LOCUS       RC215785                9429 bp    DNA     circular  SYN 
DEFINITION  AGL (Myc-DDK-tagged)-Human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant 6
ACCESSION   NM_000646
VERSION     NM_000646.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5586..5677 
                     /label="Myc-DDK"  
     misc_feature    5707..6292 
                     /label="PolyA signal"  
     misc_feature    5764..5783 
                     /label="Restriction Enzyme"  
     misc_feature    6568..7240 
                     /label="ColE1"  
     misc_feature    7670..8464 
                     /label="Kan/Neo"  
     misc_feature    8499..8801 
                     /label="SV40 ori"  
     misc_feature    8887..9364 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5568..5574 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5568 
                     /label="AGL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCAATT CTGAGCATTA ATTTATTTAT TGGGTATGAG CTACAGTTCC 
     1081 GATTAGGCCC AACTTTACAG GGAAAAGCAG TTACCGTGTA TACAAATTAC CCATTTCCTG 
     1141 GAGAAACATT TAATAGAGAA AAATTCCGTT CTCTGGATTG GGAAAATCCA ACAGAAAGAG 
     1201 AAGATGATTC TGATAAATAC TGTAAACTTA ATCTGCAACA ATCTGGTTCA TTTCAGTATT 
     1261 ATTTCCTTCA AGGAAATGAG AAAAGTGGTG GAGGTTACAT AGTTGTGGAC CCCATTTTAC 
     1321 GTGTTGGTGC TGATAATCAT GTGCTACCCT TGGACTGTGT TACTCTTCAG ACATTTTTAG 
     1381 CTAAGTGTTT GGGACCTTTT GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG 
     1441 GCTACAACAT GATTCATTTT ACCCCATTGC AGACTCTTGG ACTATCTAGG TCATGCTACT 
     1501 CCCTTGCCAA TCAGTTAGAA TTAAATCCTG ACTTTTCAAG ACCTAATAGA AAGTATACCT 
     1561 GGAATGATGT TGGACAGCTA GTGGAAAAAT TAAAAAAGGA ATGGAATGTT ATTTGTATTA 
     1621 CTGATGTTGT CTACAATCAT ACTGCTGCTA ATAGTAAATG GATCCAGGAA CATCCAGAAT 
     1681 GTGCCTATAA TCTTGTGAAT TCTCCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC 
     1741 TTTGGCGTTT CTCCTGTGAT GTTGCAGAAG GGAAATACAA AGAAAAGGGA ATACCTGCTT 
     1801 TGATTGAAAA TGATCACCAT ATGAATTCCA TCCGAAAAAT AATTTGGGAG GATATTTTTC 
     1861 CAAAGCTTAA ACTCTGGGAA TTTTTCCAAG TAGATGTCAA CAAAGCGGTT GAGCAATTTA 
     1921 GAAGACTTCT TACACAAGAA AATAGGCGAG TAACCAAGTC TGATCCAAAC CAACACCTTA 
     1981 CGATTATTCA AGATCCTGAA TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC 
     2041 TAACGACTTT CATACCACAT GACAAGGGGC CAGCAGCAAT TGAAGAATGC TGTAATTGGT 
     2101 TTCATAAAAG AATGGAGGAA TTAAATTCAG AGAAGCATCG ACTCATTAAC TATCATCAGG 
     2161 AACAGGCAGT TAATTGCCTT TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC 
     2221 CAAAACTAGG ACCTGTCACT AGAAAGCATC CTTTAGTTAC CAGGTATTTT ACTTTCCCAT 
     2281 TTGAAGAGAT AGACTTCTCC ATGGAAGAAT CTATGATTCA TCTGCCAAAT AAAGCTTGTT 
     2341 TTCTGATGGC ACACAATGGA TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC 
     2401 CGGGTTCAGA AGTTTACCTA AGGAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC 
     2461 GCTATGGGAA TAAACCAGAG GACTGTCCTT ATCTCTGGGC ACACATGAAA AAATACACTG 
     2521 AAATAACTGC AACTTATTTC CAGGGAGTAC GTCTTGATAA CTGCCACTCA ACACCTCTTC 
     2581 ACGTAGCTGA GTACATGTTG GATGCTGCTA GGAATTTGCA ACCCAATTTA TATGTAGTAG 
     2641 CTGAACTGTT CACAGGAAGT GAAGATCTGG ACAATGTCTT TGTTACTAGA CTGGGCATTA 
     2701 GTTCCTTAAT AAGAGAGGCA ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT 
     2761 ACCGATATGG AGGAGAACCT GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC 
     2821 CAGCTATTGC ACATGCCCTG TTTATGGATA TTACGCATGA TAATGAGTGT CCTATTGTGC 
     2881 ATAGATCAGC GTATGATGCT CTTCCAAGTA CTACAATTGT TTCTATGGCA TGTTGTGCTA 
     2941 GTGGAAGTAC AAGAGGCTAT GATGAATTAG TGCCTCATCA GATTTCAGTG GTTTCTGAAG 
     3001 AACGGTTTTA CACTAAGTGG AATCCTGAAG CATTGCCTTC AAACACAGGT GAAGTTAATT 
     3061 TCCAAAGCGG CATTATTGCA GCCAGGTGTG CTATCAGTAA ACTTCATCAG GAGCTTGGAG 
     3121 CCAAGGGTTT TATTCAGGTG TATGTGGATC AAGTTGATGA AGACATAGTG GCAGTAACAA 
     3181 GACACTCACC TAGCATCCAT CAGTCTGTTG TGGCTGTATC TAGAACTGCT TTCAGGAATC 
     3241 CCAAGACTTC ATTTTACAGC AAGGAAGTGC CTCAAATGTG CATCCCTGGC AAAATTGAAG 
     3301 AAGTAGTTCT TGAAGCTAGA ACTATTGAGA GAAACACGAA ACCTTATAGG AAGGATGAGA 
     3361 ATTCAATCAA TGGAACACCA GATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG 
     3421 AAAGTAAAAT TGTTAAACAA GCTGGAGTTG CCACAAAAGG GCCCAATGAA TATATTCAAG 
     3481 AAATAGAATT TGAAAACTTG TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC 
     3541 CACATGCACA AGTCGCTGTT GGAATTCTTC GAAATCATCT GACACAATTC AGTCCTCACT 
     3601 TTAAATCTGG CAGCCTAGCT GTTGACAATG CAGATCCTAT ATTAAAAATT CCTTTTGCTT 
     3661 CTCTTGCCTC CAGATTAACT TTGGCTGAGC TAAATCAGAT CCTTTACCGA TGTGAATCAG 
     3721 AAGAAAAGGA AGATGGTGGA GGGTGCTATG ACATACCAAA CTGGTCAGCC CTTAAATATG 
     3781 CAGGTCTTCA AGGTTTAATG TCTGTATTGG CAGAAATAAG ACCAAAGAAT GACTTGGGGC 
     3841 ATCCTTTTTG TAATAATTTG AGATCTGGAG ATTGGATGAT TGACTATGTC AGTAACCGGC 
     3901 TTATTTCACG ATCAGGAACT ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT 
     3961 ACCTGAAGCA GATCCCACGT TACCTTATCC CATGTTACTT TGATGCTATA TTAATTGGTG 
     4021 CATATACCAC TCTTCTGGAT ACAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT 
     4081 CAACCTTTGT GAAACACCTT TCATTGGGTT CAGTTCAACT GTGTGGAGTA GGAAAATTCC 
     4141 CTTCCCTGCC AATTCTTTCA CCTGCCCTAA TGGATGTACC TTATAGGTTA AATGAGATCA 
     4201 CAAAAGAAAA GGAGCAATGT TGTGTTTCTC TAGCTGCAGG CTTACCTCAT TTTTCTTCTG 
     4261 GTATTTTCCG CTGCTGGGGA AGGGATACTT TTATTGCACT TAGAGGTATA CTGCTGATTA 
     4321 CTGGACGCTA TGTAGAAGCC AGGAATATTA TTTTAGCATT TGCGGGTACC CTGAGGCATG 
     4381 GTCTCATTCC TAATCTACTG GGTGAAGGAA TTTATGCCAG ATACAATTGT CGGGATGCTG 
     4441 TGTGGTGGTG GCTGCAGTGT ATCCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA 
     4501 TTCTCAAGTG CCCAGTTTCC AGAATGTATC CTACAGATGA TTCTGCTCCT TTGCCTGCTG 
     4561 GCACACTGGA TCAGCCATTG TTTGAAGTCA TACAGGAAGC AATGCAAAAA CACATGCAGG 
     4621 GCATACAGTT CCGAGAAAGG AATGCTGGTC CCCAGATAGA TCGAAACATG AAGGACGAAG 
     4681 GTTTTAATAT AACTGCAGGA GTTGATGAAG AAACAGGATT TGTTTATGGA GGAAATCGTT 
     4741 TCAATTGTGG CACATGGATG GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA 
     4801 TCCCAGCCAC ACCAAGAGAT GGGTCTGCTG TGGAAATTGT GGGCCTGAGT AAATCTGCTG 
     4861 TTCGCTGGTT GCTGGAATTA TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCACAGTAA 
     4921 AAAGACATGG AAAGGCTATA AAGGTCTCAT ATGATGAGTG GAACAGAAAA ATACAAGACA 
     4981 ACTTTGAAAA GCTATTTCAT GTTTCCGAAG ACCCTTCAGA TTTAAATGAA AAGCATCCAA 
     5041 ATCTGGTTCA CAAACGTGGC ATATACAAAG ATAGTTATGG AGCTTCAAGT CCTTGGTGTG 
     5101 ACTATCAGCT CAGGCCTAAT TTTACCATAG CAATGGTTGT GGCCCCTGAG CTCTTTACTA 
     5161 CAGAAAAAGC ATGGAAAGCT TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTTGGCA 
     5221 TGAAAACTTT AGATCCAGAT GATATGGTTT ACTGTGGAAT TTATGACAAT GCATTAGACA 
     5281 ATGACAACTA CAATCTTGCT AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC 
     5341 CTATTGGGTA TTTTCTTCGT GCAAAATTAT ATTTTTCCAG ATTGATGGGC CCGGAGACTA 
     5401 CTGCAAAGAC TATAGTTTTG GTTAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA 
     5461 GATCCCCTTG GAAAGGACTT CCAGAACTGA CCAATGAGAA TGCCCAGTAC TGTCCTTTCA 
     5521 GCTGTGAAAC ACAAGCCTGG TCAATTGCTA CTATTCTTGA GACACTTTAT GATTTAACGC 
     5581 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5641 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5701 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5761 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5821 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5881 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5941 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     6001 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     6061 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6121 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6181 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6241 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6301 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6361 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6421 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6481 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6541 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6601 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6661 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6721 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6781 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6841 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6901 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6961 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7021 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7081 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7141 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7201 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7261 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7321 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7381 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7441 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7501 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7561 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7621 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7681 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7741 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7801 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7861 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7921 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7981 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     8041 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     8101 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8161 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8221 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8281 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8341 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8401 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8461 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8521 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8581 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8641 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8761 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8821 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     9001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     9061 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9121 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9181 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9241 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9361 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9421 GCGAATTTT
//