LOCUS       RC216108                8511 bp    DNA     circular  SYN 
DEFINITION  HIPK1 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 1 (HIPK1), transcript variant 1
ACCESSION   NM_198268
VERSION     NM_198268.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="HIPK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCACAG CTGCAAGTGT TTTCGCCCCC ATCAGTGTCG TCGAGTGCCT 
     1081 TCTGCAGTGC GAAGAAACTG AAAATAGAGC CCTCTGGCTG GGATGTTTCA GGACAGAGTA 
     1141 GCAACGACAA ATATTATACC CACAGCAAAA CCCTCCCAGC CACACAAGGG CAAGCCAACT 
     1201 CCTCTCACCA GGTAGCAAAT TTCAACATCC CTGCTTACGA CCAGGGCCTC CTCCTCCCAG 
     1261 CTCCTGCAGT GGAGCATATT GTTGTAACAG CCGCTGATAG CTCGGGCAGT GCTGCTACAT 
     1321 CAACCTTCCA AAGCAGCCAG ACCCTGACTC ACAGAAGCAA CGTTTCTTTG CTTGAGCCAT 
     1381 ATCAAAAATG TGGATTGAAA CGAAAAAGTG AGGAAGTTGA CAGCAACGGT AGTGTGCAGA 
     1441 TCATAGAAGA ACATCCCCCT CTCATGCTGC AAAACAGGAC TGTGGTGGGT GCTGCTGCCA 
     1501 CAACCACCAC TGTGACCACA AAGAGTAGCA GTTCCAGCGG AGAAGGGGAT TACCAGCTGG 
     1561 TCCAGCATGA GATCCTTTGC TCTATGACCA ATAGCTATGA AGTCTTGGAG TTCCTAGGCC 
     1621 GGGGGACATT TGGACAGGTG GCTAAGTGCT GGAAGAGGAG CACCAAGGAA ATTGTGGCTA 
     1681 TTAAAATCTT GAAGAACCAC CCCTCCTATG CCAGACAAGG ACAGATTGAA GTGAGCATCC 
     1741 TTTCCCGCCT AAGCAGTGAA AATGCTGATG AGTATAATTT TGTCCGTTCA TACGAGTGCT 
     1801 TTCAGCATAA GAATCACACC TGCCTTGTTT TTGAAATGTT GGAGCAGAAC TTATATGATT 
     1861 TTCTAAAGCA AAACAAATTT AGCCCACTGC CACTCAAGTA CATCAGACCA ATCTTGCAGC 
     1921 AGGTGGCCAC AGCCTTGATG AAGCTCAAGA GTCTTGGTCT GATCCACGCT GACCTTAAGC 
     1981 CTGAAAACAT CATGCTGGTT GATCCAGTTC GCCAGCCCTA CCGAGTGAAG GTCATTGACT 
     2041 TTGGTTCTGC TAGTCACGTT TCCAAAGCTG TGTGCTCAAC CTACTTACAG TCACGTTACT 
     2101 ACAGAGCTCC TGAAATTATT CTTGGGTTAC CATTTTGTGA AGCTATTGAT ATGTGGTCAC 
     2161 TGGGCTGTGT GATAGCTGAG CTGTTCCTGG GATGGCCTCT TTATCCTGGT GCTTCAGAAT 
     2221 ATGATCAGAT TCGTTATATT TCACAAACAC AAGGCTTGCC AGCTGAATAT CTTCTCAGTG 
     2281 CCGGAACAAA AACAACCAGG TTTTTCAACA GAGATCCTAA TTTGGGGTAC CCACTGTGGA 
     2341 GGCTTAAGAC ACCTGAAGAA CATGAACTGG AGACTGGAAT AAAATCAAAA GAAGCTCGGA 
     2401 AGTACATTTT TAATTGCTTA GATGACATGG CTCAGGTGAA TATGTCTACA GACCTGGAGG 
     2461 GAACAGACAT GTTGGCAGAG AAGGCAGACC GAAGAGAATA CATTGATCTG TTAAAGAAAA 
     2521 TGCTCACAAT TGATGCAGAT AAGAGAATTA CCCCTCTAAA AACTCTTAAC CATCAGTTTG 
     2581 TGACAATGAC TCACCTTTTG GATTTTCCAC ATAGCAATCA TGTTAAGTCT TGTTTTCAGA 
     2641 ACATGGAGAT CTGCAAGCGG AGGGTTCACA TGTATGATAC AGTGAGTCAG ATCAAGAGTC 
     2701 CCTTCACTAC ACATGTTGCC CCAAATACAA GCACAAATCT AACCATGAGC TTCAGCAATC 
     2761 AGCTCAATAC AGTGCACAAT CAGGCCAGTG TTCTAGCTTC CAGTTCTACT GCAGCAGCTG 
     2821 CTACTCTTTC TCTGGCTAAT TCAGATGTCT CACTACTAAA CTACCAGTCA GCTTTGTACC 
     2881 CATCATCTGC TGCACCAGTT CCTGGAGTTG CCCAGCAGGG TGTTTCCTTG CAGCCTGGAA 
     2941 CCACCCAGAT TTGCACTCAG ACAGATCCAT TCCAACAGAC ATTTATAGTA TGTCCACCTG 
     3001 CGTTTCAAAC TGGACTACAA GCAACAACAA AGCATTCTGG ATTCCCTGTG AGGATGGATA 
     3061 ATGCTGTACC GATTGTACCC CAGGCACCAG CTGCTCAGCC ACTACAGATT CAGTCAGGAG 
     3121 TTCTCACGCA GGGAAGCTGT ACACCACTAA TGGTAGCAAC TCTCCACCCT CAAGTAGCCA 
     3181 CCATCACACC GCAGTATGCG GTGCCCTTTA CTCTGAGCTG CGCAGCCGGC CGGCCGGCGC 
     3241 TGGTTGAACA GACTGCCGCT GTACTGCAGG CGTGGCCTGG AGGGACTCAG CAAATTCTCC 
     3301 TGCCTTCAAC TTGGCAACAG TTGCCTGGGG TAGCTCTACA CAACTCTGTC CAGCCCACAG 
     3361 CAATGATTCC AGAGGCCATG GGGAGTGGAC AGCAGCTAGC TGACTGGAGG AATGCCCACT 
     3421 CTCATGGCAA CCAGTACAGC ACTATCATGC AGCAGCCATC CTTGCTGACT AACCATGTGA 
     3481 CATTGGCCAC TGCTCAGCCT CTGAATGTTG GTGTTGCCCA TGTTGTCAGA CAACAACAAT 
     3541 CCAGTTCCCT CCCTTCGAAG AAGAATAAGC AGTCAGCTCC AGTCTCTTCC AAGTCCTCTC 
     3601 TAGATGTTCT GCCTTCCCAA GTCTATTCTC TGGTTGGGAG CAGTCCCCTC CGCACCACAT 
     3661 CTTCTTATAA TTCCTTGGTC CCTGTCCAAG ATCAGCATCA GCCCATCATC ATTCCAGATA 
     3721 CTCCCAGCCC TCCTGTGAGT GTCATCACTA TCCGAAGTGA CACTGATGAG GAAGAGGACA 
     3781 ACAAATACAA GCCCAGTAGC TCTGGACTGA AGCCAAGGTC TAATGTCATC AGTTATGTCA 
     3841 CTGTCAATGA TTCTCCAGAC TCTGACTCTT CTTTGAGCAG CCCTTATTCC ACTGATACCC 
     3901 TGAGTGCTCT CCGAGGCAAT AGTGGATCCG TTTTGGAGGG GCCTGGCAGA GTTGTGGCAG 
     3961 ATGGCACTGG CACCCGCACT ATCATTGTGC CTCCACTGAA AACTCAGCTT GGTGACTGCA 
     4021 CTGTAGCAAC CCAGGCCTCA GGTCTCCTGA GCAATAAGAC TAAGCCAGTC GCTTCAGTGA 
     4081 GTGGGCAGTC ATCTGGATGC TGTATCACCC CCACAGGGTA TCGAGCTCAA CGCGGGGGGA 
     4141 CCAGTGCAGC ACAACCACTC AATCTTAGCC AGAACCAGCA GTCATCGGCG GCTCCAACCT 
     4201 CACAGGAGAG AAGCAGCAAC CCAGCCCCCC GCAGGCAGCA GGCGTTTGTG GCCCCTCTCT 
     4261 CCCAAGCCCC CTACACCTTC CAGCATGGCA GCCCGCTACA CTCGACAGGG CACCCACACC 
     4321 TTGCCCCGGC CCCTGCTCAC CTGCCAAGCC AGGCTCATCT GTATACGTAT GCTGCCCCGA 
     4381 CTTCTGCTGC TGCACTGGGC TCAACCAGCT CCATTGCTCA TCTTTTCTCC CCACAGGGTT 
     4441 CCTCAAGGCA TGCTGCAGCC TATACCACTC ACCCTAGCAC TTTGGTGCAC CAGGTCCCTG 
     4501 TCAGTGTTGG GCCCAGCCTC CTCACTTCTG CCAGCGTGGC CCCTGCTCAG TACCAACACC 
     4561 AGTTTGCCAC CCAATCCTAC ATTGGGTCTT CCCGAGGCTC AACAATTTAC ACTGGATACC 
     4621 CGCTGAGTCC TACCAAGATC AGCCAGTATT CCTACTTAAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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