LOCUS       RC216096                8457 bp    DNA     circular  SYN 
DEFINITION  ATP8B4 (Myc-DDK-tagged)-Human ATPase, class I, type 8B, member 4 (ATP8B4)
ACCESSION   NM_024837
VERSION     NM_024837.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="ATP8B4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCTGCAGT GAAAAGAAAT TGCGTGAAGT GGAACGGATA GTGAAAGCCA 
     1081 ATGACCGTGA ATATAATGAA AAGTTCCAGT ATGCGGATAA TCGTATCCAC ACATCGAAAT 
     1141 ATAATATTCT CACCTTCTTG CCAATTAATT TATTTGAACA GTTCCAAAGA GTGGCAAATG 
     1201 CCTATTTTCT TTGCCTTCTG ATTTTACAGC TAATTCCAGA AATTTCCTCC TTGACCTGGT 
     1261 TTACCACCAT TGTGCCTTTG GTCCTGGTGA TAACTATGAC AGCTGTCAAA GATGCCACAG 
     1321 ATGACTATTT TCGCCACAAG AGTGATAATC AAGTGAATAA TCGGCAGTCT GAAGTGCTCA 
     1381 TCAACAGCAA ACTGCAGAAT GAAAAATGGA TGAATGTCAA AGTGGGAGAC ATCATTAAAT 
     1441 TAGAAAATAA CCAATTTGTT GCTGCTGATT TACTTCTCCT ATCAAGTAGT GAGCCACATG 
     1501 GTCTCTGTTA TGTTGAAACT GCTGAGCTTG ATGGGGAAAC GAACCTAAAA GTCCGCCATG 
     1561 CACTATCAGT TACTTCAGAA CTTGGAGCAG ATATCAGCAG ACTTGCAGGG TTTGATGGGA 
     1621 TTGTTGTCTG TGAGGTGCCT AACAACAAGT TAGATAAATT CATGGGAATC CTTTCTTGGA 
     1681 AAGACAGCAA GCATTCCCTC AACAATGAGA AGATAATCCT GAGAGGCTGC ATCCTGAGAA 
     1741 ATACCAGCTG GTGTTTTGGA ATGGTTATTT TTGCAGGTCC TGACACTAAA CTAATGCAGA 
     1801 ATAGTGGTAA GACAAAGTTT AAAAGGACAA GCATTGATAG ATTGATGAAT ACTCTAGTAC 
     1861 TATGGATTTT TGGGTTTCTG ATATGCTTGG GAATTATTCT TGCAATAGGA AATTCAATCT 
     1921 GGGAGAGTCA AACTGGGGAC CAATTCAGAA CTTTCCTCTT TTGGAATGAA GGAGAGAAGA 
     1981 GCTCTGTGTT CTCCGGATTC TTAACATTCT GGTCATATAT TATTATTCTC AATACAGTTG 
     2041 TACCCATTTC CTTATATGTG AGTGTGGAAG TAATTCGTCT AGGACACAGT TATTTTATAA 
     2101 ACTGGGACCG GAAGATGTAT TATTCTCGAA AAGCAATACC TGCAGTGGCT CGAACGACCA 
     2161 CGCTCAATGA GGAACTGGGG CAGATTGAGT ACATTTTCTC CGACAAAACG GGTACCCTCA 
     2221 CTCAAAACAT CATGACCTTT AAAAGATGTT CCATTAATGG GAGAATCTAT GGTGAAGTAC 
     2281 ATGATGACCT GGATCAGAAG ACAGAAATAA CTCAGGAAAA AGAGCCTGTG GATTTCTCAG 
     2341 TCAAATCTCA AGCGGATAGA GAATTTCAGT TCTTTGACCA CCATCTGATG GAATCCATTA 
     2401 AAATGGGTGA TCCCAAAGTT CATGAATTCC TTAGGTTACT TGCTCTCTGC CACACTGTAA 
     2461 TGTCAGAAGA GAATAGCGCA GGAGAGCTGA TTTACCAAGT TCAGTCACCT GATGAAGGGG 
     2521 CTCTAGTGAC TGCCGCTAGA AATTTTGGGT TCATTTTTAA ATCCCGGACC CCAGAGACCA 
     2581 TAACAATAGA AGAATTGGGA ACACTAGTTA CTTATCAATT ACTTGCCTTT TTGGATTTCA 
     2641 ACAACACCAG AAAAAGGATG TCTGTCATAG TTCGAAACCC AGAAGGACAG ATAAAGCTTT 
     2701 ATTCCAAAGG AGCAGATACT ATTCTGTTTG AAAAACTTCA TCCTTCCAAT GAAGTCCTTT 
     2761 TGTCTTTGAC GTCAGACCAC CTCAGTGAAT TTGCAGGGGA AGGCCTTCGG ACCTTGGCCA 
     2821 TCGCATACAG AGACCTGGAT GACAAGTACT TTAAAGAGTG GCATAAGATG CTTGAAGATG 
     2881 CGAATGCTGC CACAGAAGAG AGGGATGAAC GAATAGCTGG GCTATATGAA GAAATTGAAA 
     2941 GAGATTTGAT GCTACTAGGT GCCACTGCTG TAGAAGATAA GTTACAGGAG GGTGTTATTG 
     3001 AAACAGTTAC AAGTTTATCA CTAGCCAATA TTAAGATCTG GGTCCTAACA GGAGACAAAC 
     3061 AAGAAACTGC CATCAACATC GGTTATGCCT GCAACATGCT GACTGACGAC ATGAATGATG 
     3121 TGTTTGTGAT AGCAGGGAAT AATGCTGTGG AAGTGAGAGA AGAACTCAGG AAAGCAAAAC 
     3181 AAAATTTGTT TGGACAAAAC AGAAATTTTT CCAATGGCCA TGTAGTTTGT GAAAAAAAGC 
     3241 AGCAGCTGGA GTTGGATTCT ATTGTAGAAG AAACCATAAC AGGAGATTAT GCCTTAATCA 
     3301 TAAATGGCCA CAGTTTGGCT CATGCCCTAG AAAGTGATGT CAAGAATGAT CTCCTAGAAC 
     3361 TTGCTTGCAT GTGTAAGACT GTAATTTGCT GCAGGGTCAC TCCACTCCAG AAAGCCCAAG 
     3421 TGGTAGAGCT GGTGAAGAAG TACAGAAATG CTGTTACTTT GGCCATTGGT GATGGAGCCA 
     3481 ATGATGTCAG CATGATTAAA AGTGCTCACA TTGGTGTTGG CATCAGCGGC CAGGAAGGAT 
     3541 TGCAAGCAGT CTTAGCCAGC GACTATTCAT TTGCACAGTT TAGATATCTC CAAAGGCTTC 
     3601 TCCTTGTTCA TGGAAGGTGG TCTTATTTCC GAATGTGCAA ATTCTTATGC TATTTCTTCT 
     3661 ATAAGAATTT TGCATTTACA CTTGTGCATT TCTGGTTTGG TTTCTTCTGT GGTTTCTCAG 
     3721 CCCAGACTGT TTATGACCAG TGGTTCATCA CCCTTTTTAA CATTGTTTAC ACATCACTGC 
     3781 CTGTTTTAGC CATGGGGATT TTTGACCAGG ATGTGAGTGA CCAGAACAGC GTGGACTGTC 
     3841 CCCAGCTCTA CAAACCAGGA CAGCTGAATC TGCTTTTTAA CAAGCGTAAA TTTTTCATTT 
     3901 GCGTGTTGCA TGGAATCTAC ACCTCATTAG TCCTTTTCTT CATCCCCTAT GGGGCCTTTT 
     3961 ACAACGTGGC TGGAGAAGAT GGGCAACATA TTGCTGACTA CCAGTCCTTT GCAGTTACCA 
     4021 TGGCCACATC TTTGGTCATT GTGGTCAGTG TGCAGATAGC CTTGGATACC AGTTACTGGA 
     4081 CTTTCATTAA TCACGTCTTC ATCTGGGGGA GCATTGCCAT TTATTTCTCC ATTTTATTTA 
     4141 CAATGCACAG TAATGGCATC TTTGGCATCT TCCCAAACCA GTTTCCATTT GTTGGTAATG 
     4201 CACGACATTC CCTGACCCAG AAGTGCATCT GGCTTGTAAT TCTCTTAACA ACAGTGGCTT 
     4261 CAGTTATGCC AGTGGTGGCA TTCAGATTTT TGAAGGTGGA TTTATACCCA ACCCTGAGTG 
     4321 ATCAGATCCG CCGGTGGCAG AAGGCTCAAA AGAAGGCAAG GCCTCCAAGT AGCCGAAGGC 
     4381 CTCGGACCCG CAGGTCAAGC TCAAGAAGGT CTGGATATGC TTTTGCTCAC CAAGAAGGCT 
     4441 ATGGAGAGCT TATCACATCT GGAAAAAATA TGCGAGCTAA AAATCCACCC CCAACATCAG 
     4501 GGCTGGAAAA GACACATTAT AATAGCACTA GCTGGATTGA AAATTTATGT AAGAAAACCA 
     4561 CAGACACCGT GAGCAGCTTT AGCCAGGATA AAACAGTGAA ACTGACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//