LOCUS       RC215977                8949 bp    DNA     circular  SYN 
DEFINITION  ADCY9 (Myc-DDK-tagged)-Human adenylate cyclase 9 (ADCY9)
ACCESSION   NM_001116
VERSION     NM_001116.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5104..5195 
                     /label="Myc-DDK"  
     misc_feature    5225..5810 
                     /label="PolyA signal"  
     misc_feature    5282..5301 
                     /label="Restriction Enzyme"  
     misc_feature    6086..6758 
                     /label="ColE1"  
     misc_feature    7188..7982 
                     /label="Kan/Neo"  
     misc_feature    8017..8319 
                     /label="SV40 ori"  
     misc_feature    8405..8882 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5079..5086 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5079 
                     /label="ADCY9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCCCCA CCCCACCAGC AGCTGCTGCA TCACCACAGC ACCGAGGTGA 
     1081 GCTGCGACTC CAGCGGGGAC AGCAACAGCG TGCGCGTCAA GATCAACCCC AAGCAGCTGT 
     1141 CCTCCAACAG CCACCCCAAG CACTGCAAAT ACAGCATCTC CTCTAGCTGC AGCAGCTCTG 
     1201 GGGACTCCGG GGGCGTCCCC CGGCGAGTGG GCGGCGGAGG CCGGCTGCGC AGGCAGAAGA 
     1261 AGCTGCCCCA GCTGTTCGAG AGGGCCTCCA GCCGCTGGTG GGACCCCAAG TTCGACTCGG 
     1321 TGAACCTGGA GGAGGCCTGC CTGGAGCGCT GCTTCCCGCA GACCCAGCGC CGGTTCCGGT 
     1381 ATGCGCTCTT CTACATCGGC TTCGCCTGCC TTCTGTGGAG CATCTATTTT GCGGTCCACA 
     1441 TGAGATCCAG ACTGATCGTC ATGGTCGCCC CCGCGCTGTG CTTCCTCCTG GTGTGTGTGG 
     1501 GCTTCTTTCT GTTTACCTTC ACCAAGCTGT ACGCCCGGCA TTACGCGTGG ACCTCGCTGG 
     1561 CTCTCACCCT GCTGGTGTTC GCCCTGACCC TGGCTGCGCA GTTCCAGGTC TTGACGCCTG 
     1621 TCTCAGGACG CGGCGACAGC TCCAACCTTA CGGCCACAGC CCGGCCCACA GATACTTGCT 
     1681 TATCTCAAGT GGGGAGCTTC TCCATGTGCA TCGAAGTGCT CTTTTTGCTC TATACCGTCA 
     1741 TGCACTTACC TTTGTACCTG AGTTTGTGTC TGGGGGTGGC CTACTCTGTC CTTTTCGAGA 
     1801 CCTTTGGCTA CCATTTCCGG GATGAAGCCT GCTTCCCCTC GCCCGGAGCC GGGGCCCTGC 
     1861 ACTGGGAGCT GCTGAGCAGG GGGCTGCTCC ACGGCTGCAT CCACGCCATC GGGGTCCACC 
     1921 TGTTCGTCAT GTCCCAGGTG AGGTCCAGGA GCACCTTCCT CAAGGTGGGG CAATCCATTA 
     1981 TGCACGGGAA GGACCTGGAA GTGGAAAAAG CCCTCAAAGA GAGGATGATT CATTCCGTGA 
     2041 TGCCAAGAAT CATAGCCGAT GACTTAATGA AGCAGGGAGA TGAGGAGAGT GAGAATTCTG 
     2101 TCAAGAGGCA TGCCACCTCG AGCCCCAAGA ACAGGAAGAA AAAGTCTTCC ATCCAAAAAG 
     2161 CTCCTATAGC CTTCCGCCCT TTTAAGATGC AGCAGATCGA AGAAGTCAGT ATTTTATTTG 
     2221 CAGATATCGT GGGCTTCACC AAGATGAGTG CCAACAAGTC TGCCCACGCC CTGGTGGGTC 
     2281 TCCTGAACGA TCTGTTCGGT CGCTTCGACC GCCTGTGTGA GGAGACCAAG TGTGAGAAAA 
     2341 TCAGCACCCT GGGAGACTGT TACTACTGCG TGGCGGGCTG TCCCGAGCCC CGGGCCGACC 
     2401 ATGCCTACTG CTGCATCGAG ATGGGCCTGG GCATGATCAA GGCCATCGAG CAGTTCTGCC 
     2461 AGGAGAAGAA GGAGATGGTG AACATGAGAG TCGGGGTGCA CACGGGCACC GTCCTTTGCG 
     2521 GCATCCTGGG CATGAGGAGG TTTAAATTTG ACGTGTGGTC CAACGATGTG AACCTGGCCA 
     2581 ATCTCATGGA GCAGCTGGGA GTGGCCGGCA AAGTTCACAT TTCTGAGGCC ACCGCAAAAT 
     2641 ACTTAGATGA CCGGTACGAA ATGGAAGATG GGAAAGTTAT TGAACGGCTG GGCCAGAGCG 
     2701 TGGTTGCTGA CCAGTTGAAA GGTTTGAAGA CATACCTGAT ATCGGGTCAG AGAGCCAAGG 
     2761 AGTCTCGCTG CAGCTGTGCA GAGGCCTTGC TTTCTGGCTT TGAGGTCATT GACGGCTCAC 
     2821 AGGTGTCCTC AGGCCCTAGG GGACAGGGGA CAGCGTCATC AGGGAATGTC AGTGACTTGG 
     2881 CGCAGACTGT CAAAACCTTT GATAACCTTA AGACCTGCCC TTCGTGCGGA ATCACATTTG 
     2941 CTCCCAAATC TGAAGCCGGC GCCGAGGGAG GAGCACCTCA AAACGGCTGC CAAGACGAGC 
     3001 ATAAAAACAG CACCAAGGCT TCTGGAGGAC CTAATCCCAA AACTCAGAAC GGGCTCCTCA 
     3061 GCCCTCCCCA AGAGGAGAAG CTCACCAACA GTCAGACTTC TCTGTGTGAG ATCTTGCAGG 
     3121 AGAAGGGAAG GTGGGCAGGG GTGAGCCTGG ACCAGTCGGC TCTCCTTCCG CTGAGGTTCA 
     3181 AGAACATCCG GGAGAAAACG GACGCCCACT TTGTGGACGT TATCAAAGAA GACAGCCTGA 
     3241 TGAAAGATTA CTTTTTTAAG CCGCCCATTA ATCAGTTCAG CCTGAACTTC CTGGATCAGG 
     3301 AGCTGGAGCG ATCCTACAGG ACCAGCTATC AGGAAGAGGT CATAAAGAAC TCCCCCGTGA 
     3361 AGACGTTTGC TAGTCCCACC TTCAGCTCCC TCCTGGATGT GTTTCTGTCG ACCACAGTGT 
     3421 TTCTGACGCT GTCCACCACC TGCTTCCTGA AGTACGAGGC GGCCACCGTG CCTCCCCCGC 
     3481 CCGCCGCCCT GGCGGTCTTC AGTGCAGCCC TGCTGCTGGA GGTGCTGTCC CTCGCGGTGT 
     3541 CCATCAGGAT GGTGTTCTTC CTGGAGGACG TCATGGCCTG CACCAAGCGC CTGCTGGAGT 
     3601 GGATCGCCGG CTGGCTACCA CGTCACTGCA TCGGGGCCAT CCTGGTGTCG CTTCCCGCAC 
     3661 TGGCCGTCTA CTCCCATGTC ACCTCCGAAT ATGAGACCAA CATACACTTC CCAGTGTTCA 
     3721 CAGGCTCGGC CGCGCTGATT GCCGTCGTGC ACTACTGTAA CTTCTGCCAG CTCAGCTCCT 
     3781 GGATGAGGTC CTCCCTCGCC ACCGTCGTGG GGGCCGGGCC GCTGCTCCTG CTCTACGTCT 
     3841 CCCTGTGCCC AGACAGTTCT GTATTAACTT CGCCCCTTGA CGCAGTACAG AATTTCAGTT 
     3901 CCGAGAGGAA CCCGTGCAAT AGTTCGGTGC CGCGTGACCT CCGGCGGCCC GCCAGCCTCA 
     3961 TCGGCCAGGA GGTGGTTCTC GTCTTCTTTC TCCTGCTCTT GTTGGTCTGG TTCCTGAATC 
     4021 GCGAATTTGA AGTCAGCTAC CGCCTCCACT ACCACGGAGA CGTGGAAGCG GATCTTCACC 
     4081 GCACCAAGAT CCAGAGCATG CGGGACCAGG CAGACTGGCT GCTGAGGAAC ATCATCCCCT 
     4141 ACCACGTGGC TGAGCAGCTG AAGGTGTCCC AGACCTACTC CAAGAACCAT GACAGCGGAG 
     4201 GGGTGATCTT CGCCAGCATC GTCAACTTCA GCGAGTTCTA CGAGGAGAAC TACGAGGGCG 
     4261 GCAAGGAGTG CTACCGGGTC CTCAACGAGC TCATCGGGGA CTTTGACGAG CTCCTAAGCA 
     4321 AGCCGGACTA CAGCAGCATC GAGAAGATCA AGACCATCGG AGCCACGTAC ATGGCGGCGT 
     4381 CAGGGCTGAA CACCGCGCAG GCCCAGGACG GCAGCCACCC GCAGGAGCAC CTGCAGATCC 
     4441 TGTTCGAGTT CGCCAAGGAG ATGATGCGCG TGGTGGACGA CTTCAACAAC AACATGCTGT 
     4501 GGTTCAACTT CAAGCTCCGC GTCGGCTTCA ACCATGGGCC CCTCACGGCC GGGGTCATCG 
     4561 GCACCACCAA GCTGCTGTAC GACATCTGGG GAGACACCGT CAACATCGCC AGCAGGATGG 
     4621 ACACCACCGG CGTGGAGTGC CGCATCCAGG TGAGCGAAGA GAGCTACCGC GTCTTGAGCA 
     4681 AGATGGGCTA TGACTTCGAC TACAGAGGGA CCGTGAATGT CAAGGGGAAA GGCCAGATGA 
     4741 AGACCTACCT GTACCCAAAG TGCACGGATC ACAGGGTCAT CCCACAGCAC CAGCTGTCCA 
     4801 TCTCCCCAGA CATCCGCGTC CAGGTGGATG GCAGCATCGG ACGGTCTCCC ACAGACGAGA 
     4861 TTGCCAACCT GGTGCCTTCT GTCCAGTATG TGGACAAGAC ATCTCTGGGT TCTGACAGCA 
     4921 GCACGCAGGC CAAGGATGCC CACCTGTCCC CCAAGAGACC GTGGAAGGAG CCCGTCAAAG 
     4981 CCGAAGAAAG GGGTCGATTT GGCAAAGCCA TAGAGAAAGA CGACTGTGAC GAAACAGGAA 
     5041 TAGAAGAAGC CAACGAACTC ACCAAGCTCA ACGTTTCAAA GAGTGTGAGC GGACCGACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//