LOCUS       RC215873L1                9588 bp    DNA     circular  SYN 
DEFINITION  RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1
ACCESSION   NM_002895
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7877..8057 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8785..9444 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8216..8784 
                     /label="ColE1"  
     misc_feature    6752..6772 
                     /label="LR50 primer"  
     misc_feature    6549..6620 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6761..7352 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6524..6531 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6524 
                     /label="RBL1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTCGAGGAC AAGCCCCACG CTGAGGGGGC 
     3361 GGCGGTGGTC GCCGCAGCCG GGGAGGCGCT ACAGGCCCTG TGCCAGGAGC TGAACCTGGA 
     3421 CGAGGGGAGC GCGGCCGAAG CCCTGGACGA CTTTACTGCC ATCCGAGGCA ACTACAGCCT 
     3481 AGAGGGAGAA GTTACACACT GGTTGGCATG TTCATTATAT GTTGCATGCC GCAAAAGCAT 
     3541 TATTCCCACG GTTGGAAAGG GTATCATGGA AGGCAACTGT GTTTCACTTA CCAGAATACT 
     3601 ACGTTCAGCT AAATTAAGTT TAATACAATT TTTTAGTAAA ATGAAGAAAT GGATGGACAT 
     3661 GTCAAATCTA CCACAAGAAT TTCGTGAACG TATAGAAAGG CTAGAGAGAA ATTTTGAGGT 
     3721 GTCTACTGTA ATATTCAAAA AATATGAGCC AATTTTTTTA GATATATTTC AAAATCCATA 
     3781 TGAAGAACCA CCAAAGTTAC CACGAAGCCG GAAGCAGAGG AGGATTCCTT GCAGTGTTAA 
     3841 GGATCTGTTT AATTTCTGTT GGACACTTTT TGTTTATACT AAGGGTAATT TTCGGATGAT 
     3901 TGGGGATGAC TTAGTAAACT CTTATCATTT ACTTCTATGC TGCTTGGATC TGATTTTTGC 
     3961 CAATGCGATT ATGTGCCCAA ATAGACAAGA CTTGCTAAAT CCATCATTTA AAGGTTTACC 
     4021 ATCTGATTTT CATACTGCTG ACTTTACGGC TTCTGAAGAG CCACCCTGCA TCATTGCTGT 
     4081 ACTGTGTGAA CTGCATGATG GACTTCTCGT AGAAGCAAAA GGAATAAAGG AGCACTACTT 
     4141 TAAGCCATAT ATTTCAAAAC TCTTTGACAG GAAGATATTA AAAGGAGAAT GCCTCCTGGA 
     4201 CCTTTCAAGT TTTACTGATA ATAGCAAAGC AGTGAATAAG GAGTATGAAG AGTATGTTCT 
     4261 AACTGTTGGT GATTTTGATG AGAGGATCTT TTTGGGAGCA GACGCAGAAG AGGAAATTGG 
     4321 AACACCTCGA AAGTTCACTC GTGACACCCC ATTAGGGAAA CTGACAGCAC AGGCTAATGT 
     4381 GGAGTATAAC CTTCAACAGC ACTTTGAAAA AAAAAGGTCA TTTGCACCTT CTACCCCACT 
     4441 GACCGGACGG AGATATTTAC GAGAAAAAGA AGCAGTCATT ACTCCTGTTG CATCAGCCAC 
     4501 CCAAAGTGTG AGCCGGTTAC AGAGTATTGT GGCTGGTCTG AAAAATGCAC CAAGTGACCA 
     4561 ACTTATAAAT ATTTTTGAAT CTTGTGTGCG TAATCCTGTG GAAAACATTA TGAAAATACT 
     4621 AAAAGGAATA GGAGAGACTT TCTGTCAACA CTATACTCAA TCAACAGATG AACAGCCAGG 
     4681 ATCTCACATA GACTTTGCTG TAAACAGACT AAAGCTGGCA GAAATTTTGT ATTATAAAAT 
     4741 ACTAGAGACT GTAATGGTTC AGGAAACACG AAGACTTCAT GGAATGGACA TGTCAGTTCT 
     4801 TTTAGAGCAA GATATATTTC ATCGTTCCTT GATGGCTTGT TGTTTGGAAA TTGTGCTCTT 
     4861 TGCCTATAGC TCACCTCGTA CTTTTCCTTG GATTATTGAA GTTCTCAACT TGCAACCATT 
     4921 TTACTTTTAT AAGGTTATTG AGGTGGTGAT CCGCTCAGAA GAGGGGCTCT CAAGGGACAT 
     4981 GGTGAAACAC CTAAACAGCA TTGAAGAACA GATTTTGGAG AGTTTAGCAT GGAGTCACGA 
     5041 TTCTGCACTG TGGGAGGCTC TCCAGGTTTC TGCAAACAAA GTTCCTACCT GTGAAGAAGT 
     5101 TATATTCCCA AATAACTTTG AAACAGGAAA TGGAGGAAAT GTGCAGGGAC ATCTTCCCCT 
     5161 GATGCCAATG TCTCCTCTAA TGCACCCAAG AGTCAAGGAA GTTCGAACTG ACAGTGGGAG 
     5221 TCTTCGAAGA GATATGCAAC CATTGTCTCC AATTTCTGTC CATGAACGCT ACAGTTCTCC 
     5281 TACCGCAGGG AGTGCTAAGA GAAGACTCTT TGGAGAGGAC CCCCCAAAGG AAATGCTTAT 
     5341 GGACAAGATC ATAACAGAAG GAACAAAATT GAAAATCGCT CCTTCTTCAA GCATTACTGC 
     5401 TGAAAATGTA TCAATTTTAC CTGGTCAAAC TCTTCTAACA ATGGCCACAG CCCCAGTAAC 
     5461 AGGAACAACA GGACATAAAG TTACAATTCC ATTACATGGT GTCGCAAATG ATGCTGGAGA 
     5521 GATCACACTG ATACCTCTTT CCATGAATAC AAATCAGGAG TCCAAAGTCA AGAGTCCTGT 
     5581 ATCACTTACT GCTCATTCAT TAATTGGTGC TTCTCCAAAA CAGACCAATC TGACTAAAGC 
     5641 ACAAGAGGTA CATTCAACTG GAATAAACAG GCCAAAGAGA ACTGGGTCCT TAGCACTATT 
     5701 TTACAGAAAG GTCTATCATT TGGCAAGTGT ACGCTTACGT GATCTATGTC TAAAACTGGA 
     5761 TGTTTCAAAT GAGTTACGAA GGAAGATATG GACGTGTTTT GAATTCACTT TAGTTCACTG 
     5821 TCCTGATCTA ATGAAAGACA GGCATTTGGA TCAGCTCCTC CTTTGTGCCT TTTATATCAT 
     5881 GGCAAAGGTA ACAAAAGAAG AAAGAACTTT TCAAGAAATT ATGAAAAGTT ATAGGAATCA 
     5941 GCCCCAAGCT AATAGTCACG TATATAGAAG TGTTCTGCTG AAAAGTATTC CAAGAGAAGT 
     6001 TGTGGCATAT AATAAAAATA TAAATGATGA CTTTGAAATG ATAGATTGTG ACTTAGAAGA 
     6061 TGCTACAAAA ACACCTGACT GTTCCAGTGG ACCAGTGAAA GAGGAAAGAG GTGATCTTAT 
     6121 AAAATTTTAC AATACAATAT ATGTAGGAAG AGTGAAGTCA TTTGCACTGA AATACGACTT 
     6181 GGCGAATCAG GACCATATGA TGGATGCTCC ACCACTCTCT CCTTTTCCAC ATATTAAACA 
     6241 ACAGCCAGGC TCACCACGCC GCATTTCCCA GCAGCACTCC ATTTATATTT CCCCGCACAA 
     6301 GAATGGGTCA GGCCTTACAC CAAGAAGCGC TCTGCTGTAC AAGTTCAATG GCAGCCCTTC 
     6361 TAAGAGTTTG AAAGATATCA ACAACATGAT AAGGCAAGGT GAGCAGAGAA CCAAGAAGCG 
     6421 AGTAATAGCC ATCGATAGTG ATGCAGAATC CCCTGCCAAA CGCGTCTGTC AAGAAAATGA 
     6481 TGACGTTTTA CTGAAACGAC TACAGGATGT TGTCAGTGAA AGAGCAAATC ATAGCGGACC 
     6541 GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     6601 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6661 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6721 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6781 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6841 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6901 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6961 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     7021 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     7081 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     7141 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     7201 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     7261 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     7321 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     7381 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     7441 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     7501 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     7561 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     7621 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7681 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7741 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7801 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7861 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7921 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7981 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     8041 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     8101 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     8161 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     8221 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     8281 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     8341 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     8401 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     8461 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     8521 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     8581 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     8641 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8701 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8761 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8821 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8881 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8941 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     9001 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     9061 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     9121 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     9181 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     9241 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     9301 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     9361 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     9421 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     9481 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9541 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//