LOCUS       RC216195                5457 bp    DNA     circular  SYN 
DEFINITION  LCN12 (Myc-DDK-tagged)-Human lipocalin 12 (LCN12)
ACCESSION   NM_178536
VERSION     NM_178536.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    1614..1705 
                     /label="Myc-DDK"  
     misc_feature    1735..2320 
                     /label="PolyA signal"  
     misc_feature    1792..1811 
                     /label="Restriction Enzyme"  
     misc_feature    2596..3268 
                     /label="ColE1"  
     misc_feature    3698..4492 
                     /label="Kan/Neo"  
     misc_feature    4527..4829 
                     /label="SV40 ori"  
     misc_feature    4915..5392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    1596..1602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..1596 
                     /label="LCN12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTGCTG TGTGGCCTGT GGCTGTGGCT CTCCTTGCTG AAAGTCCTGC 
     1081 AGGCCCAGAC CCCAACCCCC CTGCCACTCC CGCCCCCGAT GCAGAGCTTC CAAGGAAACC 
     1141 AGTTCCAGGG GGAATGGTTC GTCCTGGGCC TGGCGGGCAA CAGCTTCAGG CCGGAGCACA 
     1201 GGGCGCTGCT GAACGCTTTC ACCGCAACTT TTGAGCTAAG TGATGATGGC CGCTTTGAGG 
     1261 TGTGGAATGC GATGACTCGA GGCCAGCACT GTGACACATG GTCTTATGTG CTGATACCGG 
     1321 CAGCCCAGCC TGGGCAGTTC ACTGTGGACC ACGGTGTGGA GCCCGGGGCG GACAGAGAGG 
     1381 AGACCCGGGT GGTGGACAGC GACTACACCC AGTTCGCCCT GATGCTGTCC CGCAGACACA 
     1441 CGAGCAGGCT GGCCGTCCTC AGGATCAGCC TGCTGGGCAG GAGCTGGTTG CTGCCTCCCG 
     1501 GGACGCTGGA CCAGTTCATC TGCCTGGGCA GAGCTCAGGG CCTCTCGGAT GACAACATCG 
     1561 TCTTCCCAGA TGTGACTGGC TGGTCACCCC AGGCCAGCGT CTGTACGCGT ACGCGGCCGC 
     1621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     1681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     1741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     1801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     1861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     1921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     1981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     2041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     2101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     2161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     2221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     2281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     2341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     2401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     2461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     2521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     2581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     2641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     2701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     2761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     2821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     2881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     2941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     3001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     3061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     3121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     3181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     3241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     3301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     3361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     3421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     3481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     3541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     3601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     3661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     3721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     3781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     3841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     3901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     3961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     4021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     4081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     4141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     4201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     4261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     4321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     4381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     4441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     4501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     4561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     4621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     4681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     4741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     4801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     4861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     4921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     4981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     5041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     5101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     5161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     5221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     5281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     5341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     5401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//