LOCUS       RC216154                8592 bp    DNA     circular  SYN 
DEFINITION  COL6A3 (Myc-DDK-tagged)-Human collagen, type VI, alpha 3 (COL6A3), transcript variant 3
ACCESSION   NM_057165
VERSION     NM_057165.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4749..4840 
                     /label="Myc-DDK"  
     misc_feature    4870..5455 
                     /label="PolyA signal"  
     misc_feature    4927..4946 
                     /label="Restriction Enzyme"  
     misc_feature    5731..6403 
                     /label="ColE1"  
     misc_feature    6833..7627 
                     /label="Kan/Neo"  
     misc_feature    7662..7964 
                     /label="SV40 ori"  
     misc_feature    8050..8527 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4731..4737 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4731 
                     /label="COL6A3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAAACAT CGGCACTTGC CCTTAGTGGC CGTCTTTTGC CTCTTTCTCT 
     1081 CAGGCTTTCC TACAACTCAT GCCCAGCAGC AGCAAGCAGC ACAAGACTCT GCTGACATTA 
     1141 TTTTCCTTAT TGATGGATCA AACAACACCG GAAGTGTCAA TTTCGCAGTC ATTCTCGACT 
     1201 TCCTTGTAAA TCTCCTTGAG AAACTCCCAA TTGGAACTCA GCAGATCCGA GTGGGGGTGG 
     1261 TCCAGTTTAG CGATGAGCCC AGAACCATGT TCTCCTTGGA CACCTACTCC ACCAAGGCCC 
     1321 AGGTTCTGGG TGCAGTGAAA GCCCTCGGGT TTGCTGGTGG GGAGTTGGCC AATATCGGCC 
     1381 TCGCCCTTGA TTTCGTGGTG GAGAACCACT TCACCCGGGC AGGGGGCAGC CGCGTGGAGG 
     1441 AAGGGGTTCC CCAGGTGCTG GTCCTCATAA GTGCCGGGCC TTCTAGTGAC GAGATTCGCT 
     1501 ACGGGGTGGT AGCACTGAAG CAGGCTAGCG TGTTCTCATT CGGCCTTGGA GCCCAGGCCG 
     1561 CCTCCAGGGC AGAGCTTCAG CACATAGCTA CCGATGACAA CTTGGTGTTT ACTGTCCCGG 
     1621 AATTCCGTAG CTTTGGGGAC CTCCAGGAGA AATTACTGCC GTACATTGTT GGCGTGGCCC 
     1681 AAAGGCACAT TGTCTTGAAA CCGCCAACCA TTGTCACACA AGTCATTGAA GTCAACAAGA 
     1741 GAGACATAGT CTTCCTGGTG GATGGCTCAT CTGCACTGGG ACTGGCCAAC TTCAATGCCA 
     1801 TCCGAGACTT CATTGCTAAA GTCATCCAGA GGCTGGAAAT CGGACAGGAT CTTATCCAGG 
     1861 TGGCAGTGGC CCAGTATGCA GACACTGTGA GGCCTGAATT TTATTTCAAT ACCCATCCAA 
     1921 CAAAAAGGGA AGTCATAACC GCTGTGCGGA AAATGAAGCC CCTGGACGGC TCGGCCCTGT 
     1981 ACACGGGCTC TGCTCTAGAC TTTGTTCGTA ACAACCTATT CACGAGTTCA GCCGGCTACC 
     2041 GGGCTGCCGA GGGGATTCCT AAGCTTTTGG TGCTGATCAC AGGTGGTAAG TCCCTAGATG 
     2101 AAATCAGCCA GCCTGCCCAG GAGCTGAAGA GAAGCAGCAT AATGGCCTTT GCCATTGGGA 
     2161 ACAAGGGTGC CGATCAGGCT GAGCTGGAAG AGATCGCTTT CGACTCCTCC CTGGTGTTCA 
     2221 TCCCAGCTGA GTTCCGAGCC GCCCCATTGC AAGGCATGCT GCCTGGCTTG CTGGCACCTC 
     2281 TCAGGACCCT CTCTGGAACC CCTGAAGTTC ACTCAAACAA AAGGGATATC ATCTTTCTTT 
     2341 TGGATGGATC AGCCAACGTT GGAAAAACCA ATTTCCCTTA TGTGCGCGAC TTTGTAATGA 
     2401 ACCTAGTTAA CAGCCTTGAT ATTGGAAATG ACAATATTCG TGTTGGTTTA GTGCAATTTA 
     2461 GTGACACTCC TGTAACGGAG TTCTCTTTAA ACACATACCA GACCAAGTCA GATATCCTTG 
     2521 GTCATCTGAG GCAGCTGCAG CTCCAGGGAG GTTCGGGCCT GAACACAGGC TCAGCCCTAA 
     2581 GCTATGTCTA TGCCAACCAC TTCACGGAAG CTGGCGGCAG CAGGATCCGT GAACACGTGC 
     2641 CGCAGCTCCT GCTTCTGCTC ACAGCTGGGC AGTCTGAGGA CTCCTATTTG CAAGCTGCCA 
     2701 ACGCCTTGAC ACGCGCGGGC ATCCTGACTT TTTGTGTGGG AGCTAGCCAG GCGAATAAGG 
     2761 CAGAGCTTGA GCAGATTGCT TTTAACCCAA GCCTGGTGTA TCTCATGGAT GATTTCAGCT 
     2821 CCCTGCCAGC TTTGCCTCAG CAGCTGATTC AGCCCCTAAC CACATATGTT AGTGGAGGTG 
     2881 TGGAGGAAGT ACCACTCGCT CAGCCAGAGA GCAAGCGAGA CATTCTGTTC CTCTTTGACG 
     2941 GCTCAGCCAA TCTTGTGGGC CAGTTCCCTG TTGTCCGTGA CTTTCTCTAC AAGATTATCG 
     3001 ATGAGCTCAA TGTGAAGCCA GAGGGGACCC GAATTGCGGT GGCTCAGTAC AGCGATGATG 
     3061 TCAAGGTGGA GTCCCGTTTT GATGAGCACC AGAGTAAGCC TGAGATCCTG AATCTTGTGA 
     3121 AGAGAATGAA GATCAAGACG GGCAAAGCCC TCAACCTGGG CTACGCGCTG GACTATGCAC 
     3181 AGAGGTACAT TTTTGTGAAG TCTGCTGGCA GCCGGATCGA GGATGGAGTG CTTCAGTTCC 
     3241 TGGTGCTGCT GGTCGCAGGA AGGTCATCTG ACCGTGTGGA TGGGCCAGCA AGTAACCTGA 
     3301 AGCAGAGTGG GGTTGTGCCT TTCATCTTCC AAGCCAAGAA CGCAGACCCT GCTGAGTTAG 
     3361 AGCAGATCGT GCTGTCTCCA GCGTTTATCC TGGCTGCAGA GTCGCTTCCC AAGATTGGAG 
     3421 ATCTTCATCC ACAGATAGTG AATCTCTTAA AATCAGTGCA CAACGGAGCA CCAGCACCAG 
     3481 TTTCAGGTGA AAAGGACGTG GTGTTTCTGC TTGATGGCTC TGAGGGCGTC AGGAGCGGCT 
     3541 TCCCTCTGTT GAAAGAGTTT GTCCAGAGAG TGGTGGAAAG CCTGGATGTG GGCCAGGACC 
     3601 GGGTCCGCGT GGCCGTGGTG CAGTACAGCG ACCGGACCAG GCCCGAGTTC TACCTGAATT 
     3661 CATACATGAA CAAGCAGGAC GTCGTCAACG CTGTCCGCCA GCTGACCCTG CTGGGAGGGC 
     3721 CGACCCCCAA CACCGGGGCC GCCCTGGAGT TTGTCCTGAG GAACATCCTG GTCAGCTCTG 
     3781 CGGGAAGCAG GATAACAGAA GGTGTGCCCC AGCTGCTGAT CGTCCTCACG GCCGACAGGT 
     3841 CTGGGGATGA TGTGCGGAAC CCCTCCGTGG TCGTGAAGAG GGGTGGGGCT GTGCCCATTG 
     3901 GCATTGGCAT CGGGAACGCT GACATCACAG AGATGCAGAC CATCTCCTTC ATCCCGGACT 
     3961 TTGCCGTGGC CATTCCCACC TTTCGCCAGC TGGGGACCGT CCAACAGGTC ATCTCTGAGA 
     4021 GGGTGACCCA GCTCACCCGC GAGGAGCTGA GCAGGCTGCA GCCGGTGTTG CAGCCTCTAC 
     4081 CGAGCCCAGG TGTTGGTGGC AAGAGGGACG TGGTCTTTCT CATCGATGGG TCCCAAAGTG 
     4141 CCGGGCCTGA GTTCCAGTAC GTTCGCACCC TCATAGAGAG GCTGGTTGAC TACCTGGACG 
     4201 TGGGCTTTGA CACCACCCGG GTGGCTGTCA TCCAGTTCAG CGATGACCCC AAGGTGGAGT 
     4261 TCCTGCTGAA CGCCCATTCC AGCAAGGATG AAGTGCAGAA CGCGGTGCAG CGGCTGAGGC 
     4321 CCAAGGGAGG GCGGCAGATC AACGTGGGCA ATGCCCTGGA GTACGTGTCC AGGAACATCT 
     4381 TCAAGAGGCC CCTGGGGAGC CGCATTGAAG AGGGCGTCCC GCAGTTCCTG GTCCTCATCT 
     4441 CGTCTGGAAA GTCTGACGAT GAGGTGGACG ACCCGGCGGT GGAGCTCAAG CAGTTTGGCG 
     4501 TGGCCCCTTT CACGATCGCC AGGAACGCAG ACCAGGAGGA GCTGGTGAAG ATCTCGCTGA 
     4561 GCCCCGAATA TGTGTTCTCG GTGAGCACCT TCCGGGAGCT GCCCAGCCTG GAGCAGAAAC 
     4621 TGCTGACGCC CATCACGACC CTGACCTCAG AGCAGATCCA GAAGCTCTTA GCCAGCACTC 
     4681 GCTATCCACC TCCAGGTGAG ATGGGGGCGT CGGAGGTTCT CCTTGGAGCA TTTTCCATAA 
     4741 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4801 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4861 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4921 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4981 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5041 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5101 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5161 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5221 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5281 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5341 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5401 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5461 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5521 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5581 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5641 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5701 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5761 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5821 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5881 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5941 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6001 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6061 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6121 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6181 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6241 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6301 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6361 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6421 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6481 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6541 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6601 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6661 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6721 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6781 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6841 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6901 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6961 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7021 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7081 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7141 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7201 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7261 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7321 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7381 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7441 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7501 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7561 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7621 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7681 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7741 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7801 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7861 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7921 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7981 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8041 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8101 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8161 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8221 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8281 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8341 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8401 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8461 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8521 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8581 AACGCGAATT TT
//