941 TCTTCTGCTC TGTTTGGCTT CTAGGGCAAA AAGACATCTG TATATTCTGA AGGCTTCTGT 
     6001 GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     6061 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6121 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6181 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6241 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6301 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6361 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6421 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6481 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     6541 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     6601 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     6661 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     6721 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     6781 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     6841 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     6901 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     6961 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     7021 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     7081 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7141 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7201 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7261 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7321 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7381 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7441 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7501 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     7561 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     7621 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     7681 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     7741 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     7801 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     7861 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     7921 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     7981 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     8041 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     8101 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8161 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8221 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8281 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8341 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8401 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8461 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     8521 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     8581 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     8641 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     8701 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     8761 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     8821 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     8881 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     8941 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9001 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//