LOCUS       RC216326                7680 bp    DNA     circular  SYN 
DEFINITION  DGKZ (Myc-DDK-tagged)-Human diacylglycerol kinase, zeta (DGKZ), transcript variant 3
ACCESSION   NM_201533
VERSION     NM_201533.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3837..3928 
                     /label="Myc-DDK"  
     misc_feature    3958..4543 
                     /label="PolyA signal"  
     misc_feature    4015..4034 
                     /label="Restriction Enzyme"  
     misc_feature    4819..5491 
                     /label="ColE1"  
     misc_feature    5921..6715 
                     /label="Kan/Neo"  
     misc_feature    6750..7052 
                     /label="SV40 ori"  
     misc_feature    7138..7615 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3819..3825 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3819 
                     /label="DGKZ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGCACCG GGGGCTGGCC ACAGTGCCGG GGGAAGCTGC AATGAATCCT 
     1081 CAGCTCTGGG CCCAGTGGAG GCGCTGGGGA CGGAAGAAGG GGAGCGGCCG GGGTCACTGA 
     1141 GGCAGATGTG GCGCTACCGC TCCTGGGACG TGCCACAGAT CCCATCAGAG GCACCCCAGA 
     1201 CACAGAAAGC CATCACCAAG TCGGGCCTCC AGCACCTGGC CCCCCCTCCG CCCACCCCTG 
     1261 GGGCCCCGTG CAGCGAGTCA GAGCGGCAGA TCCGGAGTAC AGTGGACTGG AGCTCAGCGA 
     1321 CATATGGGGA GCACATCTGG TTCGAGACCA ACGTGTCCGG GGACTTCTGC TACGTTGGGG 
     1381 AGCAGTACTG TGTAGCCAGG ATGCTGCAGA AGTCAGTGTC TCGAAGAAAG TGCGCAGCCT 
     1441 GCAAGATTGT GGTGCACACG CCCTGCATCG AGCAGCTGGA GAAGATAAAT TTCCGCTGTA 
     1501 AGCCGTCCTT CCGTGAATCA GGCTCCAGGA ATGTCCGCGA GCCAACCTTT GTACGGCACC 
     1561 ACTGGGTACA CAGACGACGC CAGGACGGCA AGTGTCGGCA CTGTGGGAAG GGATTCCAGC 
     1621 AGAAGTTCAC CTTCCACAGC AAGGAGATTG TGGCCATCAG CTGCTCGTGG TGCAAGCAGG 
     1681 CATACCACAG CAAGGTGTCC TGCTTCATGC TGCAGCAGAT CGAGGAGCCG TGCTCGCTGG 
     1741 GGGTCCACGC AGCCGTGGTC ATCCCGCCCA CCTGGATCCT CCGCGCCCGG AGGCCCCAGA 
     1801 ATACTCTGAA AGCAAGCAAG AAGAAGAAGA GGGCATCCTT CAAGAGGAAG TCCAGCAAGA 
     1861 AAGGGCCTGA GGAGGGCCGC TGGAGACCCT TCATCATCAG GCCCACCCCC TCCCCGCTCA 
     1921 TGAAGCCCCT GCTGGTGTTT GTGAACCCCA AGAGTGGGGG CAACCAGGGT GCAAAGATCA 
     1981 TCCAGTCTTT CCTCTGGTAT CTCAATCCCC GACAAGTCTT CGACCTGAGC CAGGGAGGGC 
     2041 CCAAGGAGGC GCTGGAGATG TACCGCAAAG TGCACAACCT GCGGATCCTG GCGTGCGGGG 
     2101 GCGACGGCAC GGTGGGCTGG ATCCTCTCCA CCCTGGACCA GCTACGCCTG AAGCCGCCAC 
     2161 CCCCTGTTGC CATCCTGCCC CTGGGTACTG GCAACGACTT GGCCCGAACC CTCAACTGGG 
     2221 GTGGGGGCTA CACAGATGAG CCTGTGTCCA AGATCCTCTC CCACGTGGAG GAGGGGAACG 
     2281 TGGTACAGCT GGACCGCTGG GACCTCCACG CTGAGCCCAA CCCCGAGGCA GGGCCTGAGG 
     2341 ACCGAGATGA AGGCGCCACC GACCGGTTGC CCCTGGATGT CTTCAACAAC TACTTCAGCC 
     2401 TGGGCTTTGA CGCCCACGTC ACCCTGGAGT TCCACGAGTC TCGAGAGGCC AACCCAGAGA 
     2461 AATTCAACAG CCGCTTTCGG AATAAGATGT TCTACGCCGG GACAGCTTTC TCTGACTTCC 
     2521 TGATGGGCAG CTCCAAGGAC CTGGCCAAGC ACATCCGAGT GGTGTGTGAT GGAATGGACT 
     2581 TGACTCCCAA GATCCAGGAC CTGAAACCCC AGTGTGTTGT TTTCCTGAAC ATCCCCAGGT 
     2641 ACTGTGCGGG CACCATGCCC TGGGGCCACC CTGGGGAGCA CCACGACTTT GAGCCCCAGC 
     2701 GGCATGACGA CGGCTACCTC GAGGTCATTG GCTTCACCAT GACGTCGTTG GCCGCGCTGC 
     2761 AGGTGGGCGG ACACGGCGAG CGGCTGACGC AGTGTCGCGA GGTGGTGCTC ACCACATCCA 
     2821 AGGCCATCCC GGTGCAGGTG GATGGCGAGC CCTGCAAGCT TGCAGCCTCA CGCATCCGCA 
     2881 TCGCCCTGCG CAACCAGGCC ACCATGGTGC AGAAGGCCAA GCGGCGGAGC GCCGCCCCCC 
     2941 TGCACAGCGA CCAGCAGCCG GTGCCAGAGC AGTTGCGCAT CCAGGTGAGT CGCGTCAGCA 
     3001 TGCACGACTA TGAGGCCCTG CACTACGACA AGGAGCAGCT CAAGGAGGCC TCTGTGCCGC 
     3061 TGGGCACTGT GGTGGTCCCA GGAGACAGTG ACCTAGAGCT CTGCCGTGCC CACATTGAGA 
     3121 GACTCCAGCA GGAGCCCGAT GGTGCTGGAG CCAAGTCCCC GACATGCCAG AAACTGTCCC 
     3181 CCAAGTGGTG CTTCCTGGAC GCCACCACTG CCAGCCGCTT CTACAGGATC GACCGAGCCC 
     3241 AGGAGCACCT CAACTATGTG ACTGAGATCG CACAGGATGA GATTTATATC CTGGACCCTG 
     3301 AGCTGCTGGG GGCATCGGCC CGGCCTGACC TCCCAACCCC CACTTCCCCT CTCCCCACCT 
     3361 CACCCTGCTC ACCCACGCCC CGGTCACTGC AAGGGGATGC TGCACCCCCT CAAGGTGAAG 
     3421 AGCTGATTGA GGCTGCCAAG AGGAACGACT TCTGTAAGCT CCAGGAGCTG CACCGAGCTG 
     3481 GGGGCGACCT CATGCACCGA GACGAGCAGA GTCGCACGCT CCTGCACCAC GCAGTCAGCA 
     3541 CTGGCAGCAA GGATGTGGTC CGCTACCTGC TGGACCACGC CCCCCCAGAG ATCCTTGATG 
     3601 CGGTGGAGGA AAACGGGGAG ACCTGTTTGC ACCAAGCAGC GGCCCTGGGC CAGCGCACCA 
     3661 TCTGCCACTA CATCGTGGAG GCCGGGGCCT CGCTCATGAA GACAGACCAG CAGGGCGACA 
     3721 CTCCCCGGCA GCGGGCTGAG AAGGCTCAGG ACACCGAGCT GGCCGCCTAC CTGGAGAACC 
     3781 GGCAGCACTA CCAGATGATC CAGCGGGAGG ACCAGGAGAC GGCTGTGACG CGTACGCGGC 
     3841 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3901 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3961 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4021 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4081 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4141 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4201 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4261 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4321 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4381 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4441 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4501 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4561 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4621 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4681 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5281 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5341 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5401 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5461 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5521 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5581 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5641 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5701 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5761 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5821 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5881 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5941 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6001 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6061 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6121 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6181 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6241 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6301 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6361 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6421 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6481 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6541 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6601 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6661 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6721 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6781 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6841 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6901 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7021 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7081 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7141 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7201 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7261 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7321 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7381 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7441 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7501 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7561 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7621 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//