LOCUS       RC216209                7314 bp    DNA     circular  SYN 
DEFINITION  TLR10 (Myc-DDK-tagged)-Human toll-like receptor 10 (TLR10), transcript variant 2
ACCESSION   NM_001017388
VERSION     NM_001017388.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3471..3562 
                     /label="Myc-DDK"  
     misc_feature    3592..4177 
                     /label="PolyA signal"  
     misc_feature    3649..3668 
                     /label="Restriction Enzyme"  
     misc_feature    4453..5125 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Kan/Neo"  
     misc_feature    6384..6686 
                     /label="SV40 ori"  
     misc_feature    6772..7249 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3453..3459 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3453 
                     /label="TLR10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTCATC AGAAACATTT ACATATTTTG TAGTATTGTT ATGACAGCAG 
     1081 AGGGTGATGC TCCAGAGCTG CCAGAAGAAA GGGAACTGAT GACCAACTGC TCCAACATGT 
     1141 CTCTAAGAAA GGTTCCCGCA GACTTGACCC CAGCCACAAC GACACTGGAT TTATCCTATA 
     1201 ACCTCCTTTT TCAACTCCAG AGTTCAGATT TTCATTCTGT CTCCAAACTG AGAGTTTTGA 
     1261 TTCTATGCCA TAACAGAATT CAACAGCTGG ATCTCAAAAC CTTTGAATTC AACAAGGAGT 
     1321 TAAGATATTT AGATTTGTCT AATAACAGAC TGAAGAGTGT AACTTGGTAT TTACTGGCAG 
     1381 GTCTCAGGTA TTTAGATCTT TCTTTTAATG ACTTTGACAC CATGCCTATC TGTGAGGAAG 
     1441 CTGGCAACAT GTCACACCTG GAAATCCTAG GTTTGAGTGG GGCAAAAATA CAAAAATCAG 
     1501 ATTTCCAGAA AATTGCTCAT CTGCATCTAA ATACTGTCTT CTTAGGATTC AGAACTCTTC 
     1561 CTCATTATGA AGAAGGTAGC CTGCCCATCT TAAACACAAC AAAACTGCAC ATTGTTTTAC 
     1621 CAATGGACAC AAATTTCTGG GTTCTTTTGC GTGATGGAAT CAAGACTTCA AAAATATTAG 
     1681 AAATGACAAA TATAGATGGC AAAAGCCAAT TTGTAAGTTA TGAAATGCAA CGAAATCTTA 
     1741 GTTTAGAAAA TGCTAAGACA TCGGTTCTAT TGCTTAATAA AGTTGATTTA CTCTGGGACG 
     1801 ACCTTTTCCT TATCTTACAA TTTGTTTGGC ATACATCAGT GGAACACTTT CAGATCCGAA 
     1861 ATGTGACTTT TGGTGGTAAG GCTTATCTTG ACCACAATTC ATTTGACTAC TCAAATACTG 
     1921 TAATGAGAAC TATAAAATTG GAGCATGTAC ATTTCAGAGT GTTTTACATT CAACAGGATA 
     1981 AAATCTATTT GCTTTTGACC AAAATGGACA TAGAAAACCT GACAATATCA AATGCACAAA 
     2041 TGCCACACAT GCTTTTCCCG AATTATCCTA CGAAATTCCA ATATTTAAAT TTTGCCAATA 
     2101 ATATCTTAAC AGACGAGTTG TTTAAAAGAA CTATCCAACT GCCTCACTTG AAAACTCTCA 
     2161 TTTTGAATGG CAATAAACTG GAGACACTTT CTTTAGTAAG TTGCTTTGCT AACAACACAC 
     2221 CCTTGGAACA CTTGGATCTG AGTCAAAATC TATTACAACA TAAAAATGAT GAAAATTGCT 
     2281 CATGGCCAGA AACTGTGGTC AATATGAATC TGTCATACAA TAAATTGTCT GATTCTGTCT 
     2341 TCAGGTGCTT GCCCAAAAGT ATTCAAATAC TTGACCTAAA TAATAACCAA ATCCAAACTG 
     2401 TACCTAAAGA GACTATTCAT CTGATGGCCT TACGAGAACT AAATATTGCA TTTAATTTTC 
     2461 TAACTGATCT CCCTGGATGC AGTCATTTCA GTAGACTTTC AGTTCTGAAC ATTGAAATGA 
     2521 ACTTCATTCT CAGCCCATCT CTGGATTTTG TTCAGAGCTG CCAGGAAGTT AAAACTCTAA 
     2581 ATGCGGGAAG AAATCCATTC CGGTGTACCT GTGAATTAAA AAATTTCATT CAGCTTGAAA 
     2641 CATATTCAGA GGTCATGATG GTTGGATGGT CAGATTCATA CACCTGTGAA TACCCTTTAA 
     2701 ACCTAAGGGG AACTAGGTTA AAAGACGTTC ATCTCCACGA ATTATCTTGC AACACAGCTC 
     2761 TGTTGATTGT CACCATTGTG GTTATTATGC TAGTTCTGGG GTTGGCTGTG GCCTTCTGCT 
     2821 GTCTCCACTT TGATCTGCCC TGGTATCTCA GGATGCTAGG TCAATGCACA CAAACATGGC 
     2881 ACAGGGTTAG GAAAACAACC CAAGAACAAC TCAAGAGAAA TGTCCGATTC CACGCATTTA 
     2941 TTTCATACAG TGAACATGAT TCTCTGTGGG TGAAGAATGA ATTGATCCCC AATCTAGAGA 
     3001 AGGAAGATGG TTCTATCTTG ATTTGCCTTT ATGAAAGCTA CTTTGACCCT GGCAAAAGCA 
     3061 TTAGTGAAAA TATTGTAAGC TTCATTGAGA AAAGCTATAA GTCCATCTTT GTTTTGTCTC 
     3121 CCAACTTTGT CCAGAATGAG TGGTGCCATT ATGAATTCTA CTTTGCCCAC CACAATCTCT 
     3181 TCCATGAAAA TTCTGATCAT ATAATTCTTA TCTTACTGGA ACCCATTCCA TTCTATTGCA 
     3241 TTCCCACCAG GTATCATAAA CTGAAAGCTC TCCTGGAAAA AAAAGCATAC TTGGAATGGC 
     3301 CCAAGGATAG GCGTAAATGT GGGCTTTTCT GGGCAAACCT TCGAGCTGCT ATTAATGTTA 
     3361 ATGTATTAGC CACCAGAGAA ATGTATGAAC TGCAGACATT CACAGAGTTA AATGAAGAGT 
     3421 CTCGAGGTTC TACAATCTCT CTGATGAGAA CAGATTGTCT AACGCGTACG CGGCCGCTCG 
     3481 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3541 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3601 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3661 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3721 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3781 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3841 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3901 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3961 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4021 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4081 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4141 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4201 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4261 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4321 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4981 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5041 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5101 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5161 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5221 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5281 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5341 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5401 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5461 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5521 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5581 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5641 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5701 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5761 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5821 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5881 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5941 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6001 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6061 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6121 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6181 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6241 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6301 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6361 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6421 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6481 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6541 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6661 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6721 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6781 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6841 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6901 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6961 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7021 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7081 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7141 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7201 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7261 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//