LOCUS       RC216075                9141 bp    DNA     circular  SYN 
DEFINITION  BLM (Myc-DDK-tagged)-Human Bloom syndrome, RecQ helicase-like (BLM)
ACCESSION   NM_000057
VERSION     NM_000057.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5298..5389 
                     /label="Myc-DDK"  
     misc_feature    5419..6004 
                     /label="PolyA signal"  
     misc_feature    5476..5495 
                     /label="Restriction Enzyme"  
     misc_feature    6280..6952 
                     /label="ColE1"  
     misc_feature    7382..8176 
                     /label="Kan/Neo"  
     misc_feature    8211..8513 
                     /label="SV40 ori"  
     misc_feature    8599..9076 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    5280..5286 
                     /label="Restriction Enzyme"  
     misc_feature    1029..5280 
                     /label="BLM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCTGCTGTTC CTCAAAATAA TCTACAGGAG CAACTAGAAC 
     1081 GTCACTCAGC CAGAACACTT AATAATAAAT TAAGTCTTTC AAAACCAAAA TTTTCAGGTT 
     1141 TCACTTTTAA AAAGAAAACA TCTTCAGATA ACAATGTATC TGTAACTAAT GTGTCAGTAG 
     1201 CAAAAACACC TGTATTAAGA AATAAAGATG TTAATGTTAC CGAAGACTTT TCCTTCAGTG 
     1261 AACCTCTACC CAACACCACA AATCAGCAAA GGGTCAAGGA CTTCTTTAAA AATGCTCCAG 
     1321 CAGGACAGGA AACACAGAGA GGTGGATCAA AATCATTATT GCCAGATTTC TTGCAGACTC 
     1381 CGAAGGAAGT TGTATGCACT ACCCAAAACA CACCAACTGT AAAGAAATCC CGGGATACTG 
     1441 CTCTCAAGAA ATTAGAATTT AGTTCTTCAC CAGATTCTTT AAGTACCATC AATGATTGGG 
     1501 ATGATATGGA TGACTTTGAT ACTTCTGAGA CTTCAAAATC ATTTGTTACA CCACCCCAAA 
     1561 GTCACTTTGT AAGAGTAAGC ACTGCTCAGA AATCAAAAAA GGGTAAGAGA AACTTTTTTA 
     1621 AAGCACAGCT TTATACAACA AACACAGTAA AGACTGATTT GCCTCCACCC TCCTCTGAAA 
     1681 GCGAGCAAAT AGATTTGACT GAGGAACAGA AGGATGACTC AGAATGGTTA AGCAGCGATG 
     1741 TGATTTGCAT CGATGATGGC CCCATTGCTG AAGTGCATAT AAATGAAGAT GCTCAGGAAA 
     1801 GTGACTCTCT GAAAACTCAT TTGGAAGATG AAAGAGATAA TAGCGAAAAG AAGAAGAATT 
     1861 TGGAAGAAGC TGAATTACAT TCAACTGAGA AAGTTCCATG TATTGAATTT GATGATGATG 
     1921 ATTATGATAC GGATTTTGTT CCACCTTCTC CAGAAGAAAT TATTTCTGCT TCTTCTTCCT 
     1981 CTTCAAAATG CCTTAGTACG TTAAAGGACC TTGACACCTC TGACAGAAAA GAGGATGTTC 
     2041 TTAGCACATC AAAAGATCTT TTGTCAAAAC CTGAGAAAAT GAGTATGCAG GAGCTGAATC 
     2101 CAGAAACCAG CACAGACTGT GACGCTAGAC AGATAAGTTT ACAGCAGCAG CTTATTCATG 
     2161 TGATGGAGCA CATCTGTAAA TTAATTGATA CTATTCCTGA TGATAAACTG AAACTTTTGG 
     2221 ATTGTGGGAA CGAACTGCTT CAGCAGCGGA ACATAAGAAG GAAACTTCTA ACGGAAGTAG 
     2281 ATTTTAATAA AAGTGATGCC AGTCTTCTTG GCTCATTGTG GAGATACAGG CCTGATTCAC 
     2341 TTGATGGCCC TATGGAGGGT GATTCCTGCC CTACAGGGAA TTCTATGAAG GAGTTAAATT 
     2401 TTTCACACCT TCCCTCAAAT TCTGTTTCTC CTGGGGACTG TTTACTGACT ACCACCCTAG 
     2461 GAAAGACAGG ATTCTCTGCC ACCAGGAAGA ATCTTTTTGA AAGGCCTTTA TTCAATACCC 
     2521 ATTTACAGAA GTCCTTTGTA AGTAGCAACT GGGCTGAAAC ACCAAGACTA GGAAAAAAAA 
     2581 ATGAAAGCTC TTATTTCCCA GGAAATGTTC TCACAAGCAC TGCTGTGAAA GATCAGAATA 
     2641 AACATACTGC TTCAATAAAT GACTTAGAAA GAGAAACCCA ACCTTCCTAT GATATTGATA 
     2701 ATTTTGACAT AGATGACTTT GATGATGATG ATGACTGGGA AGACATAATG CATAATTTAG 
     2761 CAGCCAGCAA ATCTTCCACA GCTGCCTATC AACCCATCAA GGAAGGTCGG CCAATTAAAT 
     2821 CAGTATCAGA AAGACTTTCC TCAGCCAAGA CAGACTGTCT TCCAGTGTCA TCTACTGCTC 
     2881 AAAATATAAA CTTCTCAGAG TCAATTCAGA ATTATACTGA CAAGTCAGCA CAAAATTTAG 
     2941 CATCCAGAAA TCTGAAACAT GAGCGTTTCC AAAGTCTTAG TTTTCCTCAT ACAAAGGAAA 
     3001 TGATGAAGAT TTTTCATAAA AAATTTGGCC TGCATAATTT TAGAACTAAT CAGCTAGAGG 
     3061 CGATCAATGC TGCACTGCTT GGTGAAGACT GTTTTATCCT GATGCCGACT GGAGGTGGTA 
     3121 AGAGTTTGTG TTACCAGCTC CCTGCCTGTG TTTCTCCTGG GGTCACTGTT GTCATTTCTC 
     3181 CCTTGAGATC ACTTATCGTA GATCAAGTCC AAAAGCTGAC TTCCTTGGAT ATTCCAGCTA 
     3241 CATATCTGAC AGGTGATAAG ACTGACTCAG AAGCTACAAA TATTTACCTC CAGTTATCAA 
     3301 AAAAAGACCC AATCATAAAA CTTCTATATG TCACTCCAGA AAAGATCTGT GCAAGTAACA 
     3361 GACTCATTTC TACTCTGGAG AATCTCTATG AGAGGAAGCT CTTGGCACGT TTTGTTATTG 
     3421 ATGAAGCACA TTGTGTCAGT CAGTGGGGAC ATGATTTTCG TCAAGATTAC AAAAGAATGA 
     3481 ATATGCTTCG CCAGAAGTTT CCTTCTGTTC CGGTGATGGC TCTTACGGCC ACAGCTAATC 
     3541 CCAGGGTACA GAAGGACATC CTGACTCAGC TGAAGATTCT CAGACCTCAG GTGTTTAGCA 
     3601 TGAGCTTTAA CAGACATAAT CTGAAATACT ATGTATTACC GAAAAAGCCT AAAAAGGTGG 
     3661 CATTTGATTG CCTAGAATGG ATCAGAAAGC ACCACCCATA TGATTCAGGG ATAATTTACT 
     3721 GCCTCTCCAG GCGAGAATGT GACACCATGG CTGACACGTT ACAGAGAGAT GGGCTCGCTG 
     3781 CTCTTGCTTA CCATGCTGGC CTCAGTGATT CTGCCAGAGA TGAAGTGCAG CAGAAGTGGA 
     3841 TTAATCAGGA TGGCTGTCAG GTTATCTGTG CTACAATTGC ATTTGGAATG GGGATTGACA 
     3901 AACCGGACGT GCGATTTGTG ATTCATGCAT CTCTCCCTAA ATCTGTGGAG GGTTACTACC 
     3961 AAGAATCTGG CAGAGCTGGA AGAGATGGGG AAATATCTCA CTGCCTGCTT TTCTATACCT 
     4021 ATCATGATGT GACCAGACTG AAAAGACTTA TAATGATGGA AAAAGATGGA AACCATCATA 
     4081 CAAGAGAAAC TCACTTCAAT AATTTGTATA GCATGGTACA TTACTGTGAA AATATAACGG 
     4141 AATGCAGGAG AATACAGCTT TTGGCCTACT TTGGTGAAAA TGGATTTAAT CCTGATTTTT 
     4201 GTAAGAAACA CCCAGATGTT TCTTGTGATA ATTGCTGTAA AACAAAGGAT TATAAAACAA 
     4261 GAGATGTGAC TGACGATGTG AAAAGTATTG TAAGATTTGT TCAAGAACAT AGTTCATCAC 
     4321 AAGGAATGAG AAATATAAAA CATGTAGGTC CTTCTGGAAG ATTTACTATG AATATGCTGG 
     4381 TCGACATTTT CTTGGGGAGT AAGAGTGCAA AAATCCAGTC AGGTATATTT GGAAAAGGAT 
     4441 CTGCTTATTC ACGACACAAT GCCGAAAGAC TTTTTAAAAA GCTGATACTT GACAAGATTT 
     4501 TGGATGAAGA CTTATATATC AATGCCAATG ACCAGGCGAT CGCTTATGTG ATGCTCGGAA 
     4561 ATAAAGCCCA AACTGTACTA AATGGCAATT TAAAGGTAGA CTTTATGGAA ACAGAAAATT 
     4621 CCAGCAGTGT GAAAAAACAA AAAGCGTTAG TAGCAAAAGT GTCTCAGAGG GAAGAGATGG 
     4681 TTAAAAAATG TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA GTTTTTGGTG 
     4741 TCCATTACTT CAATATTTTT AATACCGTCA CTCTCAAGAA GCTTGCAGAA TCTTTATCTT 
     4801 CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG GAAAAATATG 
     4861 GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA GCTGAAGACA 
     4921 GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC GCTGAGGAGC 
     4981 TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA AATGAAAGGA 
     5041 AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT TCCAGTGGTT 
     5101 CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG AAATCCTCCA 
     5161 GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA GCCAATAGCA 
     5221 AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG CCTTCATATG 
     5281 CATTCTCAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5341 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5401 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5461 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5521 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5581 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5641 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5701 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5761 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5821 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5881 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5941 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6001 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6061 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6121 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6181 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6241 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6301 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6361 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6421 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6481 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6541 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6601 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6661 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6721 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6781 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6841 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6901 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6961 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7021 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7081 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7141 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7201 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7261 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7321 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7381 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7441 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7501 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7561 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7621 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7681 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7741 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7801 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7861 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7921 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7981 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8041 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8101 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8161 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8221 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8281 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8341 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8401 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8461 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8521 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8581 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8641 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8701 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8761 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8821 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8881 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8941 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9001 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9061 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9121 CAAAAATTTA ACGCGAATTT T
//