LOCUS       RC215928                8358 bp    DNA     circular  SYN 
DEFINITION  KCNH2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 1
ACCESSION   NM_000238
VERSION     NM_000238.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4515..4606 
                     /label="Myc-DDK"  
     misc_feature    4636..5221 
                     /label="PolyA signal"  
     misc_feature    4693..4712 
                     /label="Restriction Enzyme"  
     misc_feature    5497..6169 
                     /label="ColE1"  
     misc_feature    6599..7393 
                     /label="Kan/Neo"  
     misc_feature    7428..7730 
                     /label="SV40 ori"  
     misc_feature    7816..8293 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4497..4503 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4497 
                     /label="KCNH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGCGG AGGGGCCACG TCGCGCCGCA GAACACCTTC CTGGACACCA 
     1081 TCATCCGCAA GTTTGAGGGC CAGAGCCGTA AGTTCATCAT CGCCAACGCT CGGGTGGAGA 
     1141 ACTGCGCCGT CATCTACTGC AACGACGGCT TCTGCGAGCT GTGCGGCTAC TCGCGGGCCG 
     1201 AGGTGATGCA GCGACCCTGC ACCTGCGACT TCCTGCACGG GCCGCGCACG CAGCGCCGCG 
     1261 CTGCCGCGCA GATCGCGCAG GCACTGCTGG GCGCCGAGGA GCGCAAAGTG GAAATCGCCT 
     1321 TCTACCGGAA AGATGGGAGC TGCTTCCTAT GTCTGGTGGA TGTGGTGCCC GTGAAGAACG 
     1381 AGGATGGGGC TGTCATCATG TTCATCCTCA ATTTCGAGGT GGTGATGGAG AAGGACATGG 
     1441 TGGGGTCCCC GGCTCATGAC ACCAACCACC GGGGCCCCCC CACCAGCTGG CTGGCCCCAG 
     1501 GCCGCGCCAA GACCTTCCGC CTGAAGCTGC CCGCGCTGCT GGCGCTGACG GCCCGGGAGT 
     1561 CGTCGGTGCG GTCGGGCGGC GCGGGCGGCG CGGGCGCCCC GGGGGCCGTG GTGGTGGACG 
     1621 TGGACCTGAC GCCCGCGGCA CCCAGCAGCG AGTCGCTGGC CCTGGACGAA GTGACAGCCA 
     1681 TGGACAACCA CGTGGCAGGG CTCGGGCCCG CGGAGGAGCG GCGTGCGCTG GTGGGTCCCG 
     1741 GCTCTCCGCC CCGCAGCGCG CCCGGCCAGC TCCCATCGCC CCGGGCGCAC AGCCTCAACC 
     1801 CCGACGCCTC GGGCTCCAGC TGCAGCCTGG CCCGGACGCG CTCCCGAGAA AGCTGCGCCA 
     1861 GCGTGCGCCG CGCCTCGTCG GCCGACGACA TCGAGGCCAT GCGCGCCGGG GTGCTGCCCC 
     1921 CGCCACCGCG CCACGCCAGC ACCGGGGCCA TGCACCCACT GCGCAGCGGC TTGCTCAACT 
     1981 CCACCTCGGA CTCCGACCTC GTGCGCTACC GCACCATTAG CAAGATTCCC CAAATCACCC 
     2041 TCAACTTTGT GGACCTCAAG GGCGACCCCT TCTTGGCTTC GCCCACCAGT GACCGTGAGA 
     2101 TCATAGCACC TAAGATAAAG GAGCGAACCC ACAATGTCAC TGAGAAGGTC ACCCAGGTCC 
     2161 TGTCCCTGGG CGCCGACGTG CTGCCTGAGT ACAAGCTGCA GGCACCGCGC ATCCACCGCT 
     2221 GGACCATCCT GCATTACAGC CCCTTCAAGG CCGTGTGGGA CTGGCTCATC CTGCTGCTGG 
     2281 TCATCTACAC GGCTGTCTTC ACACCCTACT CGGCTGCCTT CCTGCTGAAG GAGACGGAAG 
     2341 AAGGCCCGCC TGCTACCGAG TGTGGCTACG CCTGCCAGCC GCTGGCTGTG GTGGACCTCA 
     2401 TCGTGGACAT CATGTTCATT GTGGACATCC TCATCAACTT CCGCACCACC TACGTCAATG 
     2461 CCAACGAGGA GGTGGTCAGC CACCCCGGCC GCATCGCCGT CCACTACTTC AAGGGCTGGT 
     2521 TCCTCATCGA CATGGTGGCC GCCATCCCCT TCGACCTGCT CATCTTCGGC TCTGGCTCTG 
     2581 AGGAGCTGAT CGGGCTGCTG AAGACTGCGC GGCTGCTGCG GCTGGTGCGC GTGGCGCGGA 
     2641 AGCTGGATCG CTACTCAGAG TACGGCGCGG CCGTGCTGTT CTTGCTCATG TGCACCTTTG 
     2701 CGCTCATCGC GCACTGGCTA GCCTGCATCT GGTACGCCAT CGGCAACATG GAGCAGCCAC 
     2761 ACATGGACTC ACGCATCGGC TGGCTGCACA ACCTGGGCGA CCAGATAGGC AAACCCTACA 
     2821 ACAGCAGCGG CCTGGGCGGC CCCTCCATCA AGGACAAGTA TGTGACGGCG CTCTACTTCA 
     2881 CCTTCAGCAG CCTCACCAGT GTGGGCTTCG GCAACGTCTC TCCCAACACC AACTCAGAGA 
     2941 AGATCTTCTC CATCTGCGTC ATGCTCATTG GCTCCCTCAT GTATGCTAGC ATCTTCGGCA 
     3001 ACGTGTCGGC CATCATCCAG CGGCTGTACT CGGGCACAGC CCGCTACCAC ACACAGATGC 
     3061 TGCGGGTGCG GGAGTTCATC CGCTTCCACC AGATCCCCAA TCCCCTGCGC CAGCGCCTCG 
     3121 AGGAGTACTT CCAGCACGCC TGGTCCTACA CCAACGGCAT CGACATGAAC GCGGTGCTGA 
     3181 AGGGCTTCCC TGAGTGCCTG CAGGCTGACA TCTGCCTGCA CCTGAACCGC TCACTGCTGC 
     3241 AGCACTGCAA ACCCTTCCGA GGGGCCACCA AGGGCTGCCT TCGGGCCCTG GCCATGAAGT 
     3301 TCAAGACCAC ACATGCACCG CCAGGGGACA CACTGGTGCA TGCTGGGGAC CTGCTCACCG 
     3361 CCCTGTACTT CATCTCCCGG GGCTCCATCG AGATCCTGCG GGGCGACGTC GTCGTGGCCA 
     3421 TCCTGGGGAA GAATGACATC TTTGGGGAGC CTCTGAACCT GTATGCAAGG CCTGGCAAGT 
     3481 CGAACGGGGA TGTGCGGGCC CTCACCTACT GTGACCTACA CAAGATCCAT CGGGACGACC 
     3541 TGCTGGAGGT GCTGGACATG TACCCTGAGT TCTCCGACCA CTTCTGGTCC AGCCTGGAGA 
     3601 TCACCTTCAA CCTGCGAGAT ACCAACATGA TCCCGGGCTC CCCCGGCAGT ACGGAGTTAG 
     3661 AGGGTGGCTT CAGTCGGCAA CGCAAGCGCA AGTTGTCCTT CCGCAGGCGC ACGGACAAGG 
     3721 ACACGGAGCA GCCAGGGGAG GTGTCGGCCT TGGGGCCGGG CCGGGCGGGG GCAGGGCCGA 
     3781 GTAGCCGGGG CCGGCCGGGG GGGCCGTGGG GGGAGAGCCC GTCCAGTGGC CCCTCCAGCC 
     3841 CTGAGAGCAG TGAGGATGAG GGCCCAGGCC GCAGCTCCAG CCCCCTCCGC CTGGTGCCCT 
     3901 TCTCCAGCCC CAGGCCCCCC GGAGAGCCGC CGGGTGGGGA GCCCCTGATG GAGGACTGCG 
     3961 AGAAGAGCAG CGACACTTGC AACCCCCTGT CAGGCGCCTT CTCAGGAGTG TCCAACATTT 
     4021 TCAGCTTCTG GGGGGACAGT CGGGGCCGCC AGTACCAGGA GCTCCCTCGA TGCCCCGCCC 
     4081 CCACCCCCAG CCTCCTCAAC ATCCCCCTCT CCAGCCCGGG TCGGCGGCCC CGGGGCGACG 
     4141 TGGAGAGCAG GCTGGATGCC CTCCAGCGCC AGCTCAACAG GCTGGAGACC CGGCTGAGTG 
     4201 CAGACATGGC CACTGTCCTG CAGCTGCTAC AGAGGCAGAT GACGCTGGTC CCGCCCGCCT 
     4261 ACAGTGCTGT GACCACCCCG GGGCCTGGCC CCACTTCCAC ATCCCCGCTG TTGCCCGTCA 
     4321 GCCCCCTCCC CACCCTCACC TTGGACTCGC TTTCTCAGGT TTCCCAGTTC ATGGCGTGTG 
     4381 AGGAGCTGCC CCCGGGGGCC CCAGAGCTTC CCCAAGAAGG CCCCACACGA CGCCTCTCCC 
     4441 TACCGGGCCA GCTGGGGGCC CTCACCTCCC AGCCCCTGCA CAGACACGGC TCGGACCCGG 
     4501 GCAGTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4561 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4621 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4681 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4741 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4801 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4861 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4921 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4981 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5041 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5101 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5161 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5221 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5281 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5341 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5401 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5461 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5521 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5581 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5641 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5701 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5761 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5821 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5881 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5941 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6001 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6061 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6121 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6181 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6241 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6301 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6361 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6421 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6481 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6541 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6601 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6661 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6721 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6781 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6841 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6901 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6961 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7021 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7081 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7141 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7201 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7261 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7321 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7381 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7441 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7501 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7561 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7801 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7861 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7921 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7981 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8041 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8101 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8161 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8221 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8281 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8341 AAATTTAACG CGAATTTT
//