LOCUS       RC215873                8094 bp    DNA     circular  SYN 
DEFINITION  RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 1
ACCESSION   NM_002895
VERSION     NM_002895.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4249..4340 
                     /label="Myc-DDK"  
     misc_feature    4370..4955 
                     /label="PolyA signal"  
     misc_feature    4427..4446 
                     /label="Restriction Enzyme"  
     misc_feature    5231..5903 
                     /label="ColE1"  
     misc_feature    6333..7127 
                     /label="Kan/Neo"  
     misc_feature    7162..7464 
                     /label="SV40 ori"  
     misc_feature    7550..8027 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4231 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="RBL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCGAGGAC AAGCCCCACG CTGAGGGGGC GGCGGTGGTC GCCGCAGCCG 
     1081 GGGAGGCGCT ACAGGCCCTG TGCCAGGAGC TGAACCTGGA CGAGGGGAGC GCGGCCGAAG 
     1141 CCCTGGACGA CTTTACTGCC ATCCGAGGCA ACTACAGCCT AGAGGGAGAA GTTACACACT 
     1201 GGTTGGCATG TTCATTATAT GTTGCATGCC GCAAAAGCAT TATTCCCACG GTTGGAAAGG 
     1261 GTATCATGGA AGGCAACTGT GTTTCACTTA CCAGAATACT ACGTTCAGCT AAATTAAGTT 
     1321 TAATACAATT TTTTAGTAAA ATGAAGAAAT GGATGGACAT GTCAAATCTA CCACAAGAAT 
     1381 TTCGTGAACG TATAGAAAGG CTAGAGAGAA ATTTTGAGGT GTCTACTGTA ATATTCAAAA 
     1441 AATATGAGCC AATTTTTTTA GATATATTTC AAAATCCATA TGAAGAACCA CCAAAGTTAC 
     1501 CACGAAGCCG GAAGCAGAGG AGGATTCCTT GCAGTGTTAA GGATCTGTTT AATTTCTGTT 
     1561 GGACACTTTT TGTTTATACT AAGGGTAATT TTCGGATGAT TGGGGATGAC TTAGTAAACT 
     1621 CTTATCATTT ACTTCTATGC TGCTTGGATC TGATTTTTGC CAATGCGATT ATGTGCCCAA 
     1681 ATAGACAAGA CTTGCTAAAT CCATCATTTA AAGGTTTACC ATCTGATTTT CATACTGCTG 
     1741 ACTTTACGGC TTCTGAAGAG CCACCCTGCA TCATTGCTGT ACTGTGTGAA CTGCATGATG 
     1801 GACTTCTCGT AGAAGCAAAA GGAATAAAGG AGCACTACTT TAAGCCATAT ATTTCAAAAC 
     1861 TCTTTGACAG GAAGATATTA AAAGGAGAAT GCCTCCTGGA CCTTTCAAGT TTTACTGATA 
     1921 ATAGCAAAGC AGTGAATAAG GAGTATGAAG AGTATGTTCT AACTGTTGGT GATTTTGATG 
     1981 AGAGGATCTT TTTGGGAGCA GACGCAGAAG AGGAAATTGG AACACCTCGA AAGTTCACTC 
     2041 GTGACACCCC ATTAGGGAAA CTGACAGCAC AGGCTAATGT GGAGTATAAC CTTCAACAGC 
     2101 ACTTTGAAAA AAAAAGGTCA TTTGCACCTT CTACCCCACT GACCGGACGG AGATATTTAC 
     2161 GAGAAAAAGA AGCAGTCATT ACTCCTGTTG CATCAGCCAC CCAAAGTGTG AGCCGGTTAC 
     2221 AGAGTATTGT GGCTGGTCTG AAAAATGCAC CAAGTGACCA ACTTATAAAT ATTTTTGAAT 
     2281 CTTGTGTGCG TAATCCTGTG GAAAACATTA TGAAAATACT AAAAGGAATA GGAGAGACTT 
     2341 TCTGTCAACA CTATACTCAA TCAACAGATG AACAGCCAGG ATCTCACATA GACTTTGCTG 
     2401 TAAACAGACT AAAGCTGGCA GAAATTTTGT ATTATAAAAT ACTAGAGACT GTAATGGTTC 
     2461 AGGAAACACG AAGACTTCAT GGAATGGACA TGTCAGTTCT TTTAGAGCAA GATATATTTC 
     2521 ATCGTTCCTT GATGGCTTGT TGTTTGGAAA TTGTGCTCTT TGCCTATAGC TCACCTCGTA 
     2581 CTTTTCCTTG GATTATTGAA GTTCTCAACT TGCAACCATT TTACTTTTAT AAGGTTATTG 
     2641 AGGTGGTGAT CCGCTCAGAA GAGGGGCTCT CAAGGGACAT GGTGAAACAC CTAAACAGCA 
     2701 TTGAAGAACA GATTTTGGAG AGTTTAGCAT GGAGTCACGA TTCTGCACTG TGGGAGGCTC 
     2761 TCCAGGTTTC TGCAAACAAA GTTCCTACCT GTGAAGAAGT TATATTCCCA AATAACTTTG 
     2821 AAACAGGAAA TGGAGGAAAT GTGCAGGGAC ATCTTCCCCT GATGCCAATG TCTCCTCTAA 
     2881 TGCACCCAAG AGTCAAGGAA GTTCGAACTG ACAGTGGGAG TCTTCGAAGA GATATGCAAC 
     2941 CATTGTCTCC AATTTCTGTC CATGAACGCT ACAGTTCTCC TACCGCAGGG AGTGCTAAGA 
     3001 GAAGACTCTT TGGAGAGGAC CCCCCAAAGG AAATGCTTAT GGACAAGATC ATAACAGAAG 
     3061 GAACAAAATT GAAAATCGCT CCTTCTTCAA GCATTACTGC TGAAAATGTA TCAATTTTAC 
     3121 CTGGTCAAAC TCTTCTAACA ATGGCCACAG CCCCAGTAAC AGGAACAACA GGACATAAAG 
     3181 TTACAATTCC ATTACATGGT GTCGCAAATG ATGCTGGAGA GATCACACTG ATACCTCTTT 
     3241 CCATGAATAC AAATCAGGAG TCCAAAGTCA AGAGTCCTGT ATCACTTACT GCTCATTCAT 
     3301 TAATTGGTGC TTCTCCAAAA CAGACCAATC TGACTAAAGC ACAAGAGGTA CATTCAACTG 
     3361 GAATAAACAG GCCAAAGAGA ACTGGGTCCT TAGCACTATT TTACAGAAAG GTCTATCATT 
     3421 TGGCAAGTGT ACGCTTACGT GATCTATGTC TAAAACTGGA TGTTTCAAAT GAGTTACGAA 
     3481 GGAAGATATG GACGTGTTTT GAATTCACTT TAGTTCACTG TCCTGATCTA ATGAAAGACA 
     3541 GGCATTTGGA TCAGCTCCTC CTTTGTGCCT TTTATATCAT GGCAAAGGTA ACAAAAGAAG 
     3601 AAAGAACTTT TCAAGAAATT ATGAAAAGTT ATAGGAATCA GCCCCAAGCT AATAGTCACG 
     3661 TATATAGAAG TGTTCTGCTG AAAAGTATTC CAAGAGAAGT TGTGGCATAT AATAAAAATA 
     3721 TAAATGATGA CTTTGAAATG ATAGATTGTG ACTTAGAAGA TGCTACAAAA ACACCTGACT 
     3781 GTTCCAGTGG ACCAGTGAAA GAGGAAAGAG GTGATCTTAT AAAATTTTAC AATACAATAT 
     3841 ATGTAGGAAG AGTGAAGTCA TTTGCACTGA AATACGACTT GGCGAATCAG GACCATATGA 
     3901 TGGATGCTCC ACCACTCTCT CCTTTTCCAC ATATTAAACA ACAGCCAGGC TCACCACGCC 
     3961 GCATTTCCCA GCAGCACTCC ATTTATATTT CCCCGCACAA GAATGGGTCA GGCCTTACAC 
     4021 CAAGAAGCGC TCTGCTGTAC AAGTTCAATG GCAGCCCTTC TAAGAGTTTG AAAGATATCA 
     4081 ACAACATGAT AAGGCAAGGT GAGCAGAGAA CCAAGAAGCG AGTAATAGCC ATCGATAGTG 
     4141 ATGCAGAATC CCCTGCCAAA CGCGTCTGTC AAGAAAATGA TGACGTTTTA CTGAAACGAC 
     4201 TACAGGATGT TGTCAGTGAA AGAGCAAATC ATAGCGGACC GACGCGTACG CGGCCGCTCG 
     4261 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4321 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4381 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4441 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4501 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4561 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4621 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4681 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4741 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4801 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4861 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4921 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4981 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5041 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5101 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5161 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5221 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5281 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5341 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5401 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5461 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5521 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5581 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5641 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5701 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5761 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5821 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5881 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5941 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6001 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6061 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6121 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6181 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6241 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6301 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6361 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6421 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6481 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6541 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6601 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6661 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6721 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6781 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6841 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6901 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6961 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7021 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7081 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7141 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7201 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7261 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7321 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7501 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7561 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7621 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7681 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7741 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7801 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7861 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7921 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7981 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8041 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//