LOCUS       RC216099L3                9377 bp    DNA     circular  SYN 
DEFINITION  HCN3 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)
ACCESSION   NM_020897
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7668..7848 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8576..9235 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7896..8515 
                     /label="ColE1"  
     misc_feature    5708..5731 
                     /label="DDK"  
     misc_feature    6543..6563 
                     /label="LR50"  
     misc_feature    5660..5731 
                     /label="MYC"  
     misc_feature    5741..5806 
                     /label="P2A"  
     misc_feature    5807..6406 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6552..7143 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5642..5648 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5642 
                     /label="HCN3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGGCAGAG CAGCGGCCGG CGGCGGGGGC 
     3361 CAGCGAAGGG GCGACCCCTG GACTGGAGGC GGTGCCTCCC GTTGCTCCCC CGCCTGCGAC 
     3421 CGCGGCCTCA GGTCCGATCC CCAAATCTGG GCCTGAGCCT AAGAGGAGGC ACCTTGGGAC 
     3481 GCTGCTCCAG CCTACGGTCA ACAAGTTCTC CCTTCGGGTG TTCGGCAGCC ACAAAGCAGT 
     3541 GGAAATCGAG CAGGAGCGGG TGAAGTCAGC GGGGGCCTGG ATCATCCACC CCTACAGCGA 
     3601 CTTCCGGTTT TACTGGGACC TGATCATGCT GCTGCTGATG GTGGGGAACC TCATCGTCCT 
     3661 GCCTGTGGGC ATCACCTTCT TCAAGGAGGA GAACTCCCCG CCTTGGATCG TCTTCAACGT 
     3721 ATTGTCTGAT ACTTTCTTCC TACTGGATCT GGTGCTCAAC TTCCGAACGG GCATCGTGGT 
     3781 GGAGGAGGGT GCTGAGATCC TGCTGGCACC GCGGGCCATC CGCACGCGCT ACCTGCGCAC 
     3841 CTGGTTCCTG GTTGACCTCA TCTCTTCTAT CCCTGTGGAT TACATCTTCC TAGTGGTGGA 
     3901 GCTGGAGCCA CGGTTGGACG CTGAGGTCTA CAAAACGGCA CGGGCCCTAC GCATCGTTCG 
     3961 CTTCACCAAG ATCCTAAGCC TGCTGAGGCT GCTCCGCCTC TCCCGCCTCA TCCGCTACAT 
     4021 ACACCAGTGG GAGGAGATCT TTCACATGAC CTATGACCTG GCCAGTGCTG TGGTTCGCAT 
     4081 CTTCAACCTC ATTGGGATGA TGCTGCTGCT ATGTCACTGG GATGGCTGTC TGCAGTTCCT 
     4141 GGTGCCCATG CTGCAGGACT TCCCTCCCGA CTGCTGGGTC TCCATCAACC ACATGGTGAA 
     4201 CCACTCGTGG GGCCGCCAGT ATTCCCATGC CCTGTTCAAG GCCATGAGCC ACATGCTGTG 
     4261 CATTGGCTAT GGGCAGCAGG CACCTGTAGG CATGCCCGAC GTCTGGCTCA CCATGCTCAG 
     4321 CATGATCGTA GGTGCCACAT GCTACGCCAT GTTCATCGGC CATGCCACGG CACTCATCCA 
     4381 GTCCCTGGAC TCTTCCCGGC GTCAGTACCA GGAGAAGTAC AAGCAGGTGG AGCAGTACAT 
     4441 GTCCTTCCAC AAGCTGCCAG CAGACACGCG GCAGCGCATC CACGAGTACT ATGAGCACCG 
     4501 CTACCAGGGC AAGATGTTCG ATGAGGAAAG CATCCTGGGC GAGCTGAGCG AGCCGCTTCG 
     4561 CGAGGAGATC ATTAACTTCA CCTGTCGGGG CCTGGTGGCC CACATGCCGC TGTTTGCCCA 
     4621 TGCCGACCCC AGCTTCGTCA CTGCAGTTCT CACCAAGCTG CGCTTTGAGG TCTTCCAGCC 
     4681 GGGGGATCTC GTGGTGCGTG AGGGCTCCGT GGGGAGGAAG ATGTACTTCA TCCAGCATGG 
     4741 GCTGCTCAGT GTGCTGGCCC GCGGCGCCCG GGACACACGC CTCACCGATG GATCCTACTT 
     4801 TGGGGAGATC TGCCTGCTAA CTAGGGGCCG GCGCACAGCC AGTGTTCGGG CTGACACCTA 
     4861 CTGCCGCCTT TACTCACTCA GCGTGGACCA TTTCAATGCT GTGCTTGAGG AGTTCCCCAT 
     4921 GATGCGCCGG GCCTTTGAGA CTGTGGCCAT GGATCGGCTG CTCCGCATCG GCAAGAAGAA 
     4981 TTCCATACTG CAGCGGAAGC GCTCCGAGCC AAGTCCAGGC AGCAGTGGTG GCATCATGGA 
     5041 GCAGCACTTG GTGCAACATG ACAGAGACAT GGCTCGGGGT GTTCGGGGTC GGGCCCCGAG 
     5101 CACAGGAGCT CAGCTTAGTG GAAAGCCAGT ACTGTGGGAG CCACTGGTAC ATGCGCCCCT 
     5161 TCAGGCAGCT GCTGTGACCT CCAATGTGGC CATTGCCCTG ACTCATCAGC GGGGCCCTCT 
     5221 GCCCCTCTCC CCTGACTCTC CAGCCACCCT CCTTGCTCGC TCTGCTTGGC GCTCAGCAGG 
     5281 CTCTCCAGCT TCCCCGCTGG TGCCCGTCCG AGCTGGCCCA TGGGCATCCA CCTCCCGCCT 
     5341 GCCCGCCCCA CCTGCCCGAA CCCTGCACGC CAGCCTATCC CGGGCAGGGC GCTCCCAGGT 
     5401 CTCCCTGCTG GGTCCCCCTC CAGGAGGAGG TGGACGGCGG CTAGGACCTC GGGGCCGCCC 
     5461 ACTCTCAGCC TCCCAACCCT CTCTGCCTCA GCGGGCAACA GGCGATGGCT CTCCTGGGCG 
     5521 TAAGGGATCA GGAAGTGAGC GGCTGCCTCC CTCAGGGCTC CTGGCCAAAC CTCCAAGGAC 
     5581 AGCCCAGCCC CCCAGGCCAC CAGTGCCTGA GCCAGCCACA CCCCGGGGTC TCCAGCTTTC 
     5641 TGCCAACATG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     5701 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTGGGTAG GAAGCGGAGC 
     5761 TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTATGAC 
     5821 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC 
     5881 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGATCGCCA 
     5941 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG 
     6001 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT 
     6061 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG 
     6121 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC 
     6181 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC 
     6241 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC 
     6301 CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT 
     6361 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGATGTAC 
     6421 ACCCAAACGG CCGGCCGCGG TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG 
     6481 TATAGCATAC ATTATACGAA GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT 
     6541 TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 
     6601 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 
     6661 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 
     6721 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 
     6781 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 
     6841 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 
     6901 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT 
     6961 CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 
     7021 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 
     7081 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 
     7141 CCTCCCCGCA TCGATACCGT CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA 
     7201 AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG 
     7261 GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT 
     7321 GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA 
     7381 CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG 
     7441 CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG 
     7501 CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG 
     7561 TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG 
     7621 TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG 
     7681 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 
     7741 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 
     7801 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC 
     7861 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 
     7921 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 
     7981 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 
     8041 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 
     8101 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 
     8161 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 
     8221 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 
     8281 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 
     8341 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 
     8401 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 
     8461 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 
     8521 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 
     8581 ATGATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC 
     8641 CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT 
     8701 TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT 
     8761 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA 
     8821 TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT 
     8881 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA 
     8941 ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA 
     9001 GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA 
     9061 TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG 
     9121 TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA 
     9181 AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT 
     9241 CCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     9301 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     9361 GAAAAGTGCC ACCTGAC
//